BIOTECHNOLOGY AND GENETIC ENGINEERING
... What is the difference between gene therapy and genetic engineering? Difference between a hybrid and chimera? Steps of genetic engineering? The Hind R1 restriction enzyme is used to slice DNA at the GAATTC between the G and A. Illustrate how this enzyme would precisely cut the ...
... What is the difference between gene therapy and genetic engineering? Difference between a hybrid and chimera? Steps of genetic engineering? The Hind R1 restriction enzyme is used to slice DNA at the GAATTC between the G and A. Illustrate how this enzyme would precisely cut the ...
Learning Targets
... explain the steps of DNA replication in cells and hereditary coding. 11. What are the roles of the DNA, mRNA, tRNA, rRNA, and ribosomes in protein synthesis? 12. What are the steps of the transcription phase of protein synthesis? 13. What are the steps of the translation phase of protein synthesis, ...
... explain the steps of DNA replication in cells and hereditary coding. 11. What are the roles of the DNA, mRNA, tRNA, rRNA, and ribosomes in protein synthesis? 12. What are the steps of the transcription phase of protein synthesis? 13. What are the steps of the translation phase of protein synthesis, ...
Chapter 12 powerpoint
... Enzymes in Replication • Enzymes unwind the two strands • DNA polymerase attaches complementary nucleotides • DNA ligase fills in gaps ...
... Enzymes in Replication • Enzymes unwind the two strands • DNA polymerase attaches complementary nucleotides • DNA ligase fills in gaps ...
March10NaturalSelection
... expressed as phenotype A string of molecular symbols AACCGGTAGTCTATGCTAGTGGGGTTTTAATAAT… is turned into a protein that makes your hair brown, curly or fall out when you’re 30 ...
... expressed as phenotype A string of molecular symbols AACCGGTAGTCTATGCTAGTGGGGTTTTAATAAT… is turned into a protein that makes your hair brown, curly or fall out when you’re 30 ...
introductory slides
... Excision of “SWR peptide” from Lipin gene 1. Cut dLipin gene with restriction enzymes on either side of conserved sequence and remove excised ...
... Excision of “SWR peptide” from Lipin gene 1. Cut dLipin gene with restriction enzymes on either side of conserved sequence and remove excised ...
You Light Up My Life
... • DNA is two nucleotide strands held together by hydrogen bonds • Hydrogen bonds between two strands are easily broken ...
... • DNA is two nucleotide strands held together by hydrogen bonds • Hydrogen bonds between two strands are easily broken ...
Unit 1: Cells, Cell Reproduction, and Development
... o What is the probability that these parents will create this child? What relatives are considered 1, and how many genes do you share in common with these relatives? What about 2 and 3? What does a heritability number mean? What does a concordance study look at? ...
... o What is the probability that these parents will create this child? What relatives are considered 1, and how many genes do you share in common with these relatives? What about 2 and 3? What does a heritability number mean? What does a concordance study look at? ...
E. Coli - mrkeay
... • Enzyme used to join the cut strands of DNA together (best for sticky ends) • T4 DNA ligase (from T4 bacteriophage virus) works better for blunt ends ...
... • Enzyme used to join the cut strands of DNA together (best for sticky ends) • T4 DNA ligase (from T4 bacteriophage virus) works better for blunt ends ...
Document
... acid that the codon codes 2. Does not cause alteration on the amino acid that the codon codes 3. Alters codon in the way that it becomes stop-codon for protein synthesis ...
... acid that the codon codes 2. Does not cause alteration on the amino acid that the codon codes 3. Alters codon in the way that it becomes stop-codon for protein synthesis ...
PDF file of the lecture on "Gene Transfer"
... How to make cells competent? • E.coli does not develop competence in normal growth; however, competence can be induced in the lab: – Chemically: by chilling the cells at 4°C a[er treaAng with CaCl2 ...
... How to make cells competent? • E.coli does not develop competence in normal growth; however, competence can be induced in the lab: – Chemically: by chilling the cells at 4°C a[er treaAng with CaCl2 ...
Timeline of Genetic Engineering
... Gene Therapy 1. Process of changing a gene to treat a medical disease or disorder. 2. Absent or faulty gene is replaced by a normal, working gene. 3. This process allows the body to make the protein or enzyme it needs, which eliminates the cause of the disorder. ...
... Gene Therapy 1. Process of changing a gene to treat a medical disease or disorder. 2. Absent or faulty gene is replaced by a normal, working gene. 3. This process allows the body to make the protein or enzyme it needs, which eliminates the cause of the disorder. ...
Presentation
... genes express themselves only during specific periods in the life of an organism and may be recessive genes that show themselves only when in the homozygous state. Characteristics that are acquired during the life of the individual and are not determined by genes cannot be raw material for natural s ...
... genes express themselves only during specific periods in the life of an organism and may be recessive genes that show themselves only when in the homozygous state. Characteristics that are acquired during the life of the individual and are not determined by genes cannot be raw material for natural s ...
