Accuracy of protein flexibility predictions
... use a sliding window averaging technique; parameters are summed for a stretch of amino acids within a window which is shifted by one residue at a time. In the KS method residues have coefficients dependent on the location within the window, thus the contribution of a residue to the prediction value ...
... use a sliding window averaging technique; parameters are summed for a stretch of amino acids within a window which is shifted by one residue at a time. In the KS method residues have coefficients dependent on the location within the window, thus the contribution of a residue to the prediction value ...
A Bayesian network model for protein fold and remote homologue
... after this type of analysis. Recent benchmarking experiments (Brenner et al., 1998; Park et al., 1998; Mueller et al., 1999) using the Structural Classification of Proteins (SCOP) classification of protein structural domains (Murzin et al., 1995) have shown that techniques based on multiple sequence ...
... after this type of analysis. Recent benchmarking experiments (Brenner et al., 1998; Park et al., 1998; Mueller et al., 1999) using the Structural Classification of Proteins (SCOP) classification of protein structural domains (Murzin et al., 1995) have shown that techniques based on multiple sequence ...
Goat Milk - Mt. Capra
... Protein is the substance that makes up virtually every aspect of the human body. Many organs, such as the brain, heart, lungs, muscle, liver, and skin, are made almost entirely of protein. In fact, there are around 200,000 different protein sequences throughout the body. Need64 ...
... Protein is the substance that makes up virtually every aspect of the human body. Many organs, such as the brain, heart, lungs, muscle, liver, and skin, are made almost entirely of protein. In fact, there are around 200,000 different protein sequences throughout the body. Need64 ...
full lab details and projects
... ciliopathies, which often manifest with retinal degeneration. Cilia formation and function depend on the proper placement and anchoring of the basal body at the apical surface, but the mechanisms controlling basal body location remain poorly understood – particularly in photoreceptors. Furthermore, ...
... ciliopathies, which often manifest with retinal degeneration. Cilia formation and function depend on the proper placement and anchoring of the basal body at the apical surface, but the mechanisms controlling basal body location remain poorly understood – particularly in photoreceptors. Furthermore, ...
Publication: Sequence Analysis of Holins by Reduced Amino Acid
... encoded in the genome of bacteriophages to mainly control the phage infection cycle. These genes play two important roles; one is to release the endolysin and other is to determine the timing of the end of infection cycle [2,3]. More than hundred families of holin functional genes have been characte ...
... encoded in the genome of bacteriophages to mainly control the phage infection cycle. These genes play two important roles; one is to release the endolysin and other is to determine the timing of the end of infection cycle [2,3]. More than hundred families of holin functional genes have been characte ...
Characterisation and functional properties of watermelon (Citrullus
... solubility in the pH range 3–5 (data not shown). Protein solubility increased markedly below pH 3 and above pH 5. The considerable increase in protein solubility in acidic and alkaline environments was attributed to a gain in net negative or positive charge of proteins, with consequent interaction w ...
... solubility in the pH range 3–5 (data not shown). Protein solubility increased markedly below pH 3 and above pH 5. The considerable increase in protein solubility in acidic and alkaline environments was attributed to a gain in net negative or positive charge of proteins, with consequent interaction w ...
molecular dynamics studies on mammalian apometallothioneins
... and discussed henceforth. In Figure 2, the rootmean-square deviation (RMSD) of the peptide backbone atoms, with respect to the initial structure are given as a function of time for all apo-MTs. Apo-MT isoforms after 2 ns simulation in vacuum and water The structures obtained at the end of simulation ...
... and discussed henceforth. In Figure 2, the rootmean-square deviation (RMSD) of the peptide backbone atoms, with respect to the initial structure are given as a function of time for all apo-MTs. Apo-MT isoforms after 2 ns simulation in vacuum and water The structures obtained at the end of simulation ...
the versatile bacterial type iv secretion systems
... Although it is clear that the CP coordinates with the Mpf complex to drive DNA transfer, until recently it was not known whether the CP physically interacts with the Mpf structure. Now, two studies have reported that CPs form stable interactions with homologues of the A. tumefaciens VirB10 protein35 ...
... Although it is clear that the CP coordinates with the Mpf complex to drive DNA transfer, until recently it was not known whether the CP physically interacts with the Mpf structure. Now, two studies have reported that CPs form stable interactions with homologues of the A. tumefaciens VirB10 protein35 ...
