Principles of Genetic Variation
... damage to DNA by altering its chemical structure DNA is a comparatively stable molecule. Nevertheless there are constant threats to its integrity, causing breakage of covalent bonds within DNA or inappropriate bonding of chemicals to DNA. Most of the damage originates spontaneously within cells (nor ...
... damage to DNA by altering its chemical structure DNA is a comparatively stable molecule. Nevertheless there are constant threats to its integrity, causing breakage of covalent bonds within DNA or inappropriate bonding of chemicals to DNA. Most of the damage originates spontaneously within cells (nor ...
Bacterial Screening PCR Kit
... Options for Preparation of Bacterial DNA [Option 1] (Use of 1.5 ml micro test tube with screw cap is recommended.) 1) Wash the pellet containing the separated bacteria (see step 2 in section C above) using sterilized water and TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0) then suspend in a 200 μ l ...
... Options for Preparation of Bacterial DNA [Option 1] (Use of 1.5 ml micro test tube with screw cap is recommended.) 1) Wash the pellet containing the separated bacteria (see step 2 in section C above) using sterilized water and TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0) then suspend in a 200 μ l ...
DNA-Directed Antibody Immobilization for
... immobilization. One such technique, DNA-directed immobilization (DDI), combines the robustness of DNA microarrays with the diagnostic utility of proteins through the use of protein−DNA conjugates to functionalize a DNA surface for subsequent antigen capture.10−19 In DDI, each antibody is encoded by ...
... immobilization. One such technique, DNA-directed immobilization (DDI), combines the robustness of DNA microarrays with the diagnostic utility of proteins through the use of protein−DNA conjugates to functionalize a DNA surface for subsequent antigen capture.10−19 In DDI, each antibody is encoded by ...
Protocols for 16S rDNA Array Analyses of Microbial
... DNA Microarray Analyses SigmaScreen (Sigma) or CMT-GAPS (Corning Inc., Corning, New York) coated slides for microarrays may be used for the DNA array hybridization experiments. One µl (100 pmol/µl) of the complementary primers containing a 15-mer poly T tail at the 5’-end is spotted on the slide. ...
... DNA Microarray Analyses SigmaScreen (Sigma) or CMT-GAPS (Corning Inc., Corning, New York) coated slides for microarrays may be used for the DNA array hybridization experiments. One µl (100 pmol/µl) of the complementary primers containing a 15-mer poly T tail at the 5’-end is spotted on the slide. ...
The presence of two UvrB subunits in the UvrAB complex ensures
... The UvrABC nucleotide excision repair pathway of Escherichia coli is responsible for the removal of a wide variety of structurally unrelated lesions from the DNA. Following damage recognition, the damaged strand is cleaved, ®rst 3¢ then 5¢ to the lesion. The oligonucleotide containing the damage is ...
... The UvrABC nucleotide excision repair pathway of Escherichia coli is responsible for the removal of a wide variety of structurally unrelated lesions from the DNA. Following damage recognition, the damaged strand is cleaved, ®rst 3¢ then 5¢ to the lesion. The oligonucleotide containing the damage is ...
S4 Text.
... Now that we have isolated good quality genomic DNA from our GAL4 enhancer trap lines, we can perform inverse PCR to identify the locations of the pGawB inserts. We will begin by digesting a portion of our genomic DNA with a restriction enzyme that cuts frequently, HpaII. Recombinant DNA technology w ...
... Now that we have isolated good quality genomic DNA from our GAL4 enhancer trap lines, we can perform inverse PCR to identify the locations of the pGawB inserts. We will begin by digesting a portion of our genomic DNA with a restriction enzyme that cuts frequently, HpaII. Recombinant DNA technology w ...
v(d)j recombination: rag proteins, repair factors, and
... can be modeled in synthetic substrates, which show that a RSS pair is used at least 100-fold more frequently than a typical RSS pair (14, 15). The usual arrangement of RSSs at the antigen receptor loci is such that the joined coding segments remain in the chromosome and the junction of the RSSs ...
... can be modeled in synthetic substrates, which show that a RSS pair is used at least 100-fold more frequently than a typical RSS pair (14, 15). The usual arrangement of RSSs at the antigen receptor loci is such that the joined coding segments remain in the chromosome and the junction of the RSSs ...
lecture 1 File
... When bacteriophages (viruses that infect bacteria) infect a bacterial cell, their normal mode of reproduction is to harness the replicational, transcriptional, and translation machinery of the host bacterial cell to make numerous virions, or complete viral particles, including the viral DNA or RNA a ...
