Biochimica et Biophysica Acta
... the coordinate file 1T0K.pdb [13,30]. In silico single residue mutations were made and energy minimized locally. The program ENTANGLE was used to aid in cataloguing interactions between the RNA and protein [31]. 3. Results 3.1. Preparation of the two-plasmid system A bacterial two-plasmid system has ...
... the coordinate file 1T0K.pdb [13,30]. In silico single residue mutations were made and energy minimized locally. The program ENTANGLE was used to aid in cataloguing interactions between the RNA and protein [31]. 3. Results 3.1. Preparation of the two-plasmid system A bacterial two-plasmid system has ...
Modeling RNA Molecules
... who said, “The purpose of computing is insight, not numbers” (Hamming 1971), we should remember that the purpose of molecular modeling is functional insight, not detailed atomic models per se. Therefore, as we seek to improve our abilities to construct 3D models for molecules for which we do not yet ...
... who said, “The purpose of computing is insight, not numbers” (Hamming 1971), we should remember that the purpose of molecular modeling is functional insight, not detailed atomic models per se. Therefore, as we seek to improve our abilities to construct 3D models for molecules for which we do not yet ...
Regulation of gene expression by Pax6 in ocular cells: a case of
... sense mutants, R26G and R128C, in transient reporter assays revealed that both PAI and RED subdomains of the PD could 5'-half recognized by the PAI subdomain and its 3'-half recognegatively regulate transactivation of both Pax6 and Pax6(5a) denized by the RED subdomain (Czerny et al., 1993; Epstein ...
... sense mutants, R26G and R128C, in transient reporter assays revealed that both PAI and RED subdomains of the PD could 5'-half recognized by the PAI subdomain and its 3'-half recognegatively regulate transactivation of both Pax6 and Pax6(5a) denized by the RED subdomain (Czerny et al., 1993; Epstein ...
Two Genes with Similarity to Bacterial Response Regulators Are
... database whose sequence is 69% identical and 95% similar to the IBC6 amino acid sequence (Figure 1C). This rice gene also possesses a C-terminal extension after the receiver domain, although it is not similar to the sequence of the IBC7 extension (data not shown). The highly conserved residues of re ...
... database whose sequence is 69% identical and 95% similar to the IBC6 amino acid sequence (Figure 1C). This rice gene also possesses a C-terminal extension after the receiver domain, although it is not similar to the sequence of the IBC7 extension (data not shown). The highly conserved residues of re ...
Spatial restriction of AChR gene expression to
... nerve and is caused, in part, by post-translational mechanisms that involve a redistribution of AChR protein in the myotube membrane. We have used transgenic mice that harbor a gene fusion between the murine AChR delta subunit gene and the human growth hormone gene to show that innervation also acti ...
... nerve and is caused, in part, by post-translational mechanisms that involve a redistribution of AChR protein in the myotube membrane. We have used transgenic mice that harbor a gene fusion between the murine AChR delta subunit gene and the human growth hormone gene to show that innervation also acti ...
Molecular Microbial Ecology of the Rhizosphere
... trpED) and two other strains bearing knock-out mutations in genes probably involved in IAA synthesis, ysnE (putative IAA transacetylase) and yhcX (putative nitrilase), produce smaller amounts of IAA than wild type. Three of these mutant strains are less efficient in promoting plant growth (Idris ...
... trpED) and two other strains bearing knock-out mutations in genes probably involved in IAA synthesis, ysnE (putative IAA transacetylase) and yhcX (putative nitrilase), produce smaller amounts of IAA than wild type. Three of these mutant strains are less efficient in promoting plant growth (Idris ...
440origin - eweb.furman.edu
... genetic similarity in genes involved in particular pathways (suggesting gene duplication and subsequent evolution of new genes and elaboration of existing pathways) 5. Evolution of a genetic system - a reasonable hypothesis and significant corroborating evidence that it could happen. But no experime ...
... genetic similarity in genes involved in particular pathways (suggesting gene duplication and subsequent evolution of new genes and elaboration of existing pathways) 5. Evolution of a genetic system - a reasonable hypothesis and significant corroborating evidence that it could happen. But no experime ...
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... and the receptors used for its detection. In gram negative bacteria typically use Lux I/R quorum-sensing (Yang, ...
... and the receptors used for its detection. In gram negative bacteria typically use Lux I/R quorum-sensing (Yang, ...
