Cloning and Sequence Analysis of the xylL Gene Responsible for
... restriction endonuclease and ligation of DNA fragments by T4 DNA ligase were performed by standard procedures as recommended by the supplier (Posco Co., Seoul, Korea). The pCS1 and pCSP21 carrying the xylL gene were previously cloned from the chromosomal DNA of Pseudomonas sp. S-47 (9). In this stud ...
... restriction endonuclease and ligation of DNA fragments by T4 DNA ligase were performed by standard procedures as recommended by the supplier (Posco Co., Seoul, Korea). The pCS1 and pCSP21 carrying the xylL gene were previously cloned from the chromosomal DNA of Pseudomonas sp. S-47 (9). In this stud ...
Epigenetic Changes in Foxp3 in Treg of Identical Twins Discordant
... indeed result in genetic alterations of individuals, based on the fact that there are identical twin pairs in which one twin has developed a disease while the other one has not. However, there is insufficient information on the mechanisms in which these epigenetic processes can occur, despite the fa ...
... indeed result in genetic alterations of individuals, based on the fact that there are identical twin pairs in which one twin has developed a disease while the other one has not. However, there is insufficient information on the mechanisms in which these epigenetic processes can occur, despite the fa ...
Prediction of mitochondrial proteins of malaria parasite
... annotate mitochondrial proteins. Mitochondria in plasmodium parasites have many characteristics that distinguish them from mammalian mitochondria. Mitochondrial proteins of PF are different than human mitochondrial proteins; this makes PF mitochondrial protein as attractive potential drug targets (V ...
... annotate mitochondrial proteins. Mitochondria in plasmodium parasites have many characteristics that distinguish them from mammalian mitochondria. Mitochondrial proteins of PF are different than human mitochondrial proteins; this makes PF mitochondrial protein as attractive potential drug targets (V ...
Supplements - Haiyuan Yu
... into the next conversion algorithm. Conversion algorithms associated with each edge vary throughout the graph, and must take into account different parameters depending on the type of conversion being made. For instance, converting from a genomic locus to transcript locus follows inherently differen ...
... into the next conversion algorithm. Conversion algorithms associated with each edge vary throughout the graph, and must take into account different parameters depending on the type of conversion being made. For instance, converting from a genomic locus to transcript locus follows inherently differen ...
Thermodynamic analysis of DNA binding by a Bacillus single
... multi-protein complexes required for processes such as DNA replication, recombination and DNA repair [8-11]. The function of SSB during DNA replication has been extensively studied in E. coli, which serves as the prototypical model system for prokaryotes and eukaryotes alike. In E. coli, the large n ...
... multi-protein complexes required for processes such as DNA replication, recombination and DNA repair [8-11]. The function of SSB during DNA replication has been extensively studied in E. coli, which serves as the prototypical model system for prokaryotes and eukaryotes alike. In E. coli, the large n ...
New insight into plant intramembrane proteases
... The 31 sequences were assigned to the secretase B clade. The average sequence conservation within these group was 34% and the average identity of their rhomboid domains was 44%. Within this group 14 sequences represented monocots and 17 sequences represented dicots. The number of sequences identifie ...
... The 31 sequences were assigned to the secretase B clade. The average sequence conservation within these group was 34% and the average identity of their rhomboid domains was 44%. Within this group 14 sequences represented monocots and 17 sequences represented dicots. The number of sequences identifie ...
biology - Kendriya Vidyalaya No.1 Kanchrapara
... 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types of lymphocytes in humans . How do they differ in their roles in immune ...
... 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types of lymphocytes in humans . How do they differ in their roles in immune ...
Rhizopus Raw-Starch-Degrading Glucoamylase: Its
... CDNAlibrary prepared from Rhizopus mRNA.Sequence analysis of both glucoamylase genes revealed that the genomic gene contained 4 intervening sequences and the CDNAgene lacked 145 nucleotides corresponding to the N-terminal region. The glucoamylase consists of 604 amino acids including a putative sign ...
... CDNAlibrary prepared from Rhizopus mRNA.Sequence analysis of both glucoamylase genes revealed that the genomic gene contained 4 intervening sequences and the CDNAgene lacked 145 nucleotides corresponding to the N-terminal region. The glucoamylase consists of 604 amino acids including a putative sign ...
Open the publication - UEF Electronic Publications
... Gene sets were introduced to overcome the challenges that focusing to a few genes brought up. Gene sets are group of genes sharing the same function, defined based on prior knowledge, such as biological pathway. The approach was developed to discover differences between two distinct phenotypes, such ...
... Gene sets were introduced to overcome the challenges that focusing to a few genes brought up. Gene sets are group of genes sharing the same function, defined based on prior knowledge, such as biological pathway. The approach was developed to discover differences between two distinct phenotypes, such ...
Biomolecular chemistry 3. Translating the genetic code
... • The genetic code is the relation between the sequence of bases in DNA (or its RNA transcripts) and the sequence of amino acids in proteins. Experiments by Francis Crick, Sydney Brenner, and others established the following features of the genetic code by 1961: • Three nucleotides encode an amino a ...
... • The genetic code is the relation between the sequence of bases in DNA (or its RNA transcripts) and the sequence of amino acids in proteins. Experiments by Francis Crick, Sydney Brenner, and others established the following features of the genetic code by 1961: • Three nucleotides encode an amino a ...
PhytoREF: a reference database of the plastidial 16S
... of photosynthetic eukaryotes in the natural environment using classical microscopy-based techniques. For most taxa, taxonomic identification is greatly hindered by their minute size (as small as 0.8 µm for the prasinophyte Ostreococcus; Courties et al. 1994; Vaulot et al. 2008), lack of distinctive ...
