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Microbial Genomics - Microbiology and Molecular Genetics
Microbial Genomics - Microbiology and Molecular Genetics

... DNA sequencing of uncultured organisms. An example: Beja et al., 2000. Evidence for a new type of phototrophy in the sea. Science 289: 1902-1906. “Sequence analysis of a 130-kb genomic fragment that encoded the ribosomal RNA (rRNA) operon from an uncultivated member of the marine -Proteobacteria (t ...
Slide 1 - KREISELMANBIOLOGY
Slide 1 - KREISELMANBIOLOGY

... alter the type of proteins produced. Thanks to constant biochemical repair work most mutations are corrected before that have any effect. But in rare cases mutations can accumulate and this can give rise to diseases such as cancer. ...
Genetic engineering and biotechnology
Genetic engineering and biotechnology

... forensic investigations. 4.4.5 Analyse DNA profiles to draw conclusions about paternity or forensic investigations. 4.4.6 Outline three outcomes of the sequencing of the complete human genome. 4.4.7 State that, when genes are transferred between species, the amino acid sequence of polypeptides trans ...
Chapter 12
Chapter 12

... 7. What is a plasmid? 8. Gel electrophoresis separates molecules based on what 2 properties? (Circle which property used in the Lab) 9. Why does DNA move towards the positive end of the electrophoresis box? ...
DNA Structure
DNA Structure

... nitrogen bases across from each other formed hydrogen bonds ...
The Living World
The Living World

...  Capable of forming the entire organism As development proceeds, cells lose their pluripotency  They become committed to one type of tissue  They are then called adult stem cells ...
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... 15. DNA microarray assays 16. SNPs 17. RFLPs ...
dna day becca dillon
dna day becca dillon

... information about DNA as possible. Scientists put out their information that they have found and let other people build on that. DNA would’ve gotten nowhere with out everyone hard work and ...
1 Exam 2 CSS/Hort 430/530 2010 1. The concept of “one gene: one
1 Exam 2 CSS/Hort 430/530 2010 1. The concept of “one gene: one

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The purines In DNA, the pyrimidine bases are

... 1. Purine bases that are consumed in the human diet in the form of DNA or RNA are mostly excreted in the form of uric acid. Xanthine oxidase catalyzes this formation of uric acid from purine bases. 2. The use of tetrahydrofolic acid (TFA) by several of the enzymes in purine and pyrimidine synthesis ...
Reading: Structure of DNA
Reading: Structure of DNA

... The information in DNA is stored as a code made up of four chemical bases: adenine (A), guanine (G), cytosine (C), and thymine (T). Human DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the i ...
Worksheet on DNA and RNA
Worksheet on DNA and RNA

... 8. The process in which DNA builds an exact duplicate of itself is known as _______________________. 9. RNA does not have _____________________ nitrogen base. It is replaced with ____________________. 10. RNA is a ___________________ stranded molecule. 11. The three types of RNA are ________________ ...
Antibiotics - West Chester University of Pennsylvania
Antibiotics - West Chester University of Pennsylvania

... Nuclease – Enzymes that digest or cut DNA and RNA ...
Gene Cloning And DNA vs - Mr. Lesiuk
Gene Cloning And DNA vs - Mr. Lesiuk

... Biotechnology : Using Genetic Engineering to alter genes of bacteria, plants and animals (humans) Gene Therapy is one example of biotechnology. The goal is to alter the phenotype in a human, by altering their genetic makeup. Ex. Child suffering from SCID, now has proper B and T lymphocytes with the ...
Must Knows - Gene Regulation and Biotechnology
Must Knows - Gene Regulation and Biotechnology

... the purpose #5: I of can each compare of the the three types factors of DNA in polymerase mutations and chain their reaction effects(PCR). on the resulting protein. 1. Heat: 2. Primers: 3. Taq polymerase: 11) Which person in the DNA fingerprint shown to the right—Bob, Sue, John, or Lisa—matches the ...
Chapter 19 – Molecular Genetic Analysis and Biotechnology
Chapter 19 – Molecular Genetic Analysis and Biotechnology

... Chapter 19 – Molecular Genetic Analysis and Biotechnology ...
Bozeman DNA Replication Name http://www.youtube.com/watch?v
Bozeman DNA Replication Name http://www.youtube.com/watch?v

... When in the cell cycle does DNA replication occur? What do prokaryotes use as a method to copy their cells? What are the three theories of DNA replication? How did the Meselson-Stahl experiment prove the semi-conservative theory? In the semiconservative theory, where does the DNA split? What are the ...
Photosynthesis - Cathedral High School
Photosynthesis - Cathedral High School

... the HEXA gene on chromosome 15 This causes a stop codon in the wrong location Deficient hexosaminidase (hex A). ...
7529 DNA Sequencing - ACM
7529 DNA Sequencing - ACM

... Finally, Plankton’s attempts to steal the Krabby Patty formula succeeded and it eventually put the Krusty Krab out of business. So, SpongeBob and his co-workers decided to switch to a brand new job. Their new startup is Krusty-Royan, a biological research institute whose main focus is on DNA sequenc ...
Aly Mohamed - Oregon State University
Aly Mohamed - Oregon State University

... GUS cleaves X-Gluc which turns blue after it is cut Mutations in catalitically necessary domains render GUS unable to cleave X-Gluc Blue spots represent a mutation likely due to a decrease in mismatch repair Histochemical staining shows spots of reverted wild type GUS activity arising from frame shi ...
Microarray Cancer Lab - Madison West High School
Microarray Cancer Lab - Madison West High School

... DNA Chip Terminology Probe DNA - short pieces of single stranded DNA attached to glass Target DNA - cDNA from cells grown under different conditions Floating in solution on top of probe DNA example: cDNA from seedlings grown in light vs. dark ...
DNA NOTE RE-CAP (WHAT YOU SHOULD REMEMBER / GOT
DNA NOTE RE-CAP (WHAT YOU SHOULD REMEMBER / GOT

... DNA NOTE RE-CAP (WHAT YOU SHOULD REMEMBER / GOT FROM YESTERDAY!) What does DNA stand for? _________________________________________________ Pieces of a chromosome, or segments of DNA are called ____________________. What do these pieces code for? ____________________________ What is the scientific n ...
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RAD51

... the mutant gene or locus; Rad51: protein. http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae, accessed on Jan 17th, 2011. Alan Wheals. Scanning Electron Micrograph of Saccharomyces cerevisiae. ...
Real-time Quantitative PCR
Real-time Quantitative PCR

... – 1cM, for example • Probably ~ 1 MB or more in humans • Need very many families to get closer than this in human, or very large populations ...
Review Questions - effinghamschools.com
Review Questions - effinghamschools.com

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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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