PDF - Oxford Academic
... acid sequence analysis has not yet been completed on homogenous Lbc varieties (Whittaker, R.G., personal communication). DNA sequences in both genes corresponding to restriction enzyme cleavage sites were verified by cleavage with the appropriate restriction enzyme with one exception. In the Lbc gen ...
... acid sequence analysis has not yet been completed on homogenous Lbc varieties (Whittaker, R.G., personal communication). DNA sequences in both genes corresponding to restriction enzyme cleavage sites were verified by cleavage with the appropriate restriction enzyme with one exception. In the Lbc gen ...
Preparing and Analyzing Expressed Sequence Tags (ESTs) Library
... done with the CAP3 software. According to CAP3 result the prenatal stage EST sequences into 23 contigs and postnatal stage EST sequences into 27 contigs were assembled. Furthermore, in prenatal stage contigs, the longest contig is 1394 bp, it is 2068 in postnatal stage contig. The number of singlets ...
... done with the CAP3 software. According to CAP3 result the prenatal stage EST sequences into 23 contigs and postnatal stage EST sequences into 27 contigs were assembled. Furthermore, in prenatal stage contigs, the longest contig is 1394 bp, it is 2068 in postnatal stage contig. The number of singlets ...
View PDF - DNA and Natural Algorithms Group
... and stacking are the two dominant factors for affinity and discrimination. While there is a simple and common motif for adenine binding, other sequences and structures are also possible (just as there are other ways that proteins can interact with adenosine other than the Rossman fold). An anti-CoA ...
... and stacking are the two dominant factors for affinity and discrimination. While there is a simple and common motif for adenine binding, other sequences and structures are also possible (just as there are other ways that proteins can interact with adenosine other than the Rossman fold). An anti-CoA ...
BI 112 Instructor: Waite Exam #4 Study Guide Cell Membrane
... Understand how RNA is processed after transcription before it is translated; know the difference between introns and exons, which one codes for protein, and the 2 main advantages of storing coding information in this way ...
... Understand how RNA is processed after transcription before it is translated; know the difference between introns and exons, which one codes for protein, and the 2 main advantages of storing coding information in this way ...
Conceptual Questions C1. Answer: The start codon begins at the
... C11. Answer: An anticodon that was 3′–UUG–5′ would recognize the two codons. To recognize 5′–AAA–3′, it would have to be modified to 3′–UUI–5′. C12. Answer: All tRNA molecules have some basic features in common. They all have a cloverleaf structure with three stem-loop structures. The second stem-l ...
... C11. Answer: An anticodon that was 3′–UUG–5′ would recognize the two codons. To recognize 5′–AAA–3′, it would have to be modified to 3′–UUI–5′. C12. Answer: All tRNA molecules have some basic features in common. They all have a cloverleaf structure with three stem-loop structures. The second stem-l ...
Diabetes (type II) treatment, Dec. 7
... – Investigate how T2384 reduces fat accumulation and increases insulin sensitivity – Create and explore other drugs through structurebased drug design that bind to S pocket and note effects on PPARγ ...
... – Investigate how T2384 reduces fat accumulation and increases insulin sensitivity – Create and explore other drugs through structurebased drug design that bind to S pocket and note effects on PPARγ ...
Chromatin Condensing Functions of the Linker Histone C
... order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered protein domains in eukaryotic proteomes (23), there is a relative paucity of experimental biochemical data that address the ...
... order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered protein domains in eukaryotic proteomes (23), there is a relative paucity of experimental biochemical data that address the ...
Chapter 2 - Institut Montefiore
... DNA: the master molecule of every cell It contains vital information that gets passed on to each successive generation. It coordinates the making of itself as well as other molecules (proteins). If it is changed slightly, serious consequences may result. If it is destroyed beyond repair, the cell ...
... DNA: the master molecule of every cell It contains vital information that gets passed on to each successive generation. It coordinates the making of itself as well as other molecules (proteins). If it is changed slightly, serious consequences may result. If it is destroyed beyond repair, the cell ...
The mechanism of redox sensing in Mycobacterium tuberculosis
... of protective mechanisms and enzymes, including superoxide dismutase, catalase (KatG), alkyl hydroperoxidase (AhpC), and peroxiredoxins, to neutralize the redox stress generated by the macrophage cells [2]. In addition, the mycolic acid-rich cell wall of Mtb also protects against ROS. Mtb maintains ...