... of photosynthetic eukaryotes in the natural environment using classical microscopy-based techniques. For most taxa, taxonomic identification is greatly hindered by their minute size (as small as 0.8 µm for the prasinophyte Ostreococcus; Courties et al. 1994; Vaulot et al. 2008), lack of distinctive ...
Identification of the tRNA-binding Protein Arc1p as a Novel Target of
... Yeast Strains, Vectors, and Media—The S. cerevisiae strains used in this study are listed in Table I. For recombinant plasmid constructions, the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as ...
... Yeast Strains, Vectors, and Media—The S. cerevisiae strains used in this study are listed in Table I. For recombinant plasmid constructions, the S. cerevisiae/E. coli shuttle vectors YCplac33 (14), p414MET25 (15), pVT-100U (16), pTRC-HISA (Invitrogen) and the E. coli plasmid pQE70 (Qiagen) served as ...
R26 :: CAG GCaMP6f - The Jackson Laboratory
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
... international biomedical research community - adds hundreds of new strains annually. The JAX Mouse Repository is supported by NIH, The Howard Hughes Medical Institute and several private charitable foundations. ...
Gel Electrophoresis
... commonly used as a fluorescent tag (nucleic acid stain) mixed with the gel during its preparation. It is commonly abbreviated as "EtBr. When exposed to ultraviolet light. It will fluoresce with an orange colour ...
... commonly used as a fluorescent tag (nucleic acid stain) mixed with the gel during its preparation. It is commonly abbreviated as "EtBr. When exposed to ultraviolet light. It will fluoresce with an orange colour ...
Question bank in Biology class XII
... 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types of lymphocytes in humans . How do they differ in their roles in immune ...
... 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types of lymphocytes in humans . How do they differ in their roles in immune ...
... it is in the hydrophobic core. Since this Thr would form an H-bond to water in the unfolded state, it will cost about 20 kJ/mole of instability since a hydrogen bond to water is broken during folding, but not reformed. (6 pts) Additional factors (1 pt for either, unless the main reason was missed an ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... Bacillus phage29. The similarity between these conserved regions is
most striking in their linear spatial arrangement on each pol polypeptide.
The regions are designated I-VI by their extent of similarity, with region
I being the most conserved (Wong et al. 1988). Delarue et al. designated
the t ...
... Bacillus phage
Origin of amino acid homochirality: Relationship with the RNA world
... (Fig. 2). The rationale was that since the free energy of aminoacyl phosphates hydrolysis is approximately 3 kcal mol−1 greater than that of the aminoacyl esters (aminoacyl-tRNAs) (Carpenter, 1960), aminoacylation would be spontaneous. This system aminoacylated the minihelix at the 3 -end and the c ...
... (Fig. 2). The rationale was that since the free energy of aminoacyl phosphates hydrolysis is approximately 3 kcal mol−1 greater than that of the aminoacyl esters (aminoacyl-tRNAs) (Carpenter, 1960), aminoacylation would be spontaneous. This system aminoacylated the minihelix at the 3 -end and the c ...
The Proteins of the Keratin Component of Bird`s Beaks
... Charge heterogeneity of the proteins was studied by electrophoresis on cellulose acetate in urea at pH 8·9 (Blagrove et al. 1975) and in urea (6 M), citric acid (0·2 M) and sodium chloride (0·03 M) at pH2·9. Electrophoresis of the S-carboxymethyl proteins in sodium dodecyl sulphate (SDS)-polyacrylam ...
... Charge heterogeneity of the proteins was studied by electrophoresis on cellulose acetate in urea at pH 8·9 (Blagrove et al. 1975) and in urea (6 M), citric acid (0·2 M) and sodium chloride (0·03 M) at pH2·9. Electrophoresis of the S-carboxymethyl proteins in sodium dodecyl sulphate (SDS)-polyacrylam ...
PrionPPSatBlack
... Examples of questions that can be explored through structural analyses Some mutations have been associated with prion diseases. By mapping these onto structures, propose a hypothesis to explain this. Most of the variation seems to be in the Nterminal region. Is there homology to this region in othe ...
... Examples of questions that can be explored through structural analyses Some mutations have been associated with prion diseases. By mapping these onto structures, propose a hypothesis to explain this. Most of the variation seems to be in the Nterminal region. Is there homology to this region in othe ...
Adaptative biochemical pathways and regulatory networks in
... Physiological studies [12], EPS composition and metal-binding activity thereof [17, 18] revealed characteristic peculiarities of BAS-10 strain. Thus, a sequence of 1447 nt gene was generated from BAS-10 16S rDNA (Additional File 1) to perform phylogenetic clustering. Two ClustalW analyses were perfo ...
... Physiological studies [12], EPS composition and metal-binding activity thereof [17, 18] revealed characteristic peculiarities of BAS-10 strain. Thus, a sequence of 1447 nt gene was generated from BAS-10 16S rDNA (Additional File 1) to perform phylogenetic clustering. Two ClustalW analyses were perfo ...
Structural and functional study of K453E mutant protective
... According to the crystal structure reported by Rudenko et al. (1995), human PPCA consists of “core” and “cap” domains. The core domain contains ten central β-sheets, and an additional ten α-helices and two small β-sheets occur on both sides of the central β-sheets. The cap domain is divided into a “ ...
... According to the crystal structure reported by Rudenko et al. (1995), human PPCA consists of “core” and “cap” domains. The core domain contains ten central β-sheets, and an additional ten α-helices and two small β-sheets occur on both sides of the central β-sheets. The cap domain is divided into a “ ...