• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
proteins
proteins

...  How the information in the DNA results in proteins  Promoter – AUG  Transcription: copy of the gene made on an RNA molecule (messenger RNA, or mRNA ).  This resulting RNA will have exactly the same sequence as one of the strands of the gene but substituting U for T  The strand identical to the ...
Name____________________________ DNA Investigation
Name____________________________ DNA Investigation

... Go to my website. Click on: Goal 3: Inheritance Scroll down, then click on: “Genetics Tour” A) At the top of the web-page, click on “What is DNA?” and watch the slideshow. 1) The DNA strand is made of letters, the letters make words, and the words make sentences. These sentences are called _________ ...
Sequencing a genome - Information Services and Technology
Sequencing a genome - Information Services and Technology

... chromosomes; a physical map may also be a set of overlapping clones ...
Brooker Chapter 10
Brooker Chapter 10

... • Introduces negative supercoils using energy from ATP • It can also relax positive supercoils when they occur ...
As a group, quietly discuss each question and agree
As a group, quietly discuss each question and agree

... • As a group, quietly discuss each question and agree upon one correct answer. The group with the most correct answers will win. ...
Genetic Mutations & Genetic Engineering
Genetic Mutations & Genetic Engineering

... Knowing the sequence of an Organisms DNA allows scientists to study specific genes ...
DNA replication and inheritance File
DNA replication and inheritance File

Study Guide
Study Guide

... 7. Avery and his team isolated Griffith’s transforming principle and performed three tests ...
Sect3DNAReplication - Ecology and Evolutionary Biology
Sect3DNAReplication - Ecology and Evolutionary Biology

... Note that the end result of asexual reproduction in prokaryotes and eukaryotes is the same genetically: • Each daughter cell gets a complete copy of the parental cell genome. • The daughter cells are genetically identical, except for new mutations that occur during the cell cycle (mainly during DNA ...
The iGEM Series
The iGEM Series

... Medical uses of Genetic Engineering 2 S Other uses of this transgenic combination include ...
semester 1 review
semester 1 review

... 44. What is the function of tRNA? 45. If a sequence of nitrogenous bases on a DNA strand is ATCCGA, the corresponding sequence on the mRNA will be ___. 46. Suppose an original strand of DNA reads GTCATC. a. What would the complementary DNA strand read? b. What would the corresponding mRNA strand rea ...
Wednesday, September 5
Wednesday, September 5

... β-globin, a protein found in red blood cells. To obtain sufficient amounts of the protein, you decide to clone the β-globin gene. Would you construct a genomic library or a cDNA library? What material would you use as a source of DNA or RNA? ...
THE CELLULAR AND MOLECULAR BASIS OF INHERITANCE
THE CELLULAR AND MOLECULAR BASIS OF INHERITANCE

... • The backbone of each chain is formed by phosphodiester bonds between the 3' and 5' carbons of adjacent sugars. • The two chains being held together by hydrogen bonds between the nitrogenous bases which point in towards the centre of the helix. • The two DNA strands are held together by hydrogen bo ...
BIO113 Ex 3 sample Q → The questions are NOT comprehensive
BIO113 Ex 3 sample Q → The questions are NOT comprehensive

...  The questions are NOT comprehensive. Review the notes and corresponding textbook sections. These are for practice and are not actual exam questions. 1. DNA is composed of a. A single strand of nucleotides in a particular order b. 2 strands twisted around each other c. Amino acids that form a code ...
Nucleotides
Nucleotides

... form the “backbone” of RNA and DNA • RNAs are far less stable than DNA • Polynucleotides Are Directional Macromolecule – “5′- end” or the “3′- end” – the 5′- end is at the left ...
molecular genetics
molecular genetics

...  Cell makes components (proteins and nucleic acids) of the virus  Cell assembles components into new viruses  New viruses burst out of the cell, resulting in host cell’s death  New viruses can then infect other cells. ...
Genetic Engineering Powerpoint
Genetic Engineering Powerpoint

... the breaking of double-stranded DNA. DNA exhibits a stabilizing interaction between complementary base pairs, providing specificity to the pairing of two strands of DNA. If two complementary strands of DNA are of equal length, then they will terminate in a blunt end, as in the following example: ...
Science 9 Chapter 4 Practice Test
Science 9 Chapter 4 Practice Test

... d. will not be seen until two or three generations have been produced. Substances or factors that cause mutations in DNA are called a. bacteria. c. mutagens. b. diseases. d. mutations. When a cell is preparing to reproduce, the chromatin in the nucleus forms into a. chromosomes. c. genes. b. deoxyri ...
DNA, RNA, and Protein Synthesis Notes Part 1
DNA, RNA, and Protein Synthesis Notes Part 1

... The rungs of the ladder are the complementary paired bases The two DNA strands are anti-parallel (they run in opposite directions) ...
Gral Regents Review Part 2
Gral Regents Review Part 2

... Changes in chromosome structure may also cause disorders. For example, a chromosome may break, leading to a variety of new arrangements that affect its genes. ...
Document
Document

... locations for each strand of the palindromic site. Which enzymes leave compatible ends that will facilitate ligation? a) All of these enzymes leave ends that are compatible with ends generated by the others; b) None of the enzymes produce compatible ends; c) Only BamHI and BglII fragments are compat ...
Restriction Enzyme Digestion
Restriction Enzyme Digestion

... molecule. Exonuclease enzyme digest nucleotides from the ends of the DNA molecule. • 3. What is a restriction endonucleases? Write names of some restriction endouclease. ...
Biotechnology . ppt
Biotechnology . ppt

...  Putting them back into the original organism in different ...
18 Q1 (1 point). Name three amino acids that are typically found at
18 Q1 (1 point). Name three amino acids that are typically found at

... the same two restriction enzymes) into the mcs (see figure), rather than using only a single restriction enzyme. Why does a plasmid constructed with two restriction enzymes (e.g., EcoR1 and BamH1) result in a higher number of correctly modified clones after transformation of the host cell than a pla ...
Genetics Genetics, a discipline of biology, is the science of genes
Genetics Genetics, a discipline of biology, is the science of genes

... DNA is held in the sequence of pieces of DNA called genes. A gene is a sequence of DNA that contains genetic information and can influence the phenotype of an organism. Transmission of genetic information in genes is achieved via complementary base pairing. For example, in transcription, when a cell ...
< 1 ... 507 508 509 510 511 512 513 514 515 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report