Chromosome Analysis Suite 3.1 (ChAS 3.1)
... ChAS 3.1 includes a table with the following calls made for each mutation: “high confidence,”, “lower confidence,” and “undetected.” The thresholds for the “high-confidence” calls were established based on separations between the normal reference and mutant calls that resulted in 95% sensitivity and ...
... ChAS 3.1 includes a table with the following calls made for each mutation: “high confidence,”, “lower confidence,” and “undetected.” The thresholds for the “high-confidence” calls were established based on separations between the normal reference and mutant calls that resulted in 95% sensitivity and ...
LNA-PNA Comparison4
... and DNA:RNA duplexes. The stronger binding is attributed to the lack of charge repulsion between the neutral PNA and the negatively charged DNA or RNA strand and also the PNAs are insensitive to variations in salt concentration and PNA:DNA exhibit minor dependence from 10-50mM sodium chloride. This ...
... and DNA:RNA duplexes. The stronger binding is attributed to the lack of charge repulsion between the neutral PNA and the negatively charged DNA or RNA strand and also the PNAs are insensitive to variations in salt concentration and PNA:DNA exhibit minor dependence from 10-50mM sodium chloride. This ...
Chapter 11 - Chromosome Mutations
... Conclusion: if a recessive mutation appears dominant (pseudodominance) when heterozygous with a deletion, the gene must be included within the deletion ...
... Conclusion: if a recessive mutation appears dominant (pseudodominance) when heterozygous with a deletion, the gene must be included within the deletion ...
1. Introduction
... are dynamic structures, the reconstruction of ancestral karyotypes on the basis of well described extant karyotype is necessary to understand the evolutionary direction of karyotypic variation. The development of complementary techniques for studying chromosomes made it possible to compare karyotype ...
... are dynamic structures, the reconstruction of ancestral karyotypes on the basis of well described extant karyotype is necessary to understand the evolutionary direction of karyotypic variation. The development of complementary techniques for studying chromosomes made it possible to compare karyotype ...
Meiosis - Montville.net
... •This coils to form chromatin fibers. •The chromatin fibers supercoil to form chromosomes that are visible in the metaphase stage of mitosis. ...
... •This coils to form chromatin fibers. •The chromatin fibers supercoil to form chromosomes that are visible in the metaphase stage of mitosis. ...
Minireview Alpha Satellite and the Quest for the Human Centromere
... These include LINEs, Alu repeats, and other satellites (Lee et al., 1997), which are predominantly present in other parts of the genome that never associate with kinetochores, suggesting that they are not by themselves sufficient for centromere function. However, complete sequencing of alpha satelli ...
... These include LINEs, Alu repeats, and other satellites (Lee et al., 1997), which are predominantly present in other parts of the genome that never associate with kinetochores, suggesting that they are not by themselves sufficient for centromere function. However, complete sequencing of alpha satelli ...
Identifying a Knockout Line from Seedpool
... The fluorescence enhancement provided by using the H33258 dye has been shown to be highly specific for DNA, binding preferentially to A-T rich regions (Brunk et al., 1979; Labarca and Paigen, 1980). The dye binds twice as well to double-stranded DNA as to single-stranded DNA, but does not appear to ...
... The fluorescence enhancement provided by using the H33258 dye has been shown to be highly specific for DNA, binding preferentially to A-T rich regions (Brunk et al., 1979; Labarca and Paigen, 1980). The dye binds twice as well to double-stranded DNA as to single-stranded DNA, but does not appear to ...
Presentation
... This assay is based on the principle of alkaline unwinding of strand breaks in double-stranded DNA to yield single-stranded DNA with the number of strand breaks being proportional to the amount of DNA damage. Breaks are monitored by the fluorescence intensity of an intercalating dye, such as Hoechst ...
... This assay is based on the principle of alkaline unwinding of strand breaks in double-stranded DNA to yield single-stranded DNA with the number of strand breaks being proportional to the amount of DNA damage. Breaks are monitored by the fluorescence intensity of an intercalating dye, such as Hoechst ...
Structure and Physiological significance of lipid
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
Etiology : cytogenetics and microdeletions - HAL
... Deletions were found in the 21qter and 14q loci, which are previously annotated in the literature [Kamnasaran and others 2005; Roessler and others 1999], reinforcing their potential involvement in HPE. Several new candidate loci not suspected to be involved in the disease at that time were also iden ...
... Deletions were found in the 21qter and 14q loci, which are previously annotated in the literature [Kamnasaran and others 2005; Roessler and others 1999], reinforcing their potential involvement in HPE. Several new candidate loci not suspected to be involved in the disease at that time were also iden ...
