Powerpoint slides - School of Engineering and Applied Science
... Computational Modeling Researches have been working for decades to develop procedures for predicting protein structure that are not so time consuming and not hindered by size and solubility constrains. As protein sequences are encoded in DNA, in principle, it should therefore be possible to translat ...
... Computational Modeling Researches have been working for decades to develop procedures for predicting protein structure that are not so time consuming and not hindered by size and solubility constrains. As protein sequences are encoded in DNA, in principle, it should therefore be possible to translat ...
Does Plant Cell Death Require Toxin Entry?
... necessary for disease symptoms? 2. Where does the protein exert activity (i.e. where is the site-of-action)? ...
... necessary for disease symptoms? 2. Where does the protein exert activity (i.e. where is the site-of-action)? ...
Evidence for inducible recruitment of Wiskott
... family GTPase Cdc42.30 Thus, it has been suggested that the recruitment of WASp to the plasma membranes is mediated by the SLP-76 protein.14 In addition, molecular imaging analysis also reveals the co-localization of WASp with TCR-associated protein tyrosine kinase ZAP-70 (ζ-chain-associated protein ...
... family GTPase Cdc42.30 Thus, it has been suggested that the recruitment of WASp to the plasma membranes is mediated by the SLP-76 protein.14 In addition, molecular imaging analysis also reveals the co-localization of WASp with TCR-associated protein tyrosine kinase ZAP-70 (ζ-chain-associated protein ...
1 Evolutionary conservation and emerging functional diversity of the
... ~70 aa residues with a highly conserved and functionally critical histidine, proline and aspartic acid (HPD) tri-peptide motif. Although J domains are critical for J protein function, often the regions outside the J domain determine the interaction of J proteins with their clients or affect their su ...
... ~70 aa residues with a highly conserved and functionally critical histidine, proline and aspartic acid (HPD) tri-peptide motif. Although J domains are critical for J protein function, often the regions outside the J domain determine the interaction of J proteins with their clients or affect their su ...
Translocation of proteins across archaeal cytoplasmic membranes
... type IV pilin peptidase (Fig. 1(a)) [32]. Cleavage of this signal sequence thus retains the hydrophobic region as part of the mature protein which plays an essential role in the biogenesis of the pilus. The archaea synthesize their flagella (which more closely resemble bacterial pili) as preproteins ...
... type IV pilin peptidase (Fig. 1(a)) [32]. Cleavage of this signal sequence thus retains the hydrophobic region as part of the mature protein which plays an essential role in the biogenesis of the pilus. The archaea synthesize their flagella (which more closely resemble bacterial pili) as preproteins ...
Isolation by Calcium-Dependent Translocation to
... the defense against microbes. Phagocytosing neutrophils kill and degrade bacteria through the action of toxic oxygen metabolites and proteolytic enzymes.’.2Recognition of the microbes as well as phagolysosome formation, a prerequisite for killing, is dependent on secretion or degranulation of granul ...
... the defense against microbes. Phagocytosing neutrophils kill and degrade bacteria through the action of toxic oxygen metabolites and proteolytic enzymes.’.2Recognition of the microbes as well as phagolysosome formation, a prerequisite for killing, is dependent on secretion or degranulation of granul ...
Finding a way to the nucleus - Purdue University
... to T-strands in the plant, thus protecting the DNA from nucleolytic destruction [9,10]. The complex of T-strands covalently linked to VirD2 and coated by VirE2 molecules is termed the ‘T-complex’ [11]. Although there are strong genetic and in vitro binding data indicating the existence of the T-comp ...
... to T-strands in the plant, thus protecting the DNA from nucleolytic destruction [9,10]. The complex of T-strands covalently linked to VirD2 and coated by VirE2 molecules is termed the ‘T-complex’ [11]. Although there are strong genetic and in vitro binding data indicating the existence of the T-comp ...
TGF-beta SIGNAL TRANSDUCTION - University of Colorado-MCDB
... of structurally related polypeptide growth factors, each capable of regulating a fascinating array of cellular processes including cell proliferation, lineage determination, differentiation, motility, adhesion, and death. Expressed in complex temporal and tissue-specific patterns, TGF-β and related ...
... of structurally related polypeptide growth factors, each capable of regulating a fascinating array of cellular processes including cell proliferation, lineage determination, differentiation, motility, adhesion, and death. Expressed in complex temporal and tissue-specific patterns, TGF-β and related ...
