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University of Groningen Modular assembly of functional DNA
University of Groningen Modular assembly of functional DNA

... The DNA molecule has been used in diverse fields of scientific research such as catalysis, synthesis and nanotechnology due to its special properties. This versatility of the DNA shows the potential of such a molecule to be used in an increasing number of research fields and to create innovative fun ...
summing-up - Zanichelli online per la scuola
summing-up - Zanichelli online per la scuola

... action of several enzymes: the strands are separated and each acts as a template for the synthesis of a new strand (semiconservative replication). ...
direct genetic testing
direct genetic testing

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... RE's first made it possible to work with small, defined pieces of DNA. Before RE's were discovered, a scientist might be able to tell that a chromosome contained a gene of interest to him. He might be able to purify the protein or use genetic analysis to tell what other genes were close to "his" gen ...
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replication

Complete the blank spaces in the following chart:
Complete the blank spaces in the following chart:

... 5. (Translation/Transcription) converts DNA into mRNA. 6. (mRNA/rRNA) is used to carry the genetic code from DNA to the ribosomes. 7. (DNA/RNA) uses uracil instead of thymine. 8. Transcription takes place in the (nucleus/cytoplasm). Part B: Complete the blank spaces in the following chart then answe ...
AP Biology Study Guide Key Chapter 18
AP Biology Study Guide Key Chapter 18

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Punnett Practice and Notes

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Understanding Genomics
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Restriction Enzymes by Dr. Ty C.M. Hoffman

... The  table  shows  just  a  sample  of  the  many  restriction  endonucleases  that  have  been  discovered  (and   the  bacterial  species  in  which  they  were  discovered).  While  restriction  endonucleases  are  naturally   used  by ...
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Advanced Environmental Biotechnology II

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... There are several chromatin remodeling complexes that use energy provided by hydrolysis of ATP. The SWI/SNF, RSC, and NURF complexes all are very large; there are some common subunits. A remodeling complex does not itself have specificity for any particular target site, but must be recruited by a co ...
Drag and Drop Protein Synthesis Name Period Type in the following
Drag and Drop Protein Synthesis Name Period Type in the following

... 5. Each codon of mRNA (hence each triplet in DNA) codes for one: 6. The specific amino acid carried by a tRNA is determined it's: 7. True or False? When amino acids are brought in by tRNA, they are joined together by hydrolysis reactions to form the growing protein. 8. True or False? Whenever a cell ...
Practicing Protein Synthesis
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SBI4U: Molecular Genetics Unit Review
SBI4U: Molecular Genetics Unit Review

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BL220

... or transmission genetics, molecular biology, and population genetics. During the section on Mendelian genetics, we will cover mitosis and meiosis, traditional genetics problems, modes of inheritance, and chromosomal structure. The section on molecular biology will include information on DNA structur ...
Restriction Enzymes
Restriction Enzymes

... Restriction Endonucleases Recognition sites have symmetry (palindromic) “Able was I, ere, I saw Elba” ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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