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Supplementary Methods (doc 30K)
Supplementary Methods (doc 30K)

... Supplemental methods DNA Constructs and reagents The NF-кB p65 and p50 expression plasmids were used to produce full-length p65 and p50 protein. It was made by cloning PCR products into the HindIII and EcoRV sites of pFlag-CMV-2 expression vector as described before. (Hertlein E et al. 2005). The NF ...
DNA, RNA and Protein
DNA, RNA and Protein

... Question: How many electrons does carbon need to fill its outer energy level? Answer: Therefore, each carbon atom can make ____ covalent bonds with other types of atoms or additional carbons. ...
Chapter 4 • Lesson 21
Chapter 4 • Lesson 21

... The ribosome's job is to assemble amino acids into polypeptide chains, which are in turn combined into proteins. Ribosomal RNA (rRNA) is RNA that makes up part of the structure of a ribosome and assists in translation. During translation, up to two tRNA molecules are connected to the mRNA at a time ...
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No Slide Title

... Problem 15.5 Consider a hypothetical regulatory scheme in which citrulline induces the production of urea cycle enzymes. Four genes (citA, citB, citC, citD) affecting the activity or regulation of the enzymes were analyzed by assaying the wild-type and mutant strains for argininosuccinate lyase act ...
Post-transcriptional gene control
Post-transcriptional gene control

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nucleus

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DNA and Protein Synthesis
DNA and Protein Synthesis

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...  phosphorylation of glucose to glucose phosphate, using ATP  production of triose phosphate  oxidation of triose phosphate to pyruvate with a net gain of ATP and reduced NAD. If respiration is only anaerobic, pyruvate can be converted to ethanol or lactate using reduced NAD. The oxidised NAD prod ...
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video slide - Wild about Bio

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AQA Biology - Centre of the Cell

... • the amino acid sequence of a polypeptide • a functional RNA (including ribosomal RNA and tRNAs). A gene occupies a fixed position, called a locus, on a particular DNA molecule. A sequence of three DNA bases, called a triplet, codes for a specific amino acid. The genetic code is universal, non-over ...
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DNA REVIEW SHEET (answer in COMPLETE sentences on another

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Chapter 20 Notes: DNA Technology

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Chapter 15: Genetic Engineering

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DNA - hdueck

... Ribonucleic Acid Types (p 288-295) There are several types. We will focus on the main 3 types: rRNA: large, makes up structure of ribosomes. - Large globular structure, forms structure with proteins to form ribosome tRNA: smaller, contains amino acid to match code of mRNA. Compact 3-D structure mRN ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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