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DNA Structure and Replication
DNA Structure and Replication

... ! Priming (DNA synthesis needs a primer): RNA "primase" makes RNA; DNA added ! Antiparallel templates: Okazaki fragments of new DNA on one strand (for a short time) ...
Document
Document

... 2. cells from fetus or surrounding fluid - grow to many cells in nutrients = cell culture 3. check for DNA pattern associated with the disorder B. Gene Therapy - insertion of normal genes into the human cells to correct genetic disorders Ex: first trials on patients with cystic fibrosis - hopefully ...
university of oslo
university of oslo

... Example: the use of glucose and lactose by E. coli. Glucose indirectly prevents binding of the catabolite activator protein (CAP) to the DNA upstream of the lac operon by causing dephosphorylation of protein IIAGlc. Dephosphorylated IIAGlc inhibits adenylate cyclase, the enzyme that catalyzes the fo ...
Slide 1
Slide 1

... • Two DNA molecules may recombine segments of their molecule in a process called crossing over. • This is a relatively common event between chromosome copies in eukaryotes during meiosis. (Note the example here.) • Prokaryote chromosomes, viral DNA, and smaller fragments of “foreign” DNA may recombi ...
Exam V2002 - English
Exam V2002 - English

... Example: the use of glucose and lactose by E. coli. Glucose indirectly prevents binding of the catabolite activator protein (CAP) to the DNA upstream of the lac operon by causing dephosphorylation of protein IIAGlc. Dephosphorylated IIAGlc inhibits adenylate cyclase, the enzyme that catalyzes the fo ...
DNA/RNA
DNA/RNA

... 9 RNA nucleotides bind to the template strands of the DNA ...
BP 32: Posters - DNA/RNA - DPG
BP 32: Posters - DNA/RNA - DPG

... surface-bound DNA brushes into arbitrarily shaped DNA bundles. DNA molecules of 1 micron length (3 kbp) were immobilized on lines as thin as 100 nm in width, before condensation was induced by addition of spermidine. Starting at a nucleation site, DNA condensates grew via an inverted domino effect b ...
Worksheet for Biology 1107 Biological Molecules: Structure and
Worksheet for Biology 1107 Biological Molecules: Structure and

... in lab: answer the following questions, and turn them in to your instructor. 1. The two groups of monosaccharides that are most important to biologists have how many carbons? ...
Guidelines and Assignments
Guidelines and Assignments

... 1. (MT1) A. How is the 5-mC distributed within the human genome? B. Do all human genes have CpG island at their promoters? C. How bisulfite treatment may affect the CpG methylation status? D. What methods can be used to detect the methylation status of DNA? Please describe at least four different me ...
2nd Semester Biology Tournament - d
2nd Semester Biology Tournament - d

... 43. atom, molecule, organelle, cell, tissues, organs, organ systems, organism, population, community, ecosystem. 44. Natural selection is the survival of the individuals best suited to their environment and the passing on of these beneficial traits to their offspring. 45. Evolution is the change in ...
Randy Carroll
Randy Carroll

... RNA. During transcription, RNA polymerises binds to the promoter of a specific gene. The DNA makes a copy of those using RNA nucleotides. 2. The structure of RNA is a single helix and that thiamine is rarely part of the RNA molecules. 3. Messenger RNA: consists of RNA nucleotides and carries genetic ...
Biology
Biology

... Directional Selection: This happens when one of the “extremes” is favored in an environment over the other extreme or the old ...
Review questions to go with the powerpoint
Review questions to go with the powerpoint

... 54.A karyotype shows all of your ______________ and can detect _______________ disorders. 55.The Human Genome Project ______________ all of human ______. This information has been used for ________ therapy. 56.DNA put together from 2 different species is called _________________ DNA. 57.A ________ i ...
Unit 4: Genetic Engineering and Gene Expression
Unit 4: Genetic Engineering and Gene Expression

... 17. What are some ways in which the GFP gene is being used in medical research? (Think back to the article, “Green pig gives birth to glowing piglets”) Spread of cancer cells, organ transplants GMO Questions 18. How is recombinant DNA technology used in the agricultural industry? How can we test to ...
NOVA Online Cancer Tutorial
NOVA Online Cancer Tutorial

Topics that we have covered
Topics that we have covered

Overview of the Recombinant DNA technology- the plasmid vector pUC19
Overview of the Recombinant DNA technology- the plasmid vector pUC19

Transcribe and Translate a Gene
Transcribe and Translate a Gene

... BI4. a. Students know the general pathway by which ribosomes synthesize proteins, using tRNAs to translate genetic information in mRNA.  BI5. a. Students know the general structures and functions of DNA, RNA, and protein. .Objectives: SWBAT…  Explain the genetic factors that influence the way we l ...
File
File

... DNA Fingerprinting Activity Introduction: DNA fingerprinting relies on the fact that the DNA code is universal for all living things and that there are differences between individuals within that code. Because human DNA is very similar to every other human’s DNA, DNA fingerprinting primarily focuses ...
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day2

... E. coli = 4,639,221 bp, 4.6 Mb Human = ~~ 3,300 Mb • Contain a small amount of noncoding DNA ...
Document
Document

... This image was taken shortly after DNA a replication but before the prophase. It is composed of two daughter chromatids joined at the centromere. The chromosome is super coiled by a factor around x16,000. The DNA molecule is about 1.8m long but is located in the nucleus which is only 10um in diamete ...
Genetic Engineering - Biology Class With Mrs. Caskey
Genetic Engineering - Biology Class With Mrs. Caskey

... List one behavior or physical characteristic of that species that was targeted through selective breeding. ...
Replication of DNA - Biology-RHS
Replication of DNA - Biology-RHS

... DNA separate, serve as templates, and produce DNA molecules that have one strand parental DNA and one strand of new DNA  Semi-conservative replication occurs in 3 main ...
DNA Bank Acquisitions Policy
DNA Bank Acquisitions Policy

... The DNA Bank of The New York Botanical Garden (NYBG) serves as the repository for samples of frozen tissue and genomic DNA for research conducted in the Garden’s molecular systematics and genomics laboratories by scientists, graduate students, visiting scholars, and interns. In support of the Garden ...
Ch. 11
Ch. 11

... A. Genes and Proteins – the sequences of nucleotides in DNA contain information to code for essential proteins such as enzymes and filaments that compose body tissue. Proteins are strands of ______________ ____________ coded for by DNA. B. RNA (ribonucleic acid)– differs from DNA in 3 ways. a. RNA i ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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