Molecular Interaction between COP1 and HY5
... AD-COP1 (data not shown). Together, our data demonstrate that the N-terminal 77 amino acids of HY5 are both necessary and sufficient for mediating direct interaction with COP1. Hence, HY5 consists of at least two distinct functional modules, one for the presumed dimerization and DNA-binding activity ...
... AD-COP1 (data not shown). Together, our data demonstrate that the N-terminal 77 amino acids of HY5 are both necessary and sufficient for mediating direct interaction with COP1. Hence, HY5 consists of at least two distinct functional modules, one for the presumed dimerization and DNA-binding activity ...
Genetic Engineering for Improved Xylose Fermentation by Yeasts
... heterologous expression of an eukaryotic enzyme could facilitate genetic engineering of xylose metabolism in S. cerevisiae. Unfortunately, they found that R. toruloides uses an oxidoreductase system like other eukaryotes. Other approaches, however, have been more successful. Metabolic engineering [3 ...
... heterologous expression of an eukaryotic enzyme could facilitate genetic engineering of xylose metabolism in S. cerevisiae. Unfortunately, they found that R. toruloides uses an oxidoreductase system like other eukaryotes. Other approaches, however, have been more successful. Metabolic engineering [3 ...
mTORC1 Activates SREBP-1c and Uncouples Lipogenesis From Gluconeogenesis Please share
... tightly regulated by mTORC1 under physiological conditions. The identification of mTORC1 as the bifurcation point that separates hepatic lipogenesis from gluconeogenesis represents an important advance in our understanding of the molecular mechanisms linking hepatic insulin resistance to hyperglycemi ...
... tightly regulated by mTORC1 under physiological conditions. The identification of mTORC1 as the bifurcation point that separates hepatic lipogenesis from gluconeogenesis represents an important advance in our understanding of the molecular mechanisms linking hepatic insulin resistance to hyperglycemi ...
Applied and Environmental Microbiology
... The PCR-amplified genes of the isolates were subjected to further characterization by restriction enzyme digestion (Table 1). The predicted restriction sites for the gene of B. cereus DSM 3101T were confirmed. The specific restriction sites for EcoRI, HindIII, and PstI were present in most of the ba ...
... The PCR-amplified genes of the isolates were subjected to further characterization by restriction enzyme digestion (Table 1). The predicted restriction sites for the gene of B. cereus DSM 3101T were confirmed. The specific restriction sites for EcoRI, HindIII, and PstI were present in most of the ba ...
Internal expression of Yarrowia NDH2
... spores carrying these deletion alleles, either by random spore analysis or by ascus dissection. As we also failed to introduce the deletion alleles directly into haploid yeast strains, it seemed that complex I was essential for vegetative growth. However, we could not exclude at this point that comp ...
... spores carrying these deletion alleles, either by random spore analysis or by ascus dissection. As we also failed to introduce the deletion alleles directly into haploid yeast strains, it seemed that complex I was essential for vegetative growth. However, we could not exclude at this point that comp ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... cows, we cloned and sequenced the full-length cDNA of bovine SGLT1 and SGLT5. Open reading frame analysis predicted that bovine SGLT1 is composed of 664 amino acids with a molecular weight of approximately 73 kDa, and SGLT5 is composed of 597 amino acids with a molecular weight of approximately 65 k ...
... cows, we cloned and sequenced the full-length cDNA of bovine SGLT1 and SGLT5. Open reading frame analysis predicted that bovine SGLT1 is composed of 664 amino acids with a molecular weight of approximately 73 kDa, and SGLT5 is composed of 597 amino acids with a molecular weight of approximately 65 k ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... properties. Moreover, applying force can be used to alter the energy landscape in which a DNA-protein complex operates, yielding unique insights into the thermodynamics and kinetics of DNA-protein interactions. Three distinct force-based techniques have been applied to DNA-protein interactions [132] ...