... When bacteriophages (viruses that infect bacteria) infect a bacterial cell, their normal mode of reproduction is to harness the replicational, transcriptional, and translation machinery of the host bacterial cell to make numerous virions, or complete viral particles, including the viral DNA or RNA a ...
AxyPrep Blood Genomic DNA Midiprep Kit
... The AxyPrep Blood Genomic DNA Midiprep Kit is designed for the isolation of up to 100 μg of high molecular weight genomic DNA from up to 3 ml of anticoagulated human or animal whole blood or up to 120 μl of anticoagulated avian or amphibian whole blood. The genomic DNA produced by this procedure wil ...
... The AxyPrep Blood Genomic DNA Midiprep Kit is designed for the isolation of up to 100 μg of high molecular weight genomic DNA from up to 3 ml of anticoagulated human or animal whole blood or up to 120 μl of anticoagulated avian or amphibian whole blood. The genomic DNA produced by this procedure wil ...
The effects of teaching style on student learning of DNA
... same topics were covered in both courses, just in different ways. The assessment used as a pretest and posttest was a modification of the National Association of Biology Teachers (NABT) Content Biology test. Both groups were given the pre-test, participated and completed assignments in the classes ...
... same topics were covered in both courses, just in different ways. The assessment used as a pretest and posttest was a modification of the National Association of Biology Teachers (NABT) Content Biology test. Both groups were given the pre-test, participated and completed assignments in the classes ...
A Eukaryotic SWI2/SNF2 Domain, an Exquisite Detector of Double
... Assembly and the corresponding disassembly of multiprotein complexes are known to be essential for the DNA metabolic processes of transcription, recombination, repair, and chromatin remodeling, and the SWI2/SNF2 family of DNA-dependent ATPases is known to be involved in these processes (6). Although ...
... Assembly and the corresponding disassembly of multiprotein complexes are known to be essential for the DNA metabolic processes of transcription, recombination, repair, and chromatin remodeling, and the SWI2/SNF2 family of DNA-dependent ATPases is known to be involved in these processes (6). Although ...
Cutting Edge: DNA Polymerases and Are Dispensable for Ig Gene
... whose contribution is crucial to diversify the third complementarity-determining region of Ig and TCR genes during V(D)J rearrangement; 2) despite a ubiquitous expression, a higher level of transcription in lymphoid tissues, in particular B cells from tonsils, but also, and obviously not in favor of ...
... whose contribution is crucial to diversify the third complementarity-determining region of Ig and TCR genes during V(D)J rearrangement; 2) despite a ubiquitous expression, a higher level of transcription in lymphoid tissues, in particular B cells from tonsils, but also, and obviously not in favor of ...
Polymerase chain reaction (PCR) The polymerase chain reaction
... polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. This DNA polymerase enzymatically assembles a new DNA strand from DNA building-blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required fo ...
... polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. This DNA polymerase enzymatically assembles a new DNA strand from DNA building-blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required fo ...
Exporter la page en pdf
... consist of megabase-scale domains of coordinated origin firing separated by large originless transition regions. Here, we report a quantitative genome-wide analysis of DNA replication kinetics in several human cell types that contradicts this view. DNA combing in HeLa cells sorted into four temporal ...
... consist of megabase-scale domains of coordinated origin firing separated by large originless transition regions. Here, we report a quantitative genome-wide analysis of DNA replication kinetics in several human cell types that contradicts this view. DNA combing in HeLa cells sorted into four temporal ...
as Adobe PDF - Edinburgh Research Explorer
... the activity of the cell cycle regulatory cyclin-dependent kinases in GI (El-Deiry et al., 1993; Gu et al., 1993; Harper et al., 1993; Serrano et al., 1993; Xiong et al., 1993). Additionally, p21 has been found to bind to PCNA, the DNA polymerase 6 auxiliary factor, and p21 itself can prevent synthe ...
... the activity of the cell cycle regulatory cyclin-dependent kinases in GI (El-Deiry et al., 1993; Gu et al., 1993; Harper et al., 1993; Serrano et al., 1993; Xiong et al., 1993). Additionally, p21 has been found to bind to PCNA, the DNA polymerase 6 auxiliary factor, and p21 itself can prevent synthe ...
double-strand break repair pathways in dna structure
... The canonical Watson and Crick B-DNA double helix can reverse its winding direction from a right-hand helix to a left-handed Z-DNA helix in negative supercoiled environments, which can occur, for example, during replication or transcription. Z-DNA structures can form at alternating purine-pyrimidine ...
... The canonical Watson and Crick B-DNA double helix can reverse its winding direction from a right-hand helix to a left-handed Z-DNA helix in negative supercoiled environments, which can occur, for example, during replication or transcription. Z-DNA structures can form at alternating purine-pyrimidine ...
