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VWR Taq DNA Polymerase Master Mix
VWR Taq DNA Polymerase Master Mix

Methicillin-resistant Staphylococcus aureus (MRSA) Real
Methicillin-resistant Staphylococcus aureus (MRSA) Real

... Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for difficult-totreat infections in humans. It may also be referred to as multiple-resistant Staphylococcus aureus or oxacillin-resistant Staphylococcus aureus (ORSA). MRSA is especially troublesome in hospital-associated ...
PowerPoint Presentation - Etiology of childhood leukemia
PowerPoint Presentation - Etiology of childhood leukemia

Segmented Arrangement of Borrelia duttonii DNA
Segmented Arrangement of Borrelia duttonii DNA

... inserts are identical. Thus, a total of three different oligonucleotide selected sequences have been cloned; the cross-hybridization data show that these contain no shared sequences substantially larger than the sequence selected by the oligonucleotide probe. Probing uncleaved B. duttonii DNA The VS ...
causes2 - Families Against Cancer & Toxics
causes2 - Families Against Cancer & Toxics

... condition where a fragment of one chromosome is broken off and is then attached to another. ...
DNA THIS ONE
DNA THIS ONE

... T hree parts of a nucleotide: How they pair up, where they bond together and the type of bond that joins them: T ransformation: Griffith: A very: Hershey-Chase: W atson-Crick: DNA replication: List Three differences between DNA & RNA T ranscription: T hree types of RNA: Genetic Code: Codons:: Codons ...
TruSeq™ Sample Preparation Best Practices and Troubleshooting
TruSeq™ Sample Preparation Best Practices and Troubleshooting

... ` DNA quality may also affect the quantity of usable DNA in a sample. For example, if  the DNA is damaged (e.g., heavily nicked or containing extensive apurinic/ apyrimidinic sites), then many of these fragments may fail during library preparation.  ` High molecular weight dsDNA derived from host ge ...
CMSC 838T – Lecture 11 Gene Expression
CMSC 838T – Lecture 11 Gene Expression

... O Diffuse large B-cell vs. follicular lymphomas O Microarray analysis of cancer tissue found significant differences in expression level of 30 of 6817 human genes O 91% correct diagnosis rate substantial improvement O Microarray analysis after treatment predicts survival rates ...
Manual_AccuPrep® Genomic DNA Extraction Kit
Manual_AccuPrep® Genomic DNA Extraction Kit

... 1. Disrupt (or homogenize) the sample (25~50 mg) with a mortar and pestle, place them in a clean 1.5 ml tube (see “Additional required materials”), and add 200 l of Tissue Lysis buffer (TL). Immediately place the weighted, fresh or frozen tissue in liquid nitrogen and grind to a fine powder with mo ...
Insect Karyotype Analysis 1617 - Natomas Unified School District
Insect Karyotype Analysis 1617 - Natomas Unified School District

... chromosomes of the second pair. The extra chromosome of the second pair produces sterile insects that lack coloring in their wings. Since sterility always results, the clear-wing disorder is not passed on to progeny (Figure 3). c. Duplication disorder appears when a portion of a chromosome in t ...
LECTURE #20: Bacterial Transformation and Gel
LECTURE #20: Bacterial Transformation and Gel

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Molecular Biology of the Cell

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Prader-Willi syndrome - type 1 deletion, a

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Chromosome mapping of the sweet potato little leaf
Chromosome mapping of the sweet potato little leaf

... To further understand the genomic diversity and genetic architecture of phytoplasmas, a physical and genetic map of the sweet potato little leaf (SPLL) strain V4 phytoplasma chromosome was determined. PFGE was used to determine the size of the SPLL-V4 genome, which was estimated to be 622 kb. A phys ...
Real Time of PCR - KSU Faculty Member websites
Real Time of PCR - KSU Faculty Member websites

... molecular biology. The SYBR® Green probe was the first to be used in realtime PCR. It binds to double-stranded DNA and emits light when excited. Unfortunately, it binds to any double-stranded DNA which could result in inaccurate data, especially compared with the specificity found in the other two m ...
Gene Sequencing
Gene Sequencing

Toward a Unified Genetic Map of Higher Plants, Transcending the
Toward a Unified Genetic Map of Higher Plants, Transcending the

... is the estimated rate of structural mutation, based on an average rate of 9 pairs of taxa (see Table 1}. Likelihoods are based on a value of L = 100 eM . b-f, Colinearity of monocot and dicot genes. Arabidopsis cDNAs that show DNA sequence conservation (BLASTx > 150; ref. 31} with genes from monocot ...
DNA Repair - WordPress.com
DNA Repair - WordPress.com

... Post Replicative Repair -When DNA polymerase encounters damage in DNA, it cannot proceed. Instead it gives a gap for replication and proceeds up to 800 bp without replicating. Then again it starts replicating after synthesizing a primer by primosome. These gaps are then repaired by using one of the ...
Ch. 1 Plasmids
Ch. 1 Plasmids

... easily sequenced. Moreover, RNA is much less stable than DNA and can degrade quickly. To get around these problems, molecular biologists have developed a technique that allows one to derive double-stranded DNA molecules from single-stranded RNA. The DNA can then be cloned into a vector that makes it ...
Chapter 8: Chromosomes and Chromosomal Anomalies
Chapter 8: Chromosomes and Chromosomal Anomalies

Cloning of PCR products into TOPO TA vectors
Cloning of PCR products into TOPO TA vectors

... why plasmids are considered clinically important. Because plasmids are much smaller in size compared to bacterial and yeast chromosomes, they can be isolated separately from chromosomes. Molecular biologists have thus used plasmids to great advantage by adapting them to serve as vectors to carry "fo ...
Detectie van quarantaine plagen op bedrijven BO-06-005 - q
Detectie van quarantaine plagen op bedrijven BO-06-005 - q

Chapter 11 : BIOTECHNOLOGY-PRINCIPLES
Chapter 11 : BIOTECHNOLOGY-PRINCIPLES

... the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, â-galactosidase (gene gets ‘inactivated due to insertion’ of alien DNA). This results into inactivation of the enzyme, which is ref ...
SafeView - NBS Biologicals
SafeView - NBS Biologicals

... Staining solution may be stored at room temperature and reused. SafeView is non-carcinogenic but may cause skin and eye irritations. Always wear gloves when working with the product. This product is distributed for laboratory research only. CAUTION: Not for diagnostic use. The safety and efficiency ...
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Comparative genomic hybridization



Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.
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