Pierce chapter 10
... – Studied pus (contains white blood cells) – Isolated nuclear material • Slightly acidic, high phosphorous content • Consisted of DNA and protein – Called in “nuclein” – later renamed nucleic acid ...
... – Studied pus (contains white blood cells) – Isolated nuclear material • Slightly acidic, high phosphorous content • Consisted of DNA and protein – Called in “nuclein” – later renamed nucleic acid ...
Bio 102 Practice Problems
... D. TACGTA E. CCTAGG 6. To change a simple cloning vector (or plasmid) into an expression vector, we would need to add A. a site that can be cut by a restriction enzyme. B. an origin of replication. C. one or more introns. D. a -10 and a -35 box. E. a gene encoding resistance to an antibiotic. ...
... D. TACGTA E. CCTAGG 6. To change a simple cloning vector (or plasmid) into an expression vector, we would need to add A. a site that can be cut by a restriction enzyme. B. an origin of replication. C. one or more introns. D. a -10 and a -35 box. E. a gene encoding resistance to an antibiotic. ...
Fifth Lecture
... Effects of ionizing radiation on DNA &RNA • Active enzymatic repair processes exist for the repair of both DNA base damage and strand breaks. • In many cases breaks in the double-strand DNA can be repaired by the enzymes, DNA polymerase, and DNA ligase. • The repair of double strand breaks involves ...
... Effects of ionizing radiation on DNA &RNA • Active enzymatic repair processes exist for the repair of both DNA base damage and strand breaks. • In many cases breaks in the double-strand DNA can be repaired by the enzymes, DNA polymerase, and DNA ligase. • The repair of double strand breaks involves ...
You Asked for it…..
... Remember, genes are made of DNA and are in the nucleus Genes (DNA) contain the instruction for making a protein In transcription, DNA is used to make mRNA in the nucleus mRNA then leaves the nucleus and goes to the ribosome In translation, tRNA then brings amino acids in the proper order to make the ...
... Remember, genes are made of DNA and are in the nucleus Genes (DNA) contain the instruction for making a protein In transcription, DNA is used to make mRNA in the nucleus mRNA then leaves the nucleus and goes to the ribosome In translation, tRNA then brings amino acids in the proper order to make the ...
Abstract - IJCMAAS
... molecular biology tests. Most of the laboratories are using kit based DNA extraction methods, which is expensive. We compared the kit based DNA extraction with a conventional technique of DNA extraction based on the Perchlorate technique. Material and Method: DNA was extracted on 60 samples by the k ...
... molecular biology tests. Most of the laboratories are using kit based DNA extraction methods, which is expensive. We compared the kit based DNA extraction with a conventional technique of DNA extraction based on the Perchlorate technique. Material and Method: DNA was extracted on 60 samples by the k ...
CREDGREW power point
... “producer”; examples are plants and algae • Heterotroph = get food from other organisms; also known as a “consumer” ...
... “producer”; examples are plants and algae • Heterotroph = get food from other organisms; also known as a “consumer” ...
A2 5.2.3 Genetic Engineering
... • Used enzyme reverse transcriptase to make a complimentary DNA strand (the same as the template one would have been) • DNA polymerase and free nucleotides added • Copies made called cDNA and unpaired nucleotides added to make sticky ends • Plasmids cut with restriction enzyme and mixed with cDNA, t ...
... • Used enzyme reverse transcriptase to make a complimentary DNA strand (the same as the template one would have been) • DNA polymerase and free nucleotides added • Copies made called cDNA and unpaired nucleotides added to make sticky ends • Plasmids cut with restriction enzyme and mixed with cDNA, t ...
the VECTOR (gene carrier)
... BIOTECHNOLOGY, the manipulation of organisms or their components to make useful products. Biotechnology today usually refers to DNA technology, modern laboratory techniques that involve the manipulation of DNA. RECOMBINANT DNA is formed when scientists combine nucleotide sequences from two different ...
... BIOTECHNOLOGY, the manipulation of organisms or their components to make useful products. Biotechnology today usually refers to DNA technology, modern laboratory techniques that involve the manipulation of DNA. RECOMBINANT DNA is formed when scientists combine nucleotide sequences from two different ...
DNA Technology
... 2. All RFLP’s from sample inserted into a well in a gel 3. An electrical charge is applied to the gel, which causes RFLP’s to migrate down gel according to length (how? chromatography), resulting in a distinct pattern of bands based on genetic code. 4. Gel’s have many wells so that different samples ...
... 2. All RFLP’s from sample inserted into a well in a gel 3. An electrical charge is applied to the gel, which causes RFLP’s to migrate down gel according to length (how? chromatography), resulting in a distinct pattern of bands based on genetic code. 4. Gel’s have many wells so that different samples ...
DNA Notes Part 1
... - Hold all genetic information. - Chromosomes are passed on to an offspring by its parents. Examples: Humans = 46 Shrimp = 254 Chimps = 48 Chicken = 78 Gorilla = 48 Wolf ...
... - Hold all genetic information. - Chromosomes are passed on to an offspring by its parents. Examples: Humans = 46 Shrimp = 254 Chimps = 48 Chicken = 78 Gorilla = 48 Wolf ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.