poster/abstract PDF
... Nicotinic acetylcholine receptors (nAChRs) are involved in signaling throughout the CNS and PNS. Of the many subtypes of nAChRs, α7 containing nAChRs are particularly interesting because of their importance in diseases like Alzheimers and schizophrenia, their importance in modulating other cell sign ...
... Nicotinic acetylcholine receptors (nAChRs) are involved in signaling throughout the CNS and PNS. Of the many subtypes of nAChRs, α7 containing nAChRs are particularly interesting because of their importance in diseases like Alzheimers and schizophrenia, their importance in modulating other cell sign ...
Scavenging of 14-3-3 proteins reveals their involvement in the cell
... oocytes expressing pGpLI-R18 were incubated with IgGSepharose (Fig. 1B). This resulted in a cellular extract depleted of 14-3-3 proteins, and an eluate containing both pGpLI-R18 and 14-3-3 proteins. This indicates that a large portion of cellular 14-3-3 proteins are bound to the 14-3-3 scavenger: co ...
... oocytes expressing pGpLI-R18 were incubated with IgGSepharose (Fig. 1B). This resulted in a cellular extract depleted of 14-3-3 proteins, and an eluate containing both pGpLI-R18 and 14-3-3 proteins. This indicates that a large portion of cellular 14-3-3 proteins are bound to the 14-3-3 scavenger: co ...
Lecture 4 Enzymes Catalytic proteins Enzymes Enzymes Enzymes
... of the active site into new positions enhancing the ability of the enzyme to catalyse the reaction • The rest of the protein structure provides the structural framework that determines the configuration of the active site ...
... of the active site into new positions enhancing the ability of the enzyme to catalyse the reaction • The rest of the protein structure provides the structural framework that determines the configuration of the active site ...
Muscle
... on actin filaments, which subsequently leads to the troponin complex being physically moved aside to uncover cross-bridge binding sites on the actin filament. ...
... on actin filaments, which subsequently leads to the troponin complex being physically moved aside to uncover cross-bridge binding sites on the actin filament. ...
Slides
... Probabilistic Ensembles for Improved Inference in Protein-Structure Determination Ameet Soni* and Jude Shavlik Dept. of Computer Sciences Dept. of Biostatistics and Medical Informatics ...
... Probabilistic Ensembles for Improved Inference in Protein-Structure Determination Ameet Soni* and Jude Shavlik Dept. of Computer Sciences Dept. of Biostatistics and Medical Informatics ...
Site directed mutagenesis as an efficient way to enhance structural
... Green fluorescence protein (GFP) was first found and isolated from a jelly fish Aequoreavictorea (Tsien, 1998).There are many coelenterates that have this protein but those that are well studied and characterised are from Aequorea and Renilla. But so far scientists were able to clone only GFP from A ...
... Green fluorescence protein (GFP) was first found and isolated from a jelly fish Aequoreavictorea (Tsien, 1998).There are many coelenterates that have this protein but those that are well studied and characterised are from Aequorea and Renilla. But so far scientists were able to clone only GFP from A ...
chemistry bulletin 2005
... E. coli era was isolated as a suppressor of a temperature sensitive dnaG mutant encoding DNA primase (Britton et al. 1997). Microarray and other studies have linked era function with energy metabolism (Inoue et al. 2002; Pillutla et al. 1996; Powell et al. 1995). Much phenotypic work on era has sugg ...
... E. coli era was isolated as a suppressor of a temperature sensitive dnaG mutant encoding DNA primase (Britton et al. 1997). Microarray and other studies have linked era function with energy metabolism (Inoue et al. 2002; Pillutla et al. 1996; Powell et al. 1995). Much phenotypic work on era has sugg ...
PDF - World Wide Journals
... obtained from polysaccharides, lipid and proteins. Proteins are polymers formed by various amino acids capable of promoting intra- and inter-molecular bonds, allowing the resultant materials to have a large variation in their functional properties (Arun ...
... obtained from polysaccharides, lipid and proteins. Proteins are polymers formed by various amino acids capable of promoting intra- and inter-molecular bonds, allowing the resultant materials to have a large variation in their functional properties (Arun ...
Homology Modeling a Fast Tool for Drug Discovery
... the tough requirement of force field and enormous conformation searching, because it deals with the calculation of a force field and replaces it in large part, with the counting of sequence identities [7] . The method is based on the fact that structural conformation of a protein is more highly cons ...
... the tough requirement of force field and enormous conformation searching, because it deals with the calculation of a force field and replaces it in large part, with the counting of sequence identities [7] . The method is based on the fact that structural conformation of a protein is more highly cons ...
