Package `rDNA`
... argument provides a constant by which edge values are multiplied. This is useful because normalized edge weights in the time window algorithm may become quite small. ...
... argument provides a constant by which edge values are multiplied. This is useful because normalized edge weights in the time window algorithm may become quite small. ...
transformation
... In step 9, the sticky ends of the Plasmid DNA joined with the sticky ends of the Gene Insert to form the genetically engineered stretch of DNA that was intended. However, since the Ligase will cause any matching sticky ends to join, it is just as likely that the two sticky ends of the original Plasm ...
... In step 9, the sticky ends of the Plasmid DNA joined with the sticky ends of the Gene Insert to form the genetically engineered stretch of DNA that was intended. However, since the Ligase will cause any matching sticky ends to join, it is just as likely that the two sticky ends of the original Plasm ...
Measuring Double-Stranded DNA Concentration Using the Quantus
... a wide variety of biological applications. These include standard molecular biology techniques such as constructing cDNA libraries, purifying DNA fragments or subcloning, quantitating DNA amplification products and detecting DNA molecules in drug preparations. ...
... a wide variety of biological applications. These include standard molecular biology techniques such as constructing cDNA libraries, purifying DNA fragments or subcloning, quantitating DNA amplification products and detecting DNA molecules in drug preparations. ...
Paper Clip PCR.pub
... Reaction). PCR is a method used in the lab to make many copies of a specific piece of DNA that we want to study. This process uses repeated cycles of heating and cooling to separate DNA strands and make new copies of the desired DNA segment. Project for students the Virtual PCR lab found on the Univ ...
... Reaction). PCR is a method used in the lab to make many copies of a specific piece of DNA that we want to study. This process uses repeated cycles of heating and cooling to separate DNA strands and make new copies of the desired DNA segment. Project for students the Virtual PCR lab found on the Univ ...
Visualization of oligonucleotide probes and point mutations in
... using phosphoramidite chemistries. The sequences of probe-primer ODNs, P1 ‘‘anchor’’ probes, AD-ODNs, circles, and decorator probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated fr ...
... using phosphoramidite chemistries. The sequences of probe-primer ODNs, P1 ‘‘anchor’’ probes, AD-ODNs, circles, and decorator probes for RCA reactions are listed in Tables 1 and 2. The probe-primer ODNs and the AD-P2-ODNs were designed to have two 3⬘ ends. The synthesis of these ODNs was initiated fr ...
Colorectal cancer : Three pathways
... Colorectal cancer: the pathways 2. MSI pathway(Microsatellite instability) Micro satellite : SSR(Simple Sequence Repeat) STR(Short Tandem Repeat) Microsatellites are nucleotide repeat sequences scattered throughout the genome and MSI refers to a discrepancy, and thus instability, in the number of n ...
... Colorectal cancer: the pathways 2. MSI pathway(Microsatellite instability) Micro satellite : SSR(Simple Sequence Repeat) STR(Short Tandem Repeat) Microsatellites are nucleotide repeat sequences scattered throughout the genome and MSI refers to a discrepancy, and thus instability, in the number of n ...
Chapter 13
... • The polymerase-DNA complex is stabilized by a sliding DNA clamp, a protein that keeps the enzyme and DNA in close contact. ...
... • The polymerase-DNA complex is stabilized by a sliding DNA clamp, a protein that keeps the enzyme and DNA in close contact. ...
DNA Denaturing through UV-C Photon Dissipation: A
... The yeast DNA was dissolved in a Dulbecco PBS buffer and the salmon sperm DNA dissolved in purified water (Mili-q). The resulting concentrations of double helix DNA were determined from their absorption spectrum to be 2.2, 0.7, 0.0015 and 0.00023 µM for the 25 bp synthetic, 48 bp synthetic, yeast an ...
... The yeast DNA was dissolved in a Dulbecco PBS buffer and the salmon sperm DNA dissolved in purified water (Mili-q). The resulting concentrations of double helix DNA were determined from their absorption spectrum to be 2.2, 0.7, 0.0015 and 0.00023 µM for the 25 bp synthetic, 48 bp synthetic, yeast an ...
Homogenisation in the ribosomal RNA genes of an Epichloe
... forbearance, beers, inspiration, etc., etc. - a nigh-on impossible task! So to everyone who I omit to mention personally - cheers , I'll buy you a beer sometime! First I want to thank my parents, Janine and Garth, for their unconditional love and support throughout. It was them who instilled in me t ...
... forbearance, beers, inspiration, etc., etc. - a nigh-on impossible task! So to everyone who I omit to mention personally - cheers , I'll buy you a beer sometime! First I want to thank my parents, Janine and Garth, for their unconditional love and support throughout. It was them who instilled in me t ...
Investigating a Eukaryotic Genome
... between compatible restriction endonuclease-generated ends on vector and source DNA fragments. This experiment is designed to illustrate how recombinant plasmids are made and analyzed. The recombinant plasmids made in this week's experiment are a combination of yeast genomic DNA and bacterial plasmi ...
... between compatible restriction endonuclease-generated ends on vector and source DNA fragments. This experiment is designed to illustrate how recombinant plasmids are made and analyzed. The recombinant plasmids made in this week's experiment are a combination of yeast genomic DNA and bacterial plasmi ...
Digital PCR Analysis of Maternal Plasma for
... genetic disorders (10 ); RHD (Rh blood group, D antigen) gene typing in Rh D–negative mothers (11 ); diagnosis of monogenic disorders, such as achondroplasia (12 ) or torsion dystonia (13 ); and the exclusion of affected status in autosomal recessive disorders, such as  thalassemia and cystic fibro ...
... genetic disorders (10 ); RHD (Rh blood group, D antigen) gene typing in Rh D–negative mothers (11 ); diagnosis of monogenic disorders, such as achondroplasia (12 ) or torsion dystonia (13 ); and the exclusion of affected status in autosomal recessive disorders, such as  thalassemia and cystic fibro ...
Adobe PDF - Boston University Physics
... GA, AG, TC, CT sed, and GC, CG shd for the complete yeast genome (total length 6 325 440 base pairs (bp); total length of the coding DNA 3 549 593 bp). We use this classification because A is complementary to T , and C is complementary to G; also, we average over two possible directions of reading o ...
... GA, AG, TC, CT sed, and GC, CG shd for the complete yeast genome (total length 6 325 440 base pairs (bp); total length of the coding DNA 3 549 593 bp). We use this classification because A is complementary to T , and C is complementary to G; also, we average over two possible directions of reading o ...
Photosynthesis
... - Allelic patterns of grandparents will likely to be disrupted in parental gametes with all allelic combinations possible If the three genetic loci occur in close sequence on the chromosome - Crossing over very UNlikely to occur between loci - Allelic patterns of grandparents will likely to be pre ...
... - Allelic patterns of grandparents will likely to be disrupted in parental gametes with all allelic combinations possible If the three genetic loci occur in close sequence on the chromosome - Crossing over very UNlikely to occur between loci - Allelic patterns of grandparents will likely to be pre ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.