Table xx - Hindawi
... chicken lines, but the direction seems uncertain. Following Salmonella infection both lines react to Salmonella infection with induced expression of several genes of this pathway suggesting that apoptosis is induced as a reaction to infection. This pathway shows differential expression between the l ...
... chicken lines, but the direction seems uncertain. Following Salmonella infection both lines react to Salmonella infection with induced expression of several genes of this pathway suggesting that apoptosis is induced as a reaction to infection. This pathway shows differential expression between the l ...
Cloning and functional expression of invertebrate connexins from
... Regardless of whether innexins and pannexins are related, there has persisted a widely held belief that the connexins were restricted to vertebrate animals [9,13–15]. This view further assumed that all invertebrate gap junctions would be encoded by innexins, and the name innexin itself was originall ...
... Regardless of whether innexins and pannexins are related, there has persisted a widely held belief that the connexins were restricted to vertebrate animals [9,13–15]. This view further assumed that all invertebrate gap junctions would be encoded by innexins, and the name innexin itself was originall ...
Regulation of Arabidopsis 14-3
... some metabolic enzymes such as nitrate reductase in tobacco (Stitt et al. 2002) and glutamine synthetase in Arabidopsis (Oliveira & Coruzzi 1999). However, our results showed that the transcriptional response of 14-3-3 genes is specific to GABA. This also argues against the possibility that the obse ...
... some metabolic enzymes such as nitrate reductase in tobacco (Stitt et al. 2002) and glutamine synthetase in Arabidopsis (Oliveira & Coruzzi 1999). However, our results showed that the transcriptional response of 14-3-3 genes is specific to GABA. This also argues against the possibility that the obse ...
Snorks Lab File
... protein molecules and that this is virtually the same mechanism for all life forms. ...
... protein molecules and that this is virtually the same mechanism for all life forms. ...
5 DNA Replication
... home,‖ is whispered to a child, who runs to a second child and repeats the message. The message is relayed from child to child around the schoolyard until it returns to the original sender. Inevitably, the last child returns with an amazingly transformed message, such as ―Joe Brown has a pig living ...
... home,‖ is whispered to a child, who runs to a second child and repeats the message. The message is relayed from child to child around the schoolyard until it returns to the original sender. Inevitably, the last child returns with an amazingly transformed message, such as ―Joe Brown has a pig living ...
Identification and Analysis of Dicer Associated Proteins in
... complementarity, and either promote translational inhibition or degradation of their target mRNAs (for review see: (Filipowicz et al., 2005)). Foremost, the 5’ end of the miRNA, e.g. nt 2 to 8, shows almost perfect complementarity to its target site, and was termed a “seed region”. Functional miRNA ...
... complementarity, and either promote translational inhibition or degradation of their target mRNAs (for review see: (Filipowicz et al., 2005)). Foremost, the 5’ end of the miRNA, e.g. nt 2 to 8, shows almost perfect complementarity to its target site, and was termed a “seed region”. Functional miRNA ...
In situ hybridization
... expensive to use, Easier to work with, More specific, Better tissue penetration, Better reproducibility and a wide range of labeling methods that do not interfere with target detection. 1. Stability. Oligonucleotide probes are very stable. They are not degraded by RNases. Once tissue is fixed, solut ...
... expensive to use, Easier to work with, More specific, Better tissue penetration, Better reproducibility and a wide range of labeling methods that do not interfere with target detection. 1. Stability. Oligonucleotide probes are very stable. They are not degraded by RNases. Once tissue is fixed, solut ...
The aconitase of Escherichia cok purification of the
... carried out according to Southern (1975) and 1 plaques and colonies containing pGS447 and pGS448 were transferred and fixed to nitrocellulose as described by Maniatis et al. (1982). A transfer procedure which avoids depurination (Meinkoth & Wahl, 1984) was used with the oligonucleotide probes. The c ...
... carried out according to Southern (1975) and 1 plaques and colonies containing pGS447 and pGS448 were transferred and fixed to nitrocellulose as described by Maniatis et al. (1982). A transfer procedure which avoids depurination (Meinkoth & Wahl, 1984) was used with the oligonucleotide probes. The c ...
