• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
DNA
DNA

... All living things have DNA •We recycle the DNA in foods we eat. It is broken down into its basic parts and reused, like legos. •DNA is easy to extract from non-cooked foods ...
Changes in DNA can produce Variation
Changes in DNA can produce Variation

Biotechnology
Biotechnology

... random match becomes smaller. ...
Human Genetics and Genetic Technology Test Review Jeopardy
Human Genetics and Genetic Technology Test Review Jeopardy

... The thing that transplant DNA is placed into to be delivered to the new host ...
Biotechnology and Genetic Engineering
Biotechnology and Genetic Engineering

... Insert a foreign gene into a host Plasmid, then into the bacterial cell – transformation Transgenic organisms Recombinant DNA  Goal – To produce many copies ( clones) of a particular gene (PCR)  Genetic marker – tags gene of interest – to identify the presence of a gene ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 6. Gene silencing can be used for turning off a gene 7. Meristem culture is used to eliminate virus in tissue culture 8. Barbara McClintok discovered jumping genes 9. Particle gun bombardment technique cannot be used for gene transfer in plants 10. Haploid set of chromosome (n) of an organism is ter ...
Practice Question for Replication, Genetics and Biotechnology
Practice Question for Replication, Genetics and Biotechnology

... 28. A trait that expresses itself as a heterozygote is ______________ (dominant or recessive). 29. Sex linked traits are found on the _____________________ chromosome. 30. People who have one copy of an allele for a recessive disorder, but do not exhibit symptoms are called _________ 31. Is blood ty ...
Genetics 1. What do the letters DNA stand for? 2. Two scientists are
Genetics 1. What do the letters DNA stand for? 2. Two scientists are

... _______________________ and the base _______________________ pairs with ___________________ ____ in the formation of the DNA molecule.This is called complementary base pairs. Thus one strand of DNA is complementary to the other strand (opposite/matching). ...
Site-specific recombination mechanisms exploit DNA
Site-specific recombination mechanisms exploit DNA

... Through a series of elegant genetic experiments in the early eighties Piet van de Putte (Laboratory of Molecular Genetics, Leiden University) determined that bacteriophage (Mu) changes its host range through expression of different tail fibers by changing the orientation of a specific DNA segment, t ...
File - Cowan Science
File - Cowan Science

... 1. Scientists use knowledge of DNA to change DNA molecules ...
Key for Practice Exam 4
Key for Practice Exam 4

lecture0
lecture0

... Signal Transduction Pathway ...
國立嘉義大學九十一學年度
國立嘉義大學九十一學年度

... 19.A DNA sequence (mobile genetic element) that can insert randomly into a chromosome, exit the site, and relocate at another chromosomal site. 20.A process that uses living organisms to remove contaminants, pollutants, or unwanted substances from soil or water. Part B. Each question counts 10 point ...
HomeworkCh7
HomeworkCh7

... a. What is the role of sigma factors in Bacterial RNA synthesis from DNA. b. What is the role of transcription factors in Archaea and Eukarya? Hint. Same as sigma factors in bacteria. c. What is a promotor? d. What are the three main phases of RNA synthesis? e. Can more than one copy of the gene be ...
Feb 21 Bacteria, DNA Technology, and Cell Communication
Feb 21 Bacteria, DNA Technology, and Cell Communication

... Long distance communication in the body hormonal signaling Signal transduction- reception, transduction, response Steroid hormones G-protein-linked plasma membrane receptors Plasma membrane tyrosine kinases Plasma membrane ion-gated channel receptors Signal transduction cascades Second messengers S ...
Big
Big

... Recombinant DNA is the product of combining two (or more) separate sources of DNA – Usually, the insertion of a foreign gene into an organism. • Human insulin in E. coli for drug production • Herbicide resistance into crop plants. ...
What`s the Big Deal About DNA?
What`s the Big Deal About DNA?

... 2. Explain what the human genome is. Why do scientists want to study it? ...
E:Med - uni-freiburg.de
E:Med - uni-freiburg.de

... Predicted affinity of EBF1 correlates with change in DNA methylation ...
Changes in DNA can produce Variation
Changes in DNA can produce Variation

Biology Ch. 12 Vocab
Biology Ch. 12 Vocab

... ribosomes 19. expressed sequence of DNA; codes for a protein ...
Working with Data Recombinant DNA
Working with Data Recombinant DNA

... functional recombinant bacterial plasmids can be constructed in the laboratory. Specifically, the scientists used restriction enzymes to cut two E. coli plasmids containing a resistance gene for either kanamycin or tetracycline. The plasmids were joined together using the enzyme DNA ligase, and the ...
PowerPoint
PowerPoint

... material in an organism is the genome. • 2. Locating and recording the site of specific genes within the chromosomes is gene mapping.  Selected portions of DNA containing the desired gene are cut with a restriction enzyme. ...
Nair.Amritha.Ashok, Belligere.Kempegowda.Spoorthi, Ashcheulova
Nair.Amritha.Ashok, Belligere.Kempegowda.Spoorthi, Ashcheulova

... makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. The techniques involve sophisticated manipulations of genetic material and other biologically important chemicals. Genetically modified organisms (GMOs) can be defined as org ...
Transformation and Cloning
Transformation and Cloning

... • Some present in multiple copies. • Independently replicate inside bacteria. ...
Reading Study Guide B
Reading Study Guide B

... Describe the DNA transcription process by completing each sentence. During transcription, DNA is used to make _______________________________________. Only _________________________________________________________ are transcribed. Many copies of RNA can be made from _________________________________ ...
< 1 ... 627 628 629 630 631 632 633 634 635 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report