Case Report Section
... Sample: BM; Culture time: 24h; Banding: G-band. Results: 46,XY,t(3;4)(p21;q34) Other molecular cytogenetic techniques: Fluorescence in situ hybridisation (FISH), with WCP 3 and 4 probes to confirm the t(3;4). To confirm the translocation of 3p and to exclude the translocation t(3;5)(q25;q34-35) FISH ...
... Sample: BM; Culture time: 24h; Banding: G-band. Results: 46,XY,t(3;4)(p21;q34) Other molecular cytogenetic techniques: Fluorescence in situ hybridisation (FISH), with WCP 3 and 4 probes to confirm the t(3;4). To confirm the translocation of 3p and to exclude the translocation t(3;5)(q25;q34-35) FISH ...
Trans-HHS Workshop: Diet, DNA Methylation
... mechanism for the epigenetic control of gene expression and the maintenance of genomic integrity (5,6). Therefore, an evaluation of genomic DNA methylation status is important for the study of cell growth regulation, tissue-specific differentiation (2,4,7) and carcinogenesis (6). Most recently, an i ...
... mechanism for the epigenetic control of gene expression and the maintenance of genomic integrity (5,6). Therefore, an evaluation of genomic DNA methylation status is important for the study of cell growth regulation, tissue-specific differentiation (2,4,7) and carcinogenesis (6). Most recently, an i ...
Reassignment of the Human CSFl Gene to Chromosome lp13-p21
... CSFl gene are responsible for congenital osteopetrosis in up/up mutant mice.*x3 In addition, CSF-1 is produced at high levels by uterine glandular epithelial cells during pregnancy, and may play a role in the formation and differentiation of the p l a ~ e n t a . ~ . ~ Human CSF-1 is encoded by a si ...
... CSFl gene are responsible for congenital osteopetrosis in up/up mutant mice.*x3 In addition, CSF-1 is produced at high levels by uterine glandular epithelial cells during pregnancy, and may play a role in the formation and differentiation of the p l a ~ e n t a . ~ . ~ Human CSF-1 is encoded by a si ...
Unit 10.1.4 - Measuring Genetic Variation using Molecular Markers
... In plant genetic resources conservation, the ultimate aim is to conserve, as far as possible, the broad genetic diversity which is found in the target species. This aim recognises that genetic diversity is a critical component of biodiversity and environmental issues and that the genetic resources t ...
... In plant genetic resources conservation, the ultimate aim is to conserve, as far as possible, the broad genetic diversity which is found in the target species. This aim recognises that genetic diversity is a critical component of biodiversity and environmental issues and that the genetic resources t ...
Mechanical opening of DNA by micromanipulation and force
... complementary oligonucleotides. This way we create two different types of linker arm molecules each having either a digoxygenin or a biotin modified PCR fragment at one extremity and a short nonpalindromic single stranded overhang at the other extremity. The ds-DNA to be opened is a linearized λ-DNA ...
... complementary oligonucleotides. This way we create two different types of linker arm molecules each having either a digoxygenin or a biotin modified PCR fragment at one extremity and a short nonpalindromic single stranded overhang at the other extremity. The ds-DNA to be opened is a linearized λ-DNA ...
Lampbrush Chromosomes of the Chicken
... as the Z chromosome. This chromosome has a striking landmark loop-bearing knob near one end that varies in morphology from a condensed knob to a very extended loop structure (Fig. 2). The opposite end of this chromosome often has a small distinct set of loops. In general, loops on this chromosome se ...
... as the Z chromosome. This chromosome has a striking landmark loop-bearing knob near one end that varies in morphology from a condensed knob to a very extended loop structure (Fig. 2). The opposite end of this chromosome often has a small distinct set of loops. In general, loops on this chromosome se ...
C-Collate3 740..903
... Structures: Visualization.) A giant-loop, random walk model was proposed for interphase chromosome structure based on statistical analysis of the average distance between two chromosome sites, observed in nuclei by FISH, as a function of genomic distance. In this model, 30 nm chromatin ®bers are arr ...
... Structures: Visualization.) A giant-loop, random walk model was proposed for interphase chromosome structure based on statistical analysis of the average distance between two chromosome sites, observed in nuclei by FISH, as a function of genomic distance. In this model, 30 nm chromatin ®bers are arr ...
DNA purification and isolation of genomic DNA from bacterial
... amenable for use in many downstream applications. Although techniques like Southern blotting, which require microgram amounts of DNA, are still performed in molecular biology laboratories, most assessment of chromosomal DNA is done by PCR technology including monoplex or multiplex PCR, SNP analysis ...
... amenable for use in many downstream applications. Although techniques like Southern blotting, which require microgram amounts of DNA, are still performed in molecular biology laboratories, most assessment of chromosomal DNA is done by PCR technology including monoplex or multiplex PCR, SNP analysis ...
