How Can Transposons Accelerate Your Genomics
... resistance characteristics and endogenous restriction systems. Bacterial strain of interest: – Ensure antibiotic resistance markers and promoters in your transposon are functional in your organism of interest. – Identify appropriate antibiotic concentration with a Minimum Inhibitory ...
... resistance characteristics and endogenous restriction systems. Bacterial strain of interest: – Ensure antibiotic resistance markers and promoters in your transposon are functional in your organism of interest. – Identify appropriate antibiotic concentration with a Minimum Inhibitory ...
The Expression in Staphylococcus aureus of Cloned DNA Encoding
... resistant/sensitive strain pair constructed by transduction. Furthermore, MF5 showed strong homology to a mec-associated fragment of identical length and endonuclease map which had been cloned independently by Beck et al. (1986). MF5 was later shown to be present at one end of a larger section of ch ...
... resistant/sensitive strain pair constructed by transduction. Furthermore, MF5 showed strong homology to a mec-associated fragment of identical length and endonuclease map which had been cloned independently by Beck et al. (1986). MF5 was later shown to be present at one end of a larger section of ch ...
DNA Replication in Bacteria
... In prokaryotes replication proceeds at about 1000 nucleotides per second, and thus is done in no more than 40 minutes. ...
... In prokaryotes replication proceeds at about 1000 nucleotides per second, and thus is done in no more than 40 minutes. ...
Deception Through Terminology - Part 1 of 7
... Definition: "Microevolution" means variety within the same species, meaning variety within the same DNA structure. Remember we defined a DNA structure to be a unique set of genes, algorithms, etc. "Microevolution" means that within a specific species the gene structures must be the same, but the ind ...
... Definition: "Microevolution" means variety within the same species, meaning variety within the same DNA structure. Remember we defined a DNA structure to be a unique set of genes, algorithms, etc. "Microevolution" means that within a specific species the gene structures must be the same, but the ind ...
learning outcomes - McGraw Hill Higher Education
... clone libraries for sequencing; internal transcribed spacer regions (ITS) between rRNA genes also has been used; PCR biases exist that confound results to some extent 3. Hierarchical oligonucleotide primer extension (HOPE) allows the detection of multiple SSU rRNA genes in a single sample; different ...
... clone libraries for sequencing; internal transcribed spacer regions (ITS) between rRNA genes also has been used; PCR biases exist that confound results to some extent 3. Hierarchical oligonucleotide primer extension (HOPE) allows the detection of multiple SSU rRNA genes in a single sample; different ...
DNA Hybridization: A Decade of Molecular Discourse in Hominoid
... gained an appreciation of the research in hominoid phylogeny, prior to the introduction of DNA hybridization. Second, the reader should now have a clear understanding of DNA hybridization. Not only are these points essential for an lillderstanding of any discussion on the contribution ofDNA hybridiz ...
... gained an appreciation of the research in hominoid phylogeny, prior to the introduction of DNA hybridization. Second, the reader should now have a clear understanding of DNA hybridization. Not only are these points essential for an lillderstanding of any discussion on the contribution ofDNA hybridiz ...
Southern molecular hybridization experiments with parallel
... complementary probes. Fig. 4 demonstrates the autoradiogram after hybridization of duplicate nitrocellulose filters. The quality of signals obtained with both probes is very close and permits one to use parallel probes for isolation of clones fi'om genomic libraries. We have isolated the correspondi ...
... complementary probes. Fig. 4 demonstrates the autoradiogram after hybridization of duplicate nitrocellulose filters. The quality of signals obtained with both probes is very close and permits one to use parallel probes for isolation of clones fi'om genomic libraries. We have isolated the correspondi ...
Corning® Epoxide Coated Slides Instruction Manual
... fabrication of oligonucleotide arrays for applications including transcriptional profiling and SNP detection, and when substrate stability and consistency are absolute requirements. Epoxide Coated Slides have a uniform coating of a proprietary epoxide chemistry that enables covalent attachment of un ...
... fabrication of oligonucleotide arrays for applications including transcriptional profiling and SNP detection, and when substrate stability and consistency are absolute requirements. Epoxide Coated Slides have a uniform coating of a proprietary epoxide chemistry that enables covalent attachment of un ...
Rapid and simple method for DNA extraction from plant and algal
... and liquid nitrogen-frozen tobacco leaf discs, demonstrating that DNA obtained from frozen plant samples using this method is suitable for PCR amplification. Homogenization of plant tissue is considered to be critical for successful PCR amplification after rapid DNA extraction. To test this assumpti ...