A Series of Ubiquitin Binding Factors Connects CDC48/p97 to
... Proteolysis is pivotal for cellular and developmental regulation. Due to its irreversible nature, proteolysis is ideally suited for regulating unidirectional pathways such as cell cycle progression or differentiation. In eukaryotes, selective proteolysis is largely mediated by the ubiquitin/ proteas ...
... Proteolysis is pivotal for cellular and developmental regulation. Due to its irreversible nature, proteolysis is ideally suited for regulating unidirectional pathways such as cell cycle progression or differentiation. In eukaryotes, selective proteolysis is largely mediated by the ubiquitin/ proteas ...
A Novel Role for Vitamin K1 in a Tyrosine Phosphorylation
... type III and immunoglobulin motifs common to extracellular matrix proteins, neural cell adhesion molecules, and cell surface receptors with tyrosine kinase or phosphatase activities (13, 14). It is believed that these RTKs may be bifunctional, acting both as cell adhesion proteins and as components ...
... type III and immunoglobulin motifs common to extracellular matrix proteins, neural cell adhesion molecules, and cell surface receptors with tyrosine kinase or phosphatase activities (13, 14). It is believed that these RTKs may be bifunctional, acting both as cell adhesion proteins and as components ...
Sequential steps in clathrin-mediated synaptic vesicle endocytosis
... clathrin assembly and which appears to be critical for the generation of synaptic vesicles with an homogenous size. In Drosophila and C. elegans lacking AP180-like proteins, nerve terminals still contain synaptic vesicles, but their average size is larger and the size variability is increased when c ...
... clathrin assembly and which appears to be critical for the generation of synaptic vesicles with an homogenous size. In Drosophila and C. elegans lacking AP180-like proteins, nerve terminals still contain synaptic vesicles, but their average size is larger and the size variability is increased when c ...
Neurotrophins: the biological paradox of survival factors
... After binding to their respective ligands, the Fas and TNF receptors initiate an irreversible set of events culminating in apoptosis. A ligand-dependent mechanism has also been observed with the p75 receptor (Casaccia-Bonnefil et al, 1996b; Frade et al, 1996). Administration of antibodies against NG ...
... After binding to their respective ligands, the Fas and TNF receptors initiate an irreversible set of events culminating in apoptosis. A ligand-dependent mechanism has also been observed with the p75 receptor (Casaccia-Bonnefil et al, 1996b; Frade et al, 1996). Administration of antibodies against NG ...
Lysosomes and lysosomal disorders
... In many lysosomal disorders are stored also metabolites unrelated to the primary defect, very often lipids or hydrophobic proteins Frequently gangliosides GM3, GM2 or cholesterol ... although the protein machinery for their degradation or transport is intact Example: in some mucopolysaccharidoses (s ...
... In many lysosomal disorders are stored also metabolites unrelated to the primary defect, very often lipids or hydrophobic proteins Frequently gangliosides GM3, GM2 or cholesterol ... although the protein machinery for their degradation or transport is intact Example: in some mucopolysaccharidoses (s ...
SpeeDB: fast structural protein searches
... cation–p interactions. Burley and Petsko (1985) analyzed a total of 34 crystal structures of proteins for aromatic–aromatic interactions and identified that on average, 61% of phenylalanine, 54% of tyrosine and 59% of tryptophan residues are involved in aromatic– aromatic interactions. They also fou ...
... cation–p interactions. Burley and Petsko (1985) analyzed a total of 34 crystal structures of proteins for aromatic–aromatic interactions and identified that on average, 61% of phenylalanine, 54% of tyrosine and 59% of tryptophan residues are involved in aromatic– aromatic interactions. They also fou ...
HHMI meeting, FOLDING
... Found: metastable (“accumulating”, “directly observable”) folding intermediates. The idea was: intermediates will help to trace the folding pathway, - like intermediates in a biochemical reaction trace its pathway. This was a “chemical logic”. However, although protein folding intermediates (like M ...
... Found: metastable (“accumulating”, “directly observable”) folding intermediates. The idea was: intermediates will help to trace the folding pathway, - like intermediates in a biochemical reaction trace its pathway. This was a “chemical logic”. However, although protein folding intermediates (like M ...
Full-Text PDF
... A main issue is that putative vacuolar sorting receptor(s) for CtVSD proteins have been identified, but their sorting mechanism remains unclear. The CtVSD are not specifically recognized by the well-characterized Vacuolar Sorting Receptors (VSRs), which have a strong affinity for ssVSD [47,48]. Thes ...
... A main issue is that putative vacuolar sorting receptor(s) for CtVSD proteins have been identified, but their sorting mechanism remains unclear. The CtVSD are not specifically recognized by the well-characterized Vacuolar Sorting Receptors (VSRs), which have a strong affinity for ssVSD [47,48]. Thes ...