Upwelling, Downwelling, and El Nino
... The importance of proper function of repair enzymes is clear from the inherited disorder xeroderma pigmentosum. – These individuals are hypersensitive to sunlight. – In particular, ultraviolet light can produce thymine dimers between adjacent thymine nucleotides. – This buckles the DNA double heli ...
... The importance of proper function of repair enzymes is clear from the inherited disorder xeroderma pigmentosum. – These individuals are hypersensitive to sunlight. – In particular, ultraviolet light can produce thymine dimers between adjacent thymine nucleotides. – This buckles the DNA double heli ...
Powerpoint slides
... cRNA quantitation and process control will help. • A hybrid approach based on global scaling and spikes performs the same as global AD scaling for single designs, and also allows cross-design comparisons ...
... cRNA quantitation and process control will help. • A hybrid approach based on global scaling and spikes performs the same as global AD scaling for single designs, and also allows cross-design comparisons ...
New Developments in Quantitative Real
... system makes use of a novel enzymatic cocktail which does not require the DNA to be cycled between different temperatures, like that done for reactions based on Taq DNA polymerase amplification or any of its variants. In lieu of a standard denaturation step, the HDA system uses the helicase enzyme t ...
... system makes use of a novel enzymatic cocktail which does not require the DNA to be cycled between different temperatures, like that done for reactions based on Taq DNA polymerase amplification or any of its variants. In lieu of a standard denaturation step, the HDA system uses the helicase enzyme t ...
Sequence Enhancer Information - Garvan Institute of Medical
... sulfoxide, and 7-deaza-dGTP in combination on the amplification of three sequences with a high GC content. These molecules have been shown in the past to enhance amplification separately or in combinations of two, such as with 7-deaza-dGTPbetaine or betaine-DMSO. In our hands, the latter combination ...
... sulfoxide, and 7-deaza-dGTP in combination on the amplification of three sequences with a high GC content. These molecules have been shown in the past to enhance amplification separately or in combinations of two, such as with 7-deaza-dGTPbetaine or betaine-DMSO. In our hands, the latter combination ...
Problem Set V - Biology 2970
... chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in such cases the long arm of chromosome 21 has been translocated to another chromoso ...
... chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in such cases the long arm of chromosome 21 has been translocated to another chromoso ...
Promega Enzyme Resource Guide, Cloning Enzymes , BR075B
... RNA ligase. This enzyme does not appear to have any role in nucleic acid metabolism in bacteriophage T4 infected E. coli, but instead appears to be required for the attachment of the bacteriophage’s tail fibers to its base plate during bacteriophage assembly (2). However, its activity as a ligase has ...
... RNA ligase. This enzyme does not appear to have any role in nucleic acid metabolism in bacteriophage T4 infected E. coli, but instead appears to be required for the attachment of the bacteriophage’s tail fibers to its base plate during bacteriophage assembly (2). However, its activity as a ligase has ...
The Structure of the Human AGT Protein Bound to DNA
... The binding of C# by hAGT-A reveals pyrimidine recognition by hAGT. It is known that isolated hAGT can remove alkyl groups from the O4 position of a thymine base.17 Our structure shows that Tyr114 forms a hydrogen bond to O2 of the cytosine, implicating the presence of the same interaction if a dama ...
... The binding of C# by hAGT-A reveals pyrimidine recognition by hAGT. It is known that isolated hAGT can remove alkyl groups from the O4 position of a thymine base.17 Our structure shows that Tyr114 forms a hydrogen bond to O2 of the cytosine, implicating the presence of the same interaction if a dama ...
Electrokinetic Stretching of Tethered DNA
... Shaqfeh, 2003). The observation of single molecules of DNA using fluorescence microscopy has also provided new information on biological processes. For example, several groups have investigated the structure of chromatin fibers, a packaging structure in eukaryotes made up of DNA wound tightly around ...
... Shaqfeh, 2003). The observation of single molecules of DNA using fluorescence microscopy has also provided new information on biological processes. For example, several groups have investigated the structure of chromatin fibers, a packaging structure in eukaryotes made up of DNA wound tightly around ...
Physical Mapping of Important Trait Loci in the Pig
... method of choice. The FISH technique (fluorescence in situ hybridization) makes it possible to assign cloned DNA fragments to specific chromosome regions. A molecular method such as restriction site mapping has been widely used. Pulsed field gel electrophoresis (PFGE) is used to determine the size a ...
... method of choice. The FISH technique (fluorescence in situ hybridization) makes it possible to assign cloned DNA fragments to specific chromosome regions. A molecular method such as restriction site mapping has been widely used. Pulsed field gel electrophoresis (PFGE) is used to determine the size a ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.