In vitro phosphorylation of the movement protein of tomato mosaic
... tabacum and tobacco suspension culture cells (BY-2) in the presence of [γ-32P]ATP. Phosphorylation occurred even after washing the beads with a detergent-containing buffer, indicating that the recombinant MP formed a stable complex with some protein kinase(s) during incubation with the cell extract. ...
... tabacum and tobacco suspension culture cells (BY-2) in the presence of [γ-32P]ATP. Phosphorylation occurred even after washing the beads with a detergent-containing buffer, indicating that the recombinant MP formed a stable complex with some protein kinase(s) during incubation with the cell extract. ...
Peptide microarrays for detailed, high-throughput
... Figure 2: Time course of PKA-catalyzed phosphorylation on the array for a number of peptides representing known protein phosphorylation substrates. The peptide ID is based on the UniProt Knowledgebase, and the numbers indicate the position of the first and last amino acids of the peptide in the comp ...
... Figure 2: Time course of PKA-catalyzed phosphorylation on the array for a number of peptides representing known protein phosphorylation substrates. The peptide ID is based on the UniProt Knowledgebase, and the numbers indicate the position of the first and last amino acids of the peptide in the comp ...
Design and Evolution of Artificial M13 Coat Proteins
... wild-type phage coat proteins were supplied by a helper phage. As a consequence, non-functional coat proteins could be selected, provided they incorporated into the wild-type coat without signi®cantly impairing the assembly process. While ACP-7 was evolved from completely synthetic sequences, there ...
... wild-type phage coat proteins were supplied by a helper phage. As a consequence, non-functional coat proteins could be selected, provided they incorporated into the wild-type coat without signi®cantly impairing the assembly process. While ACP-7 was evolved from completely synthetic sequences, there ...
Lecture Note 6
... sequence identity must be atleast 30% over the total length of the sequence. Proteins with structural or functional similarity but low sequence identity are classified into the superfamilies. Whereas proteins with similar secondary structure arrangement belongs to the fold. CATH-Similar to SCOP, CAT ...
... sequence identity must be atleast 30% over the total length of the sequence. Proteins with structural or functional similarity but low sequence identity are classified into the superfamilies. Whereas proteins with similar secondary structure arrangement belongs to the fold. CATH-Similar to SCOP, CAT ...
Advances in Amino Acid Analysis
... Ala, Leu, Lys, and Gly — are well recovered and commonly used for protein quantitation according to their residue yields. Actually, eight residues are well recovered because Asn and Gln are converted to Asp and Glu by deamidation under harsh acid conditions. ...
... Ala, Leu, Lys, and Gly — are well recovered and commonly used for protein quantitation according to their residue yields. Actually, eight residues are well recovered because Asn and Gln are converted to Asp and Glu by deamidation under harsh acid conditions. ...
1 Introduction - diss.fu
... Probably the first initiation factor, which binds to the ribosome, is IF3. It has two identified functions. (1) It acts as an anti-association factor preventing formation of the 70S ribosome from 50S and 30S subunits. (2) It plays an important role in the codon-anticodon discrimination at the P-site ...
... Probably the first initiation factor, which binds to the ribosome, is IF3. It has two identified functions. (1) It acts as an anti-association factor preventing formation of the 70S ribosome from 50S and 30S subunits. (2) It plays an important role in the codon-anticodon discrimination at the P-site ...
Gene Section GHRL (ghrelin/obestatin prepropeptide) Atlas of Genetics and Cytogenetics
... As the endogenous ligand of the growth hormone secretagogues (GHS) receptor, Ghrelin/MTLRP is involved in the pulsatile secretion of Growth hormone. In addition to this role Ghrelin/MTLRP is in the regulation of feeding. In rodent In contrast to leptin, Ghrelin/MTLRP promotes food intake and obesity ...
... As the endogenous ligand of the growth hormone secretagogues (GHS) receptor, Ghrelin/MTLRP is involved in the pulsatile secretion of Growth hormone. In addition to this role Ghrelin/MTLRP is in the regulation of feeding. In rodent In contrast to leptin, Ghrelin/MTLRP promotes food intake and obesity ...
The role of cytosolic proteins in the insertion of tail
... nascent chain to the Sec61 protein-conducting channel (Rapoport, 2007). The well characterized co-translational pathway is, however, not accessible to the large and diverse group of membrane proteins that are classified as tail-anchored (TA) proteins. TA proteins consist of a cytosolic N-terminal do ...
