Threading-based Protein Structure Prediction
... – Eukaryotes (organisms with single or multiple cells. their cells have ...
... – Eukaryotes (organisms with single or multiple cells. their cells have ...
TAP Express® Kit
... This step generates the TAP Primary® fragment, a DNA fragment that contains the gene-ofinterest with the added 5’- and 3’-TAP Ends sequences. These 5’- and 3’-TAP Ends sequences are necessary for adding the TAP Express® promoter and terminator fragments in the second PCR step. The conditions recomme ...
... This step generates the TAP Primary® fragment, a DNA fragment that contains the gene-ofinterest with the added 5’- and 3’-TAP Ends sequences. These 5’- and 3’-TAP Ends sequences are necessary for adding the TAP Express® promoter and terminator fragments in the second PCR step. The conditions recomme ...
Nucleic Acids Research
... most of these have been of the G-+T type when comparing the pTi T37 to the pTi Ach5 sequence. Without quantitative comparison of transcription levels from the two tmr loci, it is not possible to assess the overall effects of these base changes on relative promoter strength. Heidekamp et al. found tw ...
... most of these have been of the G-+T type when comparing the pTi T37 to the pTi Ach5 sequence. Without quantitative comparison of transcription levels from the two tmr loci, it is not possible to assess the overall effects of these base changes on relative promoter strength. Heidekamp et al. found tw ...
proposal-aug25
... Requested: $137775 short conserved segments that fall within disordered regions, but do not match any known protein domains. To estimate the expected number of false positives identified in the cluster analysis we will repeat the clustering on the motifs identified in simulated disordered proteins. ...
... Requested: $137775 short conserved segments that fall within disordered regions, but do not match any known protein domains. To estimate the expected number of false positives identified in the cluster analysis we will repeat the clustering on the motifs identified in simulated disordered proteins. ...
Focus Issue on Plastid Biology Update Novel
... activase are controlled by thioredoxin. Trx f donates electrons to target proteins that have a broad range of redox midpoint potentials Em (Hutchinson et al. 2000). Differential inactivation of target proteins e.g. in the Calvin cycle, is unrelated to the value of Em but highly relevant for photoin ...
... activase are controlled by thioredoxin. Trx f donates electrons to target proteins that have a broad range of redox midpoint potentials Em (Hutchinson et al. 2000). Differential inactivation of target proteins e.g. in the Calvin cycle, is unrelated to the value of Em but highly relevant for photoin ...
Chapter 14 Preparing Semisynthetic and Fully Synthetic Histones
... 1. Introduction Nucleosomes, the fundamental unit of chromatin, are protein–DNA complexes that package and organize DNA in the nucleus of the cell (1). The core nucleosome particle contains two copies each of four histone proteins: H3, H4, H2A, and H2B. Each of these histone proteins has multiple si ...
... 1. Introduction Nucleosomes, the fundamental unit of chromatin, are protein–DNA complexes that package and organize DNA in the nucleus of the cell (1). The core nucleosome particle contains two copies each of four histone proteins: H3, H4, H2A, and H2B. Each of these histone proteins has multiple si ...
- Wiley Online Library
... either simple or complex organic media. These are the obligate autotrophs and type I methanotrophs, whose one-carbon (C1 )-compound-dependent metabolism requires the fixation of carbon dioxide using energy from inorganic oxidations or light, or uses methane (or in some cases methanol) as both energy ...
... either simple or complex organic media. These are the obligate autotrophs and type I methanotrophs, whose one-carbon (C1 )-compound-dependent metabolism requires the fixation of carbon dioxide using energy from inorganic oxidations or light, or uses methane (or in some cases methanol) as both energy ...
E. coli
... glucosamine-6-phosphate, CTP, and AMP Each of the inhibitors provides only partial inhibition, complete inhibition requires all of the inhibitors. Kinetic studies suggested that none of the inhibitors was competitive ...
... glucosamine-6-phosphate, CTP, and AMP Each of the inhibitors provides only partial inhibition, complete inhibition requires all of the inhibitors. Kinetic studies suggested that none of the inhibitors was competitive ...
Pseudomon-1 motif
... Naming candidate RNA motifs ....................................................................................... 6 Experimental analysis of SAM binding by SAM/SAH-binding RNAs.............................. 6 Additional discussion of candidate RNA motifs ........................................... ...
... Naming candidate RNA motifs ....................................................................................... 6 Experimental analysis of SAM binding by SAM/SAH-binding RNAs.............................. 6 Additional discussion of candidate RNA motifs ........................................... ...