Murine model of obesity-induced type II diabetes by
... transgenic mice compared with the wild-type controls). They found that the liver glycogen level increased two-fold, whereas the glycogen level was not altered in the skeletal muscle and the brain in the transgenic mice. Further PPP1R3G overexpression reduces hepatic triglyceride in the fed state (15 ...
... transgenic mice compared with the wild-type controls). They found that the liver glycogen level increased two-fold, whereas the glycogen level was not altered in the skeletal muscle and the brain in the transgenic mice. Further PPP1R3G overexpression reduces hepatic triglyceride in the fed state (15 ...
Targeting to the T. gondii plastid
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
Vacuolar protein sorting mechanisms in plants
... RKXRK) and di-aromatic motifs in the cytosolic tail of transmembrane proteins were reported to be important for the export of proteins from the ER in yeast and animals [68]. The first evidence for a di-acidic motif (DAE) in protein export from the ER in plants was obtained for a sugar transporter in ...
... RKXRK) and di-aromatic motifs in the cytosolic tail of transmembrane proteins were reported to be important for the export of proteins from the ER in yeast and animals [68]. The first evidence for a di-acidic motif (DAE) in protein export from the ER in plants was obtained for a sugar transporter in ...
TIBS review article by Killian & Heijne
... (a) Amino acid sequences of WALP and KALP peptides. The N termini are acetylated and the C termini are blocked with either ethanolamine or amide. The flanking residues W and K are highlighted in red and green, respectively. (b) Model of the effect of decreasing the relative length of WALP and KALP p ...
... (a) Amino acid sequences of WALP and KALP peptides. The N termini are acetylated and the C termini are blocked with either ethanolamine or amide. The flanking residues W and K are highlighted in red and green, respectively. (b) Model of the effect of decreasing the relative length of WALP and KALP p ...
Pleiotropy of leptin receptor signalling is defined by distinct roles of
... actions of leptin have also been described [3]. Two effects have been particularly well studied: the stimulation of proinflammatory immune responses by direct action on T-lymphocytes [4,5], and the inhibition of insulin secretion from pancreatic beta cells [6–9]. As a class I cytokine receptor, LEPRb ...
... actions of leptin have also been described [3]. Two effects have been particularly well studied: the stimulation of proinflammatory immune responses by direct action on T-lymphocytes [4,5], and the inhibition of insulin secretion from pancreatic beta cells [6–9]. As a class I cytokine receptor, LEPRb ...
Full text PDF
... thus resulting in the phosphorylation of serine 171 of CRTC2 and its tight association with 14-3-3 proteins in the cytosol. AMP activated protein kinase (AMPK) and its related kinases (AMPKRKs), including salt inducible kinase (SIK)1 and SIK2, are associated with the phosphorylation of this residue ...
... thus resulting in the phosphorylation of serine 171 of CRTC2 and its tight association with 14-3-3 proteins in the cytosol. AMP activated protein kinase (AMPK) and its related kinases (AMPKRKs), including salt inducible kinase (SIK)1 and SIK2, are associated with the phosphorylation of this residue ...
ROR2 Antibody (NT)
... DESCRIPTION: ROR2 (receptor tyrosine kinase-like orphan receptor 2), also known as neurotrophic tyrosine kinase receptor-related 2 (NTRKR2), is a single pass transmembrane tyrosine-protein kinase receptor. It contains a cytoplasmic tyrosine kinase domain, distally located serine-threonine-rich domai ...
... DESCRIPTION: ROR2 (receptor tyrosine kinase-like orphan receptor 2), also known as neurotrophic tyrosine kinase receptor-related 2 (NTRKR2), is a single pass transmembrane tyrosine-protein kinase receptor. It contains a cytoplasmic tyrosine kinase domain, distally located serine-threonine-rich domai ...
Chapter 17 Lecture PowerPoint - McGraw Hill Higher Education
... mRNA secondary structure to control translation initiation • Riboswitches can be used to control translation initiation via mRNA 2° structure – 5’-untranslated region of E. coli thiM mRNA contain a riboswitch – This includes an aptamer that binds thiamine and its metabolite ...
