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Transformation laboratory
Transformation laboratory

... # of transformants per ug of DNA Our experiment uses: DNA concentration: 0.025 ug ...
E. coli
E. coli

... The concept of a ‘gene’ entered biology The question was “What makes up genes?” Three possibilities: DNA, RNA, or Proteins ...
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Document

... DNA Math: Subtraction The final steps of DNA nicking is the same as adduction. The modified DNA is not always caught and can then be replicated causing more errors and eventually cancer. The first steps are not from unwelcome additions but rather reactive species such as the NO radical that nick of ...
genetic_testD_key
genetic_testD_key

... Determine the nucleotide sequence of the entire human genome Map the location of every gene on each chromosome in humans by the year 2003. HDP will only tell us the location of every gene not the function. ...
DNA (Deoxyribonucleic Acid)
DNA (Deoxyribonucleic Acid)

... to carry the code for proteins out of the nucleus to the ribosomes in the cytoplasm. 2. Describe transcription. RNA polymerase binds to DNA, separates the strands, then uses one strand as a template to assemble MRNA. 3. Why is translation necessary? Translation assures that the right amino acids are ...
Genetics and DNA Replication Notes
Genetics and DNA Replication Notes

... Importance of Protein Synthesis Need all of the following which are proteins or contain proteins…  Enzymes – the catalysts necessary for all reactions in the human body  Hormones – chemical messengers that are required for regulation  Organelle/Cell/Tissue structure – component of organelles such ...
Chapter 12 Study Guide 12.1 Identifying the Substance of Genes
Chapter 12 Study Guide 12.1 Identifying the Substance of Genes

... heat-killed bacteria of one strain could change the inherited characteristics of another strain. He called the process transformation because one type of bacteria (a harmless form) had been changed permanently into another (a disease-carrying form). Because the ability to cause disease was inherited ...
Copying DNA: Southern Blotting
Copying DNA: Southern Blotting

... P C R I S A R A P I D M E T H O D F O R C LO N I N G G E N E S ...
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Introduction to DNA Function and transcription

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Biotechnology Powerpoint
Biotechnology Powerpoint

... A new DNA sequence created when the DNA of one organism is inserted into the DNA of another organism. This “new combination” of DNA is known as recombinant DNA. ...
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DOWNLOAD Middle School Program Flyer

... make a 3-D model of the double helix.($4 materials fee per student) Mutant Organisms (1 hour) Discuss the impact of genetic mutations and observe mutations in fruit flies and/or nematodes using stereo-microscopes. Mendelian Inheritance (1 hour) Use corn as a model to investigate the laws of inherita ...
Lecture #7
Lecture #7

... 3) In a given species, the base composition does not elucidation of the structure of DNA came from model vary with the age of the organism, its nutritional state, building studies. now known as B-DNA (ond of the various forms that DNA can take. or changes in the environment 4) The mole % of A = mole ...
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Presentation

... peptide bonds and a new protein is created. ...
Chemical basis of Inheritance Review KEY - Pelletier Pages
Chemical basis of Inheritance Review KEY - Pelletier Pages

... the 5`-3`direction resulting in a leading and lagging strand made up of okazaki fragments. 11. What is a template? A guide used for complementary base pairing. 12. Lagging strand? Strand of DNA synthesized discontinuously in the 5’-3’ direction resulting in okazaki fragments that must be joined toge ...
Name: Date: Subject: DNA – Structure and Function Objectives
Name: Date: Subject: DNA – Structure and Function Objectives

... uses DNA as an instruction manual. DNA tells the cells how to make proteins that your body uses to function and grow. You can think of like computer code, but only for your cells not your computer. DNA Structure So, you get all of those nucleotides in two long chains that twist around each other. DN ...
DNA Recombinations
DNA Recombinations

... sequence. The result is a set of doublestranded DNA fragments with singlestranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the sticky ends form base pairs with the complementary bases on other DNA molecules. Thus, the sticky ends of DNA fragments can be us ...
DNA, RNA and the Genetic Code Worksheet
DNA, RNA and the Genetic Code Worksheet

... In DNA replication, what determines the sequence of nucleotides in the new strands of nucleotides that form? ...
Name Ch 9 Homework- KEY 1. Cystic fibrosis is a recessive genetic
Name Ch 9 Homework- KEY 1. Cystic fibrosis is a recessive genetic

... and via base pairing, begins to synthesize new daughter strands of DNA. The daughter strand grows in a 5’-3’ direction. Both strands of DNA are synthesizing new daughter strands at the same time. One strand is able to do this continuously (the leading strand) and the other must be synthesized in sho ...
DNA - The Double Helix
DNA - The Double Helix

... alternating sugar and phosphate molecules. The sugar is Deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the Deoxyribose a LIGHT blue (one is labeled with a "D"). The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded lette ...
DNA microarray - Creighton Chemistry Webserver
DNA microarray - Creighton Chemistry Webserver

... Nonhomologous recombination occurs frequently If disrupt essential genes - cell functions/protein altered New evidence that integration events can sometimes activate genes that stimulate cell division (CANCER ENSUES!) Site of integration can have an effect on expression of gene (No way to control th ...
Working with Data The Meselson–Stahl Experiment
Working with Data The Meselson–Stahl Experiment

... was light only, proving the dispersive model false. These results were, however, consistent with a semiconservative model of replication and the structure of DNA that had been proposed by Watson and Crick a few years earlier. The semiconservative model maintains that the original double helix serves ...
DNA: The Genetic Material
DNA: The Genetic Material

... there are 2 strains of Streptococcus: - S strain is virulent - R strain is nonvirulent Griffith infected mice with these strains hoping to understand the difference between the strains ...
• What does DNA stand for? • Where do we find DNA? • How do
• What does DNA stand for? • Where do we find DNA? • How do

... II. DNA Replication A. Cell division produces 2 daughter cells that are genetically identical to each other and genetically identical to the parent cell B. Remember that for this to happen, DNA in the parent cell must be replicated (copied) before cell division -this process occurs during S phase ...
DM1100 - smobio
DM1100 - smobio

Holiday time test notes
Holiday time test notes

... The main concepts of the test this week will be eukaryotic cell structure (chapter 6... the subcellular organelles), the cell cycle, mitosis, DNA replication, and meiosis. We learn about meiosis in the context of animals (humans specifically), but later in the course when we are focusing on differen ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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