Genetic Engineering
... Cutting the DNA – using restrictive enzymes to break apart DNA at a specific locations they can get the piece they want Separating DNA – using electrophoresis to separate the DNA fragments to study Pasting – using enzymes to put together the desired sequence ...
... Cutting the DNA – using restrictive enzymes to break apart DNA at a specific locations they can get the piece they want Separating DNA – using electrophoresis to separate the DNA fragments to study Pasting – using enzymes to put together the desired sequence ...
Genetic Engineering
... Genomic Libraries – (total DNA of a cell is referred to as a genome) • genomic library – a collection of DNA fragments that are more or less representative of all the DNA in the genome • each fragment is spliced into a plasmid, which is usually contained inside a bacterial cell • restriction enzym ...
... Genomic Libraries – (total DNA of a cell is referred to as a genome) • genomic library – a collection of DNA fragments that are more or less representative of all the DNA in the genome • each fragment is spliced into a plasmid, which is usually contained inside a bacterial cell • restriction enzym ...
SBI4U MG Restriction Enzymes
... How are RE named?! ! RE are named after the bacteria from which they were isolated.! ! Example: EcoRI E! !Escherichia (genus)! co !coli (species)! R! !RY13 (strain)! I ! !first endonuclease isolated! ...
... How are RE named?! ! RE are named after the bacteria from which they were isolated.! ! Example: EcoRI E! !Escherichia (genus)! co !coli (species)! R! !RY13 (strain)! I ! !first endonuclease isolated! ...
Mrs. Paparella/ Living Environment Genetics Essential Questions
... 19. What is done in genetic engineering and what are 2 examples of its use? Genetic engineering involves the cutting out of a gene from one organism (using restriction enzymes) and inserting it into another organism’s DNA (with the help of enzymes) so that the second organism ( usually a bacteria) c ...
... 19. What is done in genetic engineering and what are 2 examples of its use? Genetic engineering involves the cutting out of a gene from one organism (using restriction enzymes) and inserting it into another organism’s DNA (with the help of enzymes) so that the second organism ( usually a bacteria) c ...
GM skills - KingsfieldBiology
... • Plasmids can be cut open with restriction endonucleases • If a gene is cut out with the same enzyme they will have complementary sticky ends • DNA ligase seals up the gap in between by forming a phosphodiester bond ...
... • Plasmids can be cut open with restriction endonucleases • If a gene is cut out with the same enzyme they will have complementary sticky ends • DNA ligase seals up the gap in between by forming a phosphodiester bond ...
review WS
... 8. Avery and Colleagues 9. Hershey and Chase – What did they do? How did they label the DNA? Protein? 10. What is Chargaff’s Rule 11. Differentiate between a purine and a pyrimidine. 12. What is DNA replication 13. What is meant by semi-conservative replication? 14. How are the two new DNA molecules ...
... 8. Avery and Colleagues 9. Hershey and Chase – What did they do? How did they label the DNA? Protein? 10. What is Chargaff’s Rule 11. Differentiate between a purine and a pyrimidine. 12. What is DNA replication 13. What is meant by semi-conservative replication? 14. How are the two new DNA molecules ...
Genetics Study Guide
... What are the 4 nucleotides that make up DNA? What does DNA stand for? Who discovered that DNA is in the form of a double helix? Who is the father of modern genetics, he discovered that you inherit one gene from each parent? Who developed a fingerprint classification system? Who discovered that DNA c ...
... What are the 4 nucleotides that make up DNA? What does DNA stand for? Who discovered that DNA is in the form of a double helix? Who is the father of modern genetics, he discovered that you inherit one gene from each parent? Who developed a fingerprint classification system? Who discovered that DNA c ...
What would we like to know about DNA and how do we obtain that
... • Sequencing an organisms entire genome • Why would we want to do this? ...
... • Sequencing an organisms entire genome • Why would we want to do this? ...
DNA and Protein Synthesis Concept Questions
... 4. Why is DNA replication important for every cell? 5. Compare the amount of DNA in a muscle cell with that in a brain cell. 6. a) Why is the making of exact copies of DNA called replication rather than duplication? b) What is meant by saying that DNA replication is semi-conservative? 7. Why is repl ...
... 4. Why is DNA replication important for every cell? 5. Compare the amount of DNA in a muscle cell with that in a brain cell. 6. a) Why is the making of exact copies of DNA called replication rather than duplication? b) What is meant by saying that DNA replication is semi-conservative? 7. Why is repl ...
Ch 20- Mini Clicker Review Qs
... Pieces of DNA that are run through a gel in order to give a unique banding pattern Single-stranded DNA ends that are available to hydrogen bond to a complimentary single strand DNA bases that are added to a PCR machine so that multiple exact copies of a DNA sequence can be produced Segments of DNA t ...