Probing Allosteric Binding Sites of the Maize
... functions as a dimer of dimers, similar to the mechanism proposed for the Escherichia coli enzyme on the basis of ligand-binding studies (Haugen and Preiss, 1979). All available evidence leads to the conclusion that tetramers are required for AGPase catalytic activity. Both available AGPase crystal ...
... functions as a dimer of dimers, similar to the mechanism proposed for the Escherichia coli enzyme on the basis of ligand-binding studies (Haugen and Preiss, 1979). All available evidence leads to the conclusion that tetramers are required for AGPase catalytic activity. Both available AGPase crystal ...
Trade-offs between tRNA abundance and mRNA secondary
... Translation of mRNAs into protein is crucial for cell viability and function and proceeds at a non-uniform rate along transcripts (1). While much focus has been placed on the translation initiation step that is often rate limiting for endogenous genes (2,3), there is growing realization that the var ...
... Translation of mRNAs into protein is crucial for cell viability and function and proceeds at a non-uniform rate along transcripts (1). While much focus has been placed on the translation initiation step that is often rate limiting for endogenous genes (2,3), there is growing realization that the var ...
Single Processing Center Models for Human Dicer and Bacterial
... double-stranded RNAs (dsRNAs) to 21 nt small interfering RNAs (siRNAs) during RNA interference, and excises microRNAs from precursor hairpins. Dicer contains two domains related to the bacterial dsRNAspecific endonuclease, RNase III, which is known to function as a homodimer. Based on an X-ray struc ...
... double-stranded RNAs (dsRNAs) to 21 nt small interfering RNAs (siRNAs) during RNA interference, and excises microRNAs from precursor hairpins. Dicer contains two domains related to the bacterial dsRNAspecific endonuclease, RNase III, which is known to function as a homodimer. Based on an X-ray struc ...
Engineering Cytosolic Acetyl-CoA Metabolism in Saccharomyces
... could provide the cells with sufficient precursors for cytosolic acetyl-CoA. Here we investigated this using a Pdc negative strain with MTH1-∆T, IMI076. Our results identified a route relying on Ach1 that could transfer acetyl units from mitochondria to the cytoplasm. Based on the results a new mode ...
... could provide the cells with sufficient precursors for cytosolic acetyl-CoA. Here we investigated this using a Pdc negative strain with MTH1-∆T, IMI076. Our results identified a route relying on Ach1 that could transfer acetyl units from mitochondria to the cytoplasm. Based on the results a new mode ...
RNA interference - Bio
... (PTGS) in plants1 and later independently identified in wide variety of eukaryotic organisms. These dsRNAs are rapidly processed into short RNA duplexes of 21 to 28 nucleotides in length, which then guide the recognition and ultimately the cleavage of complementary single-stranded RNAs, such as mess ...
... (PTGS) in plants1 and later independently identified in wide variety of eukaryotic organisms. These dsRNAs are rapidly processed into short RNA duplexes of 21 to 28 nucleotides in length, which then guide the recognition and ultimately the cleavage of complementary single-stranded RNAs, such as mess ...
PDF File
... Tetrahymena group I RNA enzyme described herein directly demonstrate the catalytic contributions of binding interactions. With wild-type ribozyme, specific functional groups at a distance from the site of chemical transformation facilitate substrate binding without accelerating reaction of bound sub ...
... Tetrahymena group I RNA enzyme described herein directly demonstrate the catalytic contributions of binding interactions. With wild-type ribozyme, specific functional groups at a distance from the site of chemical transformation facilitate substrate binding without accelerating reaction of bound sub ...
On Periodicity in the Occurrence of Nucleotides in Protein Coding
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
E. coli
... It is proposed by Pickett et al. that the LTp and LTh (antigenic variants of LT will both be included in serogroup I and should be designated LTp-I and LTh-I and the LT-like toxin will be the prototype for serogroup II enterotoxins and should be renamed LT-II. Two distinct members of the LT-II fam ...
... It is proposed by Pickett et al. that the LTp and LTh (antigenic variants of LT will both be included in serogroup I and should be designated LTp-I and LTh-I and the LT-like toxin will be the prototype for serogroup II enterotoxins and should be renamed LT-II. Two distinct members of the LT-II fam ...
USMLE Step 1 - Becker Professional Education
... of State Medical Boards (FSMB) and National Board of Medical Examiners® (NBME®). United States Medical Licensing Examination, USMLE, National Board of Medical Examiners, and NBME are registered trademarks of the National Board of Medical Examiners. The National Board of Medical Examiners does not sp ...