... nascent chain to the Sec61 protein-conducting channel (Rapoport, 2007). The well characterized co-translational pathway is, however, not accessible to the large and diverse group of membrane proteins that are classified as tail-anchored (TA) proteins. TA proteins consist of a cytosolic N-terminal do ...
An Abscisic Acid-Activated and Calcium-lndependent
... activity by an in-gel assay (see Methods). When the gel was incubated with y-32P-ATP in the presence of 100 \iM free Ca2+, two major bands at 57 and 48 kD were seen in soluble proteins from ABA-treated GCPs. However, only the 57-kD band could be detected in protein samples from GCPs treated with eth ...
... activity by an in-gel assay (see Methods). When the gel was incubated with y-32P-ATP in the presence of 100 \iM free Ca2+, two major bands at 57 and 48 kD were seen in soluble proteins from ABA-treated GCPs. However, only the 57-kD band could be detected in protein samples from GCPs treated with eth ...
How Much Protein? - Hammer Nutrition
... Research confirms that rate of recovery and muscle synthesis rate are dramatically increased if amino acids and carbohydrates is consumed together immediately after the exercise training. Intense training may increase dietary protein requirements to as high as 1.7 grams protein per kilogram bodyweig ...
... Research confirms that rate of recovery and muscle synthesis rate are dramatically increased if amino acids and carbohydrates is consumed together immediately after the exercise training. Intense training may increase dietary protein requirements to as high as 1.7 grams protein per kilogram bodyweig ...
Does a backwardly read protein sequence have a unique native state?
... and, in principle, they may not stabilize newly formed helices. Also, turn region sequences, when read backwards, will rarely be in agreement with the turn tendencies observed for real proteins (Wilmot and Thornton, 1988). However, retro-proteins constitute a very interesting case for the study of p ...
... and, in principle, they may not stabilize newly formed helices. Also, turn region sequences, when read backwards, will rarely be in agreement with the turn tendencies observed for real proteins (Wilmot and Thornton, 1988). However, retro-proteins constitute a very interesting case for the study of p ...
Training for Endurance and Strength: Lessons from Cell Signaling
... muscle's adaptation to exercise that may enable explanation of this phenomenon at the molecular level. As will be described in depth in the following four papers, two serine/threonine protein kinases in particular play a particularly important role in this process. Protein kinase B/Akt can both acti ...
... muscle's adaptation to exercise that may enable explanation of this phenomenon at the molecular level. As will be described in depth in the following four papers, two serine/threonine protein kinases in particular play a particularly important role in this process. Protein kinase B/Akt can both acti ...
Plant mitochondria contain the protein translocase subunits TatB
... The twin-arginine translocation (Tat) pathway differs from most other protein translocating systems in that it transports fully folded proteins (Lee et al., 2006). It is named after its targeting signal, which contains a pair of adjacent arginine residues (the twin arginines). Identified in all doma ...
... The twin-arginine translocation (Tat) pathway differs from most other protein translocating systems in that it transports fully folded proteins (Lee et al., 2006). It is named after its targeting signal, which contains a pair of adjacent arginine residues (the twin arginines). Identified in all doma ...
Proteomic analyses of the time course responses of mice infected
... using MALDI-TOF MS. The results of the protein identification analysis are shown in Table 3. These data revealed that multiple immunogenic proteins at different locations on 2DE-immunoblotting showed diverse Mr and pI values and were correlated with theoretical values. Evidence of the subcellular lo ...
... using MALDI-TOF MS. The results of the protein identification analysis are shown in Table 3. These data revealed that multiple immunogenic proteins at different locations on 2DE-immunoblotting showed diverse Mr and pI values and were correlated with theoretical values. Evidence of the subcellular lo ...
The Formation of the Central Element of the
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
Here. - Blumenstiel Lab
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
... extensive SC. The meiotic stages of leptotene and zygotene occur in early region 2a, followed by pachytene, in which a full SC has formed (Page and Hawley 2001). The oocyte remains in pachytene from region 2a through region 3 in the germarium, while the other cells that initially build the SC revert ...
Identification of surface proteins in Enterococcus - UiO
... to mucosal and epithelial surfaces have been reported [2]. In addition to involvement in adhesion, surface proteins may affect virulence in other ways, for example by involvement in cell-cell signalling [9], interactions with the host immune system, sensing environmental factors, or protection from ...
