STRIVE Report Series No.65
... P. putida CA-3 not only offers a significant styrene remediation capability but also the opportunity to use a toxic waste compound as the starting material for the production of value-added environmentally friendly bio-plastic. This project has focused mainly on the styrene-degrading ability of P. p ...
... P. putida CA-3 not only offers a significant styrene remediation capability but also the opportunity to use a toxic waste compound as the starting material for the production of value-added environmentally friendly bio-plastic. This project has focused mainly on the styrene-degrading ability of P. p ...
Choreography of Transcriptomes and Lipidomes of
... For instance, glycolysis and pentose phosphate pathways are the major contributors for pyruvate production in vascular plants, and a minor amount of pyruvate can also be synthesized from malate by NADP-dependent malic enzyme (Kang and Rawsthorne, 1996; Alonso et al., 2007). Pyruvate is then converte ...
... For instance, glycolysis and pentose phosphate pathways are the major contributors for pyruvate production in vascular plants, and a minor amount of pyruvate can also be synthesized from malate by NADP-dependent malic enzyme (Kang and Rawsthorne, 1996; Alonso et al., 2007). Pyruvate is then converte ...
R26 :: CAG GCaMP6f - The Jackson Laboratory
... OPTOGENETICS: control of cellular functions in genetically modified cells using opsins - transmembrane, retinal-binding proteins that combine a light-sensitive domain with an ion channel or pump. Upon absorption of light, the protein is activated and provides ion transport, membrane potential altera ...
... OPTOGENETICS: control of cellular functions in genetically modified cells using opsins - transmembrane, retinal-binding proteins that combine a light-sensitive domain with an ion channel or pump. Upon absorption of light, the protein is activated and provides ion transport, membrane potential altera ...
Identification of a Cis-Acting Element of ART1, a
... Rice (Oryza sativa) is one of the most aluminum (Al)-tolerant species among small-grain cereals. Recent identification of a transcription factor AL RESISTANCE TRANSCRIPTION FACTOR1 (ART1) revealed that this high Al tolerance in rice is achieved by multiple genes involved in detoxification of Al at d ...
... Rice (Oryza sativa) is one of the most aluminum (Al)-tolerant species among small-grain cereals. Recent identification of a transcription factor AL RESISTANCE TRANSCRIPTION FACTOR1 (ART1) revealed that this high Al tolerance in rice is achieved by multiple genes involved in detoxification of Al at d ...
PPT
... • Which DNA sequences encode RNA? • Which genomic DNA is "junk"? • Which RNA sequences encode proteins? Dobbs #1 - What is Bioinformatics? ...
... • Which DNA sequences encode RNA? • Which genomic DNA is "junk"? • Which RNA sequences encode proteins? Dobbs #1 - What is Bioinformatics? ...
Cloning and functional characterization of temperature responsive
... Another reason of this potential in drought tolerance could be due to its high stomatal control enabling plants to minimize the transpiration rate by keeping also a high level of net CO2 assimilation under water stress conditions (Severino et al., 2012). These physiologic ...
... Another reason of this potential in drought tolerance could be due to its high stomatal control enabling plants to minimize the transpiration rate by keeping also a high level of net CO2 assimilation under water stress conditions (Severino et al., 2012). These physiologic ...
Strategies in the interfield discovery of the mechanism of protein
... working backward from peptide bonds to the mechanisms of polypeptide assembly, focusing on chemical reactions and energy requirements for such strong covalent bonds to form. Biochemists often used in vitro experimental systems, such as Zamecnik’s cell-free rat liver preparation. As Zamecnik put it g ...
... working backward from peptide bonds to the mechanisms of polypeptide assembly, focusing on chemical reactions and energy requirements for such strong covalent bonds to form. Biochemists often used in vitro experimental systems, such as Zamecnik’s cell-free rat liver preparation. As Zamecnik put it g ...
ARTÍCULOS
... of global modulators that also includes the YmoA protein from Yersinia enterocolitica. This interaction has been found to be involved in the regulation of the expression of the toxin ␣-hemolysin. In this study, we further characterize the interaction between H-NS and Hha. We show that the presence o ...
... of global modulators that also includes the YmoA protein from Yersinia enterocolitica. This interaction has been found to be involved in the regulation of the expression of the toxin ␣-hemolysin. In this study, we further characterize the interaction between H-NS and Hha. We show that the presence o ...
Thermodynamic analysis of DNA binding by a Bacillus single
... Unlike E. coli SSB, single stranded DNA binding protein from bacteriophage T4, the gene 32 protein, is a monomer. T4 gene 32 protein can form multimers at high concentration induced by high salt and high pH [22]. Kim and Richardson demonstrated that the bacteriophage T7 SSB, the gene 2.5 protein, is ...
