Small aminoacyl transfer centers at GU within a larger RNA
... surrounding sequences, activity remains detectable. In fact, aminoacylated product accumulation appears to be a sensitive monitor of structure at the site of the ribozyme, perhaps usable for this purpose in other experiments. Accordingly, these results suggest that higher order structure within larg ...
... surrounding sequences, activity remains detectable. In fact, aminoacylated product accumulation appears to be a sensitive monitor of structure at the site of the ribozyme, perhaps usable for this purpose in other experiments. Accordingly, these results suggest that higher order structure within larg ...
Molecular Cloning and Expression of a 2-Arylpropionyl
... from Sigma Chemie (Deisenhofen, Germany). Library screening and cloning. 2-arylpropionyl-CoA epimerase antibodies were obtained and purified as described previously (5). Approximately 6.0 3 105 plaques from a commercially available lgt11 cDNA library constructed from rat liver RNA (Clontech Laborato ...
... from Sigma Chemie (Deisenhofen, Germany). Library screening and cloning. 2-arylpropionyl-CoA epimerase antibodies were obtained and purified as described previously (5). Approximately 6.0 3 105 plaques from a commercially available lgt11 cDNA library constructed from rat liver RNA (Clontech Laborato ...
Metabolic decision making by protein-metabolite - ETH E
... contribution of specific transcription factors and global transcriptional regulation to E. coli’s gene expression. Using the L‐arginine biosynthesis pathway as an example, we show that global transcriptional regulation sets each promoter’s maximal capacity in a lar ...
... contribution of specific transcription factors and global transcriptional regulation to E. coli’s gene expression. Using the L‐arginine biosynthesis pathway as an example, we show that global transcriptional regulation sets each promoter’s maximal capacity in a lar ...
Variations in amino acid composition in bacterial single stranded
... tance/correlation of the Ct domain length for the SSB proteins from bacteria with high GC content. Recently, it was shown that shortening of C-terminal region of E. coli SSB protein had an impact on cooperative binding to ssDNA (16). It was also reported that extension of Ct domain slowed cell growt ...
... tance/correlation of the Ct domain length for the SSB proteins from bacteria with high GC content. Recently, it was shown that shortening of C-terminal region of E. coli SSB protein had an impact on cooperative binding to ssDNA (16). It was also reported that extension of Ct domain slowed cell growt ...
Journal of Bacteriology
... Biological N2 fixation is effected by the nitrogenase enzyme system, and its components have been isolated and purified from a variety of microorganisms. Nitrogenase consists of two 02-sensitive proteins, a molybdenum-iron protein (dinitrogenase) with two kinds of subunit and an iron-sulfur protein ...
... Biological N2 fixation is effected by the nitrogenase enzyme system, and its components have been isolated and purified from a variety of microorganisms. Nitrogenase consists of two 02-sensitive proteins, a molybdenum-iron protein (dinitrogenase) with two kinds of subunit and an iron-sulfur protein ...
Development and application of a positive
... transfection efficiencies and the low number of selectable markers. Presently, it is possible to stably transform four species of Plasmodium, the human parasite Plasmodium falciparum, the primate parasite Plasmodium knowlesi and two rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii ...
... transfection efficiencies and the low number of selectable markers. Presently, it is possible to stably transform four species of Plasmodium, the human parasite Plasmodium falciparum, the primate parasite Plasmodium knowlesi and two rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii ...
A method for measuring the non-random bias of a codon usage table
... usage table of a gene. The test is based on the multinomial and Poisson distributions. The method is used to scan DNA sequences and measure the strength of codon preference. For E. Coli we show that the strength of codon preference is related to levels of gene expression. The method can also be used ...
... usage table of a gene. The test is based on the multinomial and Poisson distributions. The method is used to scan DNA sequences and measure the strength of codon preference. For E. Coli we show that the strength of codon preference is related to levels of gene expression. The method can also be used ...
Gene Section SOX10 (SRY (sex determining region Y)-box 10)
... SOX10 is furthermore required for specification of melanocytes and peripheral glia from the neural crest. After specification, SOX10 continues to be essential for lineage progression and maintenance of identity in peripheral glia. Terminal differentiation of oligodendrocytes also depends on SOX10. S ...
... SOX10 is furthermore required for specification of melanocytes and peripheral glia from the neural crest. After specification, SOX10 continues to be essential for lineage progression and maintenance of identity in peripheral glia. Terminal differentiation of oligodendrocytes also depends on SOX10. S ...
Planta
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
Conceptual Questions C1. Answer: The start codon begins at the
... cloverleaf structure with three stem-loop structures. The second stem-loop contains the anticodon sequence that recognizes the codon sequence in mRNA. At the 3′ end, there is an acceptor stem, with the sequence CCA, that serves as an attachment site for an amino acid. Most tRNAs also have base modif ...