Function and specificity of 14-3-3 proteins in the regulation of
... reduced in vitro by 5¢-AMP which interacts with 14-3-3s and directly reduces their binding to target ligands (Kaiser and Spill, 1991; Athwal et al., 1998b). It is noteworthy that there is evidence for an AMP binding site in a spinach 14-3-3 isoform. Thus 14-3-3 protein activity might also be under m ...
... reduced in vitro by 5¢-AMP which interacts with 14-3-3s and directly reduces their binding to target ligands (Kaiser and Spill, 1991; Athwal et al., 1998b). It is noteworthy that there is evidence for an AMP binding site in a spinach 14-3-3 isoform. Thus 14-3-3 protein activity might also be under m ...
The Methylosome, a 20S Complex Containing JBP1 and pICln
... transferred to the SMN complex in vitro. Together with previous results, these data indicate that methylation of Sm proteins by the methylosome directs Sm proteins to the SMN complex for assembly into snRNP core particles and suggest that the methylosome can regulate snRNP assembly. ters Sm proteins ...
... transferred to the SMN complex in vitro. Together with previous results, these data indicate that methylation of Sm proteins by the methylosome directs Sm proteins to the SMN complex for assembly into snRNP core particles and suggest that the methylosome can regulate snRNP assembly. ters Sm proteins ...
Milk Protein - AgroMedia International Inc
... Supplemental fats are known to depress milk protein. When milk protein is depressed by supplemental fat, the addition of ruminally protected lysine and methionine have not alleviated the protein depression caused by fat. The benefits of increasing milk production with fat supplements should be weigh ...
... Supplemental fats are known to depress milk protein. When milk protein is depressed by supplemental fat, the addition of ruminally protected lysine and methionine have not alleviated the protein depression caused by fat. The benefits of increasing milk production with fat supplements should be weigh ...
Nuclear Transport of Plant Potyviral Proteins
... and cytoplasmic localizationof nonfused GUS. These results indicate that both Nla and Nlb contain nuclear targeting signals, and that they may serve as useful models for studies of plant cell nuclear transport. A discussion of the general utility of the nuclear transport system described here, as we ...
... and cytoplasmic localizationof nonfused GUS. These results indicate that both Nla and Nlb contain nuclear targeting signals, and that they may serve as useful models for studies of plant cell nuclear transport. A discussion of the general utility of the nuclear transport system described here, as we ...
Review Structural glycobiology: A game of snakes and ladders
... from its starting conformation (either 1 C4 or 0 S2 ) through to the transition state (3 E): 1 C4 → 3 H2 → 0 S2 → 3,0 B → 3 S1 → 3 E (Mulakala et al. 2006). Computational docking methods have evolved to help overcome difficulties in obtaining experimental 3D structures of multiple ligands to a commo ...
... from its starting conformation (either 1 C4 or 0 S2 ) through to the transition state (3 E): 1 C4 → 3 H2 → 0 S2 → 3,0 B → 3 S1 → 3 E (Mulakala et al. 2006). Computational docking methods have evolved to help overcome difficulties in obtaining experimental 3D structures of multiple ligands to a commo ...
Development of an Assay for the Identification of Receptor Binding
... 100 or 200 ng of sheared phage DNA in a 20 µL reaction volume overnight at 16 ˝ C, and the ligation mixture was transformed into chemically competent Top10 cells (Invitrogen). The resulting colonies were then screened for the presence of inserted DNA into pET30a. If at least 2/10 verified plasmids c ...
... 100 or 200 ng of sheared phage DNA in a 20 µL reaction volume overnight at 16 ˝ C, and the ligation mixture was transformed into chemically competent Top10 cells (Invitrogen). The resulting colonies were then screened for the presence of inserted DNA into pET30a. If at least 2/10 verified plasmids c ...
31P n.m.r. analysis of the renal response to respiratory acidosis
... Marchand, 1971; Ord & Stocken, 1981) and compensatory growth after partial hepatectomy (Witschi, 1970) are all inhibited in vivo. The kinase sensitive to Be2+ has now been identified as a cyclic AMP-independent enzyme which preVOl. ...
