Somatic cancer mutations in the MLL1 histone methyltransferase
... Somatic missense mutations in the mixed lineage leukemia 1 (MLL1) histone H3K4 methyltransferase are often observed in cancers. MLL1 forms a complex with WDR5, RBBP5, and ASH2L (WRA) which stimulates its activity. The MM-102 compound prevents the interaction between MLL1 and WDR5 and functions as an ...
... Somatic missense mutations in the mixed lineage leukemia 1 (MLL1) histone H3K4 methyltransferase are often observed in cancers. MLL1 forms a complex with WDR5, RBBP5, and ASH2L (WRA) which stimulates its activity. The MM-102 compound prevents the interaction between MLL1 and WDR5 and functions as an ...
Protein Structure and Interactions
... In mammals small heat-shock proteins (sHsp) comprises 10 members, many of which are considered as molecular chaperones based on demonstrated in vitro activity. Whether these proteins behave as chaperones in vivo remains unclear. Here, we investigated the effect of HspB8 on the expression of Htt43Q, ...
... In mammals small heat-shock proteins (sHsp) comprises 10 members, many of which are considered as molecular chaperones based on demonstrated in vitro activity. Whether these proteins behave as chaperones in vivo remains unclear. Here, we investigated the effect of HspB8 on the expression of Htt43Q, ...
Topology Prediction of Membrane Proteins
... lipids, which separates them from the outside world. The membrane is a physical barrier that protects the cell from foreign molecules at the same time as it prevents leakage of internal components and substances. However, a cell must be able to communicate with its surroundings, exchange molecules a ...
... lipids, which separates them from the outside world. The membrane is a physical barrier that protects the cell from foreign molecules at the same time as it prevents leakage of internal components and substances. However, a cell must be able to communicate with its surroundings, exchange molecules a ...
AHEART December 46/6 - AJP
... The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. H2442 ...
... The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. H2442 ...
structural basis for thermal stability of thermophilic trmd proteins
... unstable and degrade. How the thermophiles are able to thrive in boiling water is based on the protein composition. Research has shown that there are various components of structure that allow for the stability of thermophilic proteins at higher temperatures. ...
... unstable and degrade. How the thermophiles are able to thrive in boiling water is based on the protein composition. Research has shown that there are various components of structure that allow for the stability of thermophilic proteins at higher temperatures. ...
ANALYSIS OF A BACTERIAL SERINE/THREONINE KINASE
... transduction. Expression of at least forty proteins is thought to be under control of the PhoPQ TCS (Véscovi et al., 1994). This is based on two dimensional gel analyses. The PhoPQ system was first recognized for its role in virulence in 1989. Miller et al. discovered that mutations in the phoP locu ...
... transduction. Expression of at least forty proteins is thought to be under control of the PhoPQ TCS (Véscovi et al., 1994). This is based on two dimensional gel analyses. The PhoPQ system was first recognized for its role in virulence in 1989. Miller et al. discovered that mutations in the phoP locu ...
Intracellular Redox Compartmentation and ROS
... Recent years have witnessed enormous progress in understanding redox signaling related to reactive oxygen species (ROS) in plants. The consensus view is that such signaling is intrinsic to many developmental processes and responses to the environment. ROS-related redox signaling is tightly wedded to ...
... Recent years have witnessed enormous progress in understanding redox signaling related to reactive oxygen species (ROS) in plants. The consensus view is that such signaling is intrinsic to many developmental processes and responses to the environment. ROS-related redox signaling is tightly wedded to ...
Sequence-Specific Deoxyribonucleic Acid (DNA) Recognition by
... common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-! gene that encodes a subunit of a reproductive hormone. SF1 forms a specific ...
... common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-! gene that encodes a subunit of a reproductive hormone. SF1 forms a specific ...
Affinity Chromatography
... inhomogeneity problems where there were multiple sites on the target ligand or the coupling chemistries were incomplete, whereas the pre-assembled ligand spacer arm unit could be pre-characterized by conventional chemical techniques and studies in solution to yield useful advance information on bind ...
... inhomogeneity problems where there were multiple sites on the target ligand or the coupling chemistries were incomplete, whereas the pre-assembled ligand spacer arm unit could be pre-characterized by conventional chemical techniques and studies in solution to yield useful advance information on bind ...
Tsui, S, Dai, T, Warren, ST and Yen, P: Association of the mouse infertility factor DAZL1 with actively translating polyribosomes. Biology of Reproduction 62:1655-1660 (2000).
... A requirement of the Y chromosome long arm (Yq) for normal spermatogenesis was recognized more than two decades ago when six infertile men were found to have Yq deletions [1]. More recent molecular screening has detected Yq microdeletions in 10–15% of males with idiopathic azoospermia [2–4]. The DAZ ...
