Statistical analysis of DNA microarray data
... Figure 2. Genome-wide Location of the Nine Cell Cycle Transcription Factors(A) 213 of the 800 cell cycle genes whose promoter regions were bound by a myc-tagged version of at least one of the nine cell cycle transcription factors (p < 0.001) are represented as horizontal lines. The weight-averaged b ...
... Figure 2. Genome-wide Location of the Nine Cell Cycle Transcription Factors(A) 213 of the 800 cell cycle genes whose promoter regions were bound by a myc-tagged version of at least one of the nine cell cycle transcription factors (p < 0.001) are represented as horizontal lines. The weight-averaged b ...
Biomolecules
... linkages { These enzymes may digest a DNA strand from the end(s) - exonucleases - or internally endonucleases. Enzymatic hydrolysis of RNA { Ribonucleases are enzymes that cleave RNA August2010 ...
... linkages { These enzymes may digest a DNA strand from the end(s) - exonucleases - or internally endonucleases. Enzymatic hydrolysis of RNA { Ribonucleases are enzymes that cleave RNA August2010 ...
Next-generation DNA sequencing techniques
... even larger sequence output were the need for gels or polymers used as sieving separation media for the fluorescently labelled DNA fragments, the relatively low number of samples which could be analysed in parallel and the difficulty of total automation of the sample preparation methods. These limit ...
... even larger sequence output were the need for gels or polymers used as sieving separation media for the fluorescently labelled DNA fragments, the relatively low number of samples which could be analysed in parallel and the difficulty of total automation of the sample preparation methods. These limit ...
Standard 5 Lesson Plans
... What is DNA? DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitocho ...
... What is DNA? DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitocho ...
FISH MAPPING OF 18S-5.8S
... Triploid viviparous onions, a minor vegetatively reproduced crop which has been cultivated worldwide, probably represent an ancient natural hybrid, whose origin and parental species are still not fully understood (Maass, 1997; Friesen and Klaas, 1998; Puizina et al., 1999). In this study we continue ...
... Triploid viviparous onions, a minor vegetatively reproduced crop which has been cultivated worldwide, probably represent an ancient natural hybrid, whose origin and parental species are still not fully understood (Maass, 1997; Friesen and Klaas, 1998; Puizina et al., 1999). In this study we continue ...
A Recipe for Traits - Learn Genetics (Utah)
... for head shape is always second, and so on. Draw a representation of a chromosome having 8 segments. Have participants come up with a creative name for each gene. Label the segments with the gene names, and specify the trait they encode. Point out that although each dog looks differently (has a diff ...
... for head shape is always second, and so on. Draw a representation of a chromosome having 8 segments. Have participants come up with a creative name for each gene. Label the segments with the gene names, and specify the trait they encode. Point out that although each dog looks differently (has a diff ...
(1) in ppt - NYU Computer Science Department
... abilities (such as the capacity to proliferate and metastasize, ignoring the normal signals that block cellular growth and migration) and liabilities (such as unique dependence on certain cellular pathways, which potentially render them sensitive to certain treatments that spare normal cells). ...
... abilities (such as the capacity to proliferate and metastasize, ignoring the normal signals that block cellular growth and migration) and liabilities (such as unique dependence on certain cellular pathways, which potentially render them sensitive to certain treatments that spare normal cells). ...
The DNA repair helicase UvrD is essential for replication
... • RecG can regress forks quickly and extensively, but not processively • RecG and RecA likely act independently of each other • RecG doesn't like free Mg • …because free cations freeze Holliday junction geometry? • RecG can work on fully duplex 3-stranded structures, but RecA cannot • (since RecA re ...
... • RecG can regress forks quickly and extensively, but not processively • RecG and RecA likely act independently of each other • RecG doesn't like free Mg • …because free cations freeze Holliday junction geometry? • RecG can work on fully duplex 3-stranded structures, but RecA cannot • (since RecA re ...
Quick Look - Strategies for Attaching Oligonucleotides to Solid
... The cross-linkers used to attach thiol-modified oligonucleotides to solid supports are heterobifunctional, meaning that they possess functional groups capable of undergoing a reaction with two chemically distinct function groups, amines and thiols. The linkers serve two purposes: to covalently bind ...
... The cross-linkers used to attach thiol-modified oligonucleotides to solid supports are heterobifunctional, meaning that they possess functional groups capable of undergoing a reaction with two chemically distinct function groups, amines and thiols. The linkers serve two purposes: to covalently bind ...
lecture_23 - supporting lehigh cse
... Annealing produces a double strand from a single strand and its complementary strand Given a test tube of DNA, one can extract a strand that contains some simple pattern of length ‘l’ Using a Polymearse Chain Reaction (PCR), one can detect whether there are DNA strands at all in the test tube All of ...
... Annealing produces a double strand from a single strand and its complementary strand Given a test tube of DNA, one can extract a strand that contains some simple pattern of length ‘l’ Using a Polymearse Chain Reaction (PCR), one can detect whether there are DNA strands at all in the test tube All of ...
File
... XXY = Klinefelter’s syndrome male XXX = Trisomy X female XYY = Jacob’s syndrome male XO = Turner syndrome female ...
... XXY = Klinefelter’s syndrome male XXX = Trisomy X female XYY = Jacob’s syndrome male XO = Turner syndrome female ...
DNA and Life - Science Centre
... building blocks of these living things. Do you know that while a bacterium is made up of only one cell, a human being is made up of trillions of cells! Cells are so tiny that about 5000 of them can fit onto the head of a pin and each cell cannot be seen with the naked eye. However, in this lesson w ...
... building blocks of these living things. Do you know that while a bacterium is made up of only one cell, a human being is made up of trillions of cells! Cells are so tiny that about 5000 of them can fit onto the head of a pin and each cell cannot be seen with the naked eye. However, in this lesson w ...
File
... • Polymerase chain reaction is the outgrowth of knowledge gained from an understanding of how DNA strands naturally replicate within a cell. • For the forensic scientist, PCR offers a distinct advantage in that it can amplify minute quantities of DNA many millions of times. • First, the DNA is heate ...
... • Polymerase chain reaction is the outgrowth of knowledge gained from an understanding of how DNA strands naturally replicate within a cell. • For the forensic scientist, PCR offers a distinct advantage in that it can amplify minute quantities of DNA many millions of times. • First, the DNA is heate ...
A Sex Chromosome Rearrangement in a Human XX
... CerCH-S, which contains the chromosomal segment Xpter-qll. No hybridization signal is observed with hybrids CerCH-H and AS-HRBC2, which lack, respectively, the entire X short arm and the region Xp2Bpter (Figure 3). These data localize the X and Y chromosome common locus U7 to Xp22, the most distal b ...
... CerCH-S, which contains the chromosomal segment Xpter-qll. No hybridization signal is observed with hybrids CerCH-H and AS-HRBC2, which lack, respectively, the entire X short arm and the region Xp2Bpter (Figure 3). These data localize the X and Y chromosome common locus U7 to Xp22, the most distal b ...
Positional dependence of transcriptional inhibition by DNA torsional
... Ct values, with no standard reference, except for the amount of cDNA loaded in each reaction. To conduct the qRT–PCR analysis, we selected a subset of genes from different chromosomes and distances from the telomeres (Supplementary Table 2). The PCR data exposed a clear gradient of transcript ratios ...
... Ct values, with no standard reference, except for the amount of cDNA loaded in each reaction. To conduct the qRT–PCR analysis, we selected a subset of genes from different chromosomes and distances from the telomeres (Supplementary Table 2). The PCR data exposed a clear gradient of transcript ratios ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.