... properties. Moreover, applying force can be used to alter the energy landscape in which a DNA-protein complex operates, yielding unique insights into the thermodynamics and kinetics of DNA-protein interactions. Three distinct force-based techniques have been applied to DNA-protein interactions [132] ...
attached paper highlights
... PGC-1a in myotubes strongly induces the expression of downstream transcription factors such as NRFs and Tfam.17 Unlike NRFs or Tfam, however, PGC-1a levels correlate with cardiac and skeletal muscle oxidative capacity, suggesting that it plays a major role in setting mitochondrial content.18 PGC-1a ...
... PGC-1a in myotubes strongly induces the expression of downstream transcription factors such as NRFs and Tfam.17 Unlike NRFs or Tfam, however, PGC-1a levels correlate with cardiac and skeletal muscle oxidative capacity, suggesting that it plays a major role in setting mitochondrial content.18 PGC-1a ...
Molecular Characterisation of the 76 kDa Iron
... Max-Volmer-Institut fur Biophysikalische Chemie und Biochemie, Technische Universitdt Berlin, Strasse des 17. Juni 135, D-10623 Berlin, Germany ...
... Max-Volmer-Institut fur Biophysikalische Chemie und Biochemie, Technische Universitdt Berlin, Strasse des 17. Juni 135, D-10623 Berlin, Germany ...
NTPase/helicase of Flaviviridae: inhibitors and inhibition of the
... RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral si ...
... RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral si ...
OPTIMIZER: a web server for optimizing the codon usage of DNA
... highly expressed genes usually use the minimal subset of codons that are recognized by the most abundant tRNA species, their ENc values are expected to be low. Figure 1 shows some of the outputs provided by the optimization of a DNA sequence: for example, the query and optimized sequences and an ali ...
... highly expressed genes usually use the minimal subset of codons that are recognized by the most abundant tRNA species, their ENc values are expected to be low. Figure 1 shows some of the outputs provided by the optimization of a DNA sequence: for example, the query and optimized sequences and an ali ...
Chromatin Association of Gcn4 Is Limited by Post
... levels, Cdk8 (previously known as Srb10) phosphorylates Gcn4, also causing its ubiquitinmediated proteolysis (Chi et al. 2001). However, Cdk8 is a component of the Mediator complex which is recruited to promoters by Gcn4, suggesting that this kinase specifically targets promoter-bound Gcn4 (Rawal et ...
... levels, Cdk8 (previously known as Srb10) phosphorylates Gcn4, also causing its ubiquitinmediated proteolysis (Chi et al. 2001). However, Cdk8 is a component of the Mediator complex which is recruited to promoters by Gcn4, suggesting that this kinase specifically targets promoter-bound Gcn4 (Rawal et ...
The Art of Multiple Sequence Alignment in R
... on sequences of the same kind. In this case, multiple alignment works by aligning two sequences, merging with another sequence, merging with another set of sequences, and so-forth until all the sequences are aligned. This process is iterated to further refine the alignment. There are other functions ...
... on sequences of the same kind. In this case, multiple alignment works by aligning two sequences, merging with another sequence, merging with another set of sequences, and so-forth until all the sequences are aligned. This process is iterated to further refine the alignment. There are other functions ...
Interconnections Between RNA-Processing Pathways Revealed by
... genes were identified whose deletions resulted in defects in pre-mRNA splicing; enriched among ...
... genes were identified whose deletions resulted in defects in pre-mRNA splicing; enriched among ...
dbPSP: a curated database for protein phosphorylation sites in
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
Characterization of the unique intron
... suggesting that it was derived from the LHCPII transcript encoded by GC7. The five exons identified in GC7 can be combined into an open reading frame encoding the 33 kDa N-terminal portion of pLHCPII consisting of a 141 amino acid N-terminal extension (11) linked to an LHCPII unit, N1PEP (Fig. 2). T ...
... suggesting that it was derived from the LHCPII transcript encoded by GC7. The five exons identified in GC7 can be combined into an open reading frame encoding the 33 kDa N-terminal portion of pLHCPII consisting of a 141 amino acid N-terminal extension (11) linked to an LHCPII unit, N1PEP (Fig. 2). T ...