... A requirement of the Y chromosome long arm (Yq) for normal spermatogenesis was recognized more than two decades ago when six infertile men were found to have Yq deletions [1]. More recent molecular screening has detected Yq microdeletions in 10–15% of males with idiopathic azoospermia [2–4]. The DAZ ...
A Survey of Flexible Protein Binding Mechanisms and their
... Such understanding may lead to the ability to design partners that form more stable complexes, which can then act as “network” drugs. Understanding interactions will also help us to find ways of inhibiting pathogenic association processes such as irreversible aggregation. Biomolecular recognition pr ...
... Such understanding may lead to the ability to design partners that form more stable complexes, which can then act as “network” drugs. Understanding interactions will also help us to find ways of inhibiting pathogenic association processes such as irreversible aggregation. Biomolecular recognition pr ...
Mitochondrial protein acetylation regulates metabolism
... mitochondrion. Most lysine-acetylated proteins identified in mitochondrial fractions were metabolic enzymes. Lysine acetylation was also identified on the mitochondrial DNA-encoded ATP synthase Fo subunit 8, implying that proteins can become acetylated within mitochondria. The acetylation status of ...
... mitochondrion. Most lysine-acetylated proteins identified in mitochondrial fractions were metabolic enzymes. Lysine acetylation was also identified on the mitochondrial DNA-encoded ATP synthase Fo subunit 8, implying that proteins can become acetylated within mitochondria. The acetylation status of ...
The Tomato Calcium Sensor Cbl10 and Its Interacting - IBVF
... ROS burst stimulated extracellular Ca2+ influx to the cytosol (Lecourieux et al., 2002). The main enzymatic source for the oxidative bursts in Arabidopsis thaliana, the membrane-bound NADPH oxidases (also known as respiratory burst homolog [RBOH] proteins), are synergistically regulated by Ca2+ and p ...
... ROS burst stimulated extracellular Ca2+ influx to the cytosol (Lecourieux et al., 2002). The main enzymatic source for the oxidative bursts in Arabidopsis thaliana, the membrane-bound NADPH oxidases (also known as respiratory burst homolog [RBOH] proteins), are synergistically regulated by Ca2+ and p ...
The Heat-Shock Proteins
... relative position in the protein. The E. coli protein is missing this segment. All of the proteins, including that of E. coli. contain another, smaller region of high negative-charge density toward the carboxy terminus. The carboxyterminal regions of these proteins are generally the most divergent, ...
... relative position in the protein. The E. coli protein is missing this segment. All of the proteins, including that of E. coli. contain another, smaller region of high negative-charge density toward the carboxy terminus. The carboxyterminal regions of these proteins are generally the most divergent, ...
F-actin Sequesters Elongation Factor from Interaction with
... ton (Howe and Hershey, 1984; Gavrilova et al., 1987; Zambetti et al., 1990; Hamill et al., 1994; Hesketh et al., 1991). Interestingly, the other elongation factor (EF-2) has been demonstrated to bind directly to actin filaments (Bektas et al., 1994). Colocalization of these components with the cytos ...
... ton (Howe and Hershey, 1984; Gavrilova et al., 1987; Zambetti et al., 1990; Hamill et al., 1994; Hesketh et al., 1991). Interestingly, the other elongation factor (EF-2) has been demonstrated to bind directly to actin filaments (Bektas et al., 1994). Colocalization of these components with the cytos ...
A Simple Method for Displaying the Hydropathic Character of a Protein
... individual amino acid side-chains between ethanol and water from these values had already been formulated (Cohn & Edsell, 1943). The transfer free energies from water to ethanol for various amino acid side-chains are presented in Table 1, The assumption that ethanol is a neutral, non-interacting sol ...
... individual amino acid side-chains between ethanol and water from these values had already been formulated (Cohn & Edsell, 1943). The transfer free energies from water to ethanol for various amino acid side-chains are presented in Table 1, The assumption that ethanol is a neutral, non-interacting sol ...
Keep Your Fingers Off My DNA: Protein–Protein
... Gata1 (FOG1) and its binding partners, the globin transcription factor GATA1 and Transforming Acidic Coiledcoil 3 (TACC3). FOG1 contains a total of nine ZFs (Table 2); however, only four are of the classic C2H2 type. The remaining fingers substitute a conserved cysteine for the final histidine [36]. ...
... Gata1 (FOG1) and its binding partners, the globin transcription factor GATA1 and Transforming Acidic Coiledcoil 3 (TACC3). FOG1 contains a total of nine ZFs (Table 2); however, only four are of the classic C2H2 type. The remaining fingers substitute a conserved cysteine for the final histidine [36]. ...
