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The Human Genome Project
The Human Genome Project

... Now we can just look at his genes to see. ...
File
File

... c. cells and structures b. generations d. protein and DN _____ 2. What is the name of the material that determines inherited characteristics? a. deoxyribonucleic acid c. RNA b. ribosome d. amino acid THE PIECES OF THE PUZZLE ...
File
File

... isolated and cut out of a human cell A plasmid is removed from a bacterial cell A piece of the plasmid is removed and the human gene is inserted The recombinant plasmid is inserted back into the bacterial cell The bacterial cell will now produce insulin as a product of its transcription and translat ...
File
File

... isolated and cut out of a human cell A plasmid is removed from a bacterial cell A piece of the plasmid is removed and the human gene is inserted The recombinant plasmid is inserted back into the bacterial cell The bacterial cell will now produce insulin as a product of its transcription and translat ...
TIP Translation - dna
TIP Translation - dna

... ____ 5. The mRNA strand complementary to the DNA sequence TAGTCA is a. ATCAGT. c. GTAGAT. b. AUGAGU. d. AUCAGU. ____ 6. Nitrogenous bases are held to the sides of the DNA ladder by a. helix bonds. c. hydrogen bonds. b. covalent bonds. d. ionic bonds. ____ 7. The first step in making a protein is a. ...
Next lectures: Differential Gene expression
Next lectures: Differential Gene expression

... Site 2 Site 1 ...
Chapter 13 An Introduction to Cloning and Recombinant DNA
Chapter 13 An Introduction to Cloning and Recombinant DNA

... Nuclear Transfer ...
E. coli DNA Gyrase Cleavage Assay Kit
E. coli DNA Gyrase Cleavage Assay Kit

... E. coli DNA gyrase is prepared from the overproducing strains JMtacA and JMtacB (Hallett et al., 1990) and is supplied as an A2B2 complex. The enzyme is supplied at a concentration of 2.0 μM in Dilution Buffer and is suitable for cleavage assays. Cleavage activity is 2 U/μl. 50 % cleavage can be obt ...
Chapter 13 An Introduction to Cloning and Recombinant DNA
Chapter 13 An Introduction to Cloning and Recombinant DNA

... Nuclear Transfer ...
A general video on DNA sequencing is
A general video on DNA sequencing is

... genome, e.g. 3 billion base pairs, what you do is a shotgun approach where you add in a primer of random sequence (at least 16 long), sequence the 400 base sequence, then do it again with another random sequence primer. You then have a whole bunch of 400 long sequences, which means that you will hav ...
Mutation
Mutation

... 2) Frame shifts during replication Frame shifts can be either additions or deletions Both are thought to: - occur during DNA replication - occur at repeated sequences See Fig 14-21(8th) 16-4(7th) Typically detected in protein coding stretches of DNA because they alter the reading frame of triplet co ...
HIV treatments
HIV treatments

... •DNA dependent DNA polymerase then makes opposite DNA strand and then this viral double stranded DNA molecule is incorporated into the cell’s genome •Once inside the body’s cells, the analog has three phosphate groups added to the 5’ OH to become activated •Once bound to the growing DNA strand they ...
Document
Document

... •Mutation refers to a change in a base-pair (e.g. G-C bp to A-T bp is a mutation) •Problems arise when DNA damage is converted to mutation ...
No Slide Title
No Slide Title

... common mechanism for the two types of DNA modification. ...
AIR Genetics Review PPT
AIR Genetics Review PPT

... • Genes can be manipulated through several techniques: – PCR: one copy of DNA is made into many copies – DNA can be cut using restriction enzymes and inserted into a new cell – Gel Electrophoresis: cutting DNA with restriction enzymes and separating the DNA based on size – Cloning: using DNA from an ...
Understanding selectivity in the CRISPR CAS9 system
Understanding selectivity in the CRISPR CAS9 system

... Supervisor: Dr. Stefano Angioletti-Uberti, Materials Description: Gene editing using the CRISPR-CAS9 technology relies on selective recognition of specific nucleotide sequences using a matching RNA of 21 base-pairs. In this system, off-target binding must be reduced to a minimum because its occurren ...
DNA Structure - hrsbstaff.ednet.ns.ca
DNA Structure - hrsbstaff.ednet.ns.ca

... DNA (deoxyribonucleic acid) is found in the nuclei of all cells. It is the DNA that carries the genetic information which will determine all your characteristics (hair color, shape of nose, etc.) Every person’s DNA is different, except ...
How Does DNA Determine the Traits of an Organism
How Does DNA Determine the Traits of an Organism

... AGC CGA GGG TTT AAC AAA GGA CGC CGA GGG AGG AAA ...
Bacterial Genetics
Bacterial Genetics

... The mis-packaged phage infects a new bacterial cell and insert the donor DNA into the recipient cell The donor DNA is integrated and mismatched pairs are repaired ...
L8 Bacterialgenetics 7e
L8 Bacterialgenetics 7e

... Repair of UV damage • Two repair mechanisms – Light repair – Dark repair ...
DNA Polymerase: “ase”
DNA Polymerase: “ase”

...  DNA duplication takes place in the “S” phase of the cell cycle  DNA is found in the nucleus of a eukaryotic cell; linear DNA  DNA is found in the cytoplasm of a prokaryotic cell; single, circular DNA ...
Vocabulary:
Vocabulary:

... nucleotide  bases  are  “attracted”  to  each  other  and  form  a  chemical  bond.     These  4  different  bases  form  chemical  bonds  with  each  other  in  a  very   specific  pattern.  Below  are  the  base  pairing  rules   ...
Study Guide: The Cell
Study Guide: The Cell

... How did Hershey and Chase know that it was the DNA that had infected the bacterial cells in their experiment?  Watson and Crick – What did Watson and Crick create with the model of DNA?  Franklin – ...
Mitochondrial analysis in Forensic Scienses
Mitochondrial analysis in Forensic Scienses

... • This organelle produces energy by oxidising organic acids and fats with oxygen by the process of oxidative phosphorylation and generates oxygen radicals (reactive oxygen species ROS )as a toxic by-product ...
PAG XXIV San Diego 2016 Duckweeds, the smallest flowering
PAG XXIV San Diego 2016 Duckweeds, the smallest flowering

... • The Spirodela genome is one of the smallest among monocots (157 MB) with two ancient WGDs and lack of recent transpositions. • The Spirodela genome has 25% less gene models than Arabidopsis and 50% less than rice. • The Spirodela genome has an expansion and contraction of gene families that correl ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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