... of protective mechanisms and enzymes, including superoxide dismutase, catalase (KatG), alkyl hydroperoxidase (AhpC), and peroxiredoxins, to neutralize the redox stress generated by the macrophage cells [2]. In addition, the mycolic acid-rich cell wall of Mtb also protects against ROS. Mtb maintains ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... Bacillus phage29. The similarity between these conserved regions is
most striking in their linear spatial arrangement on each pol polypeptide.
The regions are designated I-VI by their extent of similarity, with region
I being the most conserved (Wong et al. 1988). Delarue et al. designated
the t ...
... Bacillus phage
p53 regulation and function in normal cells and tumors
... loss of p53 function has been estimated to occur in almost 80% of human cancers, due not only to mutation but also to defects in activating events or inactivation by the products of tumor viruses or cellular oncogenes. We became focused on wild type p53 protein loss of function due to observations t ...
... loss of p53 function has been estimated to occur in almost 80% of human cancers, due not only to mutation but also to defects in activating events or inactivation by the products of tumor viruses or cellular oncogenes. We became focused on wild type p53 protein loss of function due to observations t ...
Novel surface layer protein genes in Bacillus
... The cells of B. sphaericus JG-A12, like those of the closely related B. sphaericus NCTC 9602, are able to bind selectively and reversibly high amounts of metals such as uranium, lead, copper, aluminium, gallium and cadmium from drain waters of uranium wastes (Selenska-Pobell et al., 1999). Further a ...
... The cells of B. sphaericus JG-A12, like those of the closely related B. sphaericus NCTC 9602, are able to bind selectively and reversibly high amounts of metals such as uranium, lead, copper, aluminium, gallium and cadmium from drain waters of uranium wastes (Selenska-Pobell et al., 1999). Further a ...
Slides
... (pathway-inputs pwy), (pathway-outputs pwy) Returns all compounds consumed (produced) but not produced (consumed) by pathway (ignores stoichiometry) ...
... (pathway-inputs pwy), (pathway-outputs pwy) Returns all compounds consumed (produced) but not produced (consumed) by pathway (ignores stoichiometry) ...
Novel regulatory roles of cAMP receptor proteins in
... catabolite repression (CCR) is a vital part of a global control system in bacteria to achieve hierarchical carbon usage (Kovárová-Kovar & Egli, 1998). During this process, depletion of glucose leads to activation of adenylyl cyclase by the phosphorylated form of the EIIA protein of the phosphotran ...
... catabolite repression (CCR) is a vital part of a global control system in bacteria to achieve hierarchical carbon usage (Kovárová-Kovar & Egli, 1998). During this process, depletion of glucose leads to activation of adenylyl cyclase by the phosphorylated form of the EIIA protein of the phosphotran ...
Phylogenetic analysis of phytoplasmas based on sequences
... Lee et al. (1998) (IRPCM, 2004; Firrao et al., 2005). However, it is of interest to determine how a group system based on 16S rDNA similarity compares when constructed with other genes such as the 23S rDNA or less well conserved genes such as secA. Whilst there are more than 200 sequences available ...
... Lee et al. (1998) (IRPCM, 2004; Firrao et al., 2005). However, it is of interest to determine how a group system based on 16S rDNA similarity compares when constructed with other genes such as the 23S rDNA or less well conserved genes such as secA. Whilst there are more than 200 sequences available ...
Sequential Expression of Macromolecule
... Determination of phosphate utilization. Labelled phosphate [95 Ci (mg phosphate)-'] was added at inoculation time to control flasks and to flasks supplemented with 10mM-unlabelled inorganic phosphate. Samples (250 pl) of the culture were filtered through Whatman GF/A glass-fibre filters and the filt ...
... Determination of phosphate utilization. Labelled phosphate [95 Ci (mg phosphate)-'] was added at inoculation time to control flasks and to flasks supplemented with 10mM-unlabelled inorganic phosphate. Samples (250 pl) of the culture were filtered through Whatman GF/A glass-fibre filters and the filt ...
Autotaxin–Lysophosphatidic Acid Axis Acts Downstream of
... in the online-only Data Supplement), suggesting that different levels of ApoB lipoproteins result in activation of distinct gene sets within ECs. We also found that hyperlipidemia induced a more pronounced transcriptional response than lipoprotein depletion, as manifested by the larger number of gen ...
