PLTW Biomedical Competency Profile
... Prepares a written and oral presentation about an assigned disease Analyzes a case study and creates a presentation of findings Creates an evidence board with a team Maintains case notes of an outbreak investigation Compiles a patient case file tracing disease in human systems Research and Developme ...
... Prepares a written and oral presentation about an assigned disease Analyzes a case study and creates a presentation of findings Creates an evidence board with a team Maintains case notes of an outbreak investigation Compiles a patient case file tracing disease in human systems Research and Developme ...
Biotechnology
... a minute or so to denature DNA into single strands 2. Annealing – the mixture is cooled to 50-65 oC and primers attach (by H bonds) to their complementary sequences on either side of the target sequence 3. Elongation – the mixture is brought to 72 oC for several minutes during which polymerase binds ...
... a minute or so to denature DNA into single strands 2. Annealing – the mixture is cooled to 50-65 oC and primers attach (by H bonds) to their complementary sequences on either side of the target sequence 3. Elongation – the mixture is brought to 72 oC for several minutes during which polymerase binds ...
Lecture 4, Exam III Worksheet Answers
... 1. What is the purpose of telomeres? What type of cells are they most important within? What types of cells are they least important in? What enzyme creates telomeres and how? What is special about the enzyme that allows it to carry out its function? There is a portion of a cell’s complementary DNA ...
... 1. What is the purpose of telomeres? What type of cells are they most important within? What types of cells are they least important in? What enzyme creates telomeres and how? What is special about the enzyme that allows it to carry out its function? There is a portion of a cell’s complementary DNA ...
Students Visit DNA Learning Center
... fosters development of creative and critical thinking skills, while increasing facility with scientific concepts. Labs are held in the afternoon in the Learning Center's Bio2000 Teaching Laboratory. Each lab is approximately 90 minutes. Variability and Inheritance Fruit flies in a mixture are sorted ...
... fosters development of creative and critical thinking skills, while increasing facility with scientific concepts. Labs are held in the afternoon in the Learning Center's Bio2000 Teaching Laboratory. Each lab is approximately 90 minutes. Variability and Inheritance Fruit flies in a mixture are sorted ...
HiPerDART Targets and Objectives
... predicting the prognosis of stage II and III colon cancer (Eschrich et al, 2005; Barrier et al, 2006; Lin et al, 2007) but need to be validated before being routinely used, which is jeopardized by the current cost of assessing the large number of genes in these profiles. In this project we aim to de ...
... predicting the prognosis of stage II and III colon cancer (Eschrich et al, 2005; Barrier et al, 2006; Lin et al, 2007) but need to be validated before being routinely used, which is jeopardized by the current cost of assessing the large number of genes in these profiles. In this project we aim to de ...
Genetic Control of Cell Function and Inheritance
... Autosomal Dominant Disorders • Single mutant gene from an affected parent is transmitted to an offspring regardless of sex • The affected parent has a 50% chance of transmitting the disorder to each offspring • Unaffected siblings of the offspring do not transmit the disorder • In some cases, the p ...
... Autosomal Dominant Disorders • Single mutant gene from an affected parent is transmitted to an offspring regardless of sex • The affected parent has a 50% chance of transmitting the disorder to each offspring • Unaffected siblings of the offspring do not transmit the disorder • In some cases, the p ...
8-3 Notes with Power point
... 5. Another DNA Polymerase also “proofreads” the new DNA to check for errors. 6. Meanwhile, on the other strand, known as the _________________________, Primase and DNA Polymerase synthesize DNA from 5’ to 3’ away from the replication fork. These small spurts of replication form what are known as ___ ...
... 5. Another DNA Polymerase also “proofreads” the new DNA to check for errors. 6. Meanwhile, on the other strand, known as the _________________________, Primase and DNA Polymerase synthesize DNA from 5’ to 3’ away from the replication fork. These small spurts of replication form what are known as ___ ...
Genealogy: To DNA or not to DNA?
