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File - Science with Mr Thompson
File - Science with Mr Thompson

... is the sequence of these four bases that encodes information. The major function of DNA is to encode the sequence of amino acid residues in proteins, using the genetic code. To read the genetic code, cells make a copy of a stretch of DNA in the nucleic acid RNA. These RNA copies can then used to dir ...
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Okazaki Fragments

... discontinuously against overall direction of replication  This strand is made in MANY short segments It is replicated from the replication fork toward the origin Leading Strand ...
Cell Reproduction
Cell Reproduction

... deoxyribonucleic acid; a cell’s heredity material; made up of two strands, each consisting of a sugar-phosphate backbone and nitrogen bases: adenine, thymine, guanine, and cytosine ...
DNA Workshop
DNA Workshop

... The single molecule of DNA in the bacteria, E. coli contains 4.7 x 106 nucleotide pairs. DNA replication begins at a single, fixed location in this molecule, called the replication origin, it proceeds at about _______ nucleotides per second, and thus is done in approximately _____ minutes. The avera ...
DNA Fingerprinting Lab
DNA Fingerprinting Lab

... One test used in forensic labs is DNA fingerprint. It is also called a DNA profile. Analysts use the DNA profile from potential suspects and compare it against DNA found at a crime scene. There’s DNA profiling for paternity tests. These days you can send a sample of DNA and find out your ancestry to ...
mutations - Pasadena High School
mutations - Pasadena High School

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11-03-11 st bio3 notes

... STRUCTURE OF DNA: -1950's biologists: rush to try to figure out the physical structure of DNA -important names: Watson, Krik, (and Roselyn Franklin though she gets no credit, goes on to have great career -structure indicates replication -sugar/phosphates form the blackbone for the four nucleic acid ...
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cis667-1 - Electrical Engineering and Computer Science

...  Incorrect splicing leads to frame shifts or premature stop codons which make the resulting protein useless  The position of introns is signalled by several specific sequences of nucleotides  Since there is more than one sequence we can have alternative splicing resulting in different proteins be ...
Biotechnology - MRS PITOC
Biotechnology - MRS PITOC

... 1. Understand how bacterial genes can transfer from one cell to another. 1.1. Using diagrams, illustrate how DNA technology transfers bacterial genes from cell to cell. 1.2. Using diagram, illustrate how bacterial plasmids are used as vectors for the transfer of genes from one cell to another. 1.3. ...
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Day 9: DNA Powerpoint

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2015/5/13 9:24 AM

... Is an example of a missense type mutation. 26. A point mutation on a DNA sequence is one in which DNA sequences are inverted. 27. A frame-shift mutation in a DNA sequence changes the translation of every subsequent codon. 28. Large regions of chromosomes can mutate by being deleted or moved to other ...
Scientist Guide DNA Bracelet Workshop
Scientist Guide DNA Bracelet Workshop

... uses a blueprint to construct a house, cells use DNA to construct an organism. DNA is therefore often considered the “blueprint for life.” The DNA instructions are divided into segments called genes. All organisms have genes that determine various biological traits, some of which are immediately vis ...
PowerPoint Presentation - Ch. 10 Molecular Biology of the Gene
PowerPoint Presentation - Ch. 10 Molecular Biology of the Gene

... Greek. • How is this like our study of genetics? • DNA transcribed to RNA translated to a polypeptide. ...
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... Beta-galactosidase NO Beta-galactosidase Gal + X(Blue dye) X-gal White colonies blue colonies (colorless) Allows for easy visual “screening” of bacterial colonies that contain recombinant DNA molecules ...
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Leukaemia Section t(3;11)(q25;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... One case, a 13 yr old boy; occurred 9 yrs after treatment for neuroblastoma with antitopoisomerase II. ...
IB Biology 11 SL (H) - Anoka
IB Biology 11 SL (H) - Anoka

... Outline three outcomes of the sequencing of the complete human genome State that when genes are transferred between species, the amino acid sequence of polypeptides translated from them is unchanged because the genetic code is universal Outline a basic technique used for gene transfer involving plas ...
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First Life Forms Roles of RNA

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From Gene to Protein Protein Synthesis

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CLS 311 Basic Microbiology Lect 9: Bacterial Genatics
CLS 311 Basic Microbiology Lect 9: Bacterial Genatics

... growth of only the desired mutant. We use the Replica plating , which involves the simultaneous transfer of all colonies on one plate to two other plates and the comparison of the growth of individual colonies on both plates. ...
Lecture 27
Lecture 27

... markers for individuality. The number of tandem repeats of STR are unique to an individual. • STRs are amplified from unique sequence outside the tandem repeats. • RNA can be amplified by PCR; first reverse transcribing it to DNA (cDNA) through reverse transcriptase. ...
History of Genetics
History of Genetics

... Despite knowing about inheritance in general, a number of incorrect ideas had to be generated and overcome before modern genetics could arise. 1. All life comes from other life. Living organisms are not spontaneously generated from non-living material. Big exception: origin of life. 2. Species conce ...
University of Groningen Modular assembly of functional DNA
University of Groningen Modular assembly of functional DNA

... The DNA molecule has been used in diverse fields of scientific research such as catalysis, synthesis and nanotechnology due to its special properties. This versatility of the DNA shows the potential of such a molecule to be used in an increasing number of research fields and to create innovative fun ...
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... o How do scientists manipulate DNA to make products useful for humans?p266-272 o How do scientists engineer animals and plants to give them new traits?p274-277 o How can biotechnology improve our lives?p268-270 o Know the process of how to genetically modify an organism. P270-271 o Know the process ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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