Microarray Technology in Practice Brochure
... corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain d ...
... corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain d ...
交通大學特色研究計畫邀請 - 國立交通大學生物資訊研究所
... These topological domains may be formed through constraining each DNA end from rotating by interacting with nuclear proteins, i.e., DNA-binding proteins. However, so far, evidence to support this hypothesis is still elusive. Here we developed two biochemical methods, i.e., DNA-nicking and DNA-gyrase ...
... These topological domains may be formed through constraining each DNA end from rotating by interacting with nuclear proteins, i.e., DNA-binding proteins. However, so far, evidence to support this hypothesis is still elusive. Here we developed two biochemical methods, i.e., DNA-nicking and DNA-gyrase ...
DNA switches
... The findings have immediate applications for understanding how alterations in the nongene parts of DNA contribute to human diseases, which may in turn lead to new drugs. They can also help explain how the environment can affect disease risk. In the case of identical twins, small changes in environm ...
... The findings have immediate applications for understanding how alterations in the nongene parts of DNA contribute to human diseases, which may in turn lead to new drugs. They can also help explain how the environment can affect disease risk. In the case of identical twins, small changes in environm ...
COA: phiX174 DNA/BsuRI (HaeIII) Marker, 9, ready-to
... deoxyribonucleic acid restriction fragments on polyacrylamide gels, Biochemistry, 22, 6186-6193, 1983. 2. Lane, D., et al., Use of gel ratardation to analyze protein – nucleic acid interactions, Microbiological Reviews, 56, 509528, 1992. 3. Stellwagen, N.C., Conformational isomers of curved DNA mole ...
... deoxyribonucleic acid restriction fragments on polyacrylamide gels, Biochemistry, 22, 6186-6193, 1983. 2. Lane, D., et al., Use of gel ratardation to analyze protein – nucleic acid interactions, Microbiological Reviews, 56, 509528, 1992. 3. Stellwagen, N.C., Conformational isomers of curved DNA mole ...
UV-Induced DNA Damage and Repair
... action of sunlight to be primarily attributable to the UV portion of the spectrum near 260 nm. This corresponds to the Amax for the DNA bases, whereas the Amax for proteins is near 280 nm. UV irradiation is a widely used a method for decontamination by "germicidal lamps". UV-induced mutagenicity (as ...
... action of sunlight to be primarily attributable to the UV portion of the spectrum near 260 nm. This corresponds to the Amax for the DNA bases, whereas the Amax for proteins is near 280 nm. UV irradiation is a widely used a method for decontamination by "germicidal lamps". UV-induced mutagenicity (as ...
Electric Field Effect Detection of Biomolecular Interactions P. Estrela
... metal (probe molecules) are exposed to their bioconjugates (target molecules). The ability to detect such reactions is essential in fields such as genomics and proteomics. One commonly employed method is the optical detection, which employs labeling of the targets with fluorescent tags and expensive ...
... metal (probe molecules) are exposed to their bioconjugates (target molecules). The ability to detect such reactions is essential in fields such as genomics and proteomics. One commonly employed method is the optical detection, which employs labeling of the targets with fluorescent tags and expensive ...
DNA Fingerprinting: A Powerful Law-Enforcement Tool with Serious
... was coined by geneticist Alec J. Jeffreys, University of Leicester, U. K., who developed the method in 1985. Several of Jeffreys’ “hot” papers were identified in a recent issue of The Scientist (January 23, 1989, p.12). Briefly described, Jeffreys’ method produces a complex pattern of bands from par ...
... was coined by geneticist Alec J. Jeffreys, University of Leicester, U. K., who developed the method in 1985. Several of Jeffreys’ “hot” papers were identified in a recent issue of The Scientist (January 23, 1989, p.12). Briefly described, Jeffreys’ method produces a complex pattern of bands from par ...
180-183
... He called the process transformation because one type of bacteria (a harmless form) had been changed permanently into another (a disease-carrying form). Because the ability to cause disease was inherited by the offspring of the transformed bacteria, he concluded that the transforming factor had to b ...
... He called the process transformation because one type of bacteria (a harmless form) had been changed permanently into another (a disease-carrying form). Because the ability to cause disease was inherited by the offspring of the transformed bacteria, he concluded that the transforming factor had to b ...