... to mucosal and epithelial surfaces have been reported [2]. In addition to involvement in adhesion, surface proteins may affect virulence in other ways, for example by involvement in cell-cell signalling [9], interactions with the host immune system, sensing environmental factors, or protection from ...
Macromolecules Biological Molecules Macromolecules
... Some Conditions can not break covalent bonds, but can upset the weaker noncovalent interactions that determine secondary and tertiary structure, may affect a protein's shape and thus its function Denaturation Couses: Increases in temperature , Alterations in pH, High concentrations of polar sub ...
... Some Conditions can not break covalent bonds, but can upset the weaker noncovalent interactions that determine secondary and tertiary structure, may affect a protein's shape and thus its function Denaturation Couses: Increases in temperature , Alterations in pH, High concentrations of polar sub ...
Systemic Delivery of siRNA by a Plant PHLOEM SMALL RNA
... (a) Negative control in which primer sets were omitted from the in situ RT-PCR mixture. IP and EP, internal and external phloem, respectively; X, xylem; CA, cambium. (b) Presence of CmPSRPK1 and (D) CmPP16 in phloem tissues. Note that yellowish (positive) signal is produced by merging the green sign ...
... (a) Negative control in which primer sets were omitted from the in situ RT-PCR mixture. IP and EP, internal and external phloem, respectively; X, xylem; CA, cambium. (b) Presence of CmPSRPK1 and (D) CmPP16 in phloem tissues. Note that yellowish (positive) signal is produced by merging the green sign ...
Development of a protein microarray using sequence
... the fluorescent dye Alexa Fluor 647 reactive dye, and was prepared in the binding buffer for the model experiment of protein–protein interaction. 400 lg/mL of GAL4 DBD/EGFP fusion protein was spotted on the slides. The protein-immobilized slides were incubated with the labeled antibody solution for 1 ...
... the fluorescent dye Alexa Fluor 647 reactive dye, and was prepared in the binding buffer for the model experiment of protein–protein interaction. 400 lg/mL of GAL4 DBD/EGFP fusion protein was spotted on the slides. The protein-immobilized slides were incubated with the labeled antibody solution for 1 ...
Differences in Total Mitochondrial Proteins and
... The incorporation of [35S]methionine into mitochondrial mas and their respective host livers is the absence or proteins of both normal liver and hepatomas was carried reduction of a polypeptide with an approximate molecular out in the presence of cycloheximide, an inhibitor of cytoweight of 36,000 i ...
... The incorporation of [35S]methionine into mitochondrial mas and their respective host livers is the absence or proteins of both normal liver and hepatomas was carried reduction of a polypeptide with an approximate molecular out in the presence of cycloheximide, an inhibitor of cytoweight of 36,000 i ...
Super secondary structure (Motif)
... 5. EF hand is two helices connected by a loop that contains residues to coordinate calcium ion (Ca2+) Name refers to the helices E and F in parvalbumin Loop contains 12 amino acids, 5 bind Ca++ ...
... 5. EF hand is two helices connected by a loop that contains residues to coordinate calcium ion (Ca2+) Name refers to the helices E and F in parvalbumin Loop contains 12 amino acids, 5 bind Ca++ ...
The Effects of Whey Supplementation and Natural Diet on Protein
... chapter examines multiple sources of protein from natural foods and compares their effects on the rate and magnitude of muscle tissue anabolism to that of supplemental protein sources. ...
... chapter examines multiple sources of protein from natural foods and compares their effects on the rate and magnitude of muscle tissue anabolism to that of supplemental protein sources. ...
Bladder Pharmacology Campbell-Walsh Ch. 56: 1948-1972
... cholinergic nerve terminal = Inhibiting striated and smooth muscle contractions Also shown to inhibit afferent nerve activity ...
... cholinergic nerve terminal = Inhibiting striated and smooth muscle contractions Also shown to inhibit afferent nerve activity ...
Comparison of Protein Active Site Structures for
... identical protein–ligand structures based on the number of structures found within the unit cell. Multiple binding sites within the same structure are identified and only one copy is maintained if the ligand-defined active sites share 80% sequence identity and bind the same ligand. Thus, the list m ...
... identical protein–ligand structures based on the number of structures found within the unit cell. Multiple binding sites within the same structure are identified and only one copy is maintained if the ligand-defined active sites share 80% sequence identity and bind the same ligand. Thus, the list m ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).