Exploring Prostate Proteins and Antibodies
... Which proteins are expressed in the prostate? To acknowledge Prostate Cancer Awareness Month, we take a closer look at the human prostate specific proteome. We explore the Human Protein Atlas's analysis of the prostate specific transcriptome, and present selected antibodies against proteins specific ...
... Which proteins are expressed in the prostate? To acknowledge Prostate Cancer Awareness Month, we take a closer look at the human prostate specific proteome. We explore the Human Protein Atlas's analysis of the prostate specific transcriptome, and present selected antibodies against proteins specific ...
Bioinformatics of proteins: Sequence, structure and the `symbiosis
... Same major secondary structures in the same arrangement and with the same topological connections. Different proteins with the same fold often have peripheral elements of secondary structure and turn regions that differ in size and conformation. In some cases, these differing peripheral regions may ...
... Same major secondary structures in the same arrangement and with the same topological connections. Different proteins with the same fold often have peripheral elements of secondary structure and turn regions that differ in size and conformation. In some cases, these differing peripheral regions may ...
Internal expression of Yarrowia NDH2
... spores carrying these deletion alleles, either by random spore analysis or by ascus dissection. As we also failed to introduce the deletion alleles directly into haploid yeast strains, it seemed that complex I was essential for vegetative growth. However, we could not exclude at this point that comp ...
... spores carrying these deletion alleles, either by random spore analysis or by ascus dissection. As we also failed to introduce the deletion alleles directly into haploid yeast strains, it seemed that complex I was essential for vegetative growth. However, we could not exclude at this point that comp ...
Drug-Resistant Variants of Escherichia coli Thymidylate Synthase
... CH2H4PteGlu correlates well with Ki values obtained for three TS-directed inhibitors. These results show that mutations at Pro-254 specifically affect the initial binding interactions between enzyme and cofactor and also alter the ability of the mutant enzymes to undergo conformational changes that ...
... CH2H4PteGlu correlates well with Ki values obtained for three TS-directed inhibitors. These results show that mutations at Pro-254 specifically affect the initial binding interactions between enzyme and cofactor and also alter the ability of the mutant enzymes to undergo conformational changes that ...
Slide
... Assuming uniform distribution of reads, C=10 results in 1 gapped region /1,000,000 nucleotides ...
... Assuming uniform distribution of reads, C=10 results in 1 gapped region /1,000,000 nucleotides ...
Transfer of genetic material between the chloroplast and nucleus
... identified (Arabidopsis Genome Initiative, 2000). These genes missing from the arabidopsis chloroplast genome are presumed to represent organelle-to-nuclear gene transfers that have occurred sometime after the divergence of the organelle-containing lineages. The completion of the arabidopsis genome ...
... identified (Arabidopsis Genome Initiative, 2000). These genes missing from the arabidopsis chloroplast genome are presumed to represent organelle-to-nuclear gene transfers that have occurred sometime after the divergence of the organelle-containing lineages. The completion of the arabidopsis genome ...
3.2 Breeding of corn line DAS-40278-9
... quizalofop-P-ethyl. This has been achieved through the introduction of the aad-1 gene, from Sphingobium herbicidovorans, expressing the enzyme aryloxyalkanoate dioxygenase (AAD-1). In conducting a safety assessment of food derived from herbicide-tolerant corn line DAS-40278-9, a number of criteria h ...
... quizalofop-P-ethyl. This has been achieved through the introduction of the aad-1 gene, from Sphingobium herbicidovorans, expressing the enzyme aryloxyalkanoate dioxygenase (AAD-1). In conducting a safety assessment of food derived from herbicide-tolerant corn line DAS-40278-9, a number of criteria h ...
SAMHD1 is a single-stranded nucleic acid
... that SAMHD1 binds ssDNA and ssRNA, but not duplex DNA or duplex RNA/DNA hybrids (18–20), there have been multiple and conflicting reports as to whether SAMHD1 has 3 -5 exonuclease activity (7,18,19,21). Notably, Beloglazova et al. reported that SAMHD1 exhibited a major 3 -5 ssDNA exonuclease act ...
... that SAMHD1 binds ssDNA and ssRNA, but not duplex DNA or duplex RNA/DNA hybrids (18–20), there have been multiple and conflicting reports as to whether SAMHD1 has 3 -5 exonuclease activity (7,18,19,21). Notably, Beloglazova et al. reported that SAMHD1 exhibited a major 3 -5 ssDNA exonuclease act ...