Life and death of transcriptional co
... proteasome system in response to toxic proteins.65 The formation of aggresome is a cellular protective mechanism to sequester Increasing evidence has provided functional implications of cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigen ...
... proteasome system in response to toxic proteins.65 The formation of aggresome is a cellular protective mechanism to sequester Increasing evidence has provided functional implications of cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigen ...
Green Fluorescent Protein (GFP) Purification Kit
... One of the basic tools of modern biotechnology is DNA splicing, cutting DNA and linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the prot ...
... One of the basic tools of modern biotechnology is DNA splicing, cutting DNA and linking it to other DNA molecules. The basic concept behind DNA splicing is to remove a functional DNA fragment—let’s say a gene—from one organism and combine it with the DNA of another organism in order to make the prot ...
Type="Italic">AtGRP7 is involved in the
... responses. However, the analysis of the motif sequence revealed that the promoter of AtGRP7 did not contain an ABRE (www.arabidopsis.leeds.ac.uk/ act/cislocator.php), suggesting that AtGRP7 may be indirectly mediated by ABA. As hyperosmotic stress and high salt induce the production of ABA in plants ...
... responses. However, the analysis of the motif sequence revealed that the promoter of AtGRP7 did not contain an ABRE (www.arabidopsis.leeds.ac.uk/ act/cislocator.php), suggesting that AtGRP7 may be indirectly mediated by ABA. As hyperosmotic stress and high salt induce the production of ABA in plants ...
Acquisition of 1,000 eubacterial genes physiologically transformed a
... groups (Fig. 2C) reveals that merely four eubacterial imports (three predicted membrane proteins and a glycosyl transferase) can be traced to their common ancestor, and that these are present in at most 6 of the 22 descendant genomes. Whereas 124 imports can be traced to the Ms/Mc/Mm common ancestor ...
... groups (Fig. 2C) reveals that merely four eubacterial imports (three predicted membrane proteins and a glycosyl transferase) can be traced to their common ancestor, and that these are present in at most 6 of the 22 descendant genomes. Whereas 124 imports can be traced to the Ms/Mc/Mm common ancestor ...
Acetyl-coenzyme A carboxylase – an attractive enzyme for biotechnolo
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
Acetyl-coenzyme A carboxylase – an attractive enzyme for biotechnolo
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
Characterisation of the diol dehydratase pdu operon of Lactobacillus
... greater speci¢city for glycerol and a⁄nity for the coenzyme B-12 than diol dehydratase [4]. Glycerol dehydratase of K. pneumoniae and C. freundii form part of the dha regulon induced under anaerobic conditions by glycerol and dihydroxyacetone. The diol dehydratase genes of S. enterica are part of th ...
... greater speci¢city for glycerol and a⁄nity for the coenzyme B-12 than diol dehydratase [4]. Glycerol dehydratase of K. pneumoniae and C. freundii form part of the dha regulon induced under anaerobic conditions by glycerol and dihydroxyacetone. The diol dehydratase genes of S. enterica are part of th ...
Function and specificity of 14-3-3 proteins in the regulation of
... phosphorylation of SPS on Ser-424 and Ser-158 which are thought to be responsible for light/dark modulation and osmotic stress activation of the enzyme (McMichael et al., 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spi ...
... phosphorylation of SPS on Ser-424 and Ser-158 which are thought to be responsible for light/dark modulation and osmotic stress activation of the enzyme (McMichael et al., 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spi ...
The origin of the RNA world: Co-evolution of genes and metabolism
... 2 0 -OH of the terminal A of the tRNA substrate accelerates the rate of peptide bond formation by 106 [19]. This simple and effective strategy may have been preserved since the origin of the ribosome. 3. A metabolic module for synthesis of nucleotides Extant autotrophs utilize a universal core of met ...
... 2 0 -OH of the terminal A of the tRNA substrate accelerates the rate of peptide bond formation by 106 [19]. This simple and effective strategy may have been preserved since the origin of the ribosome. 3. A metabolic module for synthesis of nucleotides Extant autotrophs utilize a universal core of met ...
Phylogenetic relationship of phototrophic purple sulfur bacteria
... can be easily distinguished by their ability to store elemental sulfur inside or outside the cells as an intermediate during photosynthesis. Functional gene approaches can support phylogenetic considerations concluded from 16S rRNA gene analysis. This has already been demonstrated for the FennaMatht ...
... can be easily distinguished by their ability to store elemental sulfur inside or outside the cells as an intermediate during photosynthesis. Functional gene approaches can support phylogenetic considerations concluded from 16S rRNA gene analysis. This has already been demonstrated for the FennaMatht ...
