DNA Fingerprinting and Forensic Analysis - ASAB-NUST
... • Infrared light is used for this job, because blood vessel on the retina absorb this better than the surrounding tissue • A computer algorithm is used to convert this scan into digital data • There are about 10 fold more information on retinal scan than in fingerprints ...
... • Infrared light is used for this job, because blood vessel on the retina absorb this better than the surrounding tissue • A computer algorithm is used to convert this scan into digital data • There are about 10 fold more information on retinal scan than in fingerprints ...
View PDF of poster here
... Rapid lysis of bacterial cells and fragmentation of bacterial genomic DNA is carried out in a lysing chamber (Figure 3A) using conventional microwave irradiation. The lysing chambers are composed of gold triangles deposited on glass slides, and a self-adhesive silicon isolators (D = 30 mm) placed ov ...
... Rapid lysis of bacterial cells and fragmentation of bacterial genomic DNA is carried out in a lysing chamber (Figure 3A) using conventional microwave irradiation. The lysing chambers are composed of gold triangles deposited on glass slides, and a self-adhesive silicon isolators (D = 30 mm) placed ov ...
Restriction Mapping Restriction Fragment Length Polymorphism
... These are 1 to 5 kb in length consisting of repeats 15 to 100 nucleotides in length and are identified by Southern analysis. 2. Microsatellite DNA ...
... These are 1 to 5 kb in length consisting of repeats 15 to 100 nucleotides in length and are identified by Southern analysis. 2. Microsatellite DNA ...
Studying and Manipulating Genomes
... fragmented DNA placed on gel • Fragments move off gel in size order; pass through laser beam • Color each fragment fluoresces is recorded on printout ...
... fragmented DNA placed on gel • Fragments move off gel in size order; pass through laser beam • Color each fragment fluoresces is recorded on printout ...
Chapter 19 – Molecular Genetic Analysis and Biotechnology
... • Helps with linkage studies for gene mapping • DNA fingerprinting – Also uses microsatellites – short tandem repeats • Size of fragment depends on number of repeats ...
... • Helps with linkage studies for gene mapping • DNA fingerprinting – Also uses microsatellites – short tandem repeats • Size of fragment depends on number of repeats ...
BioRad #166-0007EDU: Forensic DNA Fingerprinting Checklist PREP
... (restriction sites). Restriction endonucleases are named for the bacteria from which they were isolated. For example, EcoRI was isolated from Escherichia coli. Endonucleases slide along the DNA helix until it recognizes specific base pairs that signal it to stop. The enzyme then cuts the DNA at that ...
... (restriction sites). Restriction endonucleases are named for the bacteria from which they were isolated. For example, EcoRI was isolated from Escherichia coli. Endonucleases slide along the DNA helix until it recognizes specific base pairs that signal it to stop. The enzyme then cuts the DNA at that ...
DNA
... • 1944- Avery identified DNA as the transforming factor • 1952- Hershey and Chase confirmed Avery’s results by radioactive tagging ...
... • 1944- Avery identified DNA as the transforming factor • 1952- Hershey and Chase confirmed Avery’s results by radioactive tagging ...
Genetic Engineering
... Electrophoresis 1. “Cut” DNA sample with restriction enzymes. 2. Run the DNA fragments through a gel. ...
... Electrophoresis 1. “Cut” DNA sample with restriction enzymes. 2. Run the DNA fragments through a gel. ...
Tandem repeats - Trimble County Schools
... What are tandem repeats and how are they used in forensic science? What are the dominant DNA typing procedures in the US until the mid 1990s? What is short tandem repeat and why is it so attractive to forensic scientists? How does the number of STRs characterized relate to the frequency of occurrenc ...
... What are tandem repeats and how are they used in forensic science? What are the dominant DNA typing procedures in the US until the mid 1990s? What is short tandem repeat and why is it so attractive to forensic scientists? How does the number of STRs characterized relate to the frequency of occurrenc ...
13.2 Notes - Trimble County Schools
... • Nylon membrane is treated with radioactive probes containing one base sequence complementary to the RFLP (hybridization) • Nylon sheet is place against X ray film, exposed and processed which causes bands to appear which can be compared to test specimens ...
... • Nylon membrane is treated with radioactive probes containing one base sequence complementary to the RFLP (hybridization) • Nylon sheet is place against X ray film, exposed and processed which causes bands to appear which can be compared to test specimens ...
Section 2
... which determine the characteristics of an organism and the instructions each cell must follow. A short section of DNA with instructions to make a specific protein is called a _GENE_. The sum of these for an organism is called its GENOME. ...
... which determine the characteristics of an organism and the instructions each cell must follow. A short section of DNA with instructions to make a specific protein is called a _GENE_. The sum of these for an organism is called its GENOME. ...
