White biotechnology report RIVM
... ties offered by new techniques and approaches. These can be used for enhancing the effi‐ ciency of metabolic processes that are already in use, or to devise novel metabolic processes for the production of a large variety of biological compounds. There is a clear distinction be‐ tween the expectatio ...
... ties offered by new techniques and approaches. These can be used for enhancing the effi‐ ciency of metabolic processes that are already in use, or to devise novel metabolic processes for the production of a large variety of biological compounds. There is a clear distinction be‐ tween the expectatio ...
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... “coevolutionary” theory in which amino acids and (very small) peptides as well as small RNAs existed together and where their separate abilities not only reinforced each other’s survival, but allowed life to more quickly climb the ladder of complexity. Essential for our approach is the following: St ...
... “coevolutionary” theory in which amino acids and (very small) peptides as well as small RNAs existed together and where their separate abilities not only reinforced each other’s survival, but allowed life to more quickly climb the ladder of complexity. Essential for our approach is the following: St ...
Metabolomics based gene function annotation in Escherichia coli
... mutants. Additionally, metabolomics can be used to explore metabolic diversity of different accessions/strains, tissues, and cell types of an organism. Once the metabolic diversity has been described, the genetic-basis for the metabolic and/or phenotypic differences can be elucidated using other omi ...
... mutants. Additionally, metabolomics can be used to explore metabolic diversity of different accessions/strains, tissues, and cell types of an organism. Once the metabolic diversity has been described, the genetic-basis for the metabolic and/or phenotypic differences can be elucidated using other omi ...
Biomolecular chemistry 3. Translating the genetic code
... The fidelity of protein synthesis requires the accurate recognition of three-base codons on messenger RNA. Recall that the genetic code relates each amino acid to a three-letter codon. A single amino acid could not recognize a codon by itself. Consequently, an amino acid is attached to a specific tR ...
... The fidelity of protein synthesis requires the accurate recognition of three-base codons on messenger RNA. Recall that the genetic code relates each amino acid to a three-letter codon. A single amino acid could not recognize a codon by itself. Consequently, an amino acid is attached to a specific tR ...
... Choice A; Since the Km increased at lower pH, the binding is getting weaker due to protonation of a group on either the substrate or the enzyme. The substrate contains two acidic sidechains, and when these get protonated they will become neutral. Therefore the enzyme probably has positively charged ...
I Have a Dream: Organic Movements Include Gene Manipulation to
... the genetic material of plants, animals, microorganisms, cells and other biological units are altered in ways or with results that could not be obtained by methods of natural mating and reproduction or natural recombination”. Therefore, approaches of NBPTs that manipulate the genome in such a way th ...
... the genetic material of plants, animals, microorganisms, cells and other biological units are altered in ways or with results that could not be obtained by methods of natural mating and reproduction or natural recombination”. Therefore, approaches of NBPTs that manipulate the genome in such a way th ...
Regulation of transcript encoding the 43K
... Similarly, antibodies against the 43K protein have been used to detect the 43K protein at synapses, but these immunochemical methods are not sufficiently sensitive to determine whether the 43K protein is present at nonsynaptic regions of denervated and developing muscle (Froehner et al. 1981; Burden ...
... Similarly, antibodies against the 43K protein have been used to detect the 43K protein at synapses, but these immunochemical methods are not sufficiently sensitive to determine whether the 43K protein is present at nonsynaptic regions of denervated and developing muscle (Froehner et al. 1981; Burden ...
The complete nucleotide sequence of the RNA coding for the
... residue (2, 3 i The VPg protein is not required for translation and, by correlation with poliovirus is probably involved in replication (A, 5). The RNA of FMDV, like other picornaviruses, appears to have a long 5' untranslated leader sequence. Removal of the poly C tract of FMDV and all nucleotides ...
... residue (2, 3 i The VPg protein is not required for translation and, by correlation with poliovirus is probably involved in replication (A, 5). The RNA of FMDV, like other picornaviruses, appears to have a long 5' untranslated leader sequence. Removal of the poly C tract of FMDV and all nucleotides ...
types of gel - WordPress.com
... Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resu ...
... Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resu ...
Salinity Response in Chloroplasts: Insights from Gene
... Chloroplasts are unique organelles due to their highly oxidizing metabolic activity and increased rate of electron flow, being especially prone to generating ROS, such as superoxide anion (O2 − ), hydrogen peroxide (H2 O2 ), singlet oxygen (1 O2 ), and hydroxyl radical (OH•). The presence of ROS pro ...
... Chloroplasts are unique organelles due to their highly oxidizing metabolic activity and increased rate of electron flow, being especially prone to generating ROS, such as superoxide anion (O2 − ), hydrogen peroxide (H2 O2 ), singlet oxygen (1 O2 ), and hydroxyl radical (OH•). The presence of ROS pro ...
CitA (citrate) and DcuS (C 4 -dicarboxylate) sensor kinases in
... by citAGt and by full length citBEc on plasmid, and both genes were under the control of ...
... by citAGt and by full length citBEc on plasmid, and both genes were under the control of ...
Histone Deacetylase 4 Antibody
... Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past se ...
... Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past se ...
Annotation guidelines - Systems Biology and Bioinformatics
... (b) A Zn2+ ion is bound within each active site,… (c) It is suggested that anticapsin behaves as a glutamine analogue… As shown in (a)-(c), ion and analogue are counted as a part of Metabolite entity. 3. Co-factor is annotated as in the Metabolite entity. (d) Either NADP+ or NAD+ function as cofacto ...