Chapter 15 The Techniques of Molecular Genetics
... samples of specific segments of chromosomes. Gel electrophoresis procedures able to resolve DNA fragments differing in length by a single nucleotide. Gene-cloning techniques allowing preparation of large quantities of a DNA molecule. Sanger sequencing Technique is used to determine ...
... samples of specific segments of chromosomes. Gel electrophoresis procedures able to resolve DNA fragments differing in length by a single nucleotide. Gene-cloning techniques allowing preparation of large quantities of a DNA molecule. Sanger sequencing Technique is used to determine ...
A Brief History of PCR - Bio-Rad
... Mullis, born in Lenoir, North Carolina, attended the University of Georgia Tech for his undergraduate work in chemistry, and then obtained a Ph. D. in biochemistry from Cal Berkeley. In 1983, working for Cetus Corporation, Mullis developed the Polymerase Chain Reaction, a technique for the rapid syn ...
... Mullis, born in Lenoir, North Carolina, attended the University of Georgia Tech for his undergraduate work in chemistry, and then obtained a Ph. D. in biochemistry from Cal Berkeley. In 1983, working for Cetus Corporation, Mullis developed the Polymerase Chain Reaction, a technique for the rapid syn ...
A Histone H3.3-like Gene Specifically Expressed in the Vegetative
... anthers and mature pollen of Lilium longiflorum. Northern blot and reverse transcription–PCR (RT–PCR) analyses demonstrated that YAH3 transcripts were present in uninucleate microspores and generative cells at the postulated S phase of the cell cycle as well as in young anthers, meristematic root ti ...
... anthers and mature pollen of Lilium longiflorum. Northern blot and reverse transcription–PCR (RT–PCR) analyses demonstrated that YAH3 transcripts were present in uninucleate microspores and generative cells at the postulated S phase of the cell cycle as well as in young anthers, meristematic root ti ...
Protein Synthesis
... Bonding Between DNA & RNA When an RNA is made from DNA, the DNA bases and the RNA bases bond. If the DNA base is Guanine, RNA Cytosine bonds to it and vice versa. If the DNA base is Thymine, RNA Adenine bonds to it. BUT if the DNA base is Adenine, RNA Uracil will bond to it. ...
... Bonding Between DNA & RNA When an RNA is made from DNA, the DNA bases and the RNA bases bond. If the DNA base is Guanine, RNA Cytosine bonds to it and vice versa. If the DNA base is Thymine, RNA Adenine bonds to it. BUT if the DNA base is Adenine, RNA Uracil will bond to it. ...
Chpt14_Translation.doc
... ribosome ready for the elongation phase of translation. Note that f-mettRNAfmet is positioned at the P site. It has recognized the initiator AUG in the mRNA. 4. Identification of initiator AUG in eukaryotes a. Bases around AUG influence efficiency of initiation. (1) The most important effects are fr ...
... ribosome ready for the elongation phase of translation. Note that f-mettRNAfmet is positioned at the P site. It has recognized the initiator AUG in the mRNA. 4. Identification of initiator AUG in eukaryotes a. Bases around AUG influence efficiency of initiation. (1) The most important effects are fr ...
ppt - eweb.furman.edu
... water in an exothermic reaction, giving off heat and H2. The warm (60-90oC), H-rich water percolates up and the carbonate minerals precipitate into microporous chimneys. High flux of carbon and energy is channeled over inorganic catalysts Thermal currents through pores can concentrate organics 1000 ...
... water in an exothermic reaction, giving off heat and H2. The warm (60-90oC), H-rich water percolates up and the carbonate minerals precipitate into microporous chimneys. High flux of carbon and energy is channeled over inorganic catalysts Thermal currents through pores can concentrate organics 1000 ...
REVIEWS
... carbon, nitrogen, phosphorus and sulphur. This enables them to feed the central metabolic pathways — glycolysis, the pentose-phosphate pathway, the citric acid cycle and the 2‑oxoglutarate–glutamate–glutamine cycle — from which all of the precursors that are required for the synthesis of the cell’s ...
... carbon, nitrogen, phosphorus and sulphur. This enables them to feed the central metabolic pathways — glycolysis, the pentose-phosphate pathway, the citric acid cycle and the 2‑oxoglutarate–glutamate–glutamine cycle — from which all of the precursors that are required for the synthesis of the cell’s ...