Topic 3.5 powerpoint
... fragments at specific base sequences in each sample. A fluorescent marker binds to a triplet in the DNA fragments, so that results can be seen. Samples are added to a gel electrophoresis chamber. Electric current is passed through, pushing the fragments along. Heavier fragments stay closer to the or ...
... fragments at specific base sequences in each sample. A fluorescent marker binds to a triplet in the DNA fragments, so that results can be seen. Samples are added to a gel electrophoresis chamber. Electric current is passed through, pushing the fragments along. Heavier fragments stay closer to the or ...
chromosomes_nice
... A prokaryotic chromosome consists of a single molecule of DNA in the form of a closed loop. The chromosome is described as circular. A prokaryotic cell has only one chromosome. A eukaryotic chromosome is linear, not circular, in other words it has two ends, like a sausage. Each chromosome contains ...
... A prokaryotic chromosome consists of a single molecule of DNA in the form of a closed loop. The chromosome is described as circular. A prokaryotic cell has only one chromosome. A eukaryotic chromosome is linear, not circular, in other words it has two ends, like a sausage. Each chromosome contains ...
Vectors - Rajshahi University
... fragment to form an artificial chromosome. 3. Transform yeast cells to make a large number of copies. ...
... fragment to form an artificial chromosome. 3. Transform yeast cells to make a large number of copies. ...
Aimhigher Monitoring Template
... them all at some level with the activities on offer. We were also trying to introduce the pupils to a world of higher education outside their sphere of experience and to encourage them that they had the potential to achieve. The new challenge for this year was to engage 6th form students in undergra ...
... them all at some level with the activities on offer. We were also trying to introduce the pupils to a world of higher education outside their sphere of experience and to encourage them that they had the potential to achieve. The new challenge for this year was to engage 6th form students in undergra ...
Notes
... Ordered MUM Selection - Identify the longest set of MUMs such that they occur in order in each of the genomes (using a variation of the well-known algorithm to find the LIS of a sequence of integers) Processing Non-matched Regions - Classify nonmatched regions as either insertions, SNPs or highl ...
... Ordered MUM Selection - Identify the longest set of MUMs such that they occur in order in each of the genomes (using a variation of the well-known algorithm to find the LIS of a sequence of integers) Processing Non-matched Regions - Classify nonmatched regions as either insertions, SNPs or highl ...
C2005/F2401 `07 -- Lecture 19 -- Last Edited
... B. What do you see in a normal squash or karyotype? (Banding, and related topics, may not be covered in this lecture; if we don't get to them they will be discussed next time.) 1. Can see number of chromosomes, size and shape (determined by position of centromere) for each chromosome and can identif ...
... B. What do you see in a normal squash or karyotype? (Banding, and related topics, may not be covered in this lecture; if we don't get to them they will be discussed next time.) 1. Can see number of chromosomes, size and shape (determined by position of centromere) for each chromosome and can identif ...
Recombinant DNA Technology
... Recombinant DNA technology procedures by which DNA from different species can be isolated, cut and spliced together -new "recombinant " molecules are then multiplied in quantity in populations of rapidly dividing cells (e.g. bacteria, yeast). ...
... Recombinant DNA technology procedures by which DNA from different species can be isolated, cut and spliced together -new "recombinant " molecules are then multiplied in quantity in populations of rapidly dividing cells (e.g. bacteria, yeast). ...
typing methods - Micro-Rao
... 2. Restriction Endonuclease Analysis (REA) of Chromosomal DNA A restriction endonuclease enzymatically cuts DNA at a specific nucleotide recognition sequence. The number and sizes of restriction fragments are influenced by the recognition sequence of enzyme and composition of DNA. Bacterial DNA is d ...
... 2. Restriction Endonuclease Analysis (REA) of Chromosomal DNA A restriction endonuclease enzymatically cuts DNA at a specific nucleotide recognition sequence. The number and sizes of restriction fragments are influenced by the recognition sequence of enzyme and composition of DNA. Bacterial DNA is d ...
CloneEZ PCR Cloning Kit
... Fast and precise directional cloning procedures completed within 30 minutes. ...
... Fast and precise directional cloning procedures completed within 30 minutes. ...
AP LAB # 3: MITOSIS AND MEIOSIS
... Consider a sexually reproducing animal with 2 chromosomes, A and B. An animal of this species will possess 2 copies of each chromosome. This is because it receives one chromosome A and one chromosome B from each parent. Thus, it would have chromosomes A1A2 and B1B2. An organism with 2 sets of chromo ...
... Consider a sexually reproducing animal with 2 chromosomes, A and B. An animal of this species will possess 2 copies of each chromosome. This is because it receives one chromosome A and one chromosome B from each parent. Thus, it would have chromosomes A1A2 and B1B2. An organism with 2 sets of chromo ...
Full-Text PDF
... is an essential step in population analysis, especially for next generation sequencing applications. Many nanoparticles as well as naturally occurring clay minerals contain charged surfaces or edges that capture negatively charged DNA molecules after cell lysis within DNA extraction. Depending on th ...
... is an essential step in population analysis, especially for next generation sequencing applications. Many nanoparticles as well as naturally occurring clay minerals contain charged surfaces or edges that capture negatively charged DNA molecules after cell lysis within DNA extraction. Depending on th ...
Protocol Booklet
... signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction is important for understanding cellular process. Chromatin immun ...
... signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction is important for understanding cellular process. Chromatin immun ...
03-Biological 42-3-Rosa
... native to this water system and are absolutely free from any kind of introduction or translocation. Metaphase chromosomes were obtained through the air drying method (Bertollo et al., 1978) from kidney cells. The chromosomal classification was based on arm ratio, according to Levan et al. (1964). In ...
... native to this water system and are absolutely free from any kind of introduction or translocation. Metaphase chromosomes were obtained through the air drying method (Bertollo et al., 1978) from kidney cells. The chromosomal classification was based on arm ratio, according to Levan et al. (1964). In ...
Gel electrophoresis of restriction digest
... for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample wells and the gel is run at a volatage and for a time period that will achieve optimal separation; and (3) the gel is stained or, if ethidium bromide has been incorporated into the gel and electrophoresis b ...
... for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample wells and the gel is run at a volatage and for a time period that will achieve optimal separation; and (3) the gel is stained or, if ethidium bromide has been incorporated into the gel and electrophoresis b ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.