Strategies in the interfield discovery of the mechanism of protein
... story (mostly its biochemical side) that we will not discuss. However, we will weave together some of the new historical work by Rheinberger (1997) concerning Paul Zamecnik’s biochemical research with more familiar accounts of molecular biological work. Rheinberger’s emphasis on Zamecnik’s ‘experime ...
... story (mostly its biochemical side) that we will not discuss. However, we will weave together some of the new historical work by Rheinberger (1997) concerning Paul Zamecnik’s biochemical research with more familiar accounts of molecular biological work. Rheinberger’s emphasis on Zamecnik’s ‘experime ...
Leishmania donovani - Oxford Academic
... that normally contains the active site tyrosine within a conserved SKXXY motif (15). Prediction analysis using NetPhos indicated that within the C-terminal domain of this parasite protein, serine residues and not tyrosine had maximum probability of acting as potential phosphorylation sites for enzym ...
... that normally contains the active site tyrosine within a conserved SKXXY motif (15). Prediction analysis using NetPhos indicated that within the C-terminal domain of this parasite protein, serine residues and not tyrosine had maximum probability of acting as potential phosphorylation sites for enzym ...
TRANSLATION: How to make proteins?
... mRNA t1/2 = few minutes to 2 hours (yeast) to >90 hours (mammals) ...
... mRNA t1/2 = few minutes to 2 hours (yeast) to >90 hours (mammals) ...
Biology: semester one: course outline
... relate chemical and physical processes to the metabolism of the cell Understand similarities and differences between cellular respiration and fermentation Consider how physical, chemical and biological processes work together in the process of photosynthesis Relate chemical signalling to cellular re ...
... relate chemical and physical processes to the metabolism of the cell Understand similarities and differences between cellular respiration and fermentation Consider how physical, chemical and biological processes work together in the process of photosynthesis Relate chemical signalling to cellular re ...
RNA 3`-terminal phosphate cyclases and cyclase
... substrate specificity of RtcA even further. They demonstrated that at the slow rate the E. coli RtcA can convert, in a reaction involving an RctA-AMP covalent intermediate, the 5’-phosphates in RNA and DNA into A5’pp5’N structures. This modification optimally occurs at pH of 5.5-6.0, in contrast wit ...
... substrate specificity of RtcA even further. They demonstrated that at the slow rate the E. coli RtcA can convert, in a reaction involving an RctA-AMP covalent intermediate, the 5’-phosphates in RNA and DNA into A5’pp5’N structures. This modification optimally occurs at pH of 5.5-6.0, in contrast wit ...
ABSTRACT Title of Document:
... Escherichia coli as commensal and model pathogen ............................................... 2 Extraintestinal Pathogenic Escherichia coli (ExPEC).............................................. 3 Virulence factors associated with ExPECs ............................................................ ...
... Escherichia coli as commensal and model pathogen ............................................... 2 Extraintestinal Pathogenic Escherichia coli (ExPEC).............................................. 3 Virulence factors associated with ExPECs ............................................................ ...
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... 1. Actively translating proteins were labeled with radioactive amino acids for a brief time (short relative to the time required to complete synthesis). 2. Completed polypeptides were collected, digested with trypsin, and the amount of radioactivity in tryptic fragments was determined. 3. Tryptic fr ...
... 1. Actively translating proteins were labeled with radioactive amino acids for a brief time (short relative to the time required to complete synthesis). 2. Completed polypeptides were collected, digested with trypsin, and the amount of radioactivity in tryptic fragments was determined. 3. Tryptic fr ...
... Rabies virus IS the prototype VIruS of the genus Lyssavirus, and has a large geographic distribution in Africa and Europe (King and Crick, 1988; Bourhy et aI., 1993). Control of the disease and epidemiological monitoring of the virus have histOlically been the main focus of rabies virus research. Th ...
Poon, Andy: Predicting Phosphorylation: A critique of the NetPhos program and potential alternatives
... initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases reveal that all phosphoproteins have a helix-loop-helix motif.) However, neither of the original BLOCKS database nor eMatrix identified any known motifs which ar ...
... initially attributed to other protein features, which may be indicative of phosphorylation as well. (For instance, perhaps these databases reveal that all phosphoproteins have a helix-loop-helix motif.) However, neither of the original BLOCKS database nor eMatrix identified any known motifs which ar ...
D-Isonucleotide (isoNA) incorporation around cleavage site of
... process or other steps. Therefore, the influence of modification with isoNA on the kinetics of passenger strand cleavage induced by T. thermophilus Ago (Tt-Ago) was evaluated. Previous reports studied the cleavage kinetics of the passenger strand with a 5′-32P-radiolabeled RNA oligonucleotide.11 The ...
... process or other steps. Therefore, the influence of modification with isoNA on the kinetics of passenger strand cleavage induced by T. thermophilus Ago (Tt-Ago) was evaluated. Previous reports studied the cleavage kinetics of the passenger strand with a 5′-32P-radiolabeled RNA oligonucleotide.11 The ...
Highly Efficient and Specific Multiplex PCR usingTaKaRa
... TaKaRa Taq DNA Polymerase Hot Start Version contains a mixture of highpurity TaKaRa Taq DNA Polymerase and a monoclonal antibody to Taq DNA Polymerase, which binds to the polymerase until the temperature is elevated. The binding of this antibody prevents nonspecific amplification due to mispriming ...
... TaKaRa Taq DNA Polymerase Hot Start Version contains a mixture of highpurity TaKaRa Taq DNA Polymerase and a monoclonal antibody to Taq DNA Polymerase, which binds to the polymerase until the temperature is elevated. The binding of this antibody prevents nonspecific amplification due to mispriming ...