MagJET Plasmid DNA Kit - Thermo Fisher Scientific
... technology for nucleic acid purification. The whole nucleic acid isolation process combines simple steps of sample lysis, DNA binding to the magnetic beads, washing and elution. Pelleted bacterial cells are resuspended and subjected to SDS/alkaline lysis to liberate plasmid DNA. The resulting lysate ...
... technology for nucleic acid purification. The whole nucleic acid isolation process combines simple steps of sample lysis, DNA binding to the magnetic beads, washing and elution. Pelleted bacterial cells are resuspended and subjected to SDS/alkaline lysis to liberate plasmid DNA. The resulting lysate ...
Educational Items Section Chromosomal Disorders - Karyotype Indications in Oncology and Haematology
... - trisomy 21: 1,5/1000; other unbalanced autosomal disorders: 0,5/ 1000; - balanced chromosomal disorders with a normal phenotype 2/1000 - among 1000 known pregnancies 150 will abort spontaneously: among them 100 have an abnormal chromosomal complement: 20 Turner syndrome, 20 trisomies 16 and 20 tri ...
... - trisomy 21: 1,5/1000; other unbalanced autosomal disorders: 0,5/ 1000; - balanced chromosomal disorders with a normal phenotype 2/1000 - among 1000 known pregnancies 150 will abort spontaneously: among them 100 have an abnormal chromosomal complement: 20 Turner syndrome, 20 trisomies 16 and 20 tri ...
Chromosomal mutations
... breakage and rejoining of two or several chromosomes • In balanced translocation there is an equal exchange of chromosomal material Reciprocal translocation: the location of a gene changes, but the amount of genetic material is • Most often either normal or unaltered translocation carrier chromoso ...
... breakage and rejoining of two or several chromosomes • In balanced translocation there is an equal exchange of chromosomal material Reciprocal translocation: the location of a gene changes, but the amount of genetic material is • Most often either normal or unaltered translocation carrier chromoso ...
Slide 1
... There is a pressing need for new rapid molecular methods for identification and sub-typing of Salmonella strains for surveillance, and for analysis of outbreaks. Optical Mapping is a method for producing ordered restriction maps across entire microbial genomes. For a typical 4 million base-pair bact ...
... There is a pressing need for new rapid molecular methods for identification and sub-typing of Salmonella strains for surveillance, and for analysis of outbreaks. Optical Mapping is a method for producing ordered restriction maps across entire microbial genomes. For a typical 4 million base-pair bact ...
DNA Replication and Telomere Maintenance
... • In association with PCNA, DNA ligase I joins the Okazaki fragments by catalyzing the formation of new phosphodiester bonds. • DNA binding domain encircles DNA and interacts with the minor groove. • Stabilizes distorted structure with A-form helix upstream of the gap. ...
... • In association with PCNA, DNA ligase I joins the Okazaki fragments by catalyzing the formation of new phosphodiester bonds. • DNA binding domain encircles DNA and interacts with the minor groove. • Stabilizes distorted structure with A-form helix upstream of the gap. ...
Bacterial Screening PCR Kit
... In order to maintain consumer trust in the safety of food products, a high priority has been placed on assuring product quality at each step of the food supply process. PCR (Polymerase Chain Reaction) process is recognized as one of the more useful methods for such food-related quality control appli ...
... In order to maintain consumer trust in the safety of food products, a high priority has been placed on assuring product quality at each step of the food supply process. PCR (Polymerase Chain Reaction) process is recognized as one of the more useful methods for such food-related quality control appli ...
Modeling Spatial Correlation of DNA Deformation
... model shows a very good agreement between theory and experiment with lP ∼ 150 bps ∼ 50 nm for double-stranded DNA under physiological conditions18 as well as in a flow field.19 Detailed variations of this model have been proposed over the years by introducing a small number of additional independent p ...
... model shows a very good agreement between theory and experiment with lP ∼ 150 bps ∼ 50 nm for double-stranded DNA under physiological conditions18 as well as in a flow field.19 Detailed variations of this model have been proposed over the years by introducing a small number of additional independent p ...
In vitro selection of restriction endonucleases by
... evolution of enzymes. In this system, artificial cell-like compartments provide the linkage of genotype (DNA) and phenotype (protein) for directed protein evolution (8,10,11). So far, IVC has been used for the selection of enzymes such as methyltransferases (12,13), a bacterial phosphotriesterase (1 ...
... evolution of enzymes. In this system, artificial cell-like compartments provide the linkage of genotype (DNA) and phenotype (protein) for directed protein evolution (8,10,11). So far, IVC has been used for the selection of enzymes such as methyltransferases (12,13), a bacterial phosphotriesterase (1 ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.