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BIO113H - willisworldbio
BIO113H - willisworldbio

... After transformation the cells are treated with and ___________. Only those cells that have been transformed ______, because only they carry a ________ gene. ...
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13 Packet

... Concept 13.2 Biologists can engineer bacteria to make useful products. (pp. 268–273) Many bacteria contain plasmids, which are small, circular DNA molecules that are separate from the much larger bacterial chromosome. Biologists use plasmids to move genes into bacteria. A restriction enzyme “cuts” a ...
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...  Amino-acid sequence detection via hybridization with probes o Reverse transcriptase-polymerase chain reaction  cDNA synthesis from mRNA present at time of interest during metabolic pathway / developmental stages  PRC amplification using gene specific primers  Gel electrophoresis indicates prese ...
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Chapter9 (and Section 8-4): Genetic Engineering

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... • Integration of retroviral vectors only in host cells replicating their DNA. • Many vectors illicit an immune response. • Insertion into genes can inactivate them. • Vectors can carry a limited amount of DNA. • New generation vectors are addressing these problems. ...
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Grade 10 – Reproduction and Genetics

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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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