The Cell Adhesion Molecule Neuroplastin
... Na2HPO4, pH 7.3). Cells were then detached from the culture dishes by incubating with 2.5 ml of 5 mM EDTA in PBS for 5 min at room temperature. The resulting cell suspension was diluted in 6 ml of cold Dulbecco’s modified Eagle’s medium and centrifuged for 5 min at 1,500 rpm. The cell pellet from fo ...
... Na2HPO4, pH 7.3). Cells were then detached from the culture dishes by incubating with 2.5 ml of 5 mM EDTA in PBS for 5 min at room temperature. The resulting cell suspension was diluted in 6 ml of cold Dulbecco’s modified Eagle’s medium and centrifuged for 5 min at 1,500 rpm. The cell pellet from fo ...
Biological Science, 5e (Freeman) Chapter 3 Protein Structure and
... create a pattern that can be analyzed mathematically to determine the three-dimensional structure of the protein. This analysis has been performed by Palczewski (2000) on the protein rhodopsin, which is a light-sensitive protein found in species ranging from ancient bacteria (archaea) to humans. The ...
... create a pattern that can be analyzed mathematically to determine the three-dimensional structure of the protein. This analysis has been performed by Palczewski (2000) on the protein rhodopsin, which is a light-sensitive protein found in species ranging from ancient bacteria (archaea) to humans. The ...
A Survey of Left-handed Helices in Protein Structures
... available in which no support could be found for any left-handed helical conformation, and in one case there was no support in the electron density (although the structure had been determined at 1.9 Å resolution and refined to a crystallographic R-value of 0.2). Finally, one entry was accepted in g ...
... available in which no support could be found for any left-handed helical conformation, and in one case there was no support in the electron density (although the structure had been determined at 1.9 Å resolution and refined to a crystallographic R-value of 0.2). Finally, one entry was accepted in g ...
Molecular architecture of the pyruvate dehydrogenase complex
... E3 respectively. However, in Gram-positive bacteria such as G. stearothermophilus, E1 and E3 both associate with E2, as E3BP features only in eukaryotes. Consequently, E1 and E3 have to compete for overlapping binding sites on E2; interaction of E2 with either E1 or E3 prevents complex formation wit ...
... E3 respectively. However, in Gram-positive bacteria such as G. stearothermophilus, E1 and E3 both associate with E2, as E3BP features only in eukaryotes. Consequently, E1 and E3 have to compete for overlapping binding sites on E2; interaction of E2 with either E1 or E3 prevents complex formation wit ...
Homology modeling workshop
... • Restraints were obtained also from a statistical analysis of the relationships from a large database of pairs of homologous structures. • Various correlations were obtained, e.g. correlations between CaCa distances. These relationships can be used directly as spatial restraints. • Restraints and C ...
... • Restraints were obtained also from a statistical analysis of the relationships from a large database of pairs of homologous structures. • Various correlations were obtained, e.g. correlations between CaCa distances. These relationships can be used directly as spatial restraints. • Restraints and C ...
![Basic region of residues 228-231 of protein kinase CK1[alpha] is](http://s1.studyres.com/store/data/015745345_1-81da768c37e720623b4b1898472e2f2e-300x300.png)
Basic region of residues 228-231 of protein kinase CK1[alpha] is
... e) some of which have specific physiological functions [Rowles et al., 1991; Tapia et al., 1994; Fish et al., 1995]. Some isoforms, such as CK1a, have several splicing variants that have different biochemical and cellular properties [Burzio et al., 2002]. In this article, results are presented which ...
... e) some of which have specific physiological functions [Rowles et al., 1991; Tapia et al., 1994; Fish et al., 1995]. Some isoforms, such as CK1a, have several splicing variants that have different biochemical and cellular properties [Burzio et al., 2002]. In this article, results are presented which ...
Hitting the Sweet Spot-Glycans as Targets of Fungal Defense
... structures of several plant representatives of this protein family, the polypeptide is folded into three domains, the largest forming a lectin-like β-barrel. The thaumatin fold is stabilized, similar to defensins and some fungal LysM-proteins, by eight disulfide bridges. Despite the structural infor ...
... structures of several plant representatives of this protein family, the polypeptide is folded into three domains, the largest forming a lectin-like β-barrel. The thaumatin fold is stabilized, similar to defensins and some fungal LysM-proteins, by eight disulfide bridges. Despite the structural infor ...
Infectious Salmon Anaemia Virus (ISAV) RNA Binding Protein
... IBV has also occasionally been detected in seals [17–19]. For ISAV, previous studies have indicated that a low-virulent strain induced the antiviral proteins Mx and ISG15 more potently than a high virulent ISAV strain early in the infection cycle in cell cultures [20]. Similarly, infection with a lo ...