TFIIH Plays an Essential Role in RNA Polymerase I Transcription
... of Pol I and TIF-IB Mammalian Pol I is heterogeneous with regard to chromatographic properties, electrophoretic mobility, and ability to support specific transcription of rDNA (reviewed in Grummt, 1999). The initiation-competent form of Pol I is associated with accessory proteins that contribute to ...
... of Pol I and TIF-IB Mammalian Pol I is heterogeneous with regard to chromatographic properties, electrophoretic mobility, and ability to support specific transcription of rDNA (reviewed in Grummt, 1999). The initiation-competent form of Pol I is associated with accessory proteins that contribute to ...
REGULATION BY EXERCISE OF SKELETAL MUSCLE CONTENT
... (35, 40), in skeletal muscle, both in rodents and humans. It was generally assumed that this increase in PGC-1α protein mediates the increase in mitochondrial biogenesis that follows a bout of exercise. However, an examination of the time course of the adaptive response showed that the mRNA levels o ...
... (35, 40), in skeletal muscle, both in rodents and humans. It was generally assumed that this increase in PGC-1α protein mediates the increase in mitochondrial biogenesis that follows a bout of exercise. However, an examination of the time course of the adaptive response showed that the mRNA levels o ...
Table of Contents
... extend long templates in a fraction of the time, making Phusion a superior choice for cloning. Phusion is suitable for all PCR applications requiring greater accuracy or long amplicons. 3. Have the formulations or any other characteristics of these products changed now that they are manufactured by ...
... extend long templates in a fraction of the time, making Phusion a superior choice for cloning. Phusion is suitable for all PCR applications requiring greater accuracy or long amplicons. 3. Have the formulations or any other characteristics of these products changed now that they are manufactured by ...
Evolution of Brachyury proteins: identification of a novel regulatory
... Orthologues of Brachyury, a subfamily of T-box transcription factors, specify distinct cell types in different metazoan phyla, suggesting that the function of these genes has changed through the course of evolution. To investigate this evolutionary process, we have compared the activities of Brachyu ...
... Orthologues of Brachyury, a subfamily of T-box transcription factors, specify distinct cell types in different metazoan phyla, suggesting that the function of these genes has changed through the course of evolution. To investigate this evolutionary process, we have compared the activities of Brachyu ...
Anaerobic protists and hidden mitochondria
... cluster biosynthetic pathway and that these proteins target the proposed relict organelle. Thus, C. parvum is the latest to join the growing numbers that support the view that there are no truly amitochondriate extant eukaryotes (Müller, 2000). The report of mitochondrial-type hsp70 genes in two mi ...
... cluster biosynthetic pathway and that these proteins target the proposed relict organelle. Thus, C. parvum is the latest to join the growing numbers that support the view that there are no truly amitochondriate extant eukaryotes (Müller, 2000). The report of mitochondrial-type hsp70 genes in two mi ...
Increase in Tomato Locule Number Is Controlled by Two Single
... A number of genes controlling tomato fruit morphology have been cloned: FW2.2 (Frary et al., 2000) controls fruit weight; SUN (Xiao et al., 2008) and OVATE (Liu et al., 2002) control elongated fruit shape; and FASCIATED (FAS; Cong et al., 2008) controls locule number, fruit size, and flat fruit shap ...
... A number of genes controlling tomato fruit morphology have been cloned: FW2.2 (Frary et al., 2000) controls fruit weight; SUN (Xiao et al., 2008) and OVATE (Liu et al., 2002) control elongated fruit shape; and FASCIATED (FAS; Cong et al., 2008) controls locule number, fruit size, and flat fruit shap ...
sequence alignments
... • Synonymous mutations alter DNA but not amino acid sequences • Nonsynonymous mutations alter amino acid sequence ...
... • Synonymous mutations alter DNA but not amino acid sequences • Nonsynonymous mutations alter amino acid sequence ...
Gel electrophoresis - Caltech Particle Theory
... first want to get them to be linear so that the proteins no longer have any secondary, tertiary or quaternary structure (i.e. we want them to have the same linear shape). Consider two proteins that are each 500 amino acids long but one is shaped like a closed umbrella whle the other one looks like a ...
