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Word Picture Definition Gene mRNA Base Uracil Ribosome tRNA
Word Picture Definition Gene mRNA Base Uracil Ribosome tRNA

... c) 3rd sentence = Explain HOW your evidence works d) 4th sentence = Summarize answer -OR- Second piece of evidence A) When cells make proteins, why do they make a copy of mRNA to send out of the nucleus to the ribosome? Why don’t they just send a piece of the original DNA, to make sure there can’t b ...
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DNA replication is molecular mechanism of

... 22. There exist a total of 20 amino acids that our bodies use to make polypeptides with. Remember a protein is made up of one or more than one _____________________. Today we know that there are ____ codons, but only _____ code for amino acids. An amino acid may be coded for by more than one codon, ...
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Chapter 17 - Denton ISD

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Uses for transgenic organisms (also called GMO`s or genetically

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Studying the Human Genome

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DNA …… solving the puzzle of life

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... A Codon is a triplet of base pairs. Each codon corresponds to one of twenty Amino acids -- it’s the amino acids that are the building-blocks of proteins, which do the work of the cell. A gene is a sequence of codons. Each gene corresponds to a particular protein that is used by the cell to do its wo ...
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Recitation 10 Solutions

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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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