... Unlike E. coli SSB, single stranded DNA binding protein from bacteriophage T4, the gene 32 protein, is a monomer. T4 gene 32 protein can form multimers at high concentration induced by high salt and high pH [22]. Kim and Richardson demonstrated that the bacteriophage T7 SSB, the gene 2.5 protein, is ...
Chloroplast phosphoglycerate kinase from Euglena gracilis
... almost identical proteins of 423 amino acids that differ in only one residue. Asp422 of the second PGK protein (and also of the identical C-terminal fragment of the first unit) was replaced by Asn in the third PGK protein at the 3¢ end. At the nucleotide level sequence identity of the PGK segments is ...
... almost identical proteins of 423 amino acids that differ in only one residue. Asp422 of the second PGK protein (and also of the identical C-terminal fragment of the first unit) was replaced by Asn in the third PGK protein at the 3¢ end. At the nucleotide level sequence identity of the PGK segments is ...
Regulation of Elovl and fatty acid metabolism
... (TER) that were found to catalyse the reduction of 3-ketoacyl-CoA and trans-2,3-enoyl-CoA respectively (Moon and Horton, 2003). At that time, Ybr159p and Tsc13p had been identified as analogue reduction enzymes in yeast (Beaudoin et al., 2002; Kohlwein et al., 2001). Recently, Denic and Weissman hav ...
... (TER) that were found to catalyse the reduction of 3-ketoacyl-CoA and trans-2,3-enoyl-CoA respectively (Moon and Horton, 2003). At that time, Ybr159p and Tsc13p had been identified as analogue reduction enzymes in yeast (Beaudoin et al., 2002; Kohlwein et al., 2001). Recently, Denic and Weissman hav ...
(De)regulation of key enzyme steps in the shikimate pathway and
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
... present in A. methanolica. DAHP synthase 1 (DS1) is a 160 kDa enzyme associated non-covalently with a dimeric CM protein, thus forming a heteromeric two-enzyme complex. The two enzyme activities can be separated by Q-Sepharose anion-exchange chromatography, yielding a dimeric CM protein with a fivef ...
Alpha and beta subunits of pyruvate dehydrogenase E1
... recognisable mitochondrion, but recently have been shown to have evolved from fungi and to possess heat shock protein genes derived from mitochondria. These findings make it clear that microsporidian ancestors were mitochondrial, yet it remains unknown whether they still contain the organelle, and i ...
... recognisable mitochondrion, but recently have been shown to have evolved from fungi and to possess heat shock protein genes derived from mitochondria. These findings make it clear that microsporidian ancestors were mitochondrial, yet it remains unknown whether they still contain the organelle, and i ...
RECOMBINANT-DNA METHODOLOGY
... Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place. It’s also a useful way to name pieces of DNA. For example, a piece of DNA that is cut from a bigger piece of DNA is often named by size and given a surname that corresponds to the two ...
... Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place. It’s also a useful way to name pieces of DNA. For example, a piece of DNA that is cut from a bigger piece of DNA is often named by size and given a surname that corresponds to the two ...
Expression and purification of four different rhizobial acyl carrier
... oligonucleotides were used in a PCR to amplify and subsequently clone that part of the gene encoding the aminoterminal half of the AcpP of S. meliloti. These oligonucleotides were deduced from the amino acid sequence of different ACPs, among them the sequence of the AcpP of S. meliloti 1021 (Platt e ...
... oligonucleotides were used in a PCR to amplify and subsequently clone that part of the gene encoding the aminoterminal half of the AcpP of S. meliloti. These oligonucleotides were deduced from the amino acid sequence of different ACPs, among them the sequence of the AcpP of S. meliloti 1021 (Platt e ...
Transfer of genetic material between the chloroplast and nucleus
... nuclear genome data reported that the plant nuclear genome is in equilibrium between frequent integration and rapid elimination of the chloroplast genome and that the pericentromeric regions play a significant role in facilitating the chloroplast–nuclear DNA flux. This equilibrium between integratio ...
... nuclear genome data reported that the plant nuclear genome is in equilibrium between frequent integration and rapid elimination of the chloroplast genome and that the pericentromeric regions play a significant role in facilitating the chloroplast–nuclear DNA flux. This equilibrium between integratio ...
Expression of phosphofructokinase in Neisseria meningitidis
... during the evolution of the species this activity was lost (Bapteste et al., 2003), the question of why N. meningitidis lost PFK activity remains. In particular, since glucose is present within nasopharyngeal tissue (Exley et al., 2005), the presence of PFK can yield one additional ATP per unit of s ...