Genetic engineering and biotechnology techniques
... genome mapping before cheaper methods such as PCR (polymerase chain reaction) and DNA sequencing came along ...
... genome mapping before cheaper methods such as PCR (polymerase chain reaction) and DNA sequencing came along ...
File
... DNA Fingerprinting Activity Introduction: DNA fingerprinting relies on the fact that the DNA code is universal for all living things and that there are differences between individuals within that code. Because human DNA is very similar to every other human’s DNA, DNA fingerprinting primarily focuses ...
... DNA Fingerprinting Activity Introduction: DNA fingerprinting relies on the fact that the DNA code is universal for all living things and that there are differences between individuals within that code. Because human DNA is very similar to every other human’s DNA, DNA fingerprinting primarily focuses ...
DNA EXTRACTION
... base. There are four different bases: adenine (A), guanine (G), cytosine (C) and thymine (T). The essential information in the genome of the cell is coded by those four bases. Prokaryotes (such as bacteria) have their DNA in the cytosol (cytoplasm). Eukaryotes (such as animals, plants and fungi) sto ...
... base. There are four different bases: adenine (A), guanine (G), cytosine (C) and thymine (T). The essential information in the genome of the cell is coded by those four bases. Prokaryotes (such as bacteria) have their DNA in the cytosol (cytoplasm). Eukaryotes (such as animals, plants and fungi) sto ...
From DNA to Protein Name: What does DNA stand for? What is DNA
... 12. If the sequence of nucleotides on the original DNA strand was A-G-G-C-T-A, what would the nucleotide sequence on the complementary strand of DNA? ...
... 12. If the sequence of nucleotides on the original DNA strand was A-G-G-C-T-A, what would the nucleotide sequence on the complementary strand of DNA? ...
- human genetics
... The process of DNA fingerprinting is based on the fact that a. the most important genes are different among most people. b. no two people, except identical twins, have exactly the same DNA. c. most genes are dominant. d. most people have DNA that contains repeats. What conclusion CANNOT be made h m ...
... The process of DNA fingerprinting is based on the fact that a. the most important genes are different among most people. b. no two people, except identical twins, have exactly the same DNA. c. most genes are dominant. d. most people have DNA that contains repeats. What conclusion CANNOT be made h m ...
THE CELLULAR AND MOLECULAR BASIS OF INHERITANCE
... • The backbone of each chain is formed by phosphodiester bonds between the 3' and 5' carbons of adjacent sugars. • The two chains being held together by hydrogen bonds between the nitrogenous bases which point in towards the centre of the helix. • The two DNA strands are held together by hydrogen bo ...
... • The backbone of each chain is formed by phosphodiester bonds between the 3' and 5' carbons of adjacent sugars. • The two chains being held together by hydrogen bonds between the nitrogenous bases which point in towards the centre of the helix. • The two DNA strands are held together by hydrogen bo ...
Ch 020 DNA Technology II
... basis of size or electrical charge creating DNA bands of the same length ...
... basis of size or electrical charge creating DNA bands of the same length ...
genes: genetics, gemonics, an evolution
... ____ 27. One of the first successful applications of genetic engineering was the commercial production of a. clotting factor. b. insulin. c. hemoglobin. d. collagen. e. human growth factor. ____ 28. Seed banks a. provide a source of seeds for farmers. b. preserve seeds for museums. c. preserve plan ...
... ____ 27. One of the first successful applications of genetic engineering was the commercial production of a. clotting factor. b. insulin. c. hemoglobin. d. collagen. e. human growth factor. ____ 28. Seed banks a. provide a source of seeds for farmers. b. preserve seeds for museums. c. preserve plan ...
Human karyotype
... DNA is packaged into chromosomes • Each human cell contains 2 metres of DNA (3,000,000,000 bases in a haploid cell) • Nucleus is 5 microns (0.005 mm) diameter • DNA must be properly packaged, not just tangled up and stuffed into nucleus • Packaging involves coiling and folding the DNA in specific w ...
... DNA is packaged into chromosomes • Each human cell contains 2 metres of DNA (3,000,000,000 bases in a haploid cell) • Nucleus is 5 microns (0.005 mm) diameter • DNA must be properly packaged, not just tangled up and stuffed into nucleus • Packaging involves coiling and folding the DNA in specific w ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.