SEGMENTAL VARIATION
... Separating the wheat from the chaff • Technical artifacts (ligation of unrelated fragments during library preparation) may be numerous but will be random • Artifacts related to homologous sequences (see previous slide) will be reproducible but common to all samples • Real structural variants will be ...
... Separating the wheat from the chaff • Technical artifacts (ligation of unrelated fragments during library preparation) may be numerous but will be random • Artifacts related to homologous sequences (see previous slide) will be reproducible but common to all samples • Real structural variants will be ...
Key concepts_chromatin
... The genomes of all organisms are compacted by interaction with specific proteins. These often play roles in gene regulation. In bacteria, a large circular chromosome is complexed by proteins in a dynamic manner that compacts it and yet makes it accessible for transcription. Eukaryotes utilize a set ...
... The genomes of all organisms are compacted by interaction with specific proteins. These often play roles in gene regulation. In bacteria, a large circular chromosome is complexed by proteins in a dynamic manner that compacts it and yet makes it accessible for transcription. Eukaryotes utilize a set ...
The History of DNA WebQuest
... history of DNA. You decide to research the following: • The scientists involved in the discovery of DNA. • The discoveries and research that led to the realization that DNA was the genetic material. • The scientists who were involved in discovering the structure of DNA. ...
... history of DNA. You decide to research the following: • The scientists involved in the discovery of DNA. • The discoveries and research that led to the realization that DNA was the genetic material. • The scientists who were involved in discovering the structure of DNA. ...
Case study - DNA Genotek
... samples. Sixteen months after launching the program, a majority of samples, greater than 52%, are being collected with Performagene. The high compliance can be attributed to the ease of use, fast and non-invasive nature of the collection device which makes the process intuitive for producers. Perfor ...
... samples. Sixteen months after launching the program, a majority of samples, greater than 52%, are being collected with Performagene. The high compliance can be attributed to the ease of use, fast and non-invasive nature of the collection device which makes the process intuitive for producers. Perfor ...
Biotech quiz review
... bacteria cells – what would be their first step? Use a restriction enzyme to cut out the human gene and the plasmid ...
... bacteria cells – what would be their first step? Use a restriction enzyme to cut out the human gene and the plasmid ...
DNA Word Messages
... 3. What is the process of transcription? 4. What location does transcription occur? 5. What is the process of translation? 6. What location does translation occur? 7. Each mRNA has a cap and poly-A-tail. What is their purpose? 8. Compare and contrast DNA polymerase and RNA polymerase? 9. Does transc ...
... 3. What is the process of transcription? 4. What location does transcription occur? 5. What is the process of translation? 6. What location does translation occur? 7. Each mRNA has a cap and poly-A-tail. What is their purpose? 8. Compare and contrast DNA polymerase and RNA polymerase? 9. Does transc ...
Ch 13 Genetic Engineering
... 13.2 Manipulating DNA • DNA Extraction- DNA is chemically removed from cells • Cutting DNA- Hundreds of Restriction Enzymes are known and each one cuts DNA at a specific sequence of nucleotides • Separating DNA- A mixture of DNA fragments is placed at one end of a porous gel and an electrical charg ...
... 13.2 Manipulating DNA • DNA Extraction- DNA is chemically removed from cells • Cutting DNA- Hundreds of Restriction Enzymes are known and each one cuts DNA at a specific sequence of nucleotides • Separating DNA- A mixture of DNA fragments is placed at one end of a porous gel and an electrical charg ...
News Release
... While Eddie clearly has many ancestors, if we want to trace a family line back through the generations, there are two ancestral lineages that we can learn much more about than the others, that of the father’s father’s father and the mother’s mother’s mother and so on back in time. The fatherline is ...
... While Eddie clearly has many ancestors, if we want to trace a family line back through the generations, there are two ancestral lineages that we can learn much more about than the others, that of the father’s father’s father and the mother’s mother’s mother and so on back in time. The fatherline is ...
DNA Fingerprinting – Your Bioremediation “Taq”ometer
... ¾ We are a wholly-owned subsidiary of Golder Associates Ltd. ¾ Our talented team of professionals all have degrees and expertise in the natural sciences (microbiology, biochemistry, toxicology, ecology, botany and zoology). ...
... ¾ We are a wholly-owned subsidiary of Golder Associates Ltd. ¾ Our talented team of professionals all have degrees and expertise in the natural sciences (microbiology, biochemistry, toxicology, ecology, botany and zoology). ...
Aim: What is the structure of the DNA molecule?
... DNA is a special molecule found in the cells which make up a chromosome. Chromosomes are found in the nucleus of a cell. (Therefore DNA is in the nucleus) There are 46 pairs of chromosomes in the human cell. DNA is an instruction manual for all the processes that the organism does. DNA has all the i ...
... DNA is a special molecule found in the cells which make up a chromosome. Chromosomes are found in the nucleus of a cell. (Therefore DNA is in the nucleus) There are 46 pairs of chromosomes in the human cell. DNA is an instruction manual for all the processes that the organism does. DNA has all the i ...
cell division notes -
... microscope images of DNA more images, including a gallery of the X-ray diffraction images taken by Franklin and used by Watson and Crick in their solution to the structure of DNA 1. double helix – discovered by Watson and Crick; image taken by Franklin – p. 186 fig. 10.3 ...
... microscope images of DNA more images, including a gallery of the X-ray diffraction images taken by Franklin and used by Watson and Crick in their solution to the structure of DNA 1. double helix – discovered by Watson and Crick; image taken by Franklin – p. 186 fig. 10.3 ...
Genetic Diseases and Gene Therapy
... • What are the differences between cloning, recombinant DNA, and genetic engineering? • What are the tools we use for genetic engineering? – Plasmids – Restriction Enzymes – DNA Ligase ...
... • What are the differences between cloning, recombinant DNA, and genetic engineering? • What are the tools we use for genetic engineering? – Plasmids – Restriction Enzymes – DNA Ligase ...
dna technology and genomics
... 2) Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3) Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 4) Describe techniques that allow identification of recombinant ce ...
... 2) Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3) Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 4) Describe techniques that allow identification of recombinant ce ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.