Monte Carlo simulations of peptide–membrane interactions with the
... the user is taken as the initial conformation. For simulations in the aqueous phase (i.e. without the membrane), the initial structure is used as it is. The simulations are carried out in three to five independent runs of 500 000–900 000 MC cycles each; the recommended number and length of the runs a ...
... the user is taken as the initial conformation. For simulations in the aqueous phase (i.e. without the membrane), the initial structure is used as it is. The simulations are carried out in three to five independent runs of 500 000–900 000 MC cycles each; the recommended number and length of the runs a ...
SpyTag and SpyCatcher - Department of Biochemistry
... SpyTag/SpyCatcher has shown a range of intriguing applications, but every nascent technology requires optimisation. In our hands, fusion to SpyTag rarely disturbs function or yield. However, SpyCatcher is a larger fusion, even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is als ...
... SpyTag/SpyCatcher has shown a range of intriguing applications, but every nascent technology requires optimisation. In our hands, fusion to SpyTag rarely disturbs function or yield. However, SpyCatcher is a larger fusion, even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is als ...
Calcium Homeostasis in Plants: Role of Calcium Binding Proteins in
... mechanism of 'transfer' of this information from outer surface to the core controlling units has been an active area of research since past few decades. Abundant reports do exit in literature, which support a kind of 'cascading mechanism' for this purpose. It is now a well-established fact that a di ...
... mechanism of 'transfer' of this information from outer surface to the core controlling units has been an active area of research since past few decades. Abundant reports do exit in literature, which support a kind of 'cascading mechanism' for this purpose. It is now a well-established fact that a di ...
Can correct protein models be identified?
... first generation of decoys was generated by putting the sequence from a particular protein onto the backbone of another protein. Novotny studied different parameters distinguishing between models built on the native and an incorrect backbone (Novotny et al. 1988). Later, Sippl developed knowledge-ba ...
... first generation of decoys was generated by putting the sequence from a particular protein onto the backbone of another protein. Novotny studied different parameters distinguishing between models built on the native and an incorrect backbone (Novotny et al. 1988). Later, Sippl developed knowledge-ba ...
all plant protein - frequently asked questions
... soy flakes. The gentle process of extraction preserves the form of the protein while helping to extract anti-nutritional components such as oxylate and phytate. These compounds are water soluble and thus removed with the processing of the protein. 32. Is consuming too much NUTRILITE™ All Plant Prote ...
... soy flakes. The gentle process of extraction preserves the form of the protein while helping to extract anti-nutritional components such as oxylate and phytate. These compounds are water soluble and thus removed with the processing of the protein. 32. Is consuming too much NUTRILITE™ All Plant Prote ...
Evolution of Function in Protein Superfamilies, from a Structural
... between homologues based on an incorrect functional assignment would ultimately undermine the value of genome databases. There are two ways to help practically to improve genome analysis. Firstly, one can assess quantitatively the reliability of annotation transfer, and recent work has provided valu ...
... between homologues based on an incorrect functional assignment would ultimately undermine the value of genome databases. There are two ways to help practically to improve genome analysis. Firstly, one can assess quantitatively the reliability of annotation transfer, and recent work has provided valu ...
Biochemical and Molecular Characterization of the Chicken
... The LIM motif (Freyd et al., 1990), which exhibits the consensus amino acid sequence CX2CX,6_23HX2CX2CX2CXI6_21CX2_~(C,H,D)(Sadler et al., 1992), has been identified in a number of gene products (Table I), several of which have been proposed to function as transcription factors. The LIM motif was fi ...
... The LIM motif (Freyd et al., 1990), which exhibits the consensus amino acid sequence CX2CX,6_23HX2CX2CX2CXI6_21CX2_~(C,H,D)(Sadler et al., 1992), has been identified in a number of gene products (Table I), several of which have been proposed to function as transcription factors. The LIM motif was fi ...
PKC in limb development
... PKC Biochemical binding data demonstrate that RACK1 binds and stabilizes activated PKC (Ron et al., 1994; Rotenberg and Sun, 1998). RACK1 has particularly high affinity for PKCα and PKCβ; therefore, we examined by immunohistochemistry whether the activated form of PKC was also expressed in RACK1-pos ...
... PKC Biochemical binding data demonstrate that RACK1 binds and stabilizes activated PKC (Ron et al., 1994; Rotenberg and Sun, 1998). RACK1 has particularly high affinity for PKCα and PKCβ; therefore, we examined by immunohistochemistry whether the activated form of PKC was also expressed in RACK1-pos ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).