... during the evolution of the species this activity was lost (Bapteste et al., 2003), the question of why N. meningitidis lost PFK activity remains. In particular, since glucose is present within nasopharyngeal tissue (Exley et al., 2005), the presence of PFK can yield one additional ATP per unit of s ...
Regulation of nitrogen metabolism in gram
... TnrA/GlnR site upstream of orthologous genes in at least two genomes. Another 11 operons lacked the conserved TnrA-binding site, but their products were involved in nitrogen assimilation (Fig. 3). In all genomes analyzed, the conserved TnrAbinding site was observed upstream of the nrgAB operon. In B ...
... TnrA/GlnR site upstream of orthologous genes in at least two genomes. Another 11 operons lacked the conserved TnrA-binding site, but their products were involved in nitrogen assimilation (Fig. 3). In all genomes analyzed, the conserved TnrAbinding site was observed upstream of the nrgAB operon. In B ...
Chromosomal rearrangements and protein globularity changes in
... PPE53 and PPE24) were found in at least four of the strains. The variants in eight of these genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis ...
... PPE53 and PPE24) were found in at least four of the strains. The variants in eight of these genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis ...
BAK1 Gene Variation: the doubts remain
... codon, CGC, is really problematic, for in refseq NM_001188.3 it is supposed to code for ARG, while in BAK1 on chromosome 20 the codon is CAC which codes for HIS. It occurs that in their Table 2 (rows 4 and 5, non-diseased AA and AAA) the codon CAA appears, which in fact codes for GLN. The 52nd amino ...
... codon, CGC, is really problematic, for in refseq NM_001188.3 it is supposed to code for ARG, while in BAK1 on chromosome 20 the codon is CAC which codes for HIS. It occurs that in their Table 2 (rows 4 and 5, non-diseased AA and AAA) the codon CAA appears, which in fact codes for GLN. The 52nd amino ...
molecular phylogeny of the haplosporidia based on
... The SSU rRNA genes for most of the haplosporidian species were amplified in the polymerase chain reaction (PCR) using ‘‘universal’’ eukaryotic primers (Medlin et al., 1988) and conditions previously described (Flores et al., 1996). Internal SSU rRNA gene primers Urocomp280SSU (Reece and Stokes, 2003 ...
... The SSU rRNA genes for most of the haplosporidian species were amplified in the polymerase chain reaction (PCR) using ‘‘universal’’ eukaryotic primers (Medlin et al., 1988) and conditions previously described (Flores et al., 1996). Internal SSU rRNA gene primers Urocomp280SSU (Reece and Stokes, 2003 ...
Tissue-specific codon usage and the expression of
... patterns from only two studies, we limited ourselves to fewer data than are available in large compilations of many expression studies. On the other hand, the expression data we have used are comparable (both studies used the GeneChip HuGeneFL microarray), and they provide a consistent, unbiased met ...
... patterns from only two studies, we limited ourselves to fewer data than are available in large compilations of many expression studies. On the other hand, the expression data we have used are comparable (both studies used the GeneChip HuGeneFL microarray), and they provide a consistent, unbiased met ...
Translation Tutorial
... A process called transcription starts in the nucleus, where an enzyme called RNA polymerase splits the DNA molecule. Next, free floating mRNA nucleotides bond to the open DNA molecule. next Once finished, the mRNA breaks away and exits the nucleus. The mRNA will then join a ribosome. Now, the proces ...
... A process called transcription starts in the nucleus, where an enzyme called RNA polymerase splits the DNA molecule. Next, free floating mRNA nucleotides bond to the open DNA molecule. next Once finished, the mRNA breaks away and exits the nucleus. The mRNA will then join a ribosome. Now, the proces ...
A Molecular Basis for Multiple Herbicide Resistance in Black
... C120V mutant independently expressed as Strep II tag fusions. • In vitro inhibition of activity towards CDNB. ...
... C120V mutant independently expressed as Strep II tag fusions. • In vitro inhibition of activity towards CDNB. ...
Nucleic Acids
... the antiparallel strand must be thymine. Similarly, if the purine in one strand is guanine, its complement in the antiparallel strand must be cytosine. A significant feature of Watson and Crick’s model is that no other base pairing is consistent with the observed thickness of a DNA molecule. A pair o ...
... the antiparallel strand must be thymine. Similarly, if the purine in one strand is guanine, its complement in the antiparallel strand must be cytosine. A significant feature of Watson and Crick’s model is that no other base pairing is consistent with the observed thickness of a DNA molecule. A pair o ...