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DNA replication - Understanding Evolution
DNA replication - Understanding Evolution

... Students will understand that 1) molecular mechanisms that preserve the fidelity of the genetic sequence have been favored by natural selection, 2) some entities, such as HIV, lack some of these mechanisms and so have a higher rate of mutation and evolution, and 3) many challenges posed to medical s ...
Notesheet for Sections 13.4, 15.4, and CH16 Basics 13.4
Notesheet for Sections 13.4, 15.4, and CH16 Basics 13.4

... In situ Nucleic Acid HYBRIDIZATION (in situ = in place IN the intact Organism) Fig 15.14 pg 307: ...
ibbiochapter3geneticsppt(1)
ibbiochapter3geneticsppt(1)

... sequence 1-valine-histidine a)_________b)________c)_______d)_________-glutamic acid • sequence 2-valine-histidine e)_________f)_________g)_______h)________glutamic acid • use genetic code to solve the above • this will change the structure of resulting protein-mutation ...
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... GM Animals are regulated under the Hazardous Substances and New Organisms (HSNO) Act by the Environmental Protection Authority (EPA) Long term (until 2030) regulatory approval to develop GM animals in outdoor ...
Life Science I 83.101.102 Dr. Ekaterina (Kate) Vorotnikova Office
Life Science I 83.101.102 Dr. Ekaterina (Kate) Vorotnikova Office

... Gel electrophoresis separates DNA molecules based on size – DNA sample is placed at one end of a porous gel – Current is applied and DNA molecules move from the negative electrode toward the positive electrode – Shorter DNA fragments move through the gel pores more quickly and travel farther throug ...
12711_2011_2534_MOESM1_ESM
12711_2011_2534_MOESM1_ESM

... Blank extractions and several negative PCR controls should be performed alongside extractions and amplifications from ancient material. In fact, the quantity of DNA 2 contamination present in laboratory reagents may be so small that it is detected only sporadically in negative controls. Repeated amp ...
Biotech Overview
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... Primers are required as a starting point for the DNA polymerase, the same enzyme used in DNA replication DNA polymerase then makes copy after copy of the gene. In a couple of hours more copies can be made by PCR than are made using bacteria & cloning vectors ...
Génmanipuláció
Génmanipuláció

... recombinase gene and transient expression of this gene results in recombinatio between the introduced loxP sites to give different products. Type I recombinan ...
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... Each old strand serves as the template for complementary new strand Figure 13.10 Page 223 ...
Mamm_Genome yTrx1-2 + refs
Mamm_Genome yTrx1-2 + refs

... activity (Tagaya et al. 1989). Furthermore, a one-base deletion would initiate a frameshift resulting in a different C-terminus of the protein that has been found to be necessary for protein-protein interaction (Eklund et al. 1991). Fourth, the Trx12 sequence is flanked by a 15 bp direct repeat (wi ...
Review: Unit 3 - Cell Structure, Function and Energy
Review: Unit 3 - Cell Structure, Function and Energy

... A) give the complementary DNA sequence: B) give the mRNA sequence (use the original DNA!): C) tRNA sequence (use the mRNA sequence above!): D) amino acid sequence (use the mRNA codons!): 15) If a mutation occurred in the above DNA strand, in which a “G” was inserted after the 5th nucleotide: A) what ...
Genomics * Reading What we Can*t See
Genomics * Reading What we Can*t See

... Today, we can identify every letter in your genome or of any living species. The progress and rate of discovery of biotechnology is still increasing, while the cost of this work is continually decreasing. ...
unit 5 study guide (ch 12-13)
unit 5 study guide (ch 12-13)

... A) give the complementary DNA sequence: B) give the mRNA sequence (use the original DNA!): C) tRNA sequence (use the mRNA sequence above!): D) amino acid sequence (use the mRNA codons!): 15) If a mutation occurred in the above DNA strand, in which a “G” was inserted after the 5th nucleotide: A) what ...
Nucleotide Sequence Manipulation - ILRI Research Computing
Nucleotide Sequence Manipulation - ILRI Research Computing

... 1.  Small  genomes  ~  5  million  bp  in   bacteria,   in   this   case   gene   binding  is  a  matter  of  identifying   long  ORFs.   2.  E.g.   Haemophelous   inbluenzae   ~85%  of  its  genome  is  in  coding   regions   3.  Calling ...
Teacher Resource 8: Genetic engineering
Teacher Resource 8: Genetic engineering

... Learners work in groups of 2 or 3. Each has a worksheet that they can cut out the statements explaining the process of genetically engineering insulin, including the use of antibiotic marker genes to select plasmids containing the insulin gene. Learners work together to place the statements in the c ...
DNA WebQuest - Pearland ISD
DNA WebQuest - Pearland ISD

... Take the tour of DNA by clicking on “What is DNA?” and answer the questions below: 1. In what organelle (CELL PART) would I find your DNA (YOUR INSTRUCTIONS)? 2. What does DNA stand for? 3. The DNA molecule comes in the form of a ...
042310_recombinant_DNA2
042310_recombinant_DNA2

... copies could be generated) • A recognition sequence for a restriction enzyme (so that we can introduce our DNA of interest) • Reporter genes (to confirm we have successfully introduced the vector into the host cell) • Small size in comparison with host’s chromosomes (for easy manipulation) ...
Chapter 10 DNA Replication and Expression
Chapter 10 DNA Replication and Expression

... – Uracil instead of Thymine ...
Introduction to Next Generation Sequencing
Introduction to Next Generation Sequencing

... • Moores Law: Advances in technology are driving the ability to address questions on a genomic scale • Optimized Array Design Achievable – Requires Control Spike-In Data for Changes in Assay and Oligo Synthesis Approaches – Time consuming and costly • High Throughput Sequencing (Unbiased Functional ...
Nucleic Acids and DNA Replication
Nucleic Acids and DNA Replication

... Creates Okazaki fragments Must wait for a new primer to be placed ...
Ch12 DNA
Ch12 DNA

... Don’t Studied bacteriophages (viruses) “bacteria eaters” copy made of DNA/RNA and protein coat Don’t Bacteriophages inject DNA into bacteria, the viral copy genes act to produce many new bacteriophages and burst of out cell Conclusion: using radioactive markers, genetic material of bacteriophage was ...
Chapter 3 Methods in Molecular Biology and Genetic
Chapter 3 Methods in Molecular Biology and Genetic

... epitope tag. •  epitope tag – A short peptide sequence that encodes a recognition site (“epitope”) for an antibody, typically fused to a protein of interest for detection or purification by the antibody. Human influenza hemagglutinin (HA): YPYDVPDYA The HA tag is derived from the HA-molecule corresp ...
DNA: The Molecule of Heredity
DNA: The Molecule of Heredity

... bases. But there are some major differences-• The sugar in RNA, is Ribose. • RNA is single stranded • the nitrogen bases consist of Uracil (U), Adenine (A), Guanine (G) and Cytosine (C). • Uracil and Adenine = Base Pair • Guanine and Cytosine = Base Pair ...
immunology of infections. hiv
immunology of infections. hiv

... promote CTL priming. • Because of the ability of HIV to undergo recombination, the use of attenuated HIV virus as vaccine has been abandoned for now. • One promising approach is DNA vaccination, in which expression plasmids that drive expression of HIV protein subunits are introduced into host cells ...
Biotechnology
Biotechnology

... DNA into a new bacterium. Recombinant DNA: DNA produced by combining DNA from different organisms ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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