... Marchand, 1971; Ord & Stocken, 1981) and compensatory growth after partial hepatectomy (Witschi, 1970) are all inhibited in vivo. The kinase sensitive to Be2+ has now been identified as a cyclic AMP-independent enzyme which preVOl. ...
Pharmacolecture 12 - pharmacology1lecnotes
... The actions of histamine are mediated by at least three distinct receptors: H1, H2, and H3. Of these, the H1 and H2 receptors are the best characterized and mediated welldefined response in humans that have been used as the basis for drug development. Response such as bronchoconstriction are mediat ...
... The actions of histamine are mediated by at least three distinct receptors: H1, H2, and H3. Of these, the H1 and H2 receptors are the best characterized and mediated welldefined response in humans that have been used as the basis for drug development. Response such as bronchoconstriction are mediat ...
The Calcium-Binding Activity of a Vacuole
... A vacuole membrane-associated calcium-binding protein with an apparent mass of 45 kD was purified from celery (Apium graveolens). This protein, VCaB45, is enriched in highly vacuolate tissues and is located within the lumen of vacuoles. Antigenically related proteins are present in many dicotyledono ...
... A vacuole membrane-associated calcium-binding protein with an apparent mass of 45 kD was purified from celery (Apium graveolens). This protein, VCaB45, is enriched in highly vacuolate tissues and is located within the lumen of vacuoles. Antigenically related proteins are present in many dicotyledono ...
Folie 1 - FLI
... • Assign all of the residues in the peptide the appropriate set of parameters. • Scan through the peptide and identify regions where 4 out of 6 contiguous residues have P(a-helix) > 100. • That region is declared an alpha-helix. Extend the helix in both directions until a set of four contiguous resi ...
... • Assign all of the residues in the peptide the appropriate set of parameters. • Scan through the peptide and identify regions where 4 out of 6 contiguous residues have P(a-helix) > 100. • That region is declared an alpha-helix. Extend the helix in both directions until a set of four contiguous resi ...
Role of N-terminal protein formylation in central metabolic processes
... Full list of author information is available at the end of the article ...
... Full list of author information is available at the end of the article ...
The arbuscular mycorrhizal fungal protein glomalin is
... other fungi (Fig. 2) was identified from a G. intraradices cDNA library. Using CLUSTAL X (Thompson et al., 1997), an unrooted phylogenetic tree was constructed based on the full-length amino acid sequence of Hsp 60 ORF from various organisms (Fig. 3). The resulting tree shows that the GiHsp 60 is in ...
... other fungi (Fig. 2) was identified from a G. intraradices cDNA library. Using CLUSTAL X (Thompson et al., 1997), an unrooted phylogenetic tree was constructed based on the full-length amino acid sequence of Hsp 60 ORF from various organisms (Fig. 3). The resulting tree shows that the GiHsp 60 is in ...
Qproteome Nuclear Protein Handbook
... cell biologists. The identification of nuclear proteins — especially nucleic-acid– binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s ge ...
... cell biologists. The identification of nuclear proteins — especially nucleic-acid– binding proteins (e.g., transcription factors) — is important for an understanding of genome regulation and function, and provides clues about the molecular function of novel proteins. The nucleus contains a cell’s ge ...
Predicting Secondary Structures of Proteins
... four structural levels, but only the first two levels are useful for achieving the goal of the analysis described in this article. The primary structure of a protein is the sequence of amino acids in the polypeptide chain; it can be represented as a string on the finite alphabet aa, with | aa| = ...
... four structural levels, but only the first two levels are useful for achieving the goal of the analysis described in this article. The primary structure of a protein is the sequence of amino acids in the polypeptide chain; it can be represented as a string on the finite alphabet aa, with | aa| = ...
Guanine Nucleotide Exchange-Independent Activation of Gs Protein
... 1 M GTP in membranes prepared from cyc⫺ or HEK-293 cells expressing the fusion protein. Untransfected control cell membranes exhibited no agonist-mediated stimulation of cyclase activity. Considering that GTP is the activating nucleotide for G proteins and that G protein activation is achieved by r ...
... 1 M GTP in membranes prepared from cyc⫺ or HEK-293 cells expressing the fusion protein. Untransfected control cell membranes exhibited no agonist-mediated stimulation of cyclase activity. Considering that GTP is the activating nucleotide for G proteins and that G protein activation is achieved by r ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).