... mRNA secondary structure to control translation initiation • Riboswitches can be used to control translation initiation via mRNA 2° structure – 5’-untranslated region of E. coli thiM mRNA contain a riboswitch – This includes an aptamer that binds thiamine and its metabolite ...
The Endoplasmic Reticulum Glucosyltransferase
... are N-glycosylated (4). The Glc3Man9GlcNAc2 glycan transferred is deglucosylated immediately by the sequential action of glucosidases I and II. At this stage the proteins that are not correctly folded are reglucosylated by UDP-Glc:glycoprotein glucosyltransferase (GT), generating Glc1Man9GlcNAc2. Th ...
... are N-glycosylated (4). The Glc3Man9GlcNAc2 glycan transferred is deglucosylated immediately by the sequential action of glucosidases I and II. At this stage the proteins that are not correctly folded are reglucosylated by UDP-Glc:glycoprotein glucosyltransferase (GT), generating Glc1Man9GlcNAc2. Th ...
Full-Text PDF
... cargo-specific receptor interactions resulting in a signaling cascade that triggers cytoskeletal rearrangements. This causes formation of cell surface extensions that specifically zipper up around the cargo and form a cargo-sized vacuole called the phagosome [66]. Dynamin-2 has been reported to be r ...
... cargo-specific receptor interactions resulting in a signaling cascade that triggers cytoskeletal rearrangements. This causes formation of cell surface extensions that specifically zipper up around the cargo and form a cargo-sized vacuole called the phagosome [66]. Dynamin-2 has been reported to be r ...
Caprotein by Mt. Capra Premium Goat
... This provides many advantages over vegetarian sources (such as soy) because they are typically low in one or more of the amino acids even though overall protein content is high. Why are whole proteins superior to isolated proteins? Protein supplements are often offered as whey protein isolates becau ...
... This provides many advantages over vegetarian sources (such as soy) because they are typically low in one or more of the amino acids even though overall protein content is high. Why are whole proteins superior to isolated proteins? Protein supplements are often offered as whey protein isolates becau ...
HiTrap Chelating HP 1 ml and 5 ml
... coupled to the Sepharose High Performance matrix by stable ether bonds via a seven-atom spacer arm. This gives a very stable adsorbent that can be used over the pH range 4–12. When charged with a suitable metal ion, HiTrap Chelating HP will selectively retain proteins if complex-forming amino acid r ...
... coupled to the Sepharose High Performance matrix by stable ether bonds via a seven-atom spacer arm. This gives a very stable adsorbent that can be used over the pH range 4–12. When charged with a suitable metal ion, HiTrap Chelating HP will selectively retain proteins if complex-forming amino acid r ...
Candidate Genes Predicting Health Vulnerabilities In Families
... 1. Samples may be made up of distinct genetic groups or they have been genetic mixing of groups in recent past (i.e., racial admixture). 2. Groups may differ in allelic distributions and outcomes 3. Creating spurious associations between alleles and outcomes 4. Classic Study: Knowler et al. General ...
... 1. Samples may be made up of distinct genetic groups or they have been genetic mixing of groups in recent past (i.e., racial admixture). 2. Groups may differ in allelic distributions and outcomes 3. Creating spurious associations between alleles and outcomes 4. Classic Study: Knowler et al. General ...
How Translocons Select Transmembrane Helices
... translating the mRNA of a protein targeted for secretion across or insertion into membranes and a signal of a recognition particle (SRP), which is a GTPase. The structures of ribosomes are reviewed in References 18 and 62, and the structure of SRP is reviewed in Reference 54. (Step 2) The ribosome a ...
... translating the mRNA of a protein targeted for secretion across or insertion into membranes and a signal of a recognition particle (SRP), which is a GTPase. The structures of ribosomes are reviewed in References 18 and 62, and the structure of SRP is reviewed in Reference 54. (Step 2) The ribosome a ...
Systematic Analysis of Arabidopsis Organelles
... tagged full-length gene ready for Gateway recombination cloning (Invitrogen). To determine the predictive power of protein localization as an indicator of its function, we analyzed the functional annotations of the collection of Arabidopsis proteins that have been experimentally localized to differe ...
... tagged full-length gene ready for Gateway recombination cloning (Invitrogen). To determine the predictive power of protein localization as an indicator of its function, we analyzed the functional annotations of the collection of Arabidopsis proteins that have been experimentally localized to differe ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).