Translation of an integral membrane protein in distal dendrites of
... 2000), and disruption of these elements impairs persistent plasticity and memory consolidation (Miller et al., 2002). Dendritic localization of specific mRNAs can be stimulated by synaptic activity or depolarization (Muslimov et al., 1998; Steward et al., 1998; Zhang et al., 1999; Mori et al., 2000; ...
... 2000), and disruption of these elements impairs persistent plasticity and memory consolidation (Miller et al., 2002). Dendritic localization of specific mRNAs can be stimulated by synaptic activity or depolarization (Muslimov et al., 1998; Steward et al., 1998; Zhang et al., 1999; Mori et al., 2000; ...
Retinal Neurotransmitters
... mitochondrial matrix to drive 2-oxoglutarate synthesis or escape to the cytosol via porin channels. Glutamate Transporters. Vesicular glutamate import is mediated by VGluT1 and VGluT2 transporters, members of the Na+-dependent plasma membrane PO4- symporter family (e.g. Takamori et al., 2000). VGluT ...
... mitochondrial matrix to drive 2-oxoglutarate synthesis or escape to the cytosol via porin channels. Glutamate Transporters. Vesicular glutamate import is mediated by VGluT1 and VGluT2 transporters, members of the Na+-dependent plasma membrane PO4- symporter family (e.g. Takamori et al., 2000). VGluT ...
a study of intelligent techniques for protein secondary structure
... Qian and Terrence [Qian et al, 1988] introduced one of the first Neural Networks used for secondary structure prediction. They worked on a network with 17 input groups having 21 units per group, 40 hidden units and three output units. The usage of Neural Networks then started evolving and different ...
... Qian and Terrence [Qian et al, 1988] introduced one of the first Neural Networks used for secondary structure prediction. They worked on a network with 17 input groups having 21 units per group, 40 hidden units and three output units. The usage of Neural Networks then started evolving and different ...
Novel plasmodesmata association of dehydrin
... by Close et al. 1993a, 1993b, Close 1996, 1997, Campbell and Close 1997). Members of this protein family are characterized by the presence of a lysine-rich consensus sequence (EKKGI MDKIKEKLPG), a tract of serine residues and typically 1–3 Y motifs (DEYGNP) near the N-terminus (Close et al. 1993b, C ...
... by Close et al. 1993a, 1993b, Close 1996, 1997, Campbell and Close 1997). Members of this protein family are characterized by the presence of a lysine-rich consensus sequence (EKKGI MDKIKEKLPG), a tract of serine residues and typically 1–3 Y motifs (DEYGNP) near the N-terminus (Close et al. 1993b, C ...
Chapters 9 and 10 Lipids and Membranes Lipids
... These are channels in the membrane that permit the rapid movement of specific molecules across the membrane. The tertiary and quaternary structure of integral membrane proteins create an aqueous hole in the membrane. The movement of solute through a channel is always from higher to lower concentrati ...
... These are channels in the membrane that permit the rapid movement of specific molecules across the membrane. The tertiary and quaternary structure of integral membrane proteins create an aqueous hole in the membrane. The movement of solute through a channel is always from higher to lower concentrati ...
Repeat proteins challenge the concept of structural domains
... correlate with biological activities and many modern proteins can be described as composed by novel ‘domain arrangements’ [2]. For globular proteins, this fact facilitates the description, evolution and construction of single amino acid chains that comprise a set of integrated biological functions, ...
... correlate with biological activities and many modern proteins can be described as composed by novel ‘domain arrangements’ [2]. For globular proteins, this fact facilitates the description, evolution and construction of single amino acid chains that comprise a set of integrated biological functions, ...
Proteomics of spermatogenesis: from protein lists to understanding
... testis at specific time points (days 0, 7, 14, 21, 28 and 60 postpartum) and identified 362 differential protein spots corresponding to 257 different proteins involved in the initiation of mouse spermatogenesis. Furthermore, cluster analysis revealed six expression patterns, and bioinformatics analy ...
... testis at specific time points (days 0, 7, 14, 21, 28 and 60 postpartum) and identified 362 differential protein spots corresponding to 257 different proteins involved in the initiation of mouse spermatogenesis. Furthermore, cluster analysis revealed six expression patterns, and bioinformatics analy ...
Full-Text
... disordered. Amyloids are highly ordered β-sheet rich protein assemblies that have been found in a variety of functional or pathogenic contexts. Mammalian prions are characterized by their ability to be infectious and self-replicating [35]. In humans they are associated with neurodegenerative disease ...
... disordered. Amyloids are highly ordered β-sheet rich protein assemblies that have been found in a variety of functional or pathogenic contexts. Mammalian prions are characterized by their ability to be infectious and self-replicating [35]. In humans they are associated with neurodegenerative disease ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).