Chlamydia Exploit the Mammalian Tryptophan-Depletion
... accommodate the export of virulence factors that interact with the host biochemical network, and ...
... accommodate the export of virulence factors that interact with the host biochemical network, and ...
The Protein Cevalently Linked to the 5'... of Poliovirus RNA by Victor Robert Ambros
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
Specific and ubiquitous expression of different Zn finger protein
... Many eucaryotic genes involved in the process of differentiation and development are expressed in a temporally and spatially restricted fashion(15). Considering that • regulation of such genes also occur at the transcriptional level,the identification of different transcriptional regulatory factors ...
... Many eucaryotic genes involved in the process of differentiation and development are expressed in a temporally and spatially restricted fashion(15). Considering that • regulation of such genes also occur at the transcriptional level,the identification of different transcriptional regulatory factors ...
Regulation of Starch Synthesis in Cassava Yona Baguma
... Results showed that the transcriptional activity of sbe increased with the developmental states of the storage root. Analysis of sbe diurnal transcript patterns identified the existence of an endogenous semidian oscillator (12 h) in the storage root cells but its nature and function remains unknown. ...
... Results showed that the transcriptional activity of sbe increased with the developmental states of the storage root. Analysis of sbe diurnal transcript patterns identified the existence of an endogenous semidian oscillator (12 h) in the storage root cells but its nature and function remains unknown. ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748
... of SGLT2 in the cDNA library of bovine mammary tissues, indicating its expression in bovine mammary gland. To facilitate study of the mechanism of glucose reabsorption in bovine kidneys in maintenance of glucose homeostasis of lactating cows and the potential role of SGLT2 in the mammary gland, we c ...
... of SGLT2 in the cDNA library of bovine mammary tissues, indicating its expression in bovine mammary gland. To facilitate study of the mechanism of glucose reabsorption in bovine kidneys in maintenance of glucose homeostasis of lactating cows and the potential role of SGLT2 in the mammary gland, we c ...
Potassium starvation responses in yeast highlight novel potassium-related functions
... reason, potassium homeostasis is tightly controlled by several transporters, that allow the cation uptake, intracellular traffic and efflux, and by a set of proteins regulating these processes. In spite of the importance of potassium for yeast physiology, not all relevant functional potassium-relate ...
... reason, potassium homeostasis is tightly controlled by several transporters, that allow the cation uptake, intracellular traffic and efflux, and by a set of proteins regulating these processes. In spite of the importance of potassium for yeast physiology, not all relevant functional potassium-relate ...
Defining the Schistosoma haematobium kinome enables the
... Recent research efforts to identify new molecular targets for chemotherapeutic intervention have focused on protein kinases13,14, because they are involved in signalling cascades of essential regulatory and developmental processes15–17, particular kinase groups have relatively conserved structures18 ...
... Recent research efforts to identify new molecular targets for chemotherapeutic intervention have focused on protein kinases13,14, because they are involved in signalling cascades of essential regulatory and developmental processes15–17, particular kinase groups have relatively conserved structures18 ...
Unveiling the physiological and molecular basis of Mycobacterium
... species, namely M. tuberculosis, the main etiological agent of human tuberculosis (TB), and M. bovis, the causative agent of bovine tuberculosis (bTB), one of the most relevant zoonosis in the world. The complex pathogenesis of MTC bacteria is influenced by several factors, including host and bacter ...
... species, namely M. tuberculosis, the main etiological agent of human tuberculosis (TB), and M. bovis, the causative agent of bovine tuberculosis (bTB), one of the most relevant zoonosis in the world. The complex pathogenesis of MTC bacteria is influenced by several factors, including host and bacter ...
Major players on the microbial stage: why archaea
... used to determine the presence of archaea in environments even when the organisms themselves remained unculturable. In addition to ssrRNA signatures, evidence for an archaeal ‘genomic signature’ has been presented. The archaeal genomic signature presented by Graham et al. (2000) is a set of over 350 ...
... used to determine the presence of archaea in environments even when the organisms themselves remained unculturable. In addition to ssrRNA signatures, evidence for an archaeal ‘genomic signature’ has been presented. The archaeal genomic signature presented by Graham et al. (2000) is a set of over 350 ...