... it is in the hydrophobic core. Since this Thr would form an H-bond to water in the unfolded state, it will cost about 20 kJ/mole of instability since a hydrogen bond to water is broken during folding, but not reformed. (6 pts) Additional factors (1 pt for either, unless the main reason was missed an ...
Dynamic modification of the ETS transcription factor PEA3 by
... expressed in mouse embryonic carcinoma F9 cells (29) and was precipitated using and anti-PEA3 antibody and acetylation detected using an anti-acetyl lysine antibody. Two bands corresponding to PEA3 were identified, and acetylation of the upper band was detected (Figure 1A). We also asked whether acet ...
... expressed in mouse embryonic carcinoma F9 cells (29) and was precipitated using and anti-PEA3 antibody and acetylation detected using an anti-acetyl lysine antibody. Two bands corresponding to PEA3 were identified, and acetylation of the upper band was detected (Figure 1A). We also asked whether acet ...
Arctigenin Efficiently Enhanced Sedentary Mice Treadmill
... improvement in obesity. AMP-activated protein kinase (AMPK) as an energy sensor plays pivotal roles in the regulation of energy homeostasis, and its activation could improve glucose uptake, promote mitochondrial biogenesis and increase glycolysis. Recent research has even suggested that AMPK activat ...
... improvement in obesity. AMP-activated protein kinase (AMPK) as an energy sensor plays pivotal roles in the regulation of energy homeostasis, and its activation could improve glucose uptake, promote mitochondrial biogenesis and increase glycolysis. Recent research has even suggested that AMPK activat ...
Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)
... roles in other major parts of the carbon cycle such as photosynthesis, no genome-level studies have been published on the biology of methanotrophs. We report the first complete genome sequence to our knowledge from an obligate methanotroph, Methylococcus capsulatus (Bath), obtained by the shotgun se ...
... roles in other major parts of the carbon cycle such as photosynthesis, no genome-level studies have been published on the biology of methanotrophs. We report the first complete genome sequence to our knowledge from an obligate methanotroph, Methylococcus capsulatus (Bath), obtained by the shotgun se ...
Large-scale Analysis of Pseudogenes in the Human Genome
... other eukaryotic genomes. Identification of these pseudogenes can improve the accuracy of gene annotation. It also offers new insight on the evolution history of human genes and the stability of genome as a whole. ...
... other eukaryotic genomes. Identification of these pseudogenes can improve the accuracy of gene annotation. It also offers new insight on the evolution history of human genes and the stability of genome as a whole. ...
Practical Aspects of Estimating Energy Components in
... transcription from RNA polymerase II promoter GO: 0006367 transcription initiation from RNA polymerase II promoter GO: 0006368 transcription elongation from RNA polymerase II promoter GO: 0006370 7-methylguanosine mRNA capping GO: 0006468 protein phosphorylation GO: 0010467 gene expression GO: 00160 ...
... transcription from RNA polymerase II promoter GO: 0006367 transcription initiation from RNA polymerase II promoter GO: 0006368 transcription elongation from RNA polymerase II promoter GO: 0006370 7-methylguanosine mRNA capping GO: 0006468 protein phosphorylation GO: 0010467 gene expression GO: 00160 ...
Biochemistry 499
... Abnormal phosphorylation has been known as a cause or consequence of many human diseases, such as cancer. This stresses the importance of regulation of the enzymes involved in reversible phosphorylation, especially the protein phosphatases. There are relatively few Ser/Thr phosphatases in the cell a ...
... Abnormal phosphorylation has been known as a cause or consequence of many human diseases, such as cancer. This stresses the importance of regulation of the enzymes involved in reversible phosphorylation, especially the protein phosphatases. There are relatively few Ser/Thr phosphatases in the cell a ...
ALLOMETRIC SCALING IN CENTRARCHID FISH Enzyme Levels in Muscles
... been investigated since the 1800’s, Rubner (1883) found metabolic rate to scale with surface area in dogs (see Hulbert and Else 2004). It was not until the twentieth century when common themes in scaling relationships of whole animal metabolic rates emerged. In 1932, Kleiber analyzed the metabolic ...
... been investigated since the 1800’s, Rubner (1883) found metabolic rate to scale with surface area in dogs (see Hulbert and Else 2004). It was not until the twentieth century when common themes in scaling relationships of whole animal metabolic rates emerged. In 1932, Kleiber analyzed the metabolic ...
