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Aim
Aim

... Genetic diversity exists in a population of individuals of the same species. This is usually in terms of differences in nucleotide sequences termed alleles of the gene. Some of these alleles may be transcribed (transform genetic information from DNA into RNA) and then translated (transform genetic i ...
Go to Classzone - Issaquah Connect
Go to Classzone - Issaquah Connect

... A. Go to www.classzone.com, Animated Biology, Chapter 8 (DNA replication) and answer the following questions while completing the simulation. 1. Replication is the process by which DNA is ___________during the cell cycle (interphase). 2. __________________unzip the DNA double helix exposing the nucl ...
Prot Gen Ing Martin Tichy 1.
Prot Gen Ing Martin Tichy 1.

... Why did they choose this Pathogenic organism? 582,970 bp genome Low GC content ~ 30% ...
Steroid Hormones
Steroid Hormones

... Estradiol binds in a deep cleft of a binding site in this mostly helical 240 residue domain of ...
The nucleus as an organized organelle
The nucleus as an organized organelle

... • Individual chromosomes occupy distinct domains within the interphase nucleus without overlapping other chromosomes • The telomeres (ends of chromosomes), and condensed, transcriptionally inactive regions (heterochromatin) are attached to the nuclear envelope (via the lamina) • Ribosomal RNA (rRNA) ...
Additional Glossary and Concepts List for Vertebrate Genetics
Additional Glossary and Concepts List for Vertebrate Genetics

... genome by random integration (cf. gene targeting). Thus, a transgenic construct, unless stated otherwise, means a DNA plasmid designed to be introduced into the genome by transgenesis (pronuclear injection, for example). In my lectures, I will use transgene in this context only. Confusingly, a trans ...
What does DNA look like
What does DNA look like

... (Remember: I like to eat AT Golden Corral.) FIGURE 4 page 146 Making copies of DNA The base pairs allow the cell to copy or replicate the DNA. Bases are complementary (A only pairs with T, C only pairs with G). How copies are made 1. The DNA molecule splits down the middle (unzips down the center of ...
Chapter 47
Chapter 47

... 7. Describe how some of the biotechniques we have studied can be used to diagnose diseases. 8. Describe how scientists can bioengineer viruses to deliver proper genes to tissues that have mutated genes. (Cues: deliver, infect, receptors) 9. Describe how plants are bioengineered? 10. Describe some of ...
Gene Mutations
Gene Mutations

... Mutations – Section 13.3 What is a mutation? When does a mutation occur? ...
(DNA, RNA, or DNA/RNA) Microinjection Service Form
(DNA, RNA, or DNA/RNA) Microinjection Service Form

... cleavage/editing. It is the responsibility of the investigator to confirm whether mutagenesis and/or genome editing has occurred successfully in the resulting mice. • SgRNA-mediated cleavage has been reported to be prone to off-target mutagenesis. These events have been observed in some CRISPR-modif ...
DNA Replication Worksheet
DNA Replication Worksheet

... 1. Draw a wound (spiral) molecule of DNA. Use multiple colors for nitrogen bases, sugars and phosphates. Give your double helix the following sequence: ACCGTATTGATC ...
A new extraction procedure of autonomous DNA
A new extraction procedure of autonomous DNA

... The classical method for extraction of episomes from eucaryotic cells has been described by B.Hirt (1). It is based on precipitation of proteins associated to cellular DNA by sodium chloride, whereas the episomal DNA remains in the supernatant. However, this method does not allow to purify an eventu ...
BACTERIAL GENETICS
BACTERIAL GENETICS

... 1) Homologous recombination: in which two pieces of DNA that has extensive homologous regions pair up and exchange pieces by the process of breakage and reunion 2) Non homologous recombination in which little if any homology is necessary What is Gene Therapy?  Gene therapy is the insertion of genes ...
DNA Discovery and Structure
DNA Discovery and Structure

... ● Hershey and Chase discovered that DNA is the genetic material of bacteriophage (T2) in 1952 ...
NOTES: CH 16 part 1
NOTES: CH 16 part 1

... ● Hershey and Chase discovered that DNA is the genetic material of bacteriophage (T2) in 1952 ...
W09micr430Lec17 - Cal State LA
W09micr430Lec17 - Cal State LA

... DNA glycosylases – catalyzing breakage of the N-glycosyl bond between the base and the sugar, leaving an apyrimidinic or apurinic site. These sites are called AP sites ...
Name____________________________ DNA Investigation
Name____________________________ DNA Investigation

... 1) The DNA strand is made of letters, the letters make words, and the words make sentences. These sentences are called __________. B) At the top of the web-page, click on “What is a Gene?” and watch the slideshow. 2) What is a gene? 3) Approximately how many genes are encoded in the DNA of a human? ...
DNA fingerprinting
DNA fingerprinting

... • The number of the VNTRs can vary significantly from individual to individual • In humans such sequences are often bordered by restriction endonuclease sites. • The fragment sizes resulting from digestion depend on the number of copies between the restriction sites • This gives rise to unique RFLP ...
DNA Technology
DNA Technology

... Applications of Biotechnology Transgenic Animals as Models of Human Diseases ...
Chapter 23 (Part 1)
Chapter 23 (Part 1)

... • Usually present in multiple copies per cell • Plasmids can be cleaved by restriction enzymes, leaving sticky ends • Artificial plasmids can be constructed by linking new DNA fragments to the sticky ends of plasmid ...
ekbdna-structure
ekbdna-structure

... – DNA Ligase seals up any gaps or breaks in the newly created strands ...
Eötvös Loránd Science University Faculty of Sciences Department of
Eötvös Loránd Science University Faculty of Sciences Department of

... 5. DNA introduction into cells (transformation, infection, electroporation). Electrophoresis methods. Hybridization and DNA-chip techniques. Hybridization probes. 6. Polymerase chain reaction and its various applications. 7. Vector-host systems. Pro- and eukaryotic host cells. Plasmids, bacteriophag ...
Restriction Endonucleases • restriction endonucleases
Restriction Endonucleases • restriction endonucleases

... Coregulator - Transcription coregulators are proteins that work with transcription factors to regulate gene expression. Distance measures - used to measure the dissimilarity between the expressions of different genes. DNA Microarray - A DNA microarray is a high-throughput technology used to measure ...
Chromosome structure
Chromosome structure

... replication by 100X. • Remaining errors fixed by Mismatch Repair: – How does system recognize which strand is correct for use as template? – In bacteria, old strands become methylated, repair system recognizes unmethylated new strands. Similar system may work in eukaryotes. ...
Genetic Engineering
Genetic Engineering

... DNA polymerase is the enzyme needed for DNA replication. Scientists are now able to use this enzyme to produce large amounts of DNA from tiny quantities. A solution containing DNA polymerase, the DNA double helix, some of the 4 types of nucleotides, and primers is made. Primers begin the process of ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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