Rhizopus Raw-Starch-Degrading Glucoamylase: Its
... glucoamylase gene was not expressed in yeast cells, we have cloned a glucoamylase gene from a CDNAlibrary prepared from Rhizopus mRNA.Sequence analysis of both glucoamylase genes revealed that the genomic gene contained 4 intervening sequences and the CDNAgene lacked 145 nucleotides corresponding to ...
... glucoamylase gene was not expressed in yeast cells, we have cloned a glucoamylase gene from a CDNAlibrary prepared from Rhizopus mRNA.Sequence analysis of both glucoamylase genes revealed that the genomic gene contained 4 intervening sequences and the CDNAgene lacked 145 nucleotides corresponding to ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... Siddavattam, and Klingmüller 1995), or in CO2-fixation as pyruvate synthase (Chlorobium) (Yoon et al. 1999). On the other hand, among eukaryotes, PFO is only known to function in oxidative pyruvate decarboxylation in amitochondriate protists, where the electrons are donated to Fdox in hydrogenosome ...
... Siddavattam, and Klingmüller 1995), or in CO2-fixation as pyruvate synthase (Chlorobium) (Yoon et al. 1999). On the other hand, among eukaryotes, PFO is only known to function in oxidative pyruvate decarboxylation in amitochondriate protists, where the electrons are donated to Fdox in hydrogenosome ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... transmitted by arthropod vectors to man and animals, in Which they may cause serious disease. The pathogens caus ...
... transmitted by arthropod vectors to man and animals, in Which they may cause serious disease. The pathogens caus ...
The mitochondrial carnitine/acylcarnitine carrier: Function
... cDNAs contain an open reading frame of 903 bp coding for a protein of 301 amino acids. Based on this information, CAC orthologs from different species have been predicted and in some cases validated (Fig. 3). CAC belongs to the mitochondrial carrier protein family (Palmieri, 2004; Picault et al., 20 ...
... cDNAs contain an open reading frame of 903 bp coding for a protein of 301 amino acids. Based on this information, CAC orthologs from different species have been predicted and in some cases validated (Fig. 3). CAC belongs to the mitochondrial carrier protein family (Palmieri, 2004; Picault et al., 20 ...
Identification and characterization of novel interaction
... Results .......................................................................................................................................... 41 ...
... Results .......................................................................................................................................... 41 ...
attachment of amino acids to tRNA
... exchange GDP for GTP prior to participating in a new round of elongation. 1. EF-G-GDP: GDP has a lower affinity, and GDP is released after GTP hydrolysis. The free EF-G rapidly binds a new GTP. 2. EF-Tu-GDP requires a GTP exchange factor EF-Ts to displace GDP and recruit GTP to EF-Tu. ...
... exchange GDP for GTP prior to participating in a new round of elongation. 1. EF-G-GDP: GDP has a lower affinity, and GDP is released after GTP hydrolysis. The free EF-G rapidly binds a new GTP. 2. EF-Tu-GDP requires a GTP exchange factor EF-Ts to displace GDP and recruit GTP to EF-Tu. ...
Minimal metabolic pathway structure is consistent with
... same transcription factor (TF). We compared MinSpan pathways to PPI and genetic interactions in S. cerevisiae and the TRN of E. coli as the datasets are most complete for those particular organisms (Fig 2C). By testing for significant Spearman correlation coefficients of cooccurrence or co-absence o ...
... same transcription factor (TF). We compared MinSpan pathways to PPI and genetic interactions in S. cerevisiae and the TRN of E. coli as the datasets are most complete for those particular organisms (Fig 2C). By testing for significant Spearman correlation coefficients of cooccurrence or co-absence o ...
Iron mediated methylthiolation of tRNA as a regulator of operon
... extract from the bacteria grown in broth containing ovotransferrin, however, incorporated nearly twice this amount, 70 pp moles per 150 pi volume. The results were consistent in that extracts from the ovotransferrin grown cells were always slightly more active with MS2 RNA than those from the iron r ...
... extract from the bacteria grown in broth containing ovotransferrin, however, incorporated nearly twice this amount, 70 pp moles per 150 pi volume. The results were consistent in that extracts from the ovotransferrin grown cells were always slightly more active with MS2 RNA than those from the iron r ...
