G-quadruplex and G-rich sequence stimulate Pif1p
... C The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, ...
... C The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, ...
Mutation, Mutagens, and DNA Repair
... repair has more steps and requires more enzymes than photoreactivation, but it can work on damage created by agents other than UV and on lesions other than pyrimidine dimers. In Escherichia coli bacteria, excision repair requires six proteins: three are involved in finding the damaged region of the ...
... repair has more steps and requires more enzymes than photoreactivation, but it can work on damage created by agents other than UV and on lesions other than pyrimidine dimers. In Escherichia coli bacteria, excision repair requires six proteins: three are involved in finding the damaged region of the ...
Restriction mapping
... The discovery of restriction enzymes, or restriction endonucleases (REs), was pivotal to the development of molecular cloning. REs occur naturally in bacteria, where they specifically recognize short stretches of nucleotides in DNA and catalyze double-strand breaks at or near the recognition site (a ...
... The discovery of restriction enzymes, or restriction endonucleases (REs), was pivotal to the development of molecular cloning. REs occur naturally in bacteria, where they specifically recognize short stretches of nucleotides in DNA and catalyze double-strand breaks at or near the recognition site (a ...
Detection of DNA molecules by SERS spectroscopy with silvered
... variation of the DNA SERS spectra mainly appear due to changes in molecules conformation after their deposition and drying on the silvered PS as well as an increase of randomness under laser irradiation. The intensity and the band position also depend on wavelength and power of the laser and accumul ...
... variation of the DNA SERS spectra mainly appear due to changes in molecules conformation after their deposition and drying on the silvered PS as well as an increase of randomness under laser irradiation. The intensity and the band position also depend on wavelength and power of the laser and accumul ...
DNA Self-assembly Model for Matrix Addition Problem
... are sought to test out the combinatorics of the problem. A sequential or deterministic process of DNA tile selfassembly has three highly parallel instruction steps [40]. The first one is molecular recognition: elementary molecules selectively bind to others. The second is growth: elementary molecule ...
... are sought to test out the combinatorics of the problem. A sequential or deterministic process of DNA tile selfassembly has three highly parallel instruction steps [40]. The first one is molecular recognition: elementary molecules selectively bind to others. The second is growth: elementary molecule ...
Restriction Digest of pAMP and pKAN
... You might have predicted that when loaded into the electrophoresis gel, K- and A- would produce only a single DNA band; there’s no reason why you would think otherwise. However, it is likely that two or three bands will appear in the undigested K- and A- plasmid lanes. This is because plasmids isol ...
... You might have predicted that when loaded into the electrophoresis gel, K- and A- would produce only a single DNA band; there’s no reason why you would think otherwise. However, it is likely that two or three bands will appear in the undigested K- and A- plasmid lanes. This is because plasmids isol ...
III. MATERIAL AND METHODS The present study was undertaken
... the assembly was kept on the bench top so that it made 45-degree angle with the bench top. Assembly was tilted such that one of the bottom corners was raised above the other (off the bench top) and then carefully the solution was poured into the space between the glass plates starting at the lower c ...
... the assembly was kept on the bench top so that it made 45-degree angle with the bench top. Assembly was tilted such that one of the bottom corners was raised above the other (off the bench top) and then carefully the solution was poured into the space between the glass plates starting at the lower c ...
Chapter 2. Structures of Nucleic Acids
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
... mice (Fig. 2.1.A.). Smooth (S) strains produce a capsular polysaccharide on their surface, which allow the Pneumococi to escape destruction by the mouse, and the infection proceeds, i.e. they are virulent. This polysaccaride can be type I, II, or III. Virulent S strains can be killed by heat (i.e., ...
DNA methylation in ciliates: implications in differentiation processes
... The preferential release of MeAde residues in Tetrahymena by these nucleases may be due to a higher number of MeAde in the exposed (linker) regulatory regions of chromatin. The order of increasing thermal stability is MeAde : T < A: T < C : G < MeCyt : G, and easily denaturable sequences may facilit ...
... The preferential release of MeAde residues in Tetrahymena by these nucleases may be due to a higher number of MeAde in the exposed (linker) regulatory regions of chromatin. The order of increasing thermal stability is MeAde : T < A: T < C : G < MeCyt : G, and easily denaturable sequences may facilit ...
Synthesis and isolation of a single-strand
... strands are resistant to degradation (our unpublished data). The Cy5-labeled eluate was mixed with equimolar amounts of the LNA A-modified crude library and the all-DNA FAM-labeled template library generated by lambda exonuclease digestion. KOD DNA polymerase was added and the mixture was left for r ...
... strands are resistant to degradation (our unpublished data). The Cy5-labeled eluate was mixed with equimolar amounts of the LNA A-modified crude library and the all-DNA FAM-labeled template library generated by lambda exonuclease digestion. KOD DNA polymerase was added and the mixture was left for r ...
16 System and a 10X Primer Pair Mix Stored in TE
... this change should not affect amplification results. This change is not necessary for the PowerPlex® Y System because TE-4 buffer is already used to prepare the PowerPlex® Y 10X Primer Pair Mix. We will continue to generate more real time stability data, and if the product shelf life is extended, th ...