... IBV has also occasionally been detected in seals [17–19]. For ISAV, previous studies have indicated that a low-virulent strain induced the antiviral proteins Mx and ISG15 more potently than a high virulent ISAV strain early in the infection cycle in cell cultures [20]. Similarly, infection with a lo ...
1 Biochemistry 462a – Hemoglobin Structure and Function Reading
... 3. The presence of BPG aids the delivery of O2 by favoring the deoxy conformation. 4. Deoxy Hb binds CO2. 5. The deoxy Hb returns to the lungs where the pH is higher, the O2 content higher and the CO2 content is lower. All these factors favor reverse of carbamate formation (loss of bound CO2), depro ...
... 3. The presence of BPG aids the delivery of O2 by favoring the deoxy conformation. 4. Deoxy Hb binds CO2. 5. The deoxy Hb returns to the lungs where the pH is higher, the O2 content higher and the CO2 content is lower. All these factors favor reverse of carbamate formation (loss of bound CO2), depro ...
Localization of TGN38 to the trans-Golgi Network
... that whereas normal Tac was expressed on the plasma membrane (Fig. 1 a), the T-G-G chimera was localized to a juxtanuclear, tubulo-vesicular structure in the majority of positively stained cells (Fig. 1 c). This distribution was consistent with localization to the TGN, as demonstrated by the similar ...
... that whereas normal Tac was expressed on the plasma membrane (Fig. 1 a), the T-G-G chimera was localized to a juxtanuclear, tubulo-vesicular structure in the majority of positively stained cells (Fig. 1 c). This distribution was consistent with localization to the TGN, as demonstrated by the similar ...
bioinformatics - Noble Research Lab
... 1 INTRODUCTION Most proteins perform their functions by interacting with other proteins.Therefore, information about the network of interactions that occur in a cell can greatly increase our understanding of protein function. Several experimental assays that probe interactions in a high-throughput m ...
... 1 INTRODUCTION Most proteins perform their functions by interacting with other proteins.Therefore, information about the network of interactions that occur in a cell can greatly increase our understanding of protein function. Several experimental assays that probe interactions in a high-throughput m ...
Dietary Guidelines should reflect new understandings about adult
... During the past decade a growing body of research reveals that dietary protein intakes above the RDA are beneficial in maintaining muscle function and mobility [6] and in the treatment of diseases including obesity, osteoporosis, type 2 diabetes (T2DM), Metabolic Syndrome (MetS), heart disease, and ...
... During the past decade a growing body of research reveals that dietary protein intakes above the RDA are beneficial in maintaining muscle function and mobility [6] and in the treatment of diseases including obesity, osteoporosis, type 2 diabetes (T2DM), Metabolic Syndrome (MetS), heart disease, and ...
Fast and simple purification of GST fusion proteins using prepacked
... capacity will vary depending on flow rate and sample properties. Glutathione Sepharose 4 Fast Flow is available in lab packs for scale-up. Table 1 lists the main characteristics of GSTrap and Glutathione Sepharose 4 Fast Flow. ...
... capacity will vary depending on flow rate and sample properties. Glutathione Sepharose 4 Fast Flow is available in lab packs for scale-up. Table 1 lists the main characteristics of GSTrap and Glutathione Sepharose 4 Fast Flow. ...
PLANT PROTEIN PHOSPHATASES
... dephosphorylation of glycogen-bound phosphoenzymes required for glycogen synthesis and accumulation (35). A nuclear-localized PP1 regulatory subunit, sds22+, which is essential for completion of mitosis in S. pombe, increases phosphohistone H1 phosphatase activity of PP1 and downregulates its activi ...
... dephosphorylation of glycogen-bound phosphoenzymes required for glycogen synthesis and accumulation (35). A nuclear-localized PP1 regulatory subunit, sds22+, which is essential for completion of mitosis in S. pombe, increases phosphohistone H1 phosphatase activity of PP1 and downregulates its activi ...
Nutrient Metabolism
... Fat adaptation refers to the combined effect of training and prolonged consumption of a high fat (HF) diet (Kronfeld and Downey 1981). Fat adaptation has influenced acid-base responses to repeated sprints, mainly by reducing the increase in PCO2 in venous blood (Kronfeld et al. 1998). An increase in ...
... Fat adaptation refers to the combined effect of training and prolonged consumption of a high fat (HF) diet (Kronfeld and Downey 1981). Fat adaptation has influenced acid-base responses to repeated sprints, mainly by reducing the increase in PCO2 in venous blood (Kronfeld et al. 1998). An increase in ...
as PDF
... but display highly similar homo-trimeric three-dimensional structures (figure 2) (13). The signaling receptors used by gAcrp30 and adiponectin have recently been cloned, and named AdipoR1 and AdipoR2 (15). Based on their amino-acid sequences, AdipoR1 and AdipoR2 are structurally-related, and are pre ...