Gene Section MUC4 (mucin 4, cell surface associated) in Oncology and Haematology
... Many studies has been conducted on human pancreatic or other cancer cell lines in order to elucidate molecular mechanisms responsible for aberrant expression of MUC4 in diseased condition. Initial studies about MUC4 transcriptional regulation showed that Sp1 and Sp3 were important regulators of MUC4 ...
... Many studies has been conducted on human pancreatic or other cancer cell lines in order to elucidate molecular mechanisms responsible for aberrant expression of MUC4 in diseased condition. Initial studies about MUC4 transcriptional regulation showed that Sp1 and Sp3 were important regulators of MUC4 ...
Supplementary materials - Stanford Artificial Intelligence Laboratory
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
DORNRÖSCHEN is a direct target of the auxin response factor
... with an additional 20% of drn drnl seedlings showing postgermination basal domain defects. Genetically, DRN and DRNL are highly redundant during embryonic patterning (Chandler et al., 2007). This conclusion is validated by protein-interaction studies showing that both DRN and DRNL interact with Clas ...
... with an additional 20% of drn drnl seedlings showing postgermination basal domain defects. Genetically, DRN and DRNL are highly redundant during embryonic patterning (Chandler et al., 2007). This conclusion is validated by protein-interaction studies showing that both DRN and DRNL interact with Clas ...
Next Generation Quantitative PCR
... allowing the user to easily incorporate a reliable positive control into ...
... allowing the user to easily incorporate a reliable positive control into ...
The Role of Carbohydrate Response Element Binding Protein in
... the liver. ChREBP target genes are involved in glycolysis (Glut2, liver pyruvate kinase), fructolysis (Glut5, ketohexokinase), and lipogenesis (acetyl CoA carboxylase, fatty acid synthase). ChREBP gene deletion protects against high sucrose diet-induced and leptin-deficient obesity, because Chrebp−/ ...
... the liver. ChREBP target genes are involved in glycolysis (Glut2, liver pyruvate kinase), fructolysis (Glut5, ketohexokinase), and lipogenesis (acetyl CoA carboxylase, fatty acid synthase). ChREBP gene deletion protects against high sucrose diet-induced and leptin-deficient obesity, because Chrebp−/ ...
biolsci.org - International Journal of Biological Sciences
... target. Additionally, the resolved Dxs crystal structure from E. coli and Deinococcus radiodurans can further benefit the rational design of inhibitors against it. Dxs contains three domains (I, II, and III), each shares homology with the equivalent domains of TK and the E1 subunit of PDH [52]. Alth ...
... target. Additionally, the resolved Dxs crystal structure from E. coli and Deinococcus radiodurans can further benefit the rational design of inhibitors against it. Dxs contains three domains (I, II, and III), each shares homology with the equivalent domains of TK and the E1 subunit of PDH [52]. Alth ...
Get PDF - Wiley Online Library
... cell is one-fifth of that of the original eIF4GI, for unknown reasons. In addition, the extended eIF4GI could not be extracted with the buffer used (see Materials and methods) as efficiently as the original eIF4GI, probably because the extended N-terminal region of eIF4GI is rich in hydrophobic amin ...
... cell is one-fifth of that of the original eIF4GI, for unknown reasons. In addition, the extended eIF4GI could not be extracted with the buffer used (see Materials and methods) as efficiently as the original eIF4GI, probably because the extended N-terminal region of eIF4GI is rich in hydrophobic amin ...
Polymerase Chain Reaction In Ophthalmology
... o PCR can detect DNA of dead colonising normal flora of the conjunctiva. However these problems can be to some extent overcome with the advent of newer techniques such as real time PCR. • Again high specificity can give rise to false-negative results, if the target DNA location of the pathogen is pl ...
... o PCR can detect DNA of dead colonising normal flora of the conjunctiva. However these problems can be to some extent overcome with the advent of newer techniques such as real time PCR. • Again high specificity can give rise to false-negative results, if the target DNA location of the pathogen is pl ...
Lecture 1 - "Hudel" Luecke
... two types of secondary structures: alpha-helix and beta-pleated sheet. An alpha-helix is a tight helix formed out of the polypeptide chain. The polypeptide main chain makes up the central structure, and the side chains extend out and away from the helix. The CO group of one amino acid (n) is hydroge ...
... two types of secondary structures: alpha-helix and beta-pleated sheet. An alpha-helix is a tight helix formed out of the polypeptide chain. The polypeptide main chain makes up the central structure, and the side chains extend out and away from the helix. The CO group of one amino acid (n) is hydroge ...
