Document
... • The DNA replication machine may be stationary during the replication process • Recent studies support a model in which DNA polymerase molecules “reel in” parental DNA and “extrude” newly made daughter DNA molecules ...
... • The DNA replication machine may be stationary during the replication process • Recent studies support a model in which DNA polymerase molecules “reel in” parental DNA and “extrude” newly made daughter DNA molecules ...
Recent progress on the Ada response for inducible repair of DNA
... adaptive response in other microorganisms. Furthermore, certain algae and fungi growing in saline environments generate MeCl as a product of chloride detoxification (Sedgwick and Vaughan, 1991). MeCl is probably the most abundant methylating agent in our environment (Crutzen and Andreae, 1990). Chem ...
... adaptive response in other microorganisms. Furthermore, certain algae and fungi growing in saline environments generate MeCl as a product of chloride detoxification (Sedgwick and Vaughan, 1991). MeCl is probably the most abundant methylating agent in our environment (Crutzen and Andreae, 1990). Chem ...
REAL-TIME PCR
... fluorophore (Higuchi et al., 1992), SYBR green is the preferred dye because its binding affinity to doublestranded DNA (dsDNA) is more than 100 times higher than that of ethidium bromide and it does not interfere with the amplification process since it binds to the minor groove of the dsDNA (Witter ...
... fluorophore (Higuchi et al., 1992), SYBR green is the preferred dye because its binding affinity to doublestranded DNA (dsDNA) is more than 100 times higher than that of ethidium bromide and it does not interfere with the amplification process since it binds to the minor groove of the dsDNA (Witter ...
What are chromosomes?
... the intervening segment is rejoined in an inverted or opposite manner. Since there is no loss nor gain of chromosomal material, inversion carriers are normal Paracentric: does not include the centromere pericentric:inverted segment contains the centromere In meiosis, the normal chromosome and the in ...
... the intervening segment is rejoined in an inverted or opposite manner. Since there is no loss nor gain of chromosomal material, inversion carriers are normal Paracentric: does not include the centromere pericentric:inverted segment contains the centromere In meiosis, the normal chromosome and the in ...
Regions of XY homology in the pig X pseudoautosomal region
... estimated to be about 50 Mb in length by flow cytometry [9]. Early studies looking at the banding pattern of the Y noted that the long arm (Yq) contains a large C band, indicating that this arm contains a substantial proportion of constitutive heterochromatin [3,10]. Subsequent physical mapping of b ...
... estimated to be about 50 Mb in length by flow cytometry [9]. Early studies looking at the banding pattern of the Y noted that the long arm (Yq) contains a large C band, indicating that this arm contains a substantial proportion of constitutive heterochromatin [3,10]. Subsequent physical mapping of b ...
McCance, J. An attempt at isolating and characterizing segmented
... these enzymes generates a number of restriction enzymes such as Rsal. Digesting with DNA sequence of the bacteria being fragments, which vary in length depending on the used. The fluorescent end labeled investigated and the restriction enzyme being electrophoresis and laser-induced fragments may now ...
... these enzymes generates a number of restriction enzymes such as Rsal. Digesting with DNA sequence of the bacteria being fragments, which vary in length depending on the used. The fluorescent end labeled investigated and the restriction enzyme being electrophoresis and laser-induced fragments may now ...
BioTeke Corporation
... inactivate cellualar nuclease after remove paraffin, then DNA selectively absorbed on silica membrane in high salt solution, after that take a serial of elution- centrifugation step to remove cellular metabolite and proteins etc. Finally use low salt elution to elute purified genome DNA from silica ...
... inactivate cellualar nuclease after remove paraffin, then DNA selectively absorbed on silica membrane in high salt solution, after that take a serial of elution- centrifugation step to remove cellular metabolite and proteins etc. Finally use low salt elution to elute purified genome DNA from silica ...
Biochemistry - Stryer - Science and Technology
... Several hundred restriction enzymes have been purified and characterized. Their names consist of a three-letter abbreviation for the host organism (e.g., Eco for Escherichia coli, Hin for Haemophilus influenzae, Hae for Haemophilus aegyptius) followed by a strain designation (if needed) and a roman ...
... Several hundred restriction enzymes have been purified and characterized. Their names consist of a three-letter abbreviation for the host organism (e.g., Eco for Escherichia coli, Hin for Haemophilus influenzae, Hae for Haemophilus aegyptius) followed by a strain designation (if needed) and a roman ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... clinical and behavioral phenotype. This occurs because of an imbalance of normal dosage of genes that are present in that segment of chromosome. Many clinical characteristics of the well-known microdeletion syndromes are very specific and have been well defined. It is not always possible to detect t ...
... clinical and behavioral phenotype. This occurs because of an imbalance of normal dosage of genes that are present in that segment of chromosome. Many clinical characteristics of the well-known microdeletion syndromes are very specific and have been well defined. It is not always possible to detect t ...
Case Report Section
... metaphases. Fluorescence in situ hybridization study showed rearrangement of the MLL gene in interphase and metaphase cells revealing that the break-apart 5'MLL segment is translocated to the derivative X chromosome. The patient achieved a complete hematological remission with chemotherapy one month ...