... this change should not affect amplification results. This change is not necessary for the PowerPlex® Y System because TE-4 buffer is already used to prepare the PowerPlex® Y 10X Primer Pair Mix. We will continue to generate more real time stability data, and if the product shelf life is extended, th ...
Protein–DNA Hydrophobic Recognition in the Minor Groove
... B-like structures give rise to the differences in the affinities of amino acids to A and B-like nucleotides? Sugar switching and accessibility in the minor groove To quantify the difference between the exposures of the A and B-like nucleotides, we calculated their ASA in the minor groove (see Method ...
... B-like structures give rise to the differences in the affinities of amino acids to A and B-like nucleotides? Sugar switching and accessibility in the minor groove To quantify the difference between the exposures of the A and B-like nucleotides, we calculated their ASA in the minor groove (see Method ...
New beginnings in studies of eukaryotic DNA replication
... type Y' ARS.Iike region but possesses a type X ARS-like region [20]. This ARS.like region contains a perfect match to the AR$ consensus sequence but has not been directly tested for ARS function [20]. Regardless of whether the type X telomeric ARS-fike region functions as an A RS element in plasmids ...
... type Y' ARS.Iike region but possesses a type X ARS-like region [20]. This ARS.like region contains a perfect match to the AR$ consensus sequence but has not been directly tested for ARS function [20]. Regardless of whether the type X telomeric ARS-fike region functions as an A RS element in plasmids ...
Dynamic DNA nanotechnology using strand displacement reactions
... enzyme-based molecular automaton that could perform a computation [56] where the outcome (the release of an antisense drug mimic) was dependent on the ab- ...
... enzyme-based molecular automaton that could perform a computation [56] where the outcome (the release of an antisense drug mimic) was dependent on the ab- ...
13 The Klenow fragment of DNA polymerase I Pol I actually appears
... results in a conformational change in which the thumb closes down around the DNA. • Binding of the correct dNTP forms a productive polymerizing complex (a ternary complex). This favors the correct nucleotide by a factor of 102 to 103. • Once a dNTP is bound, another conformational change occurs in w ...
... results in a conformational change in which the thumb closes down around the DNA. • Binding of the correct dNTP forms a productive polymerizing complex (a ternary complex). This favors the correct nucleotide by a factor of 102 to 103. • Once a dNTP is bound, another conformational change occurs in w ...
Raven/Johnson Biology 8e Chapter 14 - Answers 1.
... factor was DNA. a. If both bacteria are heat-killed, then the transfer of DNA will have no effect since pathogenicity requires the production of proteins encoded by the DNA. Protein synthesis will not occur in a dead cell. b. The nonpathogenic cells will be transformed to pathogenic cells. Loss of p ...
... factor was DNA. a. If both bacteria are heat-killed, then the transfer of DNA will have no effect since pathogenicity requires the production of proteins encoded by the DNA. Protein synthesis will not occur in a dead cell. b. The nonpathogenic cells will be transformed to pathogenic cells. Loss of p ...
A Mathematical Formulation of DNA Computation
... It is well-known that within cells of any living species, there is a substance called Deoxyribonucleic Acid (DNA), which is a double-stranded helix of nucleotides carrying the genetic information of a cell. This information is the code used within cells to form proteins and is the building block upo ...
... It is well-known that within cells of any living species, there is a substance called Deoxyribonucleic Acid (DNA), which is a double-stranded helix of nucleotides carrying the genetic information of a cell. This information is the code used within cells to form proteins and is the building block upo ...
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2
... Our results provide strong evidence that Arabidopsis XRCC2, RAD51B and RAD51D have complex functions in somatic DNA repair and gene regulation, arguing for further studies of these ancient genes that have been maintained in both plants and animals during their long evolutionary history. ...
... Our results provide strong evidence that Arabidopsis XRCC2, RAD51B and RAD51D have complex functions in somatic DNA repair and gene regulation, arguing for further studies of these ancient genes that have been maintained in both plants and animals during their long evolutionary history. ...
Raven/Johnson Biology 8e
... factor was DNA. a. If both bacteria are heat-killed, then the transfer of DNA will have no effect since pathogenicity requires the production of proteins encoded by the DNA. Protein synthesis will not occur in a dead cell. b. The nonpathogenic cells will be transformed to pathogenic cells. Loss of p ...
... factor was DNA. a. If both bacteria are heat-killed, then the transfer of DNA will have no effect since pathogenicity requires the production of proteins encoded by the DNA. Protein synthesis will not occur in a dead cell. b. The nonpathogenic cells will be transformed to pathogenic cells. Loss of p ...
Isolating, Cloning, and Sequencing DNA
... sequences at the rate of 1000 nucleotides per second, around the clock. By related techniques, an isolated gene can be altered (engineered) at will and transferred back into the germ line of an animal or plant, so as to become a functional and heritable part of the organism's genome. These technical ...
... sequences at the rate of 1000 nucleotides per second, around the clock. By related techniques, an isolated gene can be altered (engineered) at will and transferred back into the germ line of an animal or plant, so as to become a functional and heritable part of the organism's genome. These technical ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.