Absence of translationally selected synonymous codon usage
... copy numbers of the genes for those tRNAs, whereas in Hel. pylori only one tRNA (that for Glu) is encoded by more than one gene. Similarly, the anticodons of Hel. pylori tRNAs are not known, but indirect inferences can be made from the sequences of the anticodon sites in the tRNA genes, i.e. prior t ...
... copy numbers of the genes for those tRNAs, whereas in Hel. pylori only one tRNA (that for Glu) is encoded by more than one gene. Similarly, the anticodons of Hel. pylori tRNAs are not known, but indirect inferences can be made from the sequences of the anticodon sites in the tRNA genes, i.e. prior t ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
... that is generated in plastids has a single known fate, the formation of fatty acids (Ohlrogge et al., 1979; Stumpf, 1987; Harwood, 1988); in contrast, cytosolic malonyl-COA is not utilized for de novo fatty acid biosynthesis but for the synthesis of a variety of phytochemicals (Com, 1981; Nikolau, e ...
... that is generated in plastids has a single known fate, the formation of fatty acids (Ohlrogge et al., 1979; Stumpf, 1987; Harwood, 1988); in contrast, cytosolic malonyl-COA is not utilized for de novo fatty acid biosynthesis but for the synthesis of a variety of phytochemicals (Com, 1981; Nikolau, e ...
Nicotinamide adenine dinucleotide, a metabolic regulator of
... pathway is compartmentalised in the nucleus, where NAD can be readily utilised by Sir2p [15]. Consistent with this notion, components of the salvage pathway, such as Npt1p, are localised primarily to the nucleus, whereas components of the de novo pathway reside evenly throughout the cell and have n ...
... pathway is compartmentalised in the nucleus, where NAD can be readily utilised by Sir2p [15]. Consistent with this notion, components of the salvage pathway, such as Npt1p, are localised primarily to the nucleus, whereas components of the de novo pathway reside evenly throughout the cell and have n ...
5 Molecular basis of type-2 diabetes
... have also been implicated in insulin signaling. Unlike the conventional PKCs, aPKCs are not activated by diacylglycerol. However, although aPKCs lack PH domains, phosphatidylinositol 3,4,5-trisphosphate bind to the regulatory domains ...
... have also been implicated in insulin signaling. Unlike the conventional PKCs, aPKCs are not activated by diacylglycerol. However, although aPKCs lack PH domains, phosphatidylinositol 3,4,5-trisphosphate bind to the regulatory domains ...
Supporting document 1 Safety assessment
... DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred during the transformation event. The csr1-2 gene expression cassette in CV127 is identical in sequence to the transforming plasmid DNA except for three point mutations, one o ...
... DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred during the transformation event. The csr1-2 gene expression cassette in CV127 is identical in sequence to the transforming plasmid DNA except for three point mutations, one o ...
488KB - The Doudna Lab - University of California, Berkeley
... produce and help traffic small dsRNAs during RNA interference (RNAi)1. Found in the cytoplasm of nearly all eukaryotic cells, Dicer recognizes the 5¢ and 3¢ helical ends of dsRNA and cleaves a specific distance away to produce 21– to 28–nucleotide (nt) short interfering RNAs (siRNAs)2 or microRNAs3. ...
... produce and help traffic small dsRNAs during RNA interference (RNAi)1. Found in the cytoplasm of nearly all eukaryotic cells, Dicer recognizes the 5¢ and 3¢ helical ends of dsRNA and cleaves a specific distance away to produce 21– to 28–nucleotide (nt) short interfering RNAs (siRNAs)2 or microRNAs3. ...
ppt for
... 2) Olfactory receptors and miRNAs are lost at the same rate with which they are acquired. However, not a single cluster loss was observed for piRNA. ...
... 2) Olfactory receptors and miRNAs are lost at the same rate with which they are acquired. However, not a single cluster loss was observed for piRNA. ...
Coordination of the maize transcriptome by a conserved circadian
... hybridization values, genes on the array exhibiting circadian waveforms were identified by two well-established methods known as COSOPT and HAYSTACK [26,32]. COSOPT is a method that fits gene expression profiles to a series of modified cosine models, with the agreement between the experimental data ...
... hybridization values, genes on the array exhibiting circadian waveforms were identified by two well-established methods known as COSOPT and HAYSTACK [26,32]. COSOPT is a method that fits gene expression profiles to a series of modified cosine models, with the agreement between the experimental data ...