Codon optimization of Col H gene encoding Clostridium
... site obstructs translation initiation. On the other hand, GC-poor mRNAs cannot fold strongly and ...
... site obstructs translation initiation. On the other hand, GC-poor mRNAs cannot fold strongly and ...
Locked Nucleic Acid (LNA™)
... applications in which standard DNA oligonucleotides or RNA riboprobes do not show sufficient affinity or specificity. LNA™ can be used to overcome the difficulties of studying very short sequences and has greatly improved, and in many cases enabled, specific and sensitive detection of microRNA and o ...
... applications in which standard DNA oligonucleotides or RNA riboprobes do not show sufficient affinity or specificity. LNA™ can be used to overcome the difficulties of studying very short sequences and has greatly improved, and in many cases enabled, specific and sensitive detection of microRNA and o ...
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn
... More and more human SNPs are analyzed for their potential association with diseases, risk factors and predispositions. Our LightSNiP assays are preestablished, probe-based tests using a melting curve to detect sequence variations. These assays are developed on the Roche LightCycler® 480 system, but ...
... More and more human SNPs are analyzed for their potential association with diseases, risk factors and predispositions. Our LightSNiP assays are preestablished, probe-based tests using a melting curve to detect sequence variations. These assays are developed on the Roche LightCycler® 480 system, but ...
Genetic tools for manipulating Acinetobacter baumannii genome: an
... A. baumannii; therefore, they can serve as integration vectors. In addition, some other E. coli plasmids, such as those derived from R6K, also do not replicate in A. baumannii. Some commonly and recently used integration plasmids for manipulation of the A. baumannii genome are listed in Jacobs et al ...
... A. baumannii; therefore, they can serve as integration vectors. In addition, some other E. coli plasmids, such as those derived from R6K, also do not replicate in A. baumannii. Some commonly and recently used integration plasmids for manipulation of the A. baumannii genome are listed in Jacobs et al ...
A systems biology approach sheds new light on the regulation of
... supported the view that acid resistance involves a mechanism based on the transcriptional switch between the expression of genes encoding aerobic and anaerobic enzymes and controlled by the two-component system (TCS) regulator OmpR. Model validation confirmed this hypothesis and provided the means t ...
... supported the view that acid resistance involves a mechanism based on the transcriptional switch between the expression of genes encoding aerobic and anaerobic enzymes and controlled by the two-component system (TCS) regulator OmpR. Model validation confirmed this hypothesis and provided the means t ...
Global transcriptional control by glucose and
... in the glucose-dependent repression of C. difficile toxin production. This repression is because of a direct binding of CcpA to the regulatory region of the tcdA and tcdB genes (30). This is the first evidence that CcpA acts as a link between carbon source utilization and virulence gene expression in ...
... in the glucose-dependent repression of C. difficile toxin production. This repression is because of a direct binding of CcpA to the regulatory region of the tcdA and tcdB genes (30). This is the first evidence that CcpA acts as a link between carbon source utilization and virulence gene expression in ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... Siddavattam, and Klingmüller 1995), or in CO2-fixation as pyruvate synthase (Chlorobium) (Yoon et al. 1999). On the other hand, among eukaryotes, PFO is only known to function in oxidative pyruvate decarboxylation in amitochondriate protists, where the electrons are donated to Fdox in hydrogenosome ...
... Siddavattam, and Klingmüller 1995), or in CO2-fixation as pyruvate synthase (Chlorobium) (Yoon et al. 1999). On the other hand, among eukaryotes, PFO is only known to function in oxidative pyruvate decarboxylation in amitochondriate protists, where the electrons are donated to Fdox in hydrogenosome ...
Pseudouridine at position 55 in tRNA controls the contents of other
... tRNA is produced by TruB. To clarify the role of the )55 modification, we constructed a truB gene disruptant ("truB) strain of Thermus thermophilus which is an extreme-thermophilic eubacterium. Unexpectedly, the "truB strain exhibited severe growth retardation at 50 C. We assumed that these phenome ...
... tRNA is produced by TruB. To clarify the role of the )55 modification, we constructed a truB gene disruptant ("truB) strain of Thermus thermophilus which is an extreme-thermophilic eubacterium. Unexpectedly, the "truB strain exhibited severe growth retardation at 50 C. We assumed that these phenome ...
