Biotechnological Methods and Products
... Selecting the Vector Selecting the Host Cell Selecting the DNA Delivery Method Constructing the Recombinant DNA ...
... Selecting the Vector Selecting the Host Cell Selecting the DNA Delivery Method Constructing the Recombinant DNA ...
Chapter 20: DNA Technology and Genomics
... Cells containing recombinant plasmid are identified by their ability to grow in presence of the antibiotic and by their white color. Blue colonies contain plasmids hat resealed and thus have a functioning lacZ gene. Identify clones carrying gene of interest with nucleic acid probe or by protein prod ...
... Cells containing recombinant plasmid are identified by their ability to grow in presence of the antibiotic and by their white color. Blue colonies contain plasmids hat resealed and thus have a functioning lacZ gene. Identify clones carrying gene of interest with nucleic acid probe or by protein prod ...
Chapter 12 - Biotechnology
... Viruses • Viruses are the vectors of choice for animal cells. • They can accept larger amounts of DNA than plasmids. • When the virus reproduces within the animal cell, it also reproduces the foreign gene that it carries. The gene is therefore cloned. • The DNA of some retroviruses becomes integrate ...
... Viruses • Viruses are the vectors of choice for animal cells. • They can accept larger amounts of DNA than plasmids. • When the virus reproduces within the animal cell, it also reproduces the foreign gene that it carries. The gene is therefore cloned. • The DNA of some retroviruses becomes integrate ...
Chapter 12 - Biotechnology
... Viruses • Viruses are the vectors of choice for animal cells. • They can accept larger amounts of DNA than plasmids. • When the virus reproduces within the animal cell, it also reproduces the foreign gene that it carries. The gene is therefore cloned. • The DNA of some retroviruses becomes integrate ...
... Viruses • Viruses are the vectors of choice for animal cells. • They can accept larger amounts of DNA than plasmids. • When the virus reproduces within the animal cell, it also reproduces the foreign gene that it carries. The gene is therefore cloned. • The DNA of some retroviruses becomes integrate ...
MUTATIONS
... DNA just happen. Our DNA can change without warning, which changes the genes and how they behave. Factors that cause changes in our DNA: Errors when DNA is copied for new cells Environmental factors change DNA (nicotine, sunlight, x-rays, chemicals Mutations are inherited from the parents ...
... DNA just happen. Our DNA can change without warning, which changes the genes and how they behave. Factors that cause changes in our DNA: Errors when DNA is copied for new cells Environmental factors change DNA (nicotine, sunlight, x-rays, chemicals Mutations are inherited from the parents ...
ch 20 biotech clicker questions
... You have a restriction enzyme that makes a blunt cut between an A and a T. What will the size of the DNA fragments be after the following DNA molecule is cut with this restriction enzyme: 5′-TTGTTCGGATCCCGTAGG-3′? a) one 9-bp fragment, one 6-bp fragment, and one 3bp fragment b) one 15-bp fragment a ...
... You have a restriction enzyme that makes a blunt cut between an A and a T. What will the size of the DNA fragments be after the following DNA molecule is cut with this restriction enzyme: 5′-TTGTTCGGATCCCGTAGG-3′? a) one 9-bp fragment, one 6-bp fragment, and one 3bp fragment b) one 15-bp fragment a ...
The Good, the bad and the ugly of Genetic Engineering
... bacteria 3.Bacteria then MAKE human insulin even though they have no use for it! 4.We extract the insulin from bacteria and use it in injections ...
... bacteria 3.Bacteria then MAKE human insulin even though they have no use for it! 4.We extract the insulin from bacteria and use it in injections ...
Genetic engineering
... (1.) bacterial cells that are unable to synthesize insulin (2.) human cells that are able to synthesize antibodies (3.) bacterial cells that are able to synthesize insulin (4.) human cells that are unable to resist antibiotics 2. What is this process an example of? ...
... (1.) bacterial cells that are unable to synthesize insulin (2.) human cells that are able to synthesize antibodies (3.) bacterial cells that are able to synthesize insulin (4.) human cells that are unable to resist antibiotics 2. What is this process an example of? ...