NIH Public Access
... chromosome that has a GC content of 56 % (Supplementary Figure S1). Base pair one of the chromosome was assigned within the putative origin of replication. The genome contains 51 tRNAs, 3 rRNAs (5S, 16S, and 23S), and 38 other RNAs predicted by the Rfam. Analysis of 16S rRNA sequences confirmed that ...
... chromosome that has a GC content of 56 % (Supplementary Figure S1). Base pair one of the chromosome was assigned within the putative origin of replication. The genome contains 51 tRNAs, 3 rRNAs (5S, 16S, and 23S), and 38 other RNAs predicted by the Rfam. Analysis of 16S rRNA sequences confirmed that ...
cloning of gs1 gene encodes glutamine synthetase 1 and
... plays an essential role in the metabolism of nitrogen. In vascular plants, GS is devided into two groups: cytosol - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown that GS1 gene was used widely to generate transgenic plants. Effects on the growth characteristics of GS1 ...
... plays an essential role in the metabolism of nitrogen. In vascular plants, GS is devided into two groups: cytosol - located isoform (GS1) and plastid – located isoform (GS2). Surveys have shown that GS1 gene was used widely to generate transgenic plants. Effects on the growth characteristics of GS1 ...
- Wiley Online Library
... be provided upon request) were cloned into pCZ1016, a derivative of pFAJ1700 containing the Ptac promoter, multiple cloning sites and the T7 terminator from pSC150 (Dombrecht et al., 2001; Cunnac, 2004). To perform chromosomal complementations, PCR amplicons were cloned into pCZ1034, a derivative of ...
... be provided upon request) were cloned into pCZ1016, a derivative of pFAJ1700 containing the Ptac promoter, multiple cloning sites and the T7 terminator from pSC150 (Dombrecht et al., 2001; Cunnac, 2004). To perform chromosomal complementations, PCR amplicons were cloned into pCZ1034, a derivative of ...
Transcriptome analysis of the two unrelated fungal
... and production strains were only performed for P. chrysogenum [9, 10]. This work detected mainly single nucleotide polymorphisms (SNPs), which let the authors conclude that expressional changes are largely responsible for observed enhanced penicillin titers. In line of this conclusion, transcription ...
... and production strains were only performed for P. chrysogenum [9, 10]. This work detected mainly single nucleotide polymorphisms (SNPs), which let the authors conclude that expressional changes are largely responsible for observed enhanced penicillin titers. In line of this conclusion, transcription ...
Journal of Biotechnology
... package Mauve (Darling et al., 2004) was used to compare the genomes of B. amyloliquefaciens FZB42, B. subtilis 168, B. licheniformis and B. pumilus. The four species belong to a closely related taxonomic unit vernacularly called the B. subtilis group (Fritze, 2004). A core genome formed by the four ...
... package Mauve (Darling et al., 2004) was used to compare the genomes of B. amyloliquefaciens FZB42, B. subtilis 168, B. licheniformis and B. pumilus. The four species belong to a closely related taxonomic unit vernacularly called the B. subtilis group (Fritze, 2004). A core genome formed by the four ...
Bio 226: Cell and Molecular Biology
... • Lack NLS, nuclear import requires FHY1 and FHL • Slow responses are due to changes in gene expression ...
... • Lack NLS, nuclear import requires FHY1 and FHL • Slow responses are due to changes in gene expression ...
Comparative transcriptomics reveals key gene expression
... Mycobacterium bovis genome sequences revealed no unique genes in the bovine pathogen per se, indicating that differences in gene expression may play a significant role in host predilection. To define the key gene expression differences between M. tuberculosis and M. bovis we have performed transcrip ...
... Mycobacterium bovis genome sequences revealed no unique genes in the bovine pathogen per se, indicating that differences in gene expression may play a significant role in host predilection. To define the key gene expression differences between M. tuberculosis and M. bovis we have performed transcrip ...
chapter 1: exploring life
... CHAPTER 5: THE STRUCTURE AND FUNCTION OF MACROMOLECULES KEY CONCEPTS: 1.Most macromolecules are polymers, built from monomers. 2.Carbohydrates serve as fuel and building material. 3.Lipids are a diverse group of hydrophobic molecules. 4.Proteins have many structures, resulting in a wide range of fun ...
... CHAPTER 5: THE STRUCTURE AND FUNCTION OF MACROMOLECULES KEY CONCEPTS: 1.Most macromolecules are polymers, built from monomers. 2.Carbohydrates serve as fuel and building material. 3.Lipids are a diverse group of hydrophobic molecules. 4.Proteins have many structures, resulting in a wide range of fun ...