... first want to get them to be linear so that the proteins no longer have any secondary, tertiary or quaternary structure (i.e. we want them to have the same linear shape). Consider two proteins that are each 500 amino acids long but one is shaped like a closed umbrella whle the other one looks like a ...
K 2
... was found to be more prevalent than K1.this is in agreement with [17] who have indicated that K2 was more predominant in human infections but is very rarely identified in the natural environment. In addition to that, three isolates were found to have K1, and K2 and these isolates are considered as h ...
... was found to be more prevalent than K1.this is in agreement with [17] who have indicated that K2 was more predominant in human infections but is very rarely identified in the natural environment. In addition to that, three isolates were found to have K1, and K2 and these isolates are considered as h ...
Detailed proteome analysis of growing cells of the planctomycete
... ORFs), including 261 proteins with a predicted signal peptide. The identified proteins were functionally categorized using Clusters of Orthologous Groups (COGs), and their potential cellular locations were predicted by bioinformatic tools. A unique protein family that contains several YTV domains an ...
... ORFs), including 261 proteins with a predicted signal peptide. The identified proteins were functionally categorized using Clusters of Orthologous Groups (COGs), and their potential cellular locations were predicted by bioinformatic tools. A unique protein family that contains several YTV domains an ...
A Histone H3.3-like Gene Specifically Expressed in the Vegetative
... Drastic changes in gene expression patterns occur during pollen development in flowering plants (Mascarenhas 1990, Becker et al. 2003, Honys and Twell 2003, McCormick 2004). In particular, recent microarray analyses in Arabidopsis have revealed unique characteristics of the pollen transcriptome (Bec ...
... Drastic changes in gene expression patterns occur during pollen development in flowering plants (Mascarenhas 1990, Becker et al. 2003, Honys and Twell 2003, McCormick 2004). In particular, recent microarray analyses in Arabidopsis have revealed unique characteristics of the pollen transcriptome (Bec ...
Arabidopsis Contains Nine Long-Chain Acyl
... however, also contained a 45-amino acid residue region not found in the luciferase enzyme. This domain is located directly in between LS1 and LS2 and links the two luciferase-like domains. A linker domain of similar length was also observed in four of the homologous enzymes from B. napus (Fulda et a ...
... however, also contained a 45-amino acid residue region not found in the luciferase enzyme. This domain is located directly in between LS1 and LS2 and links the two luciferase-like domains. A linker domain of similar length was also observed in four of the homologous enzymes from B. napus (Fulda et a ...
Statistical analysis of DNA microarray data
... Proteomics, the study of the proteome, has largely been practiced through the separation of proteins by two dimensional gel electrophoresis. In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In the second dimension, proteins a ...
... Proteomics, the study of the proteome, has largely been practiced through the separation of proteins by two dimensional gel electrophoresis. In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In the second dimension, proteins a ...
Colicins produced by the Escherichia fergusonii strains closely
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
A complete shikimate pathway in Toxoplasma gondii: an ancient
... The tBLASTn alogrithim was used to search ToxoDB 2.1 for evidence of both Class I or/and II DAHP synthases. A portion of Contig TGG_9597 was found to code for a putative protein with similarity to Class II DAHP synthase, but no likely candidates were identified for a Class I DAHP synthase. This regi ...
... The tBLASTn alogrithim was used to search ToxoDB 2.1 for evidence of both Class I or/and II DAHP synthases. A portion of Contig TGG_9597 was found to code for a putative protein with similarity to Class II DAHP synthase, but no likely candidates were identified for a Class I DAHP synthase. This regi ...
Identification and functional analysis of a prokaryotic-type
... further investigate the properties and functional characteristics of the PT-AAT in plants. Samples containing recombinant PpAAT and total protein extracts from pine cotyledons, hypocotyls and roots were analysed by Western blotting (Figure 5a). Anti-PpAAT antiserum specifically recognized decreasing ...
... further investigate the properties and functional characteristics of the PT-AAT in plants. Samples containing recombinant PpAAT and total protein extracts from pine cotyledons, hypocotyls and roots were analysed by Western blotting (Figure 5a). Anti-PpAAT antiserum specifically recognized decreasing ...
Conservation and relative importance of residues across protein
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...