... in the online-only Data Supplement), suggesting that different levels of ApoB lipoproteins result in activation of distinct gene sets within ECs. We also found that hyperlipidemia induced a more pronounced transcriptional response than lipoprotein depletion, as manifested by the larger number of gen ...
The maize ID1 flowering time regulator is a zinc finger protein with
... deduced ID1 protein contains two zinc ®ngers similar to the Drosophila KruÈppel-like zinc ®ngersÐa C2H2 ®nger and the less prevalent C2HC zinc ®nger (14). The presence of zinc ®nger motifs and the localization of ID1 protein to the nucleus (J. Colasanti, unpublished results) suggest that ID1 binds D ...
... deduced ID1 protein contains two zinc ®ngers similar to the Drosophila KruÈppel-like zinc ®ngersÐa C2H2 ®nger and the less prevalent C2HC zinc ®nger (14). The presence of zinc ®nger motifs and the localization of ID1 protein to the nucleus (J. Colasanti, unpublished results) suggest that ID1 binds D ...
Human Gene Therapy : A Brief Overview of the Genetic
... advantages due to their low immunogenicity and their large capacity for therapeutic DNA. To improve the function of non-viral vectors, the addition of viral functions such as receptor mediated uptake and nuclear translocation of DNA may finally lead to the development of an artificial virus. Gene tr ...
... advantages due to their low immunogenicity and their large capacity for therapeutic DNA. To improve the function of non-viral vectors, the addition of viral functions such as receptor mediated uptake and nuclear translocation of DNA may finally lead to the development of an artificial virus. Gene tr ...
SAMHD1 is a single-stranded nucleic acid
... The HIV-1 restriction factor SAMHD1 is a tetrameric enzyme activated by guanine nucleotides with dNTP triphosphate hydrolase activity (dNTPase). In addition to this established activity, there have been a series of conflicting reports as to whether the enzyme also possesses single-stranded DNA and/o ...
... The HIV-1 restriction factor SAMHD1 is a tetrameric enzyme activated by guanine nucleotides with dNTP triphosphate hydrolase activity (dNTPase). In addition to this established activity, there have been a series of conflicting reports as to whether the enzyme also possesses single-stranded DNA and/o ...
Book Review - Journal of Experimental Biology
... precise. The text is complemented by schematic artwork and a number of text boxes summarizing important facts and examples. References are limited but present a well-selected and up-to-date set for additional reading. This book is unique among publications that are relevant to metabolism, primarily ...
... precise. The text is complemented by schematic artwork and a number of text boxes summarizing important facts and examples. References are limited but present a well-selected and up-to-date set for additional reading. This book is unique among publications that are relevant to metabolism, primarily ...
Microenvironment analysis and identification of magnesium binding
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
regulation of mammalian acetyl
... 23, 25, 29, 77, 79, 95, 1200, and 1215 (Figure 2). Among the identified protein kinases, only cAMP-dependent protein kinase and 50 -AMP–dependent protein kinase can inactivate ACC in vitro. cAMP-dependent protein kinase inactivates the enzyme by phosphorylation of Ser-77 and -1200, whereas 50 -AMP– ...
... 23, 25, 29, 77, 79, 95, 1200, and 1215 (Figure 2). Among the identified protein kinases, only cAMP-dependent protein kinase and 50 -AMP–dependent protein kinase can inactivate ACC in vitro. cAMP-dependent protein kinase inactivates the enzyme by phosphorylation of Ser-77 and -1200, whereas 50 -AMP– ...
Isolation and Characterization of Rice MADS Box Gene
... green or etiolated shoots, and embryos. The 35 clones were classified into 11 independent species based on their 3'-UTR sequences. Four of the 11 species corresponded to the previously reported rice MADS box genes OsMADSl,7 OsMADS2,8 OsMADS4,8 and OsMADS5.9 The remaining 7 species are newly assigned ...
... green or etiolated shoots, and embryos. The 35 clones were classified into 11 independent species based on their 3'-UTR sequences. Four of the 11 species corresponded to the previously reported rice MADS box genes OsMADSl,7 OsMADS2,8 OsMADS4,8 and OsMADS5.9 The remaining 7 species are newly assigned ...