DNA notes
... Outline: Key points on DNA Structure and Replication A) DNA is a double helix molecule • The two strands are held together by hydrogen bonds between the base pairs A=T (2 bonds) and G=C (3 bonds) • The base pairs, like the steps on a spiral staircase, extend in to the center of the molecule • The "f ...
... Outline: Key points on DNA Structure and Replication A) DNA is a double helix molecule • The two strands are held together by hydrogen bonds between the base pairs A=T (2 bonds) and G=C (3 bonds) • The base pairs, like the steps on a spiral staircase, extend in to the center of the molecule • The "f ...
Biotech Overview
... A faster method of amplifying or making more copies of a gene is PCR PCR requires short pieces of single-stranded DNA which match up to a regions at the beginning & end of the gene to be amplified, called primers Primers are required as a starting point for the DNA polymerase, the same enzyme used i ...
... A faster method of amplifying or making more copies of a gene is PCR PCR requires short pieces of single-stranded DNA which match up to a regions at the beginning & end of the gene to be amplified, called primers Primers are required as a starting point for the DNA polymerase, the same enzyme used i ...
Molecular Genetics
... GOI = gene of interest Vector = method of introducing foreign DNA into cell (usually plasmid) Transformation - uptake of vector containing GOI ...
... GOI = gene of interest Vector = method of introducing foreign DNA into cell (usually plasmid) Transformation - uptake of vector containing GOI ...
Biology 303 EXAM II 3/14/00 NAME
... Which one of the following is part of the Watson-Crick model for DNA? 1. DNA is triple-stranded. 2. the DNA helix is left-handed. 3. DNA consists of two strands of deoxynucleotides with the same polarity. 4. the number of purines equals the number of pyrimidines. ...
... Which one of the following is part of the Watson-Crick model for DNA? 1. DNA is triple-stranded. 2. the DNA helix is left-handed. 3. DNA consists of two strands of deoxynucleotides with the same polarity. 4. the number of purines equals the number of pyrimidines. ...
Sample Exam II
... 1. two genes on the same chromosome can never assort independently from one another. 2. two genes on different chromosomes will likely display linkage. 3. two genes on the same chromosome will always appear to be genetically linked to one another in a dihybrid cross. 4. if two genes are genetically ...
... 1. two genes on the same chromosome can never assort independently from one another. 2. two genes on different chromosomes will likely display linkage. 3. two genes on the same chromosome will always appear to be genetically linked to one another in a dihybrid cross. 4. if two genes are genetically ...
Genetic Technology
... Genetic Engineering • Under the genetic engineering flap write the following definitions and examples • Method of cutting DNA from one organism and inserting the DNA fragments into a host organism of the same or different species. • Scientist use Ecoli bacteria to make expensive Die for blue Jeans ...
... Genetic Engineering • Under the genetic engineering flap write the following definitions and examples • Method of cutting DNA from one organism and inserting the DNA fragments into a host organism of the same or different species. • Scientist use Ecoli bacteria to make expensive Die for blue Jeans ...
Team Uses PacBio Data to Detect and Phase Bacterial DNA
... Comparing IPD values from native DNA with IPD values from whole-genome amplified DNA, which has lost its methylation, the researchers calculated a score that allowed them to detect methylation at specific sequence motifs within a single DNA molecule. The second method uses long-insert libraries of a ...
... Comparing IPD values from native DNA with IPD values from whole-genome amplified DNA, which has lost its methylation, the researchers calculated a score that allowed them to detect methylation at specific sequence motifs within a single DNA molecule. The second method uses long-insert libraries of a ...
Name: Date: Period:_____ Midterm Review: Study Guide # 3
... 2. Next, scan the objectives for the topic you are about to study in order to get a sense of what you should be focusing your time and energy on. 3. Start mastering each objective by answering the associated review questions right on this sheet. 4. After you have finished, use this sheet as a study ...
... 2. Next, scan the objectives for the topic you are about to study in order to get a sense of what you should be focusing your time and energy on. 3. Start mastering each objective by answering the associated review questions right on this sheet. 4. After you have finished, use this sheet as a study ...
Fields of Fingerprints Text Passage – 9th Grade
... carries the trait. At one time, the researcher would have to grow the crop to see if the trait is present. But now, the DNA of the seed batch can be tested to determine if the seeds contain the sought-after gene. The DNA test can also be used to identify and keep track of genes as they are isolated ...
... carries the trait. At one time, the researcher would have to grow the crop to see if the trait is present. But now, the DNA of the seed batch can be tested to determine if the seeds contain the sought-after gene. The DNA test can also be used to identify and keep track of genes as they are isolated ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.