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LECTURE 16 – Using Genomic Variation for Identity DNA Level
LECTURE 16 – Using Genomic Variation for Identity DNA Level

... Ø Bacteria can be infected by viruses known as bacteriophage Ø Inbuilt immunity in the bacteria against bacteriophage is the restriction enzyme – cleaves the DNA of the bacteriophage Ø Bacteria protects its own DNA from the restriction enzymes (by methylation) Ø Restriction enzymes cut the DNA leavi ...
Transcription/Translation
Transcription/Translation

... analyze, alter, study, and recombine DNA sequences • Recombinant DNA is DNA in which nucleotide sequences from two different sources (even different species) are combined in the laboratory to produce a new combination of genes ...
Gene Section IKZF1 (Ikaros family zinc finger 1) in Oncology and Haematology
Gene Section IKZF1 (Ikaros family zinc finger 1) in Oncology and Haematology

... Only 2 cases to date. Hybrid/Mutated gene 5' Ikaros - 3' BCL6 fusion transcript; it is supposed that substitution of the promoter of BCL6 may be responsible for BCL6 deregulation. ...
DNA - The Double Helix
DNA - The Double Helix

... The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded letters --- A, G, T, and C. These bases always bond in a certain way. Adenine will only bond to thymine. Guanine will only bond with cytosine. This is known as the "Base-Pair Rule." The bases can occur ...
DNA
DNA

... • Composed of nucleotides • store and transmit genetic information • replicate • undergo changes (mutate) ...
LATg Training Course - AZ Branch AALAS Homepage
LATg Training Course - AZ Branch AALAS Homepage

... • …focuses specifically on DNA, RNA, and protein • …is a tool used to study genetics ...
Les 1-DNA Structure-review
Les 1-DNA Structure-review

...  Each unique gene has a unique sequence of bases.  This unique sequence of bases will code for the ...
Part I, for Exam 1: 1. Based on Chargaff`s rules, which of the
Part I, for Exam 1: 1. Based on Chargaff`s rules, which of the

... 6. Describe qualitatively how the tm (melting temperature) for a double-stranded DNA depends upon its nucleotide composition. 7. Describe RFLPs and STRs . How is each one used in forensics? Is one better than the other? Why? ...
Biology 3201 - novacentral.ca
Biology 3201 - novacentral.ca

... → restriction endonucleases – family of enzymes made by prokaryotic organisms; these enzymes recognize a short sequence of nucleotides on a strand of DNA and cut the strand at a particular point within a sequence → restriction site – specific location on a strand of DNA where a restriction endonucle ...
Name
Name

... Recombinant DNA – DNA from two different organisms “Carrier” such as a plasmid or a virus Inbreeding – crossing of individuals w/similar characteristics so that those char. will appear in offspring – disadvantage – possibly bringing 2 recessive alleles together ...
Modeling DNA
Modeling DNA

... What  is  a  nucleotide?  What  are  the  parts  of  a  nucleotide?     ...
BE755 Molecular Systems and Synthetic Biology Laboratory
BE755 Molecular Systems and Synthetic Biology Laboratory

... cell lysis, and DNA precipitation. DNA assembly techniques such as Gibson cloning and recombineering. Ethics of genome assembly Lab 4-6: Cloning genetic tools, or circuits, or library that can potentially be publishable. Each student will produce different constructs. Gene expression quantified thro ...
DNA Prot Syn Engineer
DNA Prot Syn Engineer

... Explain why the replication process is a source of few mutations (mismatch repair and excision repair). What are the biochemical differences between RNA and DNA? What are two steps required for the expression of a gene? Briefly explain the process of each Explain why it takes 61 codons to code for 2 ...
Biology 3.3 - Describe the role of DNA in relation to gene
Biology 3.3 - Describe the role of DNA in relation to gene

... • Located in a mobile piece of DNA that contains its own enzymes for moving it around the genome • This piece of DNA is called the Staphylococcal cassette chromosome mec (SCCmec) • Has about 100 ORF on this element – so also contains other genes ...
You should be able to find the information necessary to answer
You should be able to find the information necessary to answer

... concept, or to add more detail to your answer you are encouraged to use other sources (see on-line resources by chapter) 1. Use examples to demonstrate your understanding of the distinction between the following terms; genotype, phenotype, gene, chromosome, and genome. ...
Ionizing radiation And Double
Ionizing radiation And Double

... chromosome is used, no loss of genetic information SSA: Loss of information that lies between sites of homology used in repair. NHEJ: Loss of genetic information due to strand resection. Because both SSA and NHEJ do not utilize homologous chromosomes for repair, it is possible that repair could resu ...
Gene Section CBFb (subunit b of core binding factor)
Gene Section CBFb (subunit b of core binding factor)

... CBF binds to a core motif of the DNA (herein the name); CBFb by itself does not contain any known DNA binding motif or any transcriptional activation domain; CBFa binds to DNA; CBFb increases CBFa's affinity to DNA by 5 to 10 fold; CBF is a transcription factor which regulates the expression of myel ...
Chapter 8 Microbial Genetics
Chapter 8 Microbial Genetics

... • DNA is a long molecule • E.coli chromosome has 4 million base pairs (nucleotides) • DNA is replicated segment by segment ...
on February 28, 2008 Downloaded from www.sciencemag.org
on February 28, 2008 Downloaded from www.sciencemag.org

... protein product. We also demonstrate that structural lesions of this gene are common in DLCL. To isolate normal BCL-6 complementary DNA (cDNA), we screened a cDNA library constructed from the NHL cell line Bjab (11) with a probe (10) derived from the chromosomal region flanking the breakpoints of tw ...
Chromosomes Key - Iowa State University
Chromosomes Key - Iowa State University

... 7. The DNA sequence at the end of chromosomes that consists of -CCC(A/T)- repeats is called what? Why are these important? Telomere – stabilize chromosome; play role in aging ...
What is another name for a polypeptide?
What is another name for a polypeptide?

... Yes, it does. When this happens, we call it a mutation. ...


... ... is the central concept for all of biology. Whenever we say that a mammalian hormone is the ‘same’ hormone as a fish hormone, that a human gene sequence is the ‘same’ as a sequence in a chimp or a mouse, that a HOX gene is the ‘same’ in a mouse, a fruit fly, a frog, and a human -- even when we ar ...
Human Mitochondrial DNA
Human Mitochondrial DNA

... Restriction enzymes are used for transformation (we will do this soon): • Transformation – the uptake and expression of foreign DNA by a cell • Transduction – the use of viruses to transform or genetically engineer cells • Competent/competency – the ability of cells to take up DNA • Selection – the ...
Tutorial_12 (2014)
Tutorial_12 (2014)

... • BLAT on DNA is designed to quickly find sequences of 95% and greater similarity of length 25 bases or more. • BLAT is not BLAST. DNA BLAT works by keeping an index of the entire genome in memory. The index consists of all overlapping 11-mers stepping by 5. • Protein BLAT works in a similar manner ...
Document
Document

... 17. What kind of bonds holds the nitrogen bases together in a DNA molecule? 18. A gene codes for amino acids which are joined together to form _______. 19. Name the 4 nitrogenous bases found in RNA 20. Name the 4 nitrogenous bases found in DNA 21. Which series is arranged in order from largest to sm ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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