... cloverleaf structure with three stem-loop structures. The second stem-loop contains the anticodon sequence that recognizes the codon sequence in mRNA. At the 3′ end, there is an acceptor stem, with the sequence CCA, that serves as an attachment site for an amino acid. Most tRNAs also have base modif ...
BIO450 Primer Design Tutorial
... The annealing temperature for a PCR reaction is based on the melting temperature (Tm) of the primers bound to the templates. The Tm is the temperature at which a population of double stranded DNA molecules has half of the molecules in a single stranded state and half in the double stranded state. At ...
... The annealing temperature for a PCR reaction is based on the melting temperature (Tm) of the primers bound to the templates. The Tm is the temperature at which a population of double stranded DNA molecules has half of the molecules in a single stranded state and half in the double stranded state. At ...
Splicing together sister chromatids
... ring maintains sister chromatids together. The stabilization of these cohesin complexes depends on sororin (arrows), which prevents premature WAPL-dependent removal of cohesion from chromatin. These sororin-containing cohesin rings maintain sister chromatids together during mitosis (M) until cohesin ...
... ring maintains sister chromatids together. The stabilization of these cohesin complexes depends on sororin (arrows), which prevents premature WAPL-dependent removal of cohesion from chromatin. These sororin-containing cohesin rings maintain sister chromatids together during mitosis (M) until cohesin ...
E. coli
... The LT-IIb gene was also cloned and analysed. The A genes of LT-IIa and LT-IIb exhibited 71% DNA sequence homology with each other and 55 to 57% homology with the A genes of CT and LT-I. The B subunits of LT-IIa and LT-IIb differ from the LT-I in their carbohydrate-binding specificities. The ...
... The LT-IIb gene was also cloned and analysed. The A genes of LT-IIa and LT-IIb exhibited 71% DNA sequence homology with each other and 55 to 57% homology with the A genes of CT and LT-I. The B subunits of LT-IIa and LT-IIb differ from the LT-I in their carbohydrate-binding specificities. The ...
Skeletal muscle actin mRNA. Characterization of the 3
... was made by comparing their nucleotide sequences (Fig. 2). The insert of plasmid plO6 was completely sequenced (Fig. 2). The insert starts with a nucleotide t r i p l e t coding for phenylalanine (actin C-terminal residue), followed by a translation termination codon (TAG). I t contains 240 nucleoti ...
... was made by comparing their nucleotide sequences (Fig. 2). The insert of plasmid plO6 was completely sequenced (Fig. 2). The insert starts with a nucleotide t r i p l e t coding for phenylalanine (actin C-terminal residue), followed by a translation termination codon (TAG). I t contains 240 nucleoti ...
Bacterial physiological adaptations to contrasting edaphic
... – is part of the pentose phosphate pathway, a metabolic pathway parallel to glycolysis yielding pentose sugars and reducing agents. Though not previously considered largely with respect to soil functionality, this gene was generally of high abundance across all soils. However, novel position-specifi ...
... – is part of the pentose phosphate pathway, a metabolic pathway parallel to glycolysis yielding pentose sugars and reducing agents. Though not previously considered largely with respect to soil functionality, this gene was generally of high abundance across all soils. However, novel position-specifi ...
... carried out on ClustalW and Insight II (Asselryx) respectively. Results shown a membrane protein composed by eleven transmembrane helices containing 15 specific domains (GXXXG) which probably allows helix–helix interactions and dimerization. The presence of Glycine and Alanine residues inside this d ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
Mini-Series: Modern Metabolic Concepts The Biochemistry of the
... (PDHA-1 for human) located on the X chromosome and a testis-specific form with the gene (PDHA-2 for human) on chromosome 4 [24]. The second isoform is necessary during spermatogenesis when the X chromosome is inactivated or absent. A. suum has two E1 isoforms, isoform I in anaerobic adults and isofo ...
... (PDHA-1 for human) located on the X chromosome and a testis-specific form with the gene (PDHA-2 for human) on chromosome 4 [24]. The second isoform is necessary during spermatogenesis when the X chromosome is inactivated or absent. A. suum has two E1 isoforms, isoform I in anaerobic adults and isofo ...
Article Fitness Trade-Offs Determine the Role of the Molecular
... effect of mutations that affect protein folding. Unanswered questions about this buffering include the nature of its mechanism, its influence on the genetic variation of a population, the fitness trade-offs constraining this mechanism, and its role in expediting evolution. Answering these questions ...
... effect of mutations that affect protein folding. Unanswered questions about this buffering include the nature of its mechanism, its influence on the genetic variation of a population, the fitness trade-offs constraining this mechanism, and its role in expediting evolution. Answering these questions ...
Lesson Overview - Dr. Thornton`s Courses
... How can genetic engineering benefit agriculture and industry? Ideally, genetic modification could lead to better, less expensive, and more nutritious food as well as less harmful manufacturing processes. ...
... How can genetic engineering benefit agriculture and industry? Ideally, genetic modification could lead to better, less expensive, and more nutritious food as well as less harmful manufacturing processes. ...