Bacterial genetics - Comenius University
... resistance transfer factor RTF) - mediate their own transfer - conjugation • Smaller plasmids - not conjugative - do not encode transfer protein - sedentary - do not transfer ...
... resistance transfer factor RTF) - mediate their own transfer - conjugation • Smaller plasmids - not conjugative - do not encode transfer protein - sedentary - do not transfer ...
Bacterial genetics
... resistance transfer factor RTF) - mediate their own transfer - conjugation • Smaller plasmids - not conjugative - do not encode transfer protein - sedentary - do not transfer ...
... resistance transfer factor RTF) - mediate their own transfer - conjugation • Smaller plasmids - not conjugative - do not encode transfer protein - sedentary - do not transfer ...
Compendium 11 Learning Outcomes • Describe the structure and
... • Mitosis - The series of events that lead to the production of two cells by division of a mother cell into two daughter cells • Nitrogenous base - A nitrogen containing molecule that has the same chemical properties as a base and bonds nucleic acids together • Ribonucleic acid (RNA) - A nucleic aci ...
... • Mitosis - The series of events that lead to the production of two cells by division of a mother cell into two daughter cells • Nitrogenous base - A nitrogen containing molecule that has the same chemical properties as a base and bonds nucleic acids together • Ribonucleic acid (RNA) - A nucleic aci ...
ap-biology-big-idea-3-review-answers
... 3. What does it mean when we say that DNA replication occurs bidirectionally (reference leading versus lagging strands in your answer – and what is the name of the fragment created in the lagging strand)? Leading strand is processed from 3’ to 5’ away from the fork while Okazaki fragments are made o ...
... 3. What does it mean when we say that DNA replication occurs bidirectionally (reference leading versus lagging strands in your answer – and what is the name of the fragment created in the lagging strand)? Leading strand is processed from 3’ to 5’ away from the fork while Okazaki fragments are made o ...
Genetic Engineering
... DNA at specific nucleotide sequences called “restriction sites” Used to "cut and splice" DNA Obtained from bacteria Ex. EcoRI and Hind III ...
... DNA at specific nucleotide sequences called “restriction sites” Used to "cut and splice" DNA Obtained from bacteria Ex. EcoRI and Hind III ...
No Slide Title
... Nothing will happen with the first pregnancy. The mother’s antibodies can attack the 2nd baby causing severe problems or death for the baby. A shot is given after the first pregnancy to inhibit antibody production in the negative mother. ...
... Nothing will happen with the first pregnancy. The mother’s antibodies can attack the 2nd baby causing severe problems or death for the baby. A shot is given after the first pregnancy to inhibit antibody production in the negative mother. ...
Trends in Biotechnology
... create double-stranded DNA from RNA template, so that the DNA can integrate into the host cell’s genome. It needs to infect actively dividing cells. Adenovirus — a double-stranded DNA virus that can infect many types of host cells with high efficiency, with a low chance for causing disease. It does ...
... create double-stranded DNA from RNA template, so that the DNA can integrate into the host cell’s genome. It needs to infect actively dividing cells. Adenovirus — a double-stranded DNA virus that can infect many types of host cells with high efficiency, with a low chance for causing disease. It does ...
DNA Notesheet
... _ _ 2. L: LOCATE evidence from the text (notes) to support your answer. _ _ _ 3. A: ADD additional evidence OR your own ANALYSIS (how does your evidence support your answer?) _ _ _ _ _ 4. M: MAKE a meaningful conclusion or connection: _ _ _ _ ...
... _ _ 2. L: LOCATE evidence from the text (notes) to support your answer. _ _ _ 3. A: ADD additional evidence OR your own ANALYSIS (how does your evidence support your answer?) _ _ _ _ _ 4. M: MAKE a meaningful conclusion or connection: _ _ _ _ ...
Unit 2 – Genetics Content Map
... What controls the rate of cellular reproduction? What are the advantages of asexual reproduction? What are the advantages of sexual reproduction? ...
... What controls the rate of cellular reproduction? What are the advantages of asexual reproduction? What are the advantages of sexual reproduction? ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.