... Pieces of DNA that are run through a gel in order to give a unique banding pattern Single-stranded DNA ends that are available to hydrogen bond to a complimentary single strand DNA bases that are added to a PCR machine so that multiple exact copies of a DNA sequence can be produced Segments of DNA t ...
Eucharyotic Chromatin Organization
... Why is the control of gene expression more complex in eukaryotes than prokaryotes ? (2) 4) cells that require cell specialization or ...
... Why is the control of gene expression more complex in eukaryotes than prokaryotes ? (2) 4) cells that require cell specialization or ...
Southern hybridization
... encoded by the sequence of the nucleotide bases in DNA of the gene. The four nucleotides are: adenine (A), thymine (T), guanine (G), and cytosine (C), a mutation is a change in the order of these ...
... encoded by the sequence of the nucleotide bases in DNA of the gene. The four nucleotides are: adenine (A), thymine (T), guanine (G), and cytosine (C), a mutation is a change in the order of these ...
DNA - The Double Helix
... the production of proteins within the cell. These proteins in turn, form the structural units of cells and control all chemical processes within the cell. Think of proteins as the building blocks for an organism, proteins make up your skin, your hair, parts of individual cells. How you look is large ...
... the production of proteins within the cell. These proteins in turn, form the structural units of cells and control all chemical processes within the cell. Think of proteins as the building blocks for an organism, proteins make up your skin, your hair, parts of individual cells. How you look is large ...
Forensic Science Chapter 13
... ____ 13. 2.4 (ch 13) Information from the Human Genome Project will a. reveal the location of a gene on a particular chromosome. b. be useful for diagnosing and treating genetic diseases. c. help to reveal the role and implications of evolution. d. all of the above. ____ 14. 2.5 (ch 13) Restriction ...
... ____ 13. 2.4 (ch 13) Information from the Human Genome Project will a. reveal the location of a gene on a particular chromosome. b. be useful for diagnosing and treating genetic diseases. c. help to reveal the role and implications of evolution. d. all of the above. ____ 14. 2.5 (ch 13) Restriction ...
DNA Lab Techniques
... • Only 2% of human genome codes for proteins (exons) • Other 98% (introns) are non-coding • Only about 20,000 to 25,000 genes (expected 100,000) • Proteome – organism’s complete set of proteins • About 8 million single nucleotide ...
... • Only 2% of human genome codes for proteins (exons) • Other 98% (introns) are non-coding • Only about 20,000 to 25,000 genes (expected 100,000) • Proteome – organism’s complete set of proteins • About 8 million single nucleotide ...
File
... Outline the steps involved in sequencing the genome of an organism. Outline how gene sequencing allows for genome-wide comparisons between individuals and species. Outline how DNA fragments can be separated by size using electrophoresis. Describe how DNA probes can be used to identify fragme ...
... Outline the steps involved in sequencing the genome of an organism. Outline how gene sequencing allows for genome-wide comparisons between individuals and species. Outline how DNA fragments can be separated by size using electrophoresis. Describe how DNA probes can be used to identify fragme ...
Human Genome Project
... – Probe to find fragments containing marker DNA – Sequence 3’ ends – Probe for these sequences, repeat above – Use overlaps in digests to identify fragment order – Gradually move towards gene (Fig. 8.3 P157) ...
... – Probe to find fragments containing marker DNA – Sequence 3’ ends – Probe for these sequences, repeat above – Use overlaps in digests to identify fragment order – Gradually move towards gene (Fig. 8.3 P157) ...
Ch. 11
... 1. _____________________– set of 3 nitrogen bases that represents an amino acid E. Translation: From mRNA to Protein – translation takes place in the ribosome. Transfer RNA (tRNA) bring amino acids to the ribosomal RNA for protein synthesis. 1. ____________________________ – process of converting in ...
... 1. _____________________– set of 3 nitrogen bases that represents an amino acid E. Translation: From mRNA to Protein – translation takes place in the ribosome. Transfer RNA (tRNA) bring amino acids to the ribosomal RNA for protein synthesis. 1. ____________________________ – process of converting in ...
T4 DNA Polymerase
... Recombinant E. coli. Enzyme Storage Buffer 100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol. Enzyme Unit Definition One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):po ...
... Recombinant E. coli. Enzyme Storage Buffer 100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol. Enzyme Unit Definition One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):po ...
Evaluation of a Novel Simple/Complex STR Multiplex for DNA
... A novel marker system for DNA fingerprinting has been developed in Procrea's laboratories. This system presently includes seven STR markers based on Alu-tail polymorphism located on six different chromosomes. In 4 markers, the polymorphic regions consist of simple repeats. The other 3 are made of hi ...
... A novel marker system for DNA fingerprinting has been developed in Procrea's laboratories. This system presently includes seven STR markers based on Alu-tail polymorphism located on six different chromosomes. In 4 markers, the polymorphic regions consist of simple repeats. The other 3 are made of hi ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.