... but display highly similar homo-trimeric three-dimensional structures (figure 2) (13). The signaling receptors used by gAcrp30 and adiponectin have recently been cloned, and named AdipoR1 and AdipoR2 (15). Based on their amino-acid sequences, AdipoR1 and AdipoR2 are structurally-related, and are pre ...
311-320 - CiteSeerX
... be perturbation of the aromatic amino acid residues. This is followed by the exposure of the buried aromatic amino acids and finally by a conformational change leading to an increase in hydrodynamic volume. Such an analysis of the observed data is, however, complicated by the dissociation of the oli ...
... be perturbation of the aromatic amino acid residues. This is followed by the exposure of the buried aromatic amino acids and finally by a conformational change leading to an increase in hydrodynamic volume. Such an analysis of the observed data is, however, complicated by the dissociation of the oli ...
Additional file 1
... Important role in pollens and anther development in response to gibberellic acid Transcriptional regulation Transcription factors ...
... Important role in pollens and anther development in response to gibberellic acid Transcriptional regulation Transcription factors ...
Overcoming stalled translation in human mitochondria
... open reading frames, what are the functions of the remaining 3 mitochondrial RF family members? Is there any evidence that they can still function as RFs? These proteins were grouped together due to similarities in their sequence and structures that they share with RFs from bacteria and the eukaryot ...
... open reading frames, what are the functions of the remaining 3 mitochondrial RF family members? Is there any evidence that they can still function as RFs? These proteins were grouped together due to similarities in their sequence and structures that they share with RFs from bacteria and the eukaryot ...
Planta
... production of the phytonutrients that are essential for a balanced diet and for disease prevention. The pathways involved in these processes are unique to plants and vary between species. Climacteric fruit ripening, especially in tomato, has been extensively studied; yet, ripening of nonclimacteric ...
... production of the phytonutrients that are essential for a balanced diet and for disease prevention. The pathways involved in these processes are unique to plants and vary between species. Climacteric fruit ripening, especially in tomato, has been extensively studied; yet, ripening of nonclimacteric ...
Src protein–tyrosine kinase structure and regulation
... The human Src gene encodes a protein of 536 amino acids, and the chicken Src gene encodes a 533-residue protein. The three additional amino acids in humans are inserted near the amino terminus. For historical reasons, the chicken numbering system is used in most of the literature, even when studies ...
... The human Src gene encodes a protein of 536 amino acids, and the chicken Src gene encodes a 533-residue protein. The three additional amino acids in humans are inserted near the amino terminus. For historical reasons, the chicken numbering system is used in most of the literature, even when studies ...
Characterization of Lipid Rafts from Medicago
... evaluate the contamination by other membranes. Measurement of the vanadate-sensitive ATPase activity showed a 7-fold enrichment in PM proteins after phase partition compared with the starting material consisting of a crude microsomal fraction; a classical ratio for this technique. The mitochondrial ...
... evaluate the contamination by other membranes. Measurement of the vanadate-sensitive ATPase activity showed a 7-fold enrichment in PM proteins after phase partition compared with the starting material consisting of a crude microsomal fraction; a classical ratio for this technique. The mitochondrial ...
Ammonium Dodecyl Sulfate as an Alternative to Sodium Dodecyl
... cannot be predicted in the protein MALDI spectrum, the degree of mass shift is unknown. As a consequence, the protein mass cannot be accurately defined. Effects of ADS, TDS, and HDS on Resolution. SDS is commonly used to extract hydrophobic or membrane proteins from cells.5,6 Most biopolymer samples ...
... cannot be predicted in the protein MALDI spectrum, the degree of mass shift is unknown. As a consequence, the protein mass cannot be accurately defined. Effects of ADS, TDS, and HDS on Resolution. SDS is commonly used to extract hydrophobic or membrane proteins from cells.5,6 Most biopolymer samples ...
Identification of a putative flexible loop in Arabidopsis glutathione
... The crystal structures of both GSH synthetase [7] and alanine : -alanine ligase [8] from Escherichia coli have been solved. While these two enzymes show few similarities in their primary sequence, there is substantial similarity in their threedimensional structures. In both enzymes, substrate bind ...
... The crystal structures of both GSH synthetase [7] and alanine : -alanine ligase [8] from Escherichia coli have been solved. While these two enzymes show few similarities in their primary sequence, there is substantial similarity in their threedimensional structures. In both enzymes, substrate bind ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).