Late events of translation initiation in bacteria: a kinetic analysis
... fMet-tRNAfMet to the ribosome. In contrast to EF-Tu, whose interaction with aa-tRNAs depends on the presence of GTP, the interaction of IF2 with fMet-tRNAfMet is not affected by nucleotides (Petersen et al., 1979); only the af®nity of IF2 for the 30S subunit is somewhat in¯uenced by the nature of th ...
... fMet-tRNAfMet to the ribosome. In contrast to EF-Tu, whose interaction with aa-tRNAs depends on the presence of GTP, the interaction of IF2 with fMet-tRNAfMet is not affected by nucleotides (Petersen et al., 1979); only the af®nity of IF2 for the 30S subunit is somewhat in¯uenced by the nature of th ...
Short-term regulation of the mammalian pyruvate dehydrogenase
... molecules of TDP-containing pyruvate dehydrogenase (EC 1.2.4.1; abbreviated E1) are joined to the binding domains of E2 (Reed, 2001; Fries et al., 2003). Twelve homodimeric molecules of FAD-containing dihydrolipoamide dehydrogenase (EC 1.8.1.4; abbreviated E3) are connected with the binding domains ...
... molecules of TDP-containing pyruvate dehydrogenase (EC 1.2.4.1; abbreviated E1) are joined to the binding domains of E2 (Reed, 2001; Fries et al., 2003). Twelve homodimeric molecules of FAD-containing dihydrolipoamide dehydrogenase (EC 1.8.1.4; abbreviated E3) are connected with the binding domains ...
Ethylene is involved in strawberry fruit ripening in an organ
... 1997; Aharoni and O’Connell, 2002; Bombarely et al., 2010; Csukasi et al., 2011, 2012), little is known about the regulation of these processes, including the role played by the various phytohormones. The main reason for this relatively poor understanding in comparison with other fruits is that what ...
... 1997; Aharoni and O’Connell, 2002; Bombarely et al., 2010; Csukasi et al., 2011, 2012), little is known about the regulation of these processes, including the role played by the various phytohormones. The main reason for this relatively poor understanding in comparison with other fruits is that what ...
A Point Mutation in Transthyretin Increases Affinity for Thyroxine and
... TTR purification, competitive binding studies, and protein sequencing. TTR was purified by three-step column chromatography as described previously (9). TTR affinity for thyroxine was assessed with a competitive binding assay using both whole sera (1:25 dilution in PBS) and purified TTR (0.5 ,ug/tub ...
... TTR purification, competitive binding studies, and protein sequencing. TTR was purified by three-step column chromatography as described previously (9). TTR affinity for thyroxine was assessed with a competitive binding assay using both whole sera (1:25 dilution in PBS) and purified TTR (0.5 ,ug/tub ...
The tyrosine regulated DAHP synthase and the biosynthetic
... ARO4, that can be feedback-inhibited by phenylalanine and tyrosine, respectively. Crystal structures of the phenylalanine regulated DAHP synthase of Escherichia coli and of the tyrosine inhibitable isoenzyme of S. cerevisiae are available. With the help of amino acid substitutions at the catalytic c ...
... ARO4, that can be feedback-inhibited by phenylalanine and tyrosine, respectively. Crystal structures of the phenylalanine regulated DAHP synthase of Escherichia coli and of the tyrosine inhibitable isoenzyme of S. cerevisiae are available. With the help of amino acid substitutions at the catalytic c ...
Design considerations for highly specific and efficient
... gaps in the RNA or the DNA strand were analyzed for off-target cleavage with a mismatch detection assay (using T7EI endonuclease). Red nucleotides indicate mismatches, red dashes indicate gaps, and underlined red nucleotides indicate insertions relative to the target DNA sequence. The off-targets we ...
... gaps in the RNA or the DNA strand were analyzed for off-target cleavage with a mismatch detection assay (using T7EI endonuclease). Red nucleotides indicate mismatches, red dashes indicate gaps, and underlined red nucleotides indicate insertions relative to the target DNA sequence. The off-targets we ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... transmitted by arthropod vectors to man and animals, in Which they may cause serious disease. The pathogens caus ...
... transmitted by arthropod vectors to man and animals, in Which they may cause serious disease. The pathogens caus ...