... showed significant homology. The molecular mass of streptavidin was previously reported to be 60 kDa for the product isolated as described above by an initial ammonium sulfate precipitation (many commercially available products are prepared with such methods). From the gene sequence, a mass of 66 kD ...
Nucleosides, Nucleotides,Nucleic Acids
... Structure of DNA James D. Watson and Francis H. C. Crick proposed a structure for DNA in 1953. Watson and Crick's structure was based on: •Chargaff's observations •X-ray crystallographic data of Maurice Wilkins and Rosalind Franklin ...
... Structure of DNA James D. Watson and Francis H. C. Crick proposed a structure for DNA in 1953. Watson and Crick's structure was based on: •Chargaff's observations •X-ray crystallographic data of Maurice Wilkins and Rosalind Franklin ...
The SRA gen - uri=dna.kdna.ucla
... Antigenic Variation-Linked Switching from Sensitivity to Resistance in ETat 1 In the ETat 1 strain of T.b. rhodesiense, the selection of R clones systematically resulted in the expression of the ETat 1.10 VSG, and counter-selection was necessary in order to generate R clones expressing different VSG ...
... Antigenic Variation-Linked Switching from Sensitivity to Resistance in ETat 1 In the ETat 1 strain of T.b. rhodesiense, the selection of R clones systematically resulted in the expression of the ETat 1.10 VSG, and counter-selection was necessary in order to generate R clones expressing different VSG ...
FEBS Letters
... the cyanobacterium Synechocystis sp. (strain PCC 6803). Especially the putative NADPH binding motif `LGSTGSIG' [7] was completely conserved at position 7 of the deduced amino acid sequence. In contrast to the situation in S. leopoliensis, where the gene dxr is part of an operon, in Synechocystis sp. ...
... the cyanobacterium Synechocystis sp. (strain PCC 6803). Especially the putative NADPH binding motif `LGSTGSIG' [7] was completely conserved at position 7 of the deduced amino acid sequence. In contrast to the situation in S. leopoliensis, where the gene dxr is part of an operon, in Synechocystis sp. ...
A Survey of Flexible Protein Binding Mechanisms and their
... Many cellular functions rely on interactions between protein pairs and higher oligomers. We have recently shown that binding mechanisms are robust and owing to the minimal frustration principle, just as for protein folding, are governed primarily by the protein’s native topology, which is characteri ...
... Many cellular functions rely on interactions between protein pairs and higher oligomers. We have recently shown that binding mechanisms are robust and owing to the minimal frustration principle, just as for protein folding, are governed primarily by the protein’s native topology, which is characteri ...
Drought response in the spikes of barley: gene expression in the
... Differential gene expression among drought-stressed spike organs was analyzed using a mixed linear model analysis (Nettleton 2006). The log2 expression values were the response variables, drought stress and organ type were fixed treatment effects, and date of sample collection (block) and residuals ...
... Differential gene expression among drought-stressed spike organs was analyzed using a mixed linear model analysis (Nettleton 2006). The log2 expression values were the response variables, drought stress and organ type were fixed treatment effects, and date of sample collection (block) and residuals ...
Comparison of conserved structural and regulatory domains within
... morphological and physiological diversity is mirrored by extensive genetic variability, cyanobacterial genomes ranging in mean DNA base composition from 32 to 71 mol % GjC (Herdman et al., 1979b ; M. Herdman & R. Rippka, unpublished) and in complexity from 2n0 to 13n2 Mbp (Herdman et al., 1979a ; M. ...
... morphological and physiological diversity is mirrored by extensive genetic variability, cyanobacterial genomes ranging in mean DNA base composition from 32 to 71 mol % GjC (Herdman et al., 1979b ; M. Herdman & R. Rippka, unpublished) and in complexity from 2n0 to 13n2 Mbp (Herdman et al., 1979a ; M. ...