... 2. Mitochondrial DNA (mtDNA) is passed down intact through the female line of a family. Both men and women inherit their mtDNA from their mothers, but only women can pass it on to the next generation. When two people share the same mtDNA they will have a common ancestor along the female line of thei ...
... 2. Mitochondrial DNA (mtDNA) is passed down intact through the female line of a family. Both men and women inherit their mtDNA from their mothers, but only women can pass it on to the next generation. When two people share the same mtDNA they will have a common ancestor along the female line of thei ...
R 9.1
... Name: _____________________________ Class: __________________ Date: __________________ ...
... Name: _____________________________ Class: __________________ Date: __________________ ...
Brooker Chapter 9
... – Bacteria with single circular chromosome without a nucleus (prokaryotes) – Bacteriophage (“bacteria eaters”) ...
... – Bacteria with single circular chromosome without a nucleus (prokaryotes) – Bacteriophage (“bacteria eaters”) ...
Supplementary Information (doc 59K)
... lysate was transferred to a new tube without carrying over any cell debris. DNA was precipitated with 200µl Isopropanol at -20°C over night. The DNA pellet was washed with 500µl 70% Ethanol, air dried and dissolved in 20µl TE buffer. Methylation analysis DNA derived from tumor samples is mainly of l ...
... lysate was transferred to a new tube without carrying over any cell debris. DNA was precipitated with 200µl Isopropanol at -20°C over night. The DNA pellet was washed with 500µl 70% Ethanol, air dried and dissolved in 20µl TE buffer. Methylation analysis DNA derived from tumor samples is mainly of l ...
direct genetic testing
... Number of multiple loci probes (MLP) identified Core sequence GGAGGTGGGCAGGA 2 of these used (33.15 and 33.6) hybridised to Southern Blots of restriction-digested genomic DNA Shared ‘core’ sequences at multiple loci creates hypervariable, multi-band patterns called DNA ‘fingerprints Together, upto 3 ...
... Number of multiple loci probes (MLP) identified Core sequence GGAGGTGGGCAGGA 2 of these used (33.15 and 33.6) hybridised to Southern Blots of restriction-digested genomic DNA Shared ‘core’ sequences at multiple loci creates hypervariable, multi-band patterns called DNA ‘fingerprints Together, upto 3 ...
DNA Unit Test Study Guide extra added
... protein. It is the site of where the proteins are built or synthesized. 10. Mutations: 3 types, effects of mutations A. Substitution – One base is replaced by another – this is the most common mutation and is the least harmful B. Insertion – An extra base is added C. Deletion – A base is left out Th ...
... protein. It is the site of where the proteins are built or synthesized. 10. Mutations: 3 types, effects of mutations A. Substitution – One base is replaced by another – this is the most common mutation and is the least harmful B. Insertion – An extra base is added C. Deletion – A base is left out Th ...
Grimmer presentation
... • Obtaining Genomic Data • Whole genome sequencing • Human genotyping microarrays • Targeted re-sequencing (Targeted DNA Capture) • Gene regions identified by Genome Wide Association studies (GWAs) ...
... • Obtaining Genomic Data • Whole genome sequencing • Human genotyping microarrays • Targeted re-sequencing (Targeted DNA Capture) • Gene regions identified by Genome Wide Association studies (GWAs) ...
Brooker Chapter 9
... – Bacteria with single circular chromosome without a nucleus (prokaryotes) – Bacteriophage (“bacteria eaters”) ...
... – Bacteria with single circular chromosome without a nucleus (prokaryotes) – Bacteriophage (“bacteria eaters”) ...
The Genetic Material
... – Bacteria with single circular chromosome without a nucleus (prokaryotes) – Bacteriophage (“bacteria eaters”) ...
... – Bacteria with single circular chromosome without a nucleus (prokaryotes) – Bacteriophage (“bacteria eaters”) ...
Trends in Biotechnology
... 5. List the types of vectors that can be used to transform yeast, mammalian cells and plants, and why they are effective in those organisms. 6. List the methods of transformation of cells. ...
... 5. List the types of vectors that can be used to transform yeast, mammalian cells and plants, and why they are effective in those organisms. 6. List the methods of transformation of cells. ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.