... metaphases. Fluorescence in situ hybridization study showed rearrangement of the MLL gene in interphase and metaphase cells revealing that the break-apart 5'MLL segment is translocated to the derivative X chromosome. The patient achieved a complete hematological remission with chemotherapy one month ...
RECIPROCAL CHROMOSOME TRANSLOCATIONS IN 437
... standards (WHO 1999). Cytogenetic preparations were obtained from phytohaemagglutinin (PHA) stimulated peripheral blood lymphocytes. For cytogenetic analysis, peripheral blood samples were prepared according to the standard laboratory protocol (Rooney, Czepulkowski 1992). The cultures were incubated ...
... standards (WHO 1999). Cytogenetic preparations were obtained from phytohaemagglutinin (PHA) stimulated peripheral blood lymphocytes. For cytogenetic analysis, peripheral blood samples were prepared according to the standard laboratory protocol (Rooney, Czepulkowski 1992). The cultures were incubated ...
DNA-dependent DNA polymerase (DDDP)
... bidirectional from one origin, and the two replication forks must meet at one point called ter at 32. • All the primers will be removed, and all the fragments will be connected by DNA-pol I and ligase. ...
... bidirectional from one origin, and the two replication forks must meet at one point called ter at 32. • All the primers will be removed, and all the fragments will be connected by DNA-pol I and ligase. ...
hybrid DNA molecules
... of the cloned his3 gene that delete DNA sequences near or in the structural gene have been isolated and physically defined (unpublished data). The demonstration by Hinnen et al. (5) that recombinant DNA containing cloned yeast genes can be used to transform yeast cells clearly expands the potential ...
... of the cloned his3 gene that delete DNA sequences near or in the structural gene have been isolated and physically defined (unpublished data). The demonstration by Hinnen et al. (5) that recombinant DNA containing cloned yeast genes can be used to transform yeast cells clearly expands the potential ...
Corchorus yellow vein virus, a New World geminivirus from the Old
... most primer pairs only amplify small fragments of approximately 500 nt in the AV1 gene (Revill et al., 2003; Wyatt & Brown, 1996). To design degenerate primers that would amplify a larger region of DNA A, we aligned begomovirus DNA A sequences from the GenBank database using the CLUSTAL_X program (T ...
... most primer pairs only amplify small fragments of approximately 500 nt in the AV1 gene (Revill et al., 2003; Wyatt & Brown, 1996). To design degenerate primers that would amplify a larger region of DNA A, we aligned begomovirus DNA A sequences from the GenBank database using the CLUSTAL_X program (T ...
chromosomes
... 2) autosomes => two and two fully identical – homologous, pair chromosomes chromosomes of one pair have the same shape, size and the same genes they may not have the same forms of expressing genes– alleles ...
... 2) autosomes => two and two fully identical – homologous, pair chromosomes chromosomes of one pair have the same shape, size and the same genes they may not have the same forms of expressing genes– alleles ...
iGenetics: A Molecular Approach, 3e (Russell/Bose)
... behavioral differences. Yet, all dogs are the same species, and their DNA differs only minutely. Comparison of the genomes of different dog breeds may therefore shed light on which genetic elements are responsible for the radically different developmental pathways of different dog breeds–a process t ...
... behavioral differences. Yet, all dogs are the same species, and their DNA differs only minutely. Comparison of the genomes of different dog breeds may therefore shed light on which genetic elements are responsible for the radically different developmental pathways of different dog breeds–a process t ...
Section 7.1 DNA Cloning with Plasmid Vectors
... such as the genomes of small viruses. But genomes of even the simplest cells are much too large to directly analyze in detail at the molecular level. The problem is compounded for complex organisms. The human genome, for example, contains about 6 × 109 base pairs (bp) in the 23 pairs of chromosomes. ...
... such as the genomes of small viruses. But genomes of even the simplest cells are much too large to directly analyze in detail at the molecular level. The problem is compounded for complex organisms. The human genome, for example, contains about 6 × 109 base pairs (bp) in the 23 pairs of chromosomes. ...
A comprehensive computational model of facilitated diffusion in
... questions consists of building a computational tool able to simulate the relevant molecules in a cell and the entire DNA sequence. This type of approach can address several questions, e.g. how crowding can influence the search process at genome-wide level, in a dynamical context (Chu et al., 2009) a ...
... questions consists of building a computational tool able to simulate the relevant molecules in a cell and the entire DNA sequence. This type of approach can address several questions, e.g. how crowding can influence the search process at genome-wide level, in a dynamical context (Chu et al., 2009) a ...
Detection of aneuploidy in a single cell using the Ion ReproSeq PGS
... aneuploidies in as little material as a single cell, using low amounts of input DNA in a simple, rapid workflow. Independent testing by customers detected 100% of the known copy number changes present in 134 samples. The results were 100% concordant with data obtained from aCGH analysis. However, in ...
... aneuploidies in as little material as a single cell, using low amounts of input DNA in a simple, rapid workflow. Independent testing by customers detected 100% of the known copy number changes present in 134 samples. The results were 100% concordant with data obtained from aCGH analysis. However, in ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.