... (b) A Zn2+ ion is bound within each active site,… (c) It is suggested that anticapsin behaves as a glutamine analogue… As shown in (a)-(c), ion and analogue are counted as a part of Metabolite entity. 3. Co-factor is annotated as in the Metabolite entity. (d) Either NADP+ or NAD+ function as cofacto ...
Over-expression of UDP-glucose pyrophosphorylase in hybrid
... monitor endogenous and environmental cues, and use this information to regulate resource allocation. Ultimately, these responses are controlled by gene expression patterns, resulting in the synthesis of a variety of metabolites, including carbohydrates, which can accumulate, participate in metabolit ...
... monitor endogenous and environmental cues, and use this information to regulate resource allocation. Ultimately, these responses are controlled by gene expression patterns, resulting in the synthesis of a variety of metabolites, including carbohydrates, which can accumulate, participate in metabolit ...
The relative rates of synthesis of DNA, sRNA and rRNA in the
... H. R. WOODLAND & J. B. GURDON false comparison of RNA synthesis in this region compared with the endoderm. This effect will be minimal if the acid-soluble pool is not greatly depleted of labelled precursor during the incubation period. In our experiments we have found that never more than half of th ...
... H. R. WOODLAND & J. B. GURDON false comparison of RNA synthesis in this region compared with the endoderm. This effect will be minimal if the acid-soluble pool is not greatly depleted of labelled precursor during the incubation period. In our experiments we have found that never more than half of th ...
Involvement of Endogenous Retroviruses in Prion Diseases
... As mentioned above, endogenous retroviral elements have been postulated to be involved in various complex diseases including neurological disorders; interestingly, the activation of ERVs has also been observed in prion diseases. First, infection of a senescence-accelerated mouse strain (SAMP8) that ...
... As mentioned above, endogenous retroviral elements have been postulated to be involved in various complex diseases including neurological disorders; interestingly, the activation of ERVs has also been observed in prion diseases. First, infection of a senescence-accelerated mouse strain (SAMP8) that ...
Effect of RNAi down-regulation of three lysine-deficient
... content of the essential amino acids lysine and methionine. The chapter concludes by formulating a genetic engineering strategy that aims at improving the seed lysine content. The first technical effort towards achieving the main aims is covered in Chapter 2. This involves in vitro screening of five ...
... content of the essential amino acids lysine and methionine. The chapter concludes by formulating a genetic engineering strategy that aims at improving the seed lysine content. The first technical effort towards achieving the main aims is covered in Chapter 2. This involves in vitro screening of five ...
Structural Characterization of Humanized Nanobodies with
... on Linker 1 connecting Blocks I and II within the CyaA-RTX subdomain that could be a potential neutralizing epitope of CyaA-protective antigen. 2. Results and Discussion 2.1. Isolated CyaA-Hly-Specific Nanobodies with Different CDR-3 Loops Previously, we have succeeded in producing phage-display nan ...
... on Linker 1 connecting Blocks I and II within the CyaA-RTX subdomain that could be a potential neutralizing epitope of CyaA-protective antigen. 2. Results and Discussion 2.1. Isolated CyaA-Hly-Specific Nanobodies with Different CDR-3 Loops Previously, we have succeeded in producing phage-display nan ...
Definition
... other new observations like cheese, curd, etc. Certainly, cheese can be considered as one of the first direct products (or by-product) of biotechnology, because it was prepared by adding rennet (an enzyme found in the stomach of calves) to sour milk, which is possible only by exposing milk to microb ...
... other new observations like cheese, curd, etc. Certainly, cheese can be considered as one of the first direct products (or by-product) of biotechnology, because it was prepared by adding rennet (an enzyme found in the stomach of calves) to sour milk, which is possible only by exposing milk to microb ...
Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... was performed on a Pharmacia Gene Assembler Plus on 0.2pmol capacity columns as recommended by the manufacturer. The oligonucleotides were purified after deprotection by gel filtration on Sephadex G-25 (NAP 5; Pharmacia). The following degenerated oligonucleotides were used in PCR amplifications (nu ...
... was performed on a Pharmacia Gene Assembler Plus on 0.2pmol capacity columns as recommended by the manufacturer. The oligonucleotides were purified after deprotection by gel filtration on Sephadex G-25 (NAP 5; Pharmacia). The following degenerated oligonucleotides were used in PCR amplifications (nu ...
Plant/Fungi Total RNA Purification 96-Well Kit
... Norgen’s Plant/Fungi Total RNA Purification 96-Well Kit provides a rapid method for the highthroughput isolation of total RNA from plants and fungi. The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the ...
... Norgen’s Plant/Fungi Total RNA Purification 96-Well Kit provides a rapid method for the highthroughput isolation of total RNA from plants and fungi. The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the ...
Fast and simple purification of GST fusion proteins using prepacked
... May vary if used in other columns) ...
... May vary if used in other columns) ...
Document
... Genotype - Phenotype Studies You have candidate gene/region/pathway of interest and samples ready to study: What SNPs are available? How do I find the common SNPs? What is the validation/quality of the SNPs? Are these SNPs informative in my population/samples? What can I download information? How d ...
... Genotype - Phenotype Studies You have candidate gene/region/pathway of interest and samples ready to study: What SNPs are available? How do I find the common SNPs? What is the validation/quality of the SNPs? Are these SNPs informative in my population/samples? What can I download information? How d ...