MICROBIAL GENETICS-III UGc - E
... have dCTP, UTP and dTTP (equivalent to TTP). 5_-Mono and -diphosphates are abbreviated as, for example, AMP and dGDP. Nucleoside 5_-triphosphates (NTPs), or deoxynucleoside 5_-triphosphates (dNTPs) are the building blocks of the polymeric nucleic acids. In the course of DNA or RNA synthesis, two pho ...
... have dCTP, UTP and dTTP (equivalent to TTP). 5_-Mono and -diphosphates are abbreviated as, for example, AMP and dGDP. Nucleoside 5_-triphosphates (NTPs), or deoxynucleoside 5_-triphosphates (dNTPs) are the building blocks of the polymeric nucleic acids. In the course of DNA or RNA synthesis, two pho ...
Extranuclear protection of chromosomal DNA from
... enzymes and low molecular antioxidants, ROS can lead to protein, lipid, and DNA oxidation and to cell death (1, 2). Plants contain a large network of genes encoding different pathways involved in ROS scavenging and production, with a key role in managing the overall steady-state level of ROS in cell ...
... enzymes and low molecular antioxidants, ROS can lead to protein, lipid, and DNA oxidation and to cell death (1, 2). Plants contain a large network of genes encoding different pathways involved in ROS scavenging and production, with a key role in managing the overall steady-state level of ROS in cell ...
Chapter 13 - Bellbrook
... determined the central dogma that information flowed from DNA to RNA and from RNA to proteins. However, scientists did not know much about the function of individual genes. Suppose your friend told you the final score of a high school football game but did not tell you how each player contributed to ...
... determined the central dogma that information flowed from DNA to RNA and from RNA to proteins. However, scientists did not know much about the function of individual genes. Suppose your friend told you the final score of a high school football game but did not tell you how each player contributed to ...
Microarray-based DNA methylation profiling: technology and
... dramatically and become one of the most dynamic and rapidly developing branches of molecular biology. The methyl group at the fifth position of the cytosine pyrimidine ring, that is present in about 80% of CpG-dinucleotides in the human genome, can be of major functional significance and is regarded ...
... dramatically and become one of the most dynamic and rapidly developing branches of molecular biology. The methyl group at the fifth position of the cytosine pyrimidine ring, that is present in about 80% of CpG-dinucleotides in the human genome, can be of major functional significance and is regarded ...
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE
... genome will be completed and annotated. The next challenge will be to determine how each of these genes functions alone and with other genes in the genome, to understand the developmental programme of a human. Given that there are many genes that need to be characterized and the fact that a lot of t ...
... genome will be completed and annotated. The next challenge will be to determine how each of these genes functions alone and with other genes in the genome, to understand the developmental programme of a human. Given that there are many genes that need to be characterized and the fact that a lot of t ...
Genome-wide DNA methylation analysis
... Pu: A or G, mC: 5-methylcytosine or 5-hydroxymethylcytosine or N4-methylcytosine , These half-sites can be separated by up to 3 kb, but the optimal separation is 55-103 base pairs Biotechniques. 2010 Oct;49(4):iii-xi ...
... Pu: A or G, mC: 5-methylcytosine or 5-hydroxymethylcytosine or N4-methylcytosine , These half-sites can be separated by up to 3 kb, but the optimal separation is 55-103 base pairs Biotechniques. 2010 Oct;49(4):iii-xi ...
Table of Contents: Introduction
... comparison to neighboring world regions using autosomal STR data. In particular, this analysis will explore evidence for early migrations to Europe from West Asia (including Anatolia and the East Mediterranean) and Siberia (including early relatives of Native Americans). The background section highl ...
... comparison to neighboring world regions using autosomal STR data. In particular, this analysis will explore evidence for early migrations to Europe from West Asia (including Anatolia and the East Mediterranean) and Siberia (including early relatives of Native Americans). The background section highl ...
Site- and strand-specific mismatch repair of
... ds M13ras molecule was cleaved with XhoI and KpnI to produce a 1.8 kb ds fragment (Figure 1, step 3), that was then purified, gently dephosphorylated (to discourage 1.8 kb concatemer ligation; 0.01 U CIP per pmol of 59 termini for 1 h at 37°C) and ligated to the 13.5 kb XhoI–KpnI vector portion of p ...
... ds M13ras molecule was cleaved with XhoI and KpnI to produce a 1.8 kb ds fragment (Figure 1, step 3), that was then purified, gently dephosphorylated (to discourage 1.8 kb concatemer ligation; 0.01 U CIP per pmol of 59 termini for 1 h at 37°C) and ligated to the 13.5 kb XhoI–KpnI vector portion of p ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.