GLUCOCORTICOID AND MINERALOCORTICOID RECEPTORS
... MR not only have equivalent affinity for aldosterone and cortisol, they are also expressed at considerable levels in tissues such as hippocampus and heart (1, 26), which are not classical aldosterone targets. The question of how aldosterone can occupy MR in epithelial target tissues has been answere ...
... MR not only have equivalent affinity for aldosterone and cortisol, they are also expressed at considerable levels in tissues such as hippocampus and heart (1, 26), which are not classical aldosterone targets. The question of how aldosterone can occupy MR in epithelial target tissues has been answere ...
Biomolecular chemistry 3. Translating the genetic code
... harmful and sometimes beneficial). Thus, degeneracy minimizes the deleterious effects of mutations. Degeneracy of the code may also be significant in permitting DNA base composition to vary over a wide range without altering the amino acid sequence of the proteins encoded by the DNA. The G + C conte ...
... harmful and sometimes beneficial). Thus, degeneracy minimizes the deleterious effects of mutations. Degeneracy of the code may also be significant in permitting DNA base composition to vary over a wide range without altering the amino acid sequence of the proteins encoded by the DNA. The G + C conte ...
Food derived from glufosinate ammonium
... Tu494. It codes for the enzyme phosphinothricin acetyl transferase (PAT), which modifies and inactivates the herbicide glufosinate ammonium (Strauch et al, 1988). The pat gene is often used as a selectable marker to distinguish genetically modified plant cells from unmodified cells. In this applicat ...
... Tu494. It codes for the enzyme phosphinothricin acetyl transferase (PAT), which modifies and inactivates the herbicide glufosinate ammonium (Strauch et al, 1988). The pat gene is often used as a selectable marker to distinguish genetically modified plant cells from unmodified cells. In this applicat ...
The hnRNP C Proteins Contain a Nuclear Retention Sequence That
... which has a myc epitope (MGEQKLISEEDL) just upstream of the EcoRI site (Siomi and Dreyfuss, 1995). Myc-C1 was made by PCR amplification of the C1 cDNA pHC12 (Swanson et al., 1987) to generate at the 5' end an EcoRI site immediately upstream of the second codon of C1, and at the 3' end an XhoI site d ...
... which has a myc epitope (MGEQKLISEEDL) just upstream of the EcoRI site (Siomi and Dreyfuss, 1995). Myc-C1 was made by PCR amplification of the C1 cDNA pHC12 (Swanson et al., 1987) to generate at the 5' end an EcoRI site immediately upstream of the second codon of C1, and at the 3' end an XhoI site d ...
Supplementary information for Ronshaugen, McGinnis
... determined for the wild type A1 segments. Identically treated embryos were then stained with a rat monoclonal anti-HA antibody and the mean luminosity with this antibody in the T2 segment was determined. All other transgenic lines were similarly assayed using the anti-HA antibody. These were selecte ...
... determined for the wild type A1 segments. Identically treated embryos were then stained with a rat monoclonal anti-HA antibody and the mean luminosity with this antibody in the T2 segment was determined. All other transgenic lines were similarly assayed using the anti-HA antibody. These were selecte ...
34750 - Radboud Repository
... from intermediate metabolism, such as synthesis of heme groups [1], steroids [2], amino acids, and iron-sulphur (Fe-S) clusters [3]. Phylogenetic analyses of mitochondrial genes indicate that all mitochondria derive from a single alphaproteobacterial ancestor, the so-called proto-mitochondrion [4]. ...
... from intermediate metabolism, such as synthesis of heme groups [1], steroids [2], amino acids, and iron-sulphur (Fe-S) clusters [3]. Phylogenetic analyses of mitochondrial genes indicate that all mitochondria derive from a single alphaproteobacterial ancestor, the so-called proto-mitochondrion [4]. ...
Evolutionary Forces in Shaping the Codon and Amino Acid Usages
... one codon is used per amino acid) to 61 (when all codons are used in equal probability). The expected value of Nc under random codon usage is given by the following formula: Nc = 2 + s + {29/[s2 + (1-s)2]}; Where s = GC3s All the parameters were calculated by using the programme CodonW 1.3 (availabl ...
... one codon is used per amino acid) to 61 (when all codons are used in equal probability). The expected value of Nc under random codon usage is given by the following formula: Nc = 2 + s + {29/[s2 + (1-s)2]}; Where s = GC3s All the parameters were calculated by using the programme CodonW 1.3 (availabl ...
RECOMBINANT-DNA METHODOLOGY
... wild-type sequence but does not cut the mutant sequence (or vice versa). A restriction site right at the site of the mutation would come in handy. If the fetal DNA has the normal sequence, the DNA will be cut and the restriction pattern will be identical to the wild type. If not, not. For many genet ...
... wild-type sequence but does not cut the mutant sequence (or vice versa). A restriction site right at the site of the mutation would come in handy. If the fetal DNA has the normal sequence, the DNA will be cut and the restriction pattern will be identical to the wild type. If not, not. For many genet ...
RNA-binding proteins and RNA metabolism: a new scenario in the
... up-regulated, respectively, and both miRNAs were capable of binding directly to TDP-43. These TDP43-miRNA interactions have been also shown to affect the expression levels of candidate cellular transcripts, such as the cyclin-dependent kinase 6 (Cdk6), which was previously found to be up-regulated f ...
... up-regulated, respectively, and both miRNAs were capable of binding directly to TDP-43. These TDP43-miRNA interactions have been also shown to affect the expression levels of candidate cellular transcripts, such as the cyclin-dependent kinase 6 (Cdk6), which was previously found to be up-regulated f ...