... of State Medical Boards (FSMB) and National Board of Medical Examiners® (NBME®). United States Medical Licensing Examination, USMLE, National Board of Medical Examiners, and NBME are registered trademarks of the National Board of Medical Examiners. The National Board of Medical Examiners does not sp ...
University of Groningen Interactions between carbohydrate
... Metabolic fluxes, which can be defined as the rate of flow of given molecules/substrates through defined biochemical processes that occur within a living organism, need to be regulated to maintain homeostasis at a cellular level. One can look at regulation of metabolism in many ways, but it is illus ...
... Metabolic fluxes, which can be defined as the rate of flow of given molecules/substrates through defined biochemical processes that occur within a living organism, need to be regulated to maintain homeostasis at a cellular level. One can look at regulation of metabolism in many ways, but it is illus ...
Full-Text PDF
... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
P003 MLH1 / MSH2 EXON DELETION TEST KIT:
... The PM200 Mouse control probemix contains 6 different probes with amplification products between 175 and 545 nt, as well as 4 control fragments generating an amplification product smaller than 90 nt. In many cases, resul;ts can be confirmed. Southern blot, FISH or long range PCR. The length of the a ...
... The PM200 Mouse control probemix contains 6 different probes with amplification products between 175 and 545 nt, as well as 4 control fragments generating an amplification product smaller than 90 nt. In many cases, resul;ts can be confirmed. Southern blot, FISH or long range PCR. The length of the a ...
... autosomes and two sex chromosomes. Each chromosome is thought to contain one linear DNA molecule featuring three functional elements required for the successful duplication of the chromosome upon cell division: (i) autonomous replication sequences; (ii) the centromere, to which the mitotic or meioti ...
In Vitro Characterization of Human Growth Hormone
... Growth hormone (GH) is a 22 kD, 191-aa, pituitary-derived peptide hormone that is essential for postnatal growth. GH signals via binding to GH receptor (GHR), which initiates intracellular signal transduction pathways. This leads to activation of target genes, most importantly the one encoding insul ...
... Growth hormone (GH) is a 22 kD, 191-aa, pituitary-derived peptide hormone that is essential for postnatal growth. GH signals via binding to GH receptor (GHR), which initiates intracellular signal transduction pathways. This leads to activation of target genes, most importantly the one encoding insul ...
Virology Journal
... < 0.001), 40 out of 54 (74%), 37 out of 52 (71%) and 36 out of 52 (69%) of the ORFs for phages eiAU, eiDWF, and eiMSLS, respectively, share significant sequence similarity to known protein sequences contained in the GenBank nr/nt database (Table 1). Of the ORFs with significant sequence similarity t ...
... < 0.001), 40 out of 54 (74%), 37 out of 52 (71%) and 36 out of 52 (69%) of the ORFs for phages eiAU, eiDWF, and eiMSLS, respectively, share significant sequence similarity to known protein sequences contained in the GenBank nr/nt database (Table 1). Of the ORFs with significant sequence similarity t ...
bio98a_l10
... the allosteric activator; this may seem strange but the net objective of glycolysis is generation of ATP. If the ATP levels drop (ie [ADP] goes up), glycolysis needs to be stimulated. Thus ADP binds to and activates phosphofructokinase-1 to increase ...
... the allosteric activator; this may seem strange but the net objective of glycolysis is generation of ATP. If the ATP levels drop (ie [ADP] goes up), glycolysis needs to be stimulated. Thus ADP binds to and activates phosphofructokinase-1 to increase ...
A Pneumocystis carinii multi-gene family with
... Four recombinant phage isolated from the cDNA library were partially characterized. The recombinant DNA was recovered from the 1 phage by in vivo excision as pBluescript plasmid DNA. The size of the recombinant DNA ranged from 2-7kb to 2.9 kb, and sequence analysis revealed that all four clones cont ...
... Four recombinant phage isolated from the cDNA library were partially characterized. The recombinant DNA was recovered from the 1 phage by in vivo excision as pBluescript plasmid DNA. The size of the recombinant DNA ranged from 2-7kb to 2.9 kb, and sequence analysis revealed that all four clones cont ...