... and liquid nitrogen-frozen tobacco leaf discs, demonstrating that DNA obtained from frozen plant samples using this method is suitable for PCR amplification. Homogenization of plant tissue is considered to be critical for successful PCR amplification after rapid DNA extraction. To test this assumpti ...
How Does Replication-Associated Mutational Pressure Influence
... ORFs were distributed evenly on the chromosome inorg and http://smorfland. microb.uni.wroc.pl). dependently of W or C strands or leading/lagging DNA The analysis of DNA walks on W and C strands strands. Figure 1b presents the same DNA walks for T. done for the third positions in coding sequences and ...
... ORFs were distributed evenly on the chromosome inorg and http://smorfland. microb.uni.wroc.pl). dependently of W or C strands or leading/lagging DNA The analysis of DNA walks on W and C strands strands. Figure 1b presents the same DNA walks for T. done for the third positions in coding sequences and ...
Chapter 7: The New Genetics—Techniques for DNA Analysis
... The DNA of an individual—I will use myself as the example—is then purified and the bonds connecting the two strands of the DNA molecule are cut, making the DNA single stranded. I happen to be a heterozygote at the locus at which the probe will bind. The difference in the alleles is subtle, but it ap ...
... The DNA of an individual—I will use myself as the example—is then purified and the bonds connecting the two strands of the DNA molecule are cut, making the DNA single stranded. I happen to be a heterozygote at the locus at which the probe will bind. The difference in the alleles is subtle, but it ap ...
Escherichia coli
... Positive control For copy number determination and as a positive control for the PCR set up, the kit contains a positive control template. This can be used to generate a standard curve of E. coli-spp copy number / CT value. Alternatively the positive control can be used at a single dilution where fu ...
... Positive control For copy number determination and as a positive control for the PCR set up, the kit contains a positive control template. This can be used to generate a standard curve of E. coli-spp copy number / CT value. Alternatively the positive control can be used at a single dilution where fu ...
Karyotyping
... Females have only one type of sex chromosome, which is called an X chromosome. Males have two types of sex chromosomes, an X chromosome and a much smaller Y chromosome. Figure 1 on the next page shows each of the 22 types of autosomes and the 2 types of sex chromosomes. A karyotype is a diagram that ...
... Females have only one type of sex chromosome, which is called an X chromosome. Males have two types of sex chromosomes, an X chromosome and a much smaller Y chromosome. Figure 1 on the next page shows each of the 22 types of autosomes and the 2 types of sex chromosomes. A karyotype is a diagram that ...
A model for repair of radiation-induced DNA double
... The bacterium Deinococcus (formerIy Micrococcus) radiodurans and other members of the eubacterial family Deinococaceae are extremely resistant to ionizing radiation and many other agents that damage DNA. Stationary phase D. radiodurans exposed to 1 .O-1.5 Mrad y-irradiation sustains >120 DNA double- ...
... The bacterium Deinococcus (formerIy Micrococcus) radiodurans and other members of the eubacterial family Deinococaceae are extremely resistant to ionizing radiation and many other agents that damage DNA. Stationary phase D. radiodurans exposed to 1 .O-1.5 Mrad y-irradiation sustains >120 DNA double- ...
Teacher`s guide - National Centre for Biotechnology Education
... concentrate. The loss of a small amount of EDTA should not affect the electrophoresis — simply filter the crystals out of the solution. If the buffer concentrate crystallises significantly, however, you should obtain a new supply. The TBE buffer can be re-used three or four times if desired. Eventua ...
... concentrate. The loss of a small amount of EDTA should not affect the electrophoresis — simply filter the crystals out of the solution. If the buffer concentrate crystallises significantly, however, you should obtain a new supply. The TBE buffer can be re-used three or four times if desired. Eventua ...
Cot-1 banding of human chromosomes using fluorescence
... labeling. Clear green signals of probe DNA on the red banded chromosomes can be obtained and the banding resolution is as clear as Q-banding. By this method, we mapped a 4-kb genomic DNA fragment from myelin protein zero (MPZ) gene on chromosome 1q22 to q23 (Fig. 4) that is consistent with our previ ...
... labeling. Clear green signals of probe DNA on the red banded chromosomes can be obtained and the banding resolution is as clear as Q-banding. By this method, we mapped a 4-kb genomic DNA fragment from myelin protein zero (MPZ) gene on chromosome 1